(Circular) (Six-base) MAPSORT of: pFN21AE6520.seq Check: 6262 from: 1 to: 4867 pFN21AE6520 4867 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2677 278 Size: 53 83 186 936 1141 2468 Fragments arranged by size: 2468 1141 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2210 1937 Size: 273 4594 Acc65I G'GTAC_C Cuts at: 2188 2188 Size: 4867 AclI AA'CG_TT Cuts at: 1563 2994 3367 1563 Size: 1431 373 3063 Fragments arranged by size: 3063 1431 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2930 4314 4582 1632 Size: 1298 1384 268 1917 Fragments arranged by size: 1917 1384 1298 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4670 AflIII A'CryG_T Cuts at: 1203 3766 4743 1203 Size: 2563 977 1327 Fragments arranged by size: 2563 1327 977 AgeI A'CCGG_T Cuts at: 3698 3698 Size: 4867 AhdI GACnn_n'nnGTC Cuts at: 3596 3596 Size: 4867 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4867 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4867 AlwNI CAG_nnn'CTG Cuts at: 2610 4182 2610 Size: 1572 3295 ApaLI G'TGCA_C Cuts at: 2924 4080 2924 Size: 1156 3711 ApoI r'AATT_y Cuts at: 1493 2176 2402 2554 4663 1493 Size: 683 226 152 2109 1697 Fragments arranged by size: 2109 1697 683 226 152 AseI AT'TA_AT Cuts at: 160 1032 3421 160 Size: 872 2389 1606 Fragments arranged by size: 2389 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4867 AvaI C'yCGr_G Cuts at: 1870 1943 2192 1870 Size: 73 249 4545 Fragments arranged by size: 4545 249 73 BamHI G'GATC_C Cuts at: 2034 2197 2564 2034 Size: 163 367 4337 Fragments arranged by size: 4337 367 163 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2188 3643 618 Size: 325 217 627 401 1455 1842 Fragments arranged by size: 1842 1455 627 401 325 217 BanII G_rGCy'C Cuts at: 1391 2186 1391 Size: 795 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 3896 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3058 3092 1903 Size: 34 1121 34 3678 Fragments arranged by size: 3678 1121 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2759 3969 2759 Size: 1210 3657 BclI T'GATC_A Cuts at: 1548 1548 Size: 4867 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3478 136 Size: 107 122 71 631 605 1806 1525 Fragments arranged by size: 1806 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4862 4862 Size: 4867 BlpI GC'TnA_GC Cuts at: 2284 2284 Size: 4867 Bme1580I G_kGCm'C Cuts at: 1790 2928 4084 1790 Size: 1138 1156 2573 Fragments arranged by size: 2573 1156 1138 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4867 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2598 3556 442 Size: 416 53 83 399 24 1181 958 1753 Fragments arranged by size: 1753 1181 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4867 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3527 1239 Size: 2288 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2992 3857 4155 2992 Size: 865 298 3704 Fragments arranged by size: 3704 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3530 882 Size: 617 2031 2219 Fragments arranged by size: 2219 2031 617 BsaAI yAC'GTr Cuts at: 493 1204 2658 493 Size: 711 1454 2702 Fragments arranged by size: 2702 1454 711 BsaBI GATnn'nnATC Cuts at: 4861 4861 Size: 4867 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2674 3056 3691 275 Size: 53 83 186 936 1141 382 635 1451 Fragments arranged by size: 1451 1141 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3300 3698 3972 4119 4530 3300 Size: 398 274 147 411 3637 Fragments arranged by size: 3637 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4642 Fragments arranged by size: 4642 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4867 BseYI C'CCAG_C Cuts at: 4070 4566 4070 Size: 496 4371 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4294 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2317 3078 4106 1540 Size: 275 186 316 761 1028 2301 Fragments arranged by size: 2301 1028 761 316 275 186 BsiHKAI G_wGCw'C Cuts at: 2928 3013 4084 2928 Size: 85 1071 3711 Fragments arranged by size: 3711 1071 85 BsmI GAATG_Cn' Cuts at: 2378 2471 2378 Size: 93 4774 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2186 2928 3013 4084 1391 Size: 399 396 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 396 85 BspEI T'CCGG_A Cuts at: 4530 4530 Size: 4867 BspHI T'CATG_A Cuts at: 2756 4486 2756 Size: 1730 3137 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2222 844 Size: 1378 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2754 1394 Size: 429 931 3507 Fragments arranged by size: 3507 931 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3362 3536 65 Size: 1183 2114 174 1396 Fragments arranged by size: 2114 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3511 3698 1700 Size: 110 1701 187 2869 Fragments arranged by size: 2869 1701 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4867 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4867 BssSI C'ACGA_G Cuts at: 2927 3939 2927 Size: 1012 3855 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4867 BstBI TT'CG_AA Cuts at: 3671 3671 Size: 4867 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4867 BstYI r'GATC_y Cuts at: 1972 2034 2083 2197 2564 2949 2966 4418 4504 Size: 62 49 114 367 385 17 1452 86 Cuts at: 4504 4516 4862 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 114 86 62 49 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3376 Fragments arranged by size: 