(Circular) (Six-base) MAPSORT of: pFN21AE3992.seq Check: 6847 from: 1 to: 5860 pFN21AE3992 5860 bp With 182 enzymes: SgfI * March 28, 2012 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3670 278 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3203 1937 Size: 1266 4594 Acc65I G'GTAC_C Cuts at: 2355 3181 2355 Size: 826 5034 AclI AA'CG_TT Cuts at: 1563 3987 4360 1563 Size: 2424 373 3063 Fragments arranged by size: 3063 2424 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2197 2963 3923 5307 5575 1632 Size: 565 766 960 1384 268 1917 Fragments arranged by size: 1917 1384 960 766 565 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5663 AflIII A'CryG_T Cuts at: 1203 4759 5736 1203 Size: 3556 977 1327 Fragments arranged by size: 3556 1327 977 AgeI A'CCGG_T Cuts at: 4691 4691 Size: 5860 AhdI GACnn_n'nnGTC Cuts at: 4589 4589 Size: 5860 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5860 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5860 AlwNI CAG_nnn'CTG Cuts at: 3002 3603 5175 3002 Size: 601 1572 3687 Fragments arranged by size: 3687 1572 601 ApaLI G'TGCA_C Cuts at: 3917 5073 3917 Size: 1156 4704 ApoI r'AATT_y Cuts at: 1493 2541 2558 2583 2675 2819 3169 3395 3547 Size: 1048 17 25 92 144 350 226 152 Cuts at: 3547 5656 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1048 350 226 152 144 92 25 17 AseI AT'TA_AT Cuts at: 160 1032 4414 160 Size: 872 3382 1606 Fragments arranged by size: 3382 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 AvaI C'yCGr_G Cuts at: 1870 1943 3185 1870 Size: 73 1242 4545 Fragments arranged by size: 4545 1242 73 BamHI G'GATC_C Cuts at: 3190 3557 3190 Size: 367 5493 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2355 2665 3181 4636 618 Size: 325 217 627 568 310 516 1455 1842 Fragments arranged by size: 1842 1455 627 568 516 325 310 217 BanII G_rGCy'C Cuts at: 1391 3179 1391 Size: 1788 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2203 3036 928 Size: 971 304 833 3752 Fragments arranged by size: 3752 971 833 304 BbvCI CC'TCA_GC Cuts at: 3051 3051 Size: 5860 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4051 4085 1903 Size: 34 2114 34 3678 Fragments arranged by size: 3678 2114 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3752 4962 3752 Size: 1210 4650 BclI T'GATC_A Cuts at: 1548 1548 Size: 5860 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4471 136 Size: 107 122 71 631 605 2799 1525 Fragments arranged by size: 2799 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5855 5855 Size: 5860 BlpI GC'TnA_GC Cuts at: 3277 3277 Size: 5860 Bme1580I G_kGCm'C Cuts at: 1790 3921 5077 1790 Size: 2131 1156 2573 Fragments arranged by size: 2573 2131 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5860 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3591 4549 442 Size: 416 53 83 399 24 2174 958 1753 Fragments arranged by size: 2174 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5860 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2620 2620 Size: 5860 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3007 4520 1239 Size: 1768 1513 2579 Fragments arranged by size: 2579 1768 1513 Bpu10I CC'TnA_GC Cuts at: 3051 3051 Size: 5860 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2223 3985 4850 5148 2223 Size: 1762 865 298 2935 Fragments arranged by size: 2935 1762 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2003 4523 882 Size: 617 504 2520 2219 Fragments arranged by size: 2520 2219 617 504 BsaAI yAC'GTr Cuts at: 493 1204 3651 493 Size: 711 2447 2702 Fragments arranged by size: 2702 2447 711 BsaBI GATnn'nnATC Cuts at: 5854 5854 Size: 5860 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3667 4049 4684 275 Size: 53 83 186 936 2134 382 635 1451 Fragments arranged by size: 2134 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4293 4691 4965 5112 5523 4293 Size: 398 274 147 411 4630 Fragments arranged by size: 4630 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5635 Fragments arranged by size: 5635 