(Linear) (Six-base) MAPSORT of: KIEE9542.seq Check: 1984 from: 1 to: 2271 KIEE9542.orf With 182 enzymes: SgfI * March 22, 2012 18:30 .. Acc65I G'GTAC_C Cuts at: 0 1975 2271 Size: 1975 296 AclI AA'CG_TT Cuts at: 0 1884 2271 Size: 1884 387 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 382 439 586 973 1942 2271 Size: 382 57 147 387 969 329 Fragments arranged by size: 969 387 382 329 147 57 AflIII A'CryG_T Cuts at: 0 1152 2271 Size: 1152 1119 AhdI GACnn_n'nnGTC Cuts at: 0 70 2271 Size: 70 2201 AleI CACnn'nnGTG Cuts at: 0 399 2271 Size: 399 1872 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1272 2271 Size: 1272 999 AlwNI CAG_nnn'CTG Cuts at: 0 132 462 888 1840 2271 Size: 132 330 426 952 431 Fragments arranged by size: 952 431 426 330 132 ApoI r'AATT_y Cuts at: 0 635 663 789 998 1061 1126 1184 1651 Size: 635 28 126 209 63 65 58 467 Cuts at: 1651 1942 2271 Size: 291 329 Fragments arranged by size: 635 467 329 291 209 126 65 63 58 28 AseI AT'TA_AT Cuts at: 0 848 1760 2271 Size: 848 912 511 Fragments arranged by size: 912 848 511 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1475 1508 2271 Size: 1475 33 763 Fragments arranged by size: 1475 763 33 BanI G'GyrC_C Cuts at: 0 1672 1975 2271 Size: 1672 303 296 Fragments arranged by size: 1672 303 296 BanII G_rGCy'C Cuts at: 0 1728 2271 Size: 1728 543 BbsI GAAGACnn'nnnn_ Cuts at: 0 9 2271 Size: 9 2262 BbvCI CC'TCA_GC Cuts at: 0 125 2271 Size: 125 2146 BciVI GTATCCnnnnn_n' Cuts at: 0 1916 2271 Size: 1916 355 BfrBI ATG'CAT Cuts at: 0 1912 2271 Size: 1912 359 BglII A'GATC_T Cuts at: 0 585 2271 Size: 585 1686 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 346 865 2271 Size: 346 519 1406 Fragments arranged by size: 1406 519 346 Bpu10I CC'TnA_GC Cuts at: 0 125 2271 Size: 125 2146 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1018 2271 Size: 1018 1253 BsaI GGTCTCn'nnnn_ Cuts at: 0 209 1557 2271 Size: 209 1348 714 Fragments arranged by size: 1348 714 209 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2124 2154 2271 Size: 2124 30 117 Fragments arranged by size: 2124 117 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 118 2271 Size: 118 2153 BsiHKAI G_wGCw'C Cuts at: 0 82 2271 Size: 82 2189 Bsp1286I G_dGCh'C Cuts at: 0 82 1728 2271 Size: 82 1646 543 Fragments arranged by size: 1646 543 82 BspHI T'CATG_A Cuts at: 0 447 2271 Size: 447 1824 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 476 1264 2271 Size: 476 788 1007 Fragments arranged by size: 1007 788 476 BsrDI GCAATG_nn' Cuts at: 0 313 395 2271 Size: 313 82 1876 Fragments arranged by size: 1876 313 82 BstAPI GCAn_nnn'nTGC Cuts at: 0 2181 2271 Size: 2181 90 BstXI CCAn_nnnn'nTGG Cuts at: 0 1138 2271 Size: 1138 1133 BstYI r'GATC_y Cuts at: 0 585 1465 2271 Size: 585 880 806 Fragments arranged by size: 880 806 585 BtsI GCAGTG_nn' Cuts at: 0 1951 2170 2271 Size: 1951 219 101 Fragments arranged by size: 1951 219 101 DraI TTT'AAA Cuts at: 0 2064 2271 Size: 2064 207 DraIII CAC_nnn'GTG Cuts at: 0 116 2271 Size: 116 2155 DrdI GACnn_nn'nnGTC Cuts at: 0 390 2271 Size: 390 1881 EaeI y'GGCC_r Cuts at: 0 1472 1766 2167 2271 Size: 1472 294 401 104 Fragments arranged by size: 1472 401 294 104 EarI CTCTTCn'nnn_ Cuts at: 0 1700 2271 Size: 1700 571 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 346 382 439 586 865 973 1942 2271 Size: 346 36 57 147 279 108 969 329 Fragments arranged by size: 969 346 329 279 147 108 57 36 EcoO109I rG'GnC_Cy Cuts at: 0 948 2271 Size: 948 1323 FspI TGC'GCA Cuts at: 0 1427 2271 Size: 1427 844 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 