3376 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4128 Fragments arranged by size: 4128 683 56 BtsI GCAGTG_nn' Cuts at: 2383 3177 3197 2383 Size: 794 20 4053 Fragments arranged by size: 4053 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4867 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4832 DraI TTT'AAA Cuts at: 2171 2171 Size: 4867 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3874 809 Size: 507 2558 1802 Fragments arranged by size: 2558 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2143 2314 3203 Size: 54 1425 50 275 18 313 171 889 Cuts at: 3203 4832 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 313 275 171 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2314 1537 Size: 275 502 4090 Fragments arranged by size: 4090 502 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2797 1353 Size: 1444 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3454 3828 3974 387 Size: 1064 138 1865 374 146 1280 Fragments arranged by size: 1865 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2930 3527 4314 4582 1239 Size: 393 1298 597 787 268 1524 Fragments arranged by size: 1524 1298 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2029 2029 Size: 4867 EcoRI G'AATT_C Cuts at: 2176 2554 2176 Size: 378 4489 FspI TGC'GCA Cuts at: 3373 3373 Size: 4867 HaeII r_GCGC'y Cuts at: 1678 4014 1678 Size: 2336 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2026 2058 3508 3540 3582 Size: 32 135 32 454 32 1450 32 42 Cuts at: 3582 3614 1373 Size: 32 2626 Fragments arranged by size: 2626 1450 454 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2211 2457 669 Size: 988 281 273 246 3079 Fragments arranged by size: 3079 988 281 273 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4867 HpaI GTT'AAC Cuts at: 2457 2457 Size: 4867 KpnI G_GTAC'C Cuts at: 2192 2192 Size: 4867 MfeI C'AATT_G Cuts at: 2466 2466 Size: 4867 MluI A'CGCG_T Cuts at: 4743 4743 Size: 4867 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3981 4165 1305 Size: 2676 184 2007 Fragments arranged by size: 2676 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2145 10 Size: 54 1425 343 313 2732 Fragments arranged by size: 2732 1425 343 313 54 MslI CAynn'nnrTG Cuts at: 518 1166 2825 3184 3343 518 Size: 648 1659 359 159 2042 Fragments arranged by size: 2042 1659 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2119 2283 2960 4108 4353 1606 Size: 513 164 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 513 245 164 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3376 Fragments arranged by size: 3376 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 4867 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4867 NotI GC'GGCC_GC Cuts at: 2314 2314 Size: 4867 NspI r_CATG'y Cuts at: 1132 1229 2225 3770 1132 Size: 97 996 1545 2229 Fragments arranged by size: 2229 1545 996 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4867 PciI A'CATG_T Cuts at: 3766 3766 Size: 4867 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4867 PmeI GTTT'AAAC Cuts at: 2171 2171 Size: 4867 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2936 2968 4449 4481 2936 Size: 32 1481 32 3322 Fragments arranged by size: 3322 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4867 PsiI TTA'TAA Cuts at: 2437 2437 Size: 4867 PstI C_TGCA'G Cuts at: 830 2219 830 Size: 1389 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4867 PvuII CAG'CTG Cuts at: 2119 2119 Size: 4867 SalI G'TCGA_C Cuts at: 1936 2209 1936 Size: 273 4594 SbfI CC_TGCA'GG Cuts at: 2219 2219 Size: 4867 ScaI AGT'ACT Cuts at: 3115 4827 3115 Size: 1712 3155 SfcI C'TryA_G Cuts at: 826 1045 2215 3350 4031 4222 826 Size: 219 1170 1135 681 191 1471 Fragments arranged by size: 1471 1170 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4867 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4867 SmaI CCC'GGG Cuts at: 1872 2194 1872 Size: 322 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2971 3872 4134 820 Size: 197 926 1028 901 262 1553 Fragments arranged by size: 1553 1028 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2658 493 Size: 2165 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4867 SphI G_CATG'C Cuts at: 1132 2225 1132 Size: 1093 3774 SspI AAT'ATT Cuts at: 5 52 2791 5 Size: 47 2739 2081 Fragments arranged by size: 2739 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4867 StyI C'CwwG_G Cuts at: 513 1065 2004 2306 513 Size: 552 939 302 3074 Fragments arranged by size: 3074 939 552 302 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2894 2911 3064 3249 3694 325 Size: 1255 1314 17 153 185 445 1498 Fragments arranged by size: 1498 1314 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3113 4825 96 Size: 274 80 33 51 1443 1136 1712 138 Fragments arranged by size: 1712 1443 1136 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4827 4859 4827 Size: 32 4835 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4867 XbaI T'CTAG_A Cuts at: 2203 2203 Size: 4867 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2138 2011 Size: 127 4740 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4867 XmaI C'CCGG_G Cuts at: 1870 2192 1870 Size: 322 4545 XmnI GAAnn'nnTTC Cuts at: 2996 2996 Size: 4867 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2675 276 Size: 53 83 186 936 1141 2468 Fragments arranged by size: 2468 1141 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI