################################## # NO CUT ENZYMES ( 181 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BciVI BclI BfrI BglI BglII BlnI BmyI BpmI BpuAI BsaBI BsaHI BsaJI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstXI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DraIII DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HaeIII Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI MvaI NaeI NarI NcoI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TaqI TfiI TthHB8I XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcsI 464 GAATTC [GA]^AATT[CT] AfaI 451 GTAC GT^AC AflIII 492 ACACGT A^C[GA][CT]GT AluI 350 AGCT AG^CT ApoI 464 GAATTC [GA]^AATT[CT] AspI 432 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 166 GGACC G^G[AT]CC BcgI 347 CGAAGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 111 CCCGG CC^[GC]GG BlpI 45 GCTGAGC GC^T[ACGT]AGC Bpu1102I 45 GCTGAGC GC^T[ACGT]AGC BsaAI 493 CACGTA [CT]AC^GT[GA] BsaI 403 GGTCTC GGTCTC(1/5) BsgI 121 CTGCAC GTGCAG(16/14) BsmBI 126 CGTCTC CGTCTC(1/5) BsrI 163 ACTGG ACTGG(1/-1) BssKI 111 CCCGG ^CC[ACGT]GG Cac8I 18 GCGGGC GC[ACGT]^[ACGT]GC CelII 45 GCTGAGC GC^T[ACGT]AGC CfoI 61 GCGC GCG^C Cfr13I 166 GGACC G^G[ACGT]CC DdeI 46 CTGAG C^T[ACGT]AG DrdI 100 GACAGGAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 531 GAAGAG CTCTTC(1/4) EcoRI 464 GAATTC G^AATTC FauI 18 GCGGG CCCGC(4/6) HaeII 60 GGCGCT [GA]GCGC^[CT] HapII 112 CCGG C^CGG HgaI 107 GCGTC GACGC(5/10) HhaI 61 GCGC GCG^C HinP1I 61 GCGC G^CGC HpaII 112 CCGG C^CGG Ksp632I 531 GAAGAG CTCTTC(1/4) MseI 245 TTAA T^TAA MspI 112 CCGG C^CGG NciI 111 CCCGG CC^[GC]GG NdeI 518 CATATG CA^TATG NlaIII 138 CATG CATG^ PflFI 432 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 454 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 451 GTAC GT^AC Sau96I 166 GGACC G^G[ACGT]CC ScrFI 111 CCCGG CC^[ACGT]GG SmlI 406 CTCAAG C^T[CT][GA]AG Tru9I 245 TTAA T^TAA Tsp509I 465 AATT ^AATT Tth111I 432 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 454 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 219 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AccII 17 CGCG CG^CG 131 CGCG AlwI 134 GGATC GGATC(4/5) 484 GGATC BfaI 170 CTAG C^TAG 220 CTAG BsiYI 434 CCCTGTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 454 CCAAATGCTGG BslI 434 CCCTGTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 454 CCAAATGCTGG BsmAI 127 GTCTC GTCTC(1/5) 404 GTCTC BstUI 17 CGCG CG^CG 131 CGCG MaeI 170 CTAG C^TAG 220 CTAG MaeIII 424 GTCAC ^GT[ACGT]AC 478 GTGAC MboII 154 GAAGA GAAGA(8/7) 531 GAAGA MnlI 472 CCTC CCTC(7/6) 335 GAGG MvnI 17 CGCG CG^CG 131 CGCG MwoI 123 GCACGTCTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 132 GCGGATCATGC SspI 275 AATATT AAT^ATT 377 AATATT Tsp45I 424 GTCAC ^GT[GC]AC 478 GTGAC TspRI 29 CACTG CA[GC]TG[ACGT][ACGT]^ 162 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### AciI BbvI BsmFI DpnII Fnu4HI HphI ItaI MaeII MboI NdeII Sau3AI TseI