################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## AatII AccI AccII AccIII AcyI AflII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AspI AvaI AvrII BamHI BbeI BbrPI BcgI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FbaI FseI HaeII HgaI Hin1I KasI KspI MamI MluI MroI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PvuI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 1975 GGTACC G^GTACC AclI 1883 AACGTT AA^CGTT AflIII 1152 ACATGT A^C[GA][CT]GT AhdI 65 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp718I 1975 GGTACC G^GTACC AspEI 65 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 78 GTGCTC G[AT]GC[AT]^C AviII 1425 TGCGCA TGC^GCA BanII 1724 GAGCCC G[GA]GC[CT]^C BbsI 16 GTCTTC GAAGAC(2/6) BbvCI 124 CCTCAGC CCTCAGC(-5/-2) BciVI 1922 GGATAC GTATCC(6/5) BcnI 571 CCGGG CC^[GC]GG BglII 585 AGATCT A^GATCT BpuAI 16 GTCTTC GAAGAC(2/6) BsgI 133 CTGCAC GTGCAG(16/14) BsiHKAI 78 GTGCTC G[AT]GC[AT]^C BspHI 447 TCATGA T^CATGA BspLU11I 1152 ACATGT A^CATGT BstAPI 2175 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1131 CCAGTACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 1426 GCGC GCG^C DraI 2062 TTTAAA TTT^AAA DraII 947 AGGACCT [GA]G^G[ACGT]CC[CT] DraIII 111 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 384 GACTGCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 65 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1694 CTCTTC CTCTTC(1/4) EcoO109I 947 AGGACCT [GA]G^G[ACGT]CC[CT] EcoT22I 1910 ATGCAT ATGCA^T FauI 158 GCGGG CCCGC(4/6) FspI 1425 TGCGCA TGC^GCA HhaI 1426 GCGC GCG^C HinP1I 1426 GCGC G^CGC HindIII 418 AAGCTT A^AGCTT HpaI 1506 GTTAAC GTT^AAC KpnI 1975 GGTACC GGTAC^C Ksp632I 1694 CTCTTC CTCTTC(1/4) MfeI 967 CAATTG C^AATTG MunI 967 CAATTG C^AATTG NciI 571 CCGGG CC^[GC]GG NdeI 2135 CATATG CA^TATG NsiI 1910 ATGCAT ATGCA^T PleI 2255 GACTC GAGTC(4/5) PpuMI 947 AGGACCT [GA]G^G[AT]CC[CT] Psp1406I 1883 AACGTT AA^CGTT RcaI 447 TCATGA T^CATGA SexAI 1971 ACCAGGT A^CC[AT]GGT SmlI 1033 CTCAAG C^T[CT][GA]AG TaqI 633 TCGA T^CGA TthHB8I 633 TCGA T^CGA XbaI 1194 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 40 enzymes) # ################################### AlwI 1872 GATCC GGATC(4/5) 1465 GGATC AseI 847 ATTAAT AT^TAAT 1759 ATTAAT AsnI 847 ATTAAT AT^TAAT 1759 ATTAAT BalI 1472 TGGCCA TGG^CCA 2167 TGGCCA BanI 1672 GGCACC G^G[CT][GA]CC 1975 GGTACC BbvI 1160 GCAGC GCAGC(8/12) 1428 GCAGC BmyI 78 GTGCTC G[AGT]GC[ACT]^C 1724 GAGCCC BpmI 880 CTCCAG CTGGAG(16/14) 325 CTGGAG BsaI 1563 GAGACC GGTCTC(1/5) 203 GGTCTC BsaJI 84 CCTTGG C^C[ACGT][ACGT]GG 318 CCAAGG Bsp1286I 78 GTGCTC G[AGT]GC[ACT]^C 1724 GAGCCC BspMI 467 ACCTGC ACCTGC(4/8) 1255 ACCTGC BsrDI 306 GCAATG GCAATG(2/0) 388 GCAATG BstNI 1567 CCTGG CC^[AT]GG 1972 CCAGG BstYI 585 AGATCT [GA]^GATC[CT] 1465 GGATCT EcoRII 1567 CCTGG ^CC[AT]GG 1972 CCAGG EcoT14I 84 CCTTGG C^C[AT][AT]GG 318 CCAAGG HapII 571 CCGG C^CGG 2203 CCGG HincII 728 GTCAAC GT[CT]^[GA]AC 1506 GTTAAC HindII 728 GTCAAC GT[CT]^[GA]AC 1506 GTTAAC HpaII 571 CCGG C^CGG 2203 CCGG MaeII 695 ACGT A^CGT 1884 ACGT MflI 585 AGATCT [GA]^GATC[CT] 1465 GGATCT MluNI 1472 TGGCCA TGG^CCA 2167 TGGCCA MscI 1472 TGGCCA TGG^CCA 2167 TGGCCA MspA1I 1161 CAGCTG C[AC]G^C[GT]G 1429 CAGCTG MspI 571 CCGG C^CGG 2203 CCGG MvaI 1567 CCTGG CC^[AT]GG 1972 CCAGG PflMI 318 CCAAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 882 CCAGAAACTGG PshBI 847 ATTAAT AT^TAAT 1759 ATTAAT PvuII 1161 CAGCTG CAG^CTG 1429 CAGCTG ScaI 1133 AGTACT AGT^ACT 1444 AGTACT SfaNI 1909 GATGC GCATC(5/9) 2181 GATGC SphI 1283 GCATGC GCATG^C 1422 GCATGC SspI 44 AATATT AAT^ATT 221 AATATT StyI 84 CCTTGG C^C[AT][AT]GG 318 CCAAGG TseI 1160 GCAGC G^C[AT]GC 1428 GCAGC Van91I 318 CCAAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 882 CCAGAAACTGG XcmI 739 CCAAGACTACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2143 CCATCATTCATCTGG XhoII 585 AGATCT [GA]^GATC[CT] 1465 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AcsI AfaI AluI AlwNI ApoI Asp700I AvaII BfaI BsiYI BslI BsmAI BsrI BssKI BstF5I Cac8I Cfr13I DdeI DpnII EaeI Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MslI MwoI NdeII NlaIII NlaIV NspI PstI RsaI Sau3AI Sau96I ScrFI SfcI TfiI Tru9I Tsp45I Tsp509I TspRI XmnI