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2403 1186 Size: 1217 4643 BseYI C'CCAG_C Cuts at: 2078 2373 5063 5559 2078 Size: 295 2690 496 2379 Fragments arranged by size: 2690 2379 496 295 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5287 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3310 4071 5099 1540 Size: 275 186 1309 761 1028 2301 Fragments arranged by size: 2301 1309 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3921 4006 5077 3921 Size: 85 1071 4704 Fragments arranged by size: 4704 1071 85 BsmI GAATG_Cn' Cuts at: 3371 3464 3371 Size: 93 5767 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3179 3921 4006 5077 1391 Size: 399 1389 742 85 1071 2174 Fragments arranged by size: 2174 1389 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5523 5523 Size: 5860 BspHI T'CATG_A Cuts at: 2416 3749 5479 2416 Size: 1333 1730 2797 Fragments arranged by size: 2797 1730 1333 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3215 844 Size: 2371 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3747 1394 Size: 429 1924 3507 Fragments arranged by size: 3507 1924 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3079 4355 4529 65 Size: 1183 1831 1276 174 1396 Fragments arranged by size: 1831 1396 1276 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2782 4504 4691 1700 Size: 110 972 1722 187 2869 Fragments arranged by size: 2869 1722 972 187 110 BsrGI T'GTAC_A Cuts at: 96 2526 96 Size: 2430 3430 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5860 BssSI C'ACGA_G Cuts at: 3920 4932 3920 Size: 1012 4848 BstAPI GCAn_nnn'nTGC Cuts at: 794 2890 794 Size: 2096 3764 BstBI TT'CG_AA Cuts at: 2500 4664 2500 Size: 2164 3696 BstEII G'GTnAC_C Cuts at: 1184 2861 1184 Size: 1677 4183 BstXI CCAn_nnnn'nTGG Cuts at: 2020 2020 Size: 5860 BstYI r'GATC_y Cuts at: 1972 3190 3557 3942 3959 5411 5497 5509 5855 Size: 1218 367 385 17 1452 86 12 346 Cuts at: 5855 1972 Size: 1977 Fragments arranged by size: 1977 1452 1218 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2969 3063 513 Size: 552 939 965 94 3310 Fragments arranged by size: 3310 965 939 552 94 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5121 Fragments arranged by size: 5121 683 56 BtsI GCAGTG_nn' Cuts at: 2888 3376 4170 4190 2888 Size: 488 794 20 4558 Fragments arranged by size: 4558 794 488 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5860 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5825 DraI TTT'AAA Cuts at: 2937 3164 2937 Size: 227 5633 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4867 809 Size: 507 3551 1802 Fragments arranged by size: 3551 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2685 3307 4196 Size: 54 1425 50 275 18 855 622 889 Cuts at: 4196 5825 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 855 622 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3307 1537 Size: 275 1495 4090 Fragments arranged by size: 4090 1495 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2572 3790 1353 Size: 1219 1218 3423 Fragments arranged by size: 3423 1219 1218 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2813 4447 4821 4967 387 Size: 1064 138 1224 1634 374 146 1280 Fragments arranged by size: 1634 1280 1224 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2197 2963 3007 3923 4520 5307 5575 Size: 393 565 766 44 916 597 787 268 Cuts at: 5575 1239 Size: 1524 Fragments arranged by size: 1524 916 787 766 597 565 393 268 44 EcoRI G'AATT_C Cuts at: 2541 3169 3547 2541 Size: 628 378 4854 Fragments arranged by size: 4854 628 378 EcoRV GAT'ATC Cuts at: 2461 2461 Size: 5860 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3109 3109 Size: 5860 FspI TGC'GCA Cuts at: 4366 4366 Size: 5860 HaeII r_GCGC'y Cuts at: 1678 5007 1678 Size: 3329 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4501 4533 4575 4607 1373 Size: 32 135 32 2929 32 42 32 2626 Fragments arranged by size: 2929 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3204 3450 669 Size: 988 281 1266 246 3079 Fragments arranged by size: 3079 1266 