(Circular) (Six-base) MAPSORT of: pFN21AB5875.seq Check: 1381 from: 1 to: 6100 pFN21AB5875 6100 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3910 278 Size: 53 83 186 936 2374 2468 Fragments arranged by size: 2468 2374 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3443 1937 Size: 1506 4594 Acc65I G'GTAC_C Cuts at: 3421 3421 Size: 6100 AclI AA'CG_TT Cuts at: 1563 2673 4227 4600 1563 Size: 1110 1554 373 3063 Fragments arranged by size: 3063 1554 1110 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4163 5547 5815 1632 Size: 2531 1384 268 1917 Fragments arranged by size: 2531 1917 1384 268 AfeI AGC'GCT Cuts at: 2358 2358 Size: 6100 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5903 AflIII A'CryG_T Cuts at: 1203 4999 5976 1203 Size: 3796 977 1327 Fragments arranged by size: 3796 1327 977 AgeI A'CCGG_T Cuts at: 4931 4931 Size: 6100 AhdI GACnn_n'nnGTC Cuts at: 4829 4829 Size: 6100 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6100 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6100 AlwNI CAG_nnn'CTG Cuts at: 2041 2584 3843 5415 2041 Size: 543 1259 1572 2726 Fragments arranged by size: 2726 1572 1259 543 ApaI G_GGCC'C Cuts at: 3039 3039 Size: 6100 ApaLI G'TGCA_C Cuts at: 4157 5313 4157 Size: 1156 4944 ApoI r'AATT_y Cuts at: 1493 3243 3409 3635 3787 5896 1493 Size: 1750 166 226 152 2109 1697 Fragments arranged by size: 2109 1750 1697 226 166 152 AseI AT'TA_AT Cuts at: 160 1032 4654 160 Size: 872 3622 1606 Fragments arranged by size: 3622 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 AvaI C'yCGr_G Cuts at: 1870 1943 2164 3317 3425 1870 Size: 73 221 1153 108 4545 Fragments arranged by size: 4545 1153 221 108 73 BamHI G'GATC_C Cuts at: 3430 3797 3430 Size: 367 5733 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2234 3421 4876 618 Size: 325 217 627 447 1187 1455 1842 Fragments arranged by size: 1842 1455 1187 627 447 325 217 BanII G_rGCy'C Cuts at: 1391 2802 3039 3419 1391 Size: 1411 237 380 4072 Fragments arranged by size: 4072 1411 380 237 BbeI G_GCGC'C Cuts at: 2238 2238 Size: 6100 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3390 928 Size: 971 1491 3638 Fragments arranged by size: 3638 1491 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4291 4325 1903 Size: 34 2354 34 3678 Fragments arranged by size: 3678 2354 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3992 5202 3992 Size: 1210 4890 BclI T'GATC_A Cuts at: 1548 1548 Size: 6100 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4711 136 Size: 107 122 71 631 605 3039 1525 Fragments arranged by size: 3039 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6095 6095 Size: 6100 BlpI GC'TnA_GC Cuts at: 3517 3517 Size: 6100 Bme1580I G_kGCm'C Cuts at: 1790 2116 2269 3039 4161 5317 1790 Size: 326 153 770 1122 1156 2573 Fragments arranged by size: 2573 1156 1122 770 326 153 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6100 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2256 2761 3831 Size: 416 53 83 399 24 839 505 1070 Cuts at: 3831 4789 442 Size: 958 1753 Fragments arranged by size: 1753 1070 958 839 505 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2123 1056 Size: 1067 5033 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2051 2308 2432 2952 4760 1239 Size: 812 257 124 520 1808 2579 Fragments arranged by size: 2579 1808 812 520 257 124 Bpu10I CC'TnA_GC Cuts at: 2585 3311 2585 Size: 726 5374 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4225 5090 5388 4225 Size: 865 298 4937 Fragments arranged by size: 4937 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4763 882 Size: 617 3264 2219 Fragments arranged by size: 3264 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3891 493 Size: 711 2687 2702 Fragments arranged by size: 2702 2687 711 BsaBI GATnn'nnATC Cuts at: 6094 6094 Size: 6100 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2235 3907 4289 4924 Size: 53 83 186 936 702 1672 382 635 Cuts at: 4924 275 Size: 1451 Fragments arranged by size: 1672 1451 936 702 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4533 4931 5205 5352 5763 4533 Size: 398 274 147 411 4870 Fragments arranged by size: 4870 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2306 2336 1178 Size: 30 165 30 903 30 4942 Fragments arranged by size: 4942 903 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2209 2246 2288 3189 3338 1186 Size: 1023 37 42 901 149 3948 Fragments arranged