56 88 909 941 1217 1249 1280 1312 Size: 56 32 821 32 276 32 31 32 Cuts at: 1312 2271 Size: 959 Fragments arranged by size: 959 821 276 56 32 32 32 32 31 HincII GTy'rAC Cuts at: 0 730 1508 2271 Size: 730 778 763 Fragments arranged by size: 778 763 730 HindIII A'AGCT_T Cuts at: 0 418 2271 Size: 418 1853 HpaI GTT'AAC Cuts at: 0 1508 2271 Size: 1508 763 KpnI G_GTAC'C Cuts at: 0 1979 2271 Size: 1979 292 MfeI C'AATT_G Cuts at: 0 967 2271 Size: 967 1304 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1098 2271 Size: 1098 1173 MscI TGG'CCA Cuts at: 0 1474 2169 2271 Size: 1474 695 102 Fragments arranged by size: 1474 695 102 MslI CAynn'nnrTG Cuts at: 0 59 279 399 857 1282 1823 2155 2271 Size: 59 220 120 458 425 541 332 116 Fragments arranged by size: 541 458 425 332 220 120 116 59 MspA1I CmG'CkG Cuts at: 0 1163 1431 2271 Size: 1163 268 840 Fragments arranged by size: 1163 840 268 NdeI CA'TA_TG Cuts at: 0 2136 2271 Size: 2136 135 NsiI A_TGCA'T Cuts at: 0 1914 2271 Size: 1914 357 NspI r_CATG'y Cuts at: 0 738 1156 1287 1426 1806 2271 Size: 738 418 131 139 380 465 Fragments arranged by size: 738 465 418 380 139 131 PciI A'CATG_T Cuts at: 0 1152 2271 Size: 1152 1119 PflMI CCAn_nnn'nTGG Cuts at: 0 324 888 2271 Size: 324 564 1383 Fragments arranged by size: 1383 564 324 PpuMI rG'GwC_Cy Cuts at: 0 948 2271 Size: 948 1323 PstI C_TGCA'G Cuts at: 0 811 1251 2177 2271 Size: 811 440 926 94 Fragments arranged by size: 926 811 440 94 PvuII CAG'CTG Cuts at: 0 1163 1431 2271 Size: 1163 268 840 Fragments arranged by size: 1163 840 268 ScaI AGT'ACT Cuts at: 0 1135 1446 2271 Size: 1135 311 825 Fragments arranged by size: 1135 825 311 SexAI A'CCwGG_T Cuts at: 0 1971 2271 Size: 1971 300 SfcI C'TryA_G Cuts at: 0 807 1247 2094 2173 2271 Size: 807 440 847 79 98 Fragments arranged by size: 847 807 440 98 79 SmlI C'TyrA_G Cuts at: 0 1033 2271 Size: 1033 1238 SphI G_CATG'C Cuts at: 0 1287 1426 2271 Size: 1287 139 845 Fragments arranged by size: 1287 845 139 SspI AAT'ATT Cuts at: 0 46 223 2271 Size: 46 177 2048 Fragments arranged by size: 2048 177 46 StyI C'CwwG_G Cuts at: 0 84 318 2271 Size: 84 234 1953 Fragments arranged by size: 1953 234 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2145 2271 Size: 2145 126 TatI w'GTAC_w Cuts at: 0 1133 1444 2271 Size: 1133 311 827 Fragments arranged by size: 1133 827 311 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 61 93 2271 Size: 61 32 2178 Fragments arranged by size: 2178 61 32 XbaI T'CTAG_A Cuts at: 0 1194 2271 Size: 1194 1077 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 746 2150 2271 Size: 746 1404 121 Fragments arranged by size: 1404 746 121 XmnI GAAnn'nnTTC Cuts at: 0 587 926 2072 2271 Size: 587 339 1146 199 Fragments arranged by size: 1146 587 339 199 Enzymes that do cut: Acc65I AclI AcuI AflIII AhdI AleI AlfI AlwNI ApoI AseI BaeI BanI BanII BbsI BbvCI BciVI BfrBI BglII BpmI Bpu10I BpuEI BsaI BsaXI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI BstAPI BstXI BstYI BtsI DraI DraIII DrdI EaeI EarI Eco57MI EcoO109I FspI Hin4I HincII HindIII HpaI KpnI MfeI MmeI MscI MslI MspA1I NdeI NsiI NspI PciI PflMI PpuMI PstI PvuII ScaI SexAI SfcI SmlI SphI SspI StyI TaqII TatI TstI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII AccI AfeI AflII AgeI AloI ApaI ApaLI AscI AsiSI AvaI AvrII BamHI BbeI BcgI BclI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI HaeII KasI MluI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PasI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI Tth111I XhoI XmaI ZraI