(Linear) (Six-base) MAPSORT of: KIBB5875.seq Check: 3538 from: 1 to: 1395 KIBB5875.orf With 182 enzymes: SgfI * July 3, 2012 16:10 .. AclI AA'CG_TT Cuts at: 0 668 1395 Size: 668 727 AfeI AGC'GCT Cuts at: 0 353 1395 Size: 353 1042 AlwNI CAG_nnn'CTG Cuts at: 0 36 579 1395 Size: 36 543 816 Fragments arranged by size: 816 543 36 ApaI G_GGCC'C Cuts at: 0 1034 1395 Size: 1034 361 ApoI r'AATT_y Cuts at: 0 1238 1395 Size: 1238 157 AvaI C'yCGr_G Cuts at: 0 159 1312 1395 Size: 159 1153 83 Fragments arranged by size: 1153 159 83 BanI G'GyrC_C Cuts at: 0 229 1395 Size: 229 1166 BanII G_rGCy'C Cuts at: 0 797 1034 1395 Size: 797 237 361 Fragments arranged by size: 797 361 237 BbeI G_GCGC'C Cuts at: 0 233 1395 Size: 233 1162 BbsI GAAGACnn'nnnn_ Cuts at: 0 1385 1395 Size: 1385 10 Bme1580I G_kGCm'C Cuts at: 0 111 264 1034 1395 Size: 111 153 770 361 Fragments arranged by size: 770 361 153 111 BmrI ACTGGGnnnn_n' Cuts at: 0 251 756 1395 Size: 251 505 639 Fragments arranged by size: 639 505 251 BmtI G_CTAG'C Cuts at: 0 118 1395 Size: 118 1277 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 46 303 427 947 1395 Size: 46 257 124 520 448 Fragments arranged by size: 520 448 257 124 46 Bpu10I CC'TnA_GC Cuts at: 0 580 1306 1395 Size: 580 726 89 Fragments arranged by size: 726 580 89 BsaHI Gr'CG_yC Cuts at: 0 230 1395 Size: 230 1165 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 301 331 1395 Size: 301 30 1064 Fragments arranged by size: 1064 301 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 204 241 283 1184 1333 1395 Size: 204 37 42 901 149 62 Fragments arranged by size: 901 204 149 62 42 37 BseYI C'CCAG_C Cuts at: 0 354 1206 1395 Size: 354 852 189 Fragments arranged by size: 852 354 189 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 461 822 1395 Size: 461 361 573 Fragments arranged by size: 573 461 361 BsiHKAI G_wGCw'C Cuts at: 0 797 1395 Size: 797 598 BsmBI CGTCTCn'nnnn_ Cuts at: 0 548 1395 Size: 548 847 Bsp1286I G_dGCh'C Cuts at: 0 111 264 797 1034 1395 Size: 111 153 533 237 361 Fragments arranged by size: 533 361 237 153 111 BsrBI CCG'CTC Cuts at: 0 22 1395 Size: 22 1373 BsrFI r'CCGG_y Cuts at: 0 241 283 1395 Size: 241 42 1112 Fragments arranged by size: 1112 241 42 BstBI TT'CG_AA Cuts at: 0 912 1395 Size: 912 483 BstEII G'GTnAC_C Cuts at: 0 39 1395 Size: 39 1356 BstXI CCAn_nnnn'nTGG Cuts at: 0 672 1137 1395 Size: 672 465 258 Fragments arranged by size: 672 465 258 BstYI r'GATC_y Cuts at: 0 176 1395 Size: 176 1219 BtgI C'CryG_G Cuts at: 0 95 1395 Size: 95 1300 BtsI GCAGTG_nn' Cuts at: 0 109 1395 Size: 109 1286 DraI TTT'AAA Cuts at: 0 449 1395 Size: 449 946 EarI CTCTTCn'nnn_ Cuts at: 0 465 800 1052 1345 1395 Size: 465 335 252 293 50 Fragments arranged by size: 465 335 293 252 50 EcoICRI GAG'CTC Cuts at: 0 795 1395 Size: 795 600 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 46 303 427 947 1395 Size: 46 257 124 520 448 Fragments arranged by size: 520 448 257 124 46 EcoNI CCTnn'n_nnAGG Cuts at: 0 1315 1395 Size: 1315 80 EcoO109I rG'GnC_Cy Cuts at: 0 1030 1395 Size: 1030 365 EcoRV GAT'ATC Cuts at: 0 660 1395 Size: 660 735 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 905 1395 Size: 905 490 HaeII r_GCGC'y Cuts at: 0 233 355 1395 Size: 233 122 1040 Fragments arranged by size: 1040 233 122 HincII GTy'rAC Cuts at: 0 860 1395 Size: 860 535 HpaI GTT'AAC Cuts at: 0 860 1395 Size: 860 535 KasI G'GCGC_C Cuts at: 0 229 1395 Size: 229 1166 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1271 1395 Size: 1271 124 MspA1I CmG'CkG Cuts at: 0 85 148 371 1395 Size: 85 63 223 1024 Fragments arranged by size: 1024 223 85 63 NaeI GCC'GGC Cuts at: 0 243 285 1395 Size: 243 42 1110 Fragments arranged by size: 1110 243 42 NarI GG'CG_CC Cuts at: 0 230 1395 Size: 230 1165 NcoI C'CATG_G Cuts at: 0 95 1395 Size: 95 1300 NgoMIV G'CCGG_C Cuts at: 0 241 283 1395 Size: 241 42 1112 Fragments arranged by size: 1112 241 42 NheI G'CTAG_C Cuts at: 0 114 1395 Size: 114 1281 NspI r_CATG'y Cuts at: 0 756 1395 Size: 756 639 PflMI CCAn_nnn'nTGG Cuts at: 0 723 1395 Size: 723 672 PspOMI G'GGCC_C Cuts at: 0 1030 1395 Size: 1030 365 PstI C_TGCA'G Cuts at: 0 90 341 733 1395 Size: 90 251 392 662 Fragments arranged by size: 662 392 251 90 SacI G_AGCT'C Cuts at: 0 797 1395 Size: 797 598 SapI GCTCTTCn'nnn_ Cuts at: 0 465 1395 Size: 465 930 SexAI A'CCwGG_T Cuts at: 0 35 1395 Size: 35 1360 SfcI C'TryA_G Cuts at: 0 86 337 729 1395 Size: 86 251 392 666 Fragments arranged by size: 666 392 251 86 SfoI GGC'GCC Cuts at: 0 231 1395 Size: 231 1164 SmaI CCC'GGG Cuts at: 0 161 1395 Size: 161 1234 SphI G_CATG'C Cuts at: 0 756 1395 Size: 756 639 StuI AGG'CCT Cuts at: 0 773 1395 Size: 773 622 StyI C'CwwG_G Cuts at: 0 95 458 1026 1395 Size: 95 363 568 369 Fragments arranged by size: 568 369 363 95 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 805 1395 Size: 805 590 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 301 333 1250 1282 1395 Size: 301 32 917 32 113 Fragments arranged by size: 917 301 113 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 592 1102 1395 Size: 592 510 293 Fragments arranged by size: 592 510 293 XmaI C'CCGG_G Cuts at: 0 159 1395 Size: 159 1236 XmnI GAAnn'nnTTC Cuts at: 0 1188 1395 Size: 1188 207 Enzymes that do cut: AclI AfeI AlwNI ApaI ApoI AvaI BanI BanII BbeI BbsI Bme1580I BmrI BmtI BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BsrBI BsrFI BstBI BstEII BstXI BstYI BtgI BtsI DraI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII HincII HpaI KasI MmeI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PflMI PspOMI PstI SacI SapI SexAI SfcI SfoI SmaI SphI StuI StyI TaqII TstI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AcuI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI BstAPI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EaeI EagI EciI EcoRI FseI FspI FspAI Hin4I HindIII KpnI MfeI MluI MscI MslI NdeI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI SwaI TatI Tth111I XbaI XhoI ZraI