by size: 3948 1023 901 149 42 37 BseYI C'CCAG_C Cuts at: 2359 3211 5303 5799 2359 Size: 852 2092 496 2660 Fragments arranged by size: 2660 2092 852 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2466 2827 1163 Size: 573 730 361 4436 Fragments arranged by size: 4436 730 573 361 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3550 4311 5339 1540 Size: 275 186 1549 761 1028 2301 Fragments arranged by size: 2301 1549 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2802 4161 4246 5317 2802 Size: 1359 85 1071 3585 Fragments arranged by size: 3585 1359 1071 85 BsmI GAATG_Cn' Cuts at: 3611 3704 3611 Size: 93 6007 BsmBI CGTCTCn'nnnn_ Cuts at: 2553 2553 Size: 6100 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2116 2269 2802 3039 3419 4161 4246 Size: 399 326 153 533 237 380 742 85 Cuts at: 4246 5317 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 533 399 380 326 237 153 85 BspEI T'CCGG_A Cuts at: 5763 5763 Size: 6100 BspHI T'CATG_A Cuts at: 3989 5719 3989 Size: 1730 4370 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3455 844 Size: 2611 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2027 3987 1394 Size: 429 204 1960 3507 Fragments arranged by size: 3507 1960 429 204 BsrDI GCAATG_nn' Cuts at: 65 1248 4595 4769 65 Size: 1183 3347 174 1396 Fragments arranged by size: 3347 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2246 2288 4744 4931 1700 Size: 110 436 42 2456 187 2869 Fragments arranged by size: 2869 2456 436 187 110 42 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6100 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6100 BssSI C'ACGA_G Cuts at: 4160 5172 4160 Size: 1012 5088 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6100 BstBI TT'CG_AA Cuts at: 2917 4904 2917 Size: 1987 4113 BstEII G'GTnAC_C Cuts at: 1184 2044 1184 Size: 860 5240 BstXI CCAn_nnnn'nTGG Cuts at: 2677 3142 2677 Size: 465 5635 BstYI r'GATC_y Cuts at: 1972 2181 3430 3797 4182 4199 5651 5737 5749 Size: 209 1249 367 385 17 1452 86 12 Cuts at: 5749 6095 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1249 385 367 346 209 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2100 513 Size: 552 1035 4513 Fragments arranged by size: 4513 1035 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5361 Fragments arranged by size: 5361 683 56 BtsI GCAGTG_nn' Cuts at: 2114 3616 4410 4430 2114 Size: 1502 794 20 3784 Fragments arranged by size: 3784 1502 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6100 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6065 DraI TTT'AAA Cuts at: 2454 3404 2454 Size: 950 5150 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5107 809 Size: 507 3791 1802 Fragments arranged by size: 3791 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3547 4436 6065 Size: 54 1425 50 275 18 1717 889 1629 Cuts at: 6065 8 Size: 43 Fragments arranged by size: 1717 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3547 1537 Size: 275 1735 4090 Fragments arranged by size: 4090 1735 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2470 2805 3057 3350 4030 1353 Size: 1117 335 252 293 680 3423 Fragments arranged by size: 3423 1117 680 335 293 252 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4687 5061 5207 387 Size: 1064 138 3098 374 146 1280 Fragments arranged by size: 3098 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2800 2800 Size: 6100 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2308 2432 2952 4163 4760 5547 Size: 393 419 257 124 520 1211 597 787 Cuts at: 5547 5815 1239 Size: 268 1524 Fragments arranged by size: 1524 1211 787 597 520 419 393 268 257 124 EcoNI CCTnn'n_nnAGG Cuts at: 3320 3320 Size: 6100 EcoO109I rG'GnC_Cy Cuts at: 3035 3035 Size: 6100 EcoRI G'AATT_C Cuts at: 3409 3787 3409 Size: 378 5722 EcoRV GAT'ATC Cuts at: 2665 2665 Size: 6100 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2910 2910 Size: 6100 FspI TGC'GCA Cuts at: 4606 4606 Size: 6100 HaeII r_GCGC'y Cuts at: 1678 2238 2360 5247 1678 Size: 560 122 2887 2531 Fragments arranged by size: 2887 2531 560 122 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4741 4773 4815 4847 1373 Size: 32 135 32 3169 32 42 32 2626 Fragments arranged by size: 3169 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2865 3444 3690 669 Size: 988 281 927 579 246 3079 Fragments arranged by size: 3079 988 927 579 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6100 HpaI GTT'AAC Cuts at: 2865 3690 2865 Size: 825 5275 KasI G'GCGC_C Cuts at: 2234 2234 Size: 6100 KpnI G_GTAC'C Cuts at: 3425 3425 Size: 6100 MfeI C'AATT_G Cuts at: 3699 3699 Size: 6100 MluI A'CGCG_T Cuts at: 5976 5976 Size: 6100 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3276 5214 5398 1305 Size: 1971 1938 184 2007 Fragments arranged by size: 2007 1971 1938 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4278 Fragments arranged by size: 4278 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4058 4417 4576 518 Size: 648 2892 359 159 2042 Fragments arranged by size: 2892 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2090 2153 2376 3516 4193 5341 5586 1606 Size: 484 63 223 1140 677 1148 245 2120 Fragments arranged by size: 2120 1148 1140 677 484 245 223 63 NaeI GCC'GGC Cuts at: 2248 2290 2248 Size: 42 6058 NarI GG'CG_CC Cuts at: 2235 2235 Size: 6100 NcoI C'CATG_G Cuts at: 513 1065 2100 513 Size: 552 1035 4513 Fragments arranged by size: 4513 1035 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6100 NgoMIV G'CCGG_C Cuts at: 2246 2288 2246 Size: 42 6058 NheI G'CTAG_C Cuts at: 1052 2119 1052 Size: 1067 5033 NotI GC'GGCC_GC Cuts at: 3547 3547 Size: 6100 NspI r_CATG'y Cuts at: 1132 1229 2761 3458 5003 1132 Size: 97 1532 697 1545 2229 Fragments arranged by size: 2229 1545 1532 697 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6100 PciI A'CATG_T Cuts at: 4999 4999 Size: 6100 PflMI CCAn_nnn'nTGG Cuts at: 1108 2728 1108 Size: 1620 4480 PmeI GTTT'AAAC Cuts at: 3404 3404 Size: 6100 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4169 4201 5682 5714 4169 Size: 32 1481 32 4555 Fragments arranged by size: 4555 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6100 PsiI TTA'TAA Cuts at: 3670 3670 Size: 6100 PspOMI G'GGCC_C Cuts at: 3035 3035 Size: 6100 PstI C_TGCA'G Cuts at: 830 2095 2346 2738 3452 830 Size: 1265 251 392 714 3478 Fragments arranged by size: 3478 1265 714 392 251 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6100 SacI G_AGCT'C Cuts at: 2802 2802 Size: 6100 SalI G'TCGA_C Cuts at: 1936 3442 1936 Size: 1506 4594 SapI GCTCTTCn'nnn_ Cuts at: 2470 2470 Size: 6100 SbfI CC_TGCA'GG Cuts at: 3452 3452 Size: 6100 ScaI AGT'ACT Cuts at: 4348 6060 4348 Size: 1712 4388 SexAI A'CCwGG_T Cuts at: 2040 2040 Size: 6100 SfcI C'TryA_G Cuts at: 826 1045 2091 2342 2734 3448 4583 5264 5455 Size: 219 1046 251 392 714 1135 681 191 Cuts at: 5455 826 Size: 1471 Fragments arranged by size: 1471 1135 1046 714 681 392 251 219 191 SfoI GGC'GCC Cuts at: 2236 2236 Size: 6100 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6100 SmaI CCC'GGG Cuts at: 1872 2166 3427 1872 Size: 294 1261 4545 Fragments arranged by size: 4545 1261 294 SmlI C'TyrA_G Cuts at: 820 1017 1943 4204 5105 5367 820 Size: 197 926 2261 901 262 1553 Fragments arranged by size: 2261 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3891 493 Size: 3398 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6100 SphI G_CATG'C Cuts at: 1132 2761 3458 1132 Size: 1629 697 3774 Fragments arranged by size: 3774 1629 697 SspI AAT'ATT Cuts at: 5 52 4024 5 Size: 47 3972 2081 Fragments arranged by size: 3972 2081 47 StuI AGG'CCT Cuts at: 1517 2778 1517 Size: 1261 4839 StyI C'CwwG_G Cuts at: 513 1065 2100 2463 3031 3539 513 Size: 552 1035 363 568 508 3074 Fragments arranged by size: 3074 1035 568 552 508 363 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2810 4127 4144 4297 4482 4927 325 Size: 1255 1230 1317 17 153 185 445 1498 Fragments arranged by size: 1498 1317 1255 1230 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4346 6058 96 Size: 274 80 33 51 1443 2369 1712 138 Fragments arranged by size: 2369 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2306 2338 3255 3287 6060 6092 2306 Size: 32 917 32 2773 32 2314 Fragments arranged by size: 2773 2314 917 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6100 XbaI T'CTAG_A Cuts at: 3436 3436 Size: 6100 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2597 3107 2597 Size: 510 5590 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6100 XmaI C'CCGG_G Cuts at: 1870 2164 3425 1870 Size: 294 1261 4545 Fragments arranged by size: 4545 1261 294 XmnI GAAnn'nnTTC Cuts at: 3193 4229 3193 Size: 1036 5064 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3908 276 Size: 53 83 186 936 2374 2468 Fragments arranged by size: 2468 2374 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SfiI SrfI SwaI