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5860 HpaI GTT'AAC Cuts at: 3450 3450 Size: 5860 KpnI G_GTAC'C Cuts at: 2359 3185 2359 Size: 826 5034 MfeI C'AATT_G Cuts at: 3459 3459 Size: 5860 MluI A'CGCG_T Cuts at: 5736 5736 Size: 5860 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4974 5158 1305 Size: 3669 184 2007 Fragments arranged by size: 3669 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2687 10 Size: 54 1425 343 855 3183 Fragments arranged by size: 3183 1425 855 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2421 2631 3818 4177 4336 518 Size: 648 1255 210 1187 359 159 2042 Fragments arranged by size: 2042 1255 1187 648 359 210 159 MspA1I CmG'CkG Cuts at: 1606 3276 3953 5101 5346 1606 Size: 1670 677 1148 245 2120 Fragments arranged by size: 2120 1670 1148 677 245 NaeI GCC'GGC Cuts at: 2784 2784 Size: 5860 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4369 Fragments arranged by size: 4369 939 552 NdeI CA'TA_TG Cuts at: 387 2302 387 Size: 1915 3945 NgoMIV G'CCGG_C Cuts at: 2782 2782 Size: 5860 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5860 NotI GC'GGCC_GC Cuts at: 3307 3307 Size: 5860 NspI r_CATG'y Cuts at: 1132 1229 2158 3050 3072 3218 4763 1132 Size: 97 929 892 22 146 1545 2229 Fragments arranged by size: 2229 1545 929 892 146 97 22 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5860 PciI A'CATG_T Cuts at: 4759 4759 Size: 5860 PflMI CCAn_nnn'nTGG Cuts at: 1108 2303 1108 Size: 1195 4665 PfoI T'CCnGG_A Cuts at: 2040 2536 2040 Size: 496 5364 PmeI GTTT'AAAC Cuts at: 3164 3164 Size: 5860 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3929 3961 5442 5474 3929 Size: 32 1481 32 4315 Fragments arranged by size: 4315 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5860 PsiI TTA'TAA Cuts at: 3430 3430 Size: 5860 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2331 2363 2331 Size: 32 5828 PstI C_TGCA'G Cuts at: 830 2057 2895 3212 830 Size: 1227 838 317 3478 Fragments arranged by size: 3478 1227 838 317 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5860 SalI G'TCGA_C Cuts at: 1936 3202 1936 Size: 1266 4594 SbfI CC_TGCA'GG Cuts at: 3212 3212 Size: 5860 ScaI AGT'ACT Cuts at: 4108 5820 4108 Size: 1712 4148 SfcI C'TryA_G Cuts at: 826 1045 2053 2891 3208 4343 5024 5215 826 Size: 219 1008 838 317 1135 681 191 1471 Fragments arranged by size: 1471 1135 1008 838 681 317 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5860 SmaI CCC'GGG Cuts at: 1872 3187 1872 Size: 1315 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2238 3964 4865 5127 820 Size: 197 926 295 1726 901 262 1553 Fragments arranged by size: 1726 1553 926 901 295 262 197 SnaBI TAC'GTA Cuts at: 493 3651 493 Size: 3158 2702 SpeI A'CTAG_T Cuts at: 152 2344 152 Size: 2192 3668 SphI G_CATG'C Cuts at: 1132 3218 1132 Size: 2086 3774 SspI AAT'ATT Cuts at: 5 52 3784 5 Size: 47 3732 2081 Fragments arranged by size: 3732 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5860 StyI C'CwwG_G Cuts at: 513 1065 2004 3299 513 Size: 552 939 1295 3074 Fragments arranged by size: 3074 1295 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3887 3904 4057 4242 4687 325 Size: 1255 2307 17 153 185 445 1498 Fragments arranged by size: 2307 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2338 2526 4106 Size: 274 80 33 51 1443 361 188 1580 Cuts at: 4106 5818 96 Size: 1712 138 Fragments arranged by size: 1712 1580 1443 361 274 188 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3048 3080 5820 5852 3048 Size: 32 2740 32 3056 Fragments arranged by size: 3056 2740 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5860 XbaI T'CTAG_A Cuts at: 3196 3196 Size: 5860 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2659 2712 2659 Size: 53 5807 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5860 XmaI C'CCGG_G Cuts at: 1870 3185 1870 Size: 1315 4545 XmnI GAAnn'nnTTC Cuts at: 3989 3989 Size: 5860 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3668 276 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI