################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstUI BstYI BstZ17I CelII CfoI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MflI MluI MroI MseI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tth111I XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AfaI 518 GTAC GT^AC Alw44I 407 GTGCAC G^TGCAC ApaLI 407 GTGCAC G^TGCAC BanI 178 GGCACC G^G[CT][GA]CC BbvCI 119 CCTCAGC CCTCAGC(-5/-2) BglI 975 GCCCACGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BspDI 301 ATCGAT AT^CGAT BsrFI 984 GCCGGT [GA]^CCGG[CT] BstAPI 400 GCAGTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 684 GGTGACC G^GT[ACGT]ACC BstPI 684 GGTGACC G^GT[ACGT]ACC BstXI 362 CCAGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 984 GCCGGT [GA]^CCGG[CT] ClaI 301 ATCGAT AT^CGAT EcoO65I 684 GGTGACC G^GT[ACGT]ACC EcoT22I 1 ATGCAT ATGCA^T FauI 395 GCGGG CCCGC(4/6) HinfI 227 GAGTC G^A[ACGT]TC NsiI 1 ATGCAT ATGCA^T NspI 478 GCATGT [GA]CATG^[CT] PflMI 341 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 227 GAGTC GAGTC(4/5) PstI 43 CTGCAG CTGCA^G RsaI 518 GTAC GT^AC SfcI 43 CTGCAG C^T[GA][CT]AG SfiI 974 GGCCCACGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC StuI 644 AGGCCT AGG^CCT TaqI 302 TCGA T^CGA Tsp509I 453 AATT ^AATT TthHB8I 302 TCGA T^CGA Van91I 341 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 262 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AciI 373 GCGG CCGC(-3/-1) 395 GCGG AlwNI 34 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG 342 CAGCCCCTG AocI 162 CCTGAGG CC^T[ACGT]AGG 426 CCTGAGG ApaI 115 GGGCCC GGGCC^C 973 GGGCCC AspHI 407 GTGCAC G[AT]GC[AT]^C 435 GTGCTC BcnI 512 CCCGG CC^[GC]GG 526 CCCGG BfaI 106 CTAG C^TAG 263 CTAG BsgI 92 CTGCAC GTGCAG(16/14) 207 CTGCAC BsiHKAI 407 GTGCAC G[AT]GC[AT]^C 435 GTGCTC BsmFI 330 GGGAC GGGAC(10/14) 129 GTCCC BssSI 534 CACGAG CACGAG(-5/-1) 438 CTCGTG Bsu36I 162 CCTGAGG CC^T[ACGT]AGG 426 CCTGAGG DsaI 57 CCACGG C^C[GA][CT]GG 977 CCACGG EarI 355 CTCTTC CTCTTC(1/4) 640 GAAGAG Eco81I 162 CCTGAGG CC^T[ACGT]AGG 426 CCTGAGG Ksp632I 355 CTCTTC CTCTTC(1/4) 640 GAAGAG MaeI 106 CTAG C^TAG 263 CTAG MboII 640 GAAGA GAAGA(8/7) 356 TCTTC MslI 379 CACATAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 410 CACCGTCGTG NciI 512 CCCGG CC^[GC]GG 526 CCCGG Tsp45I 235 GTCAC ^GT[GC]AC 685 GTGAC XcmI 473 CCACTGCATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 868 CCACCCTGGGGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AluI AlwI AvaII BalI BanII BbvI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeIII MboI MluNI MnlI MscI MspI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI StyI TseI TspRI