(Circular) (Six-base) MAPSORT of: pFN21AE5251.seq Check: 2575 from: 1 to: 5245 pFN21AE5251 5245 bp With 182 enzymes: SgfI * January 30, 2014 17:15 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3055 278 Size: 53 83 186 936 1519 2468 Fragments arranged by size: 2468 1519 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2588 1937 Size: 651 4594 Acc65I G'GTAC_C Cuts at: 2566 2566 Size: 5245 AclI AA'CG_TT Cuts at: 1563 3372 3745 1563 Size: 1809 373 3063 Fragments arranged by size: 3063 1809 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3308 4692 4960 1632 Size: 1676 1384 268 1917 Fragments arranged by size: 1917 1676 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5048 AflIII A'CryG_T Cuts at: 1203 2497 4144 5121 1203 Size: 1294 1647 977 1327 Fragments arranged by size: 1647 1327 1294 977 AgeI A'CCGG_T Cuts at: 4076 4076 Size: 5245 AhdI GACnn_n'nnGTC Cuts at: 3974 3974 Size: 5245 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5245 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5245 AlwNI CAG_nnn'CTG Cuts at: 2988 4560 2988 Size: 1572 3673 ApaLI G'TGCA_C Cuts at: 3302 4458 3302 Size: 1156 4089 ApoI r'AATT_y Cuts at: 1493 2469 2554 2780 2932 5041 1493 Size: 976 85 226 152 2109 1697 Fragments arranged by size: 2109 1697 976 226 152 85 AseI AT'TA_AT Cuts at: 160 1032 3799 160 Size: 872 2767 1606 Fragments arranged by size: 2767 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5245 AvaI C'yCGr_G Cuts at: 1870 1943 2570 1870 Size: 73 627 4545 Fragments arranged by size: 4545 627 73 BamHI G'GATC_C Cuts at: 2575 2942 2575 Size: 367 4878 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2566 4021 618 Size: 325 217 627 779 1455 1842 Fragments arranged by size: 1842 1455 779 627 325 217 BanII G_rGCy'C Cuts at: 1391 2564 1391 Size: 1173 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4274 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2341 2375 3436 3470 1903 Size: 34 404 34 1061 34 3678 Fragments arranged by size: 3678 1061 404 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3137 4347 3137 Size: 1210 4035 BclI T'GATC_A Cuts at: 1548 1548 Size: 5245 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3856 136 Size: 107 122 71 631 605 2184 1525 Fragments arranged by size: 2184 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5240 5240 Size: 5245 BlpI GC'TnA_GC Cuts at: 2051 2662 2051 Size: 611 4634 Bme1580I G_kGCm'C Cuts at: 1790 3306 4462 1790 Size: 1516 1156 2573 Fragments arranged by size: 2573 1516 1156 BmgBI CAC'GTC Cuts at: 1318 2131 1318 Size: 813 4432 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2976 3934 442 Size: 416 53 83 399 24 1559 958 1753 Fragments arranged by size: 1753 1559 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5245 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3905 1239 Size: 2666 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2396 3370 4235 4533 2396 Size: 974 865 298 3108 Fragments arranged by size: 3108 974 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2414 3908 882 Size: 617 915 1494 2219 Fragments arranged by size: 2219 1494 915 617 BsaAI yAC'GTr Cuts at: 493 1204 2500 3036 493 Size: 711 1296 536 2702 Fragments arranged by size: 2702 1296 711 536 BsaBI GATnn'nnATC Cuts at: 5239 5239 Size: 5245 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3052 3434 4069 275 Size: 53 83 186 936 1519 382 635 1451 Fragments arranged by size: 1519 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3678 4076 4350 4497 4908 3678 Size: 398 274 147 411 4015 Fragments arranged by size: 4015 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5020 Fragments arranged by size: 5020 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5245 BseYI C'CCAG_C Cuts at: 4448 4944 4448 Size: 496 4749 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2111 1163 Size: 573 375 4297 Fragments arranged by size: 4297 573 375 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2695 3456 4484 1540 Size: 275 186 694 761 1028 2301 Fragments arranged by size: 2301 1028 761 694 275 186 BsiHKAI G_wGCw'C Cuts at: 3306 3391 4462 3306 Size: 85 1071 4089 Fragments arranged by size: 4089 1071 85 BsmI GAATG_Cn' Cuts at: 2756 2849 2756 Size: 93 5152 BsmBI CGTCTCn'nnnn_ Cuts at: 2137 2137 Size: 5245 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2564 3306 3391 4462 1391 Size: 399 774 742 85 1071 2174 Fragments arranged by size: 2174 1071 774 742 399 85 BspEI T'CCGG_A Cuts at: 4908 4908 Size: 5245 BspHI T'CATG_A Cuts at: 3134 4864 3134 Size: 1730 3515 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2600 844 Size: 1756 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3132 1394 Size: 429 1309 3507 Fragments arranged by size: 3507 1309 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3740 3914 65 Size: 1183 2492 174 1396 Fragments arranged by size: 2492 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3889 4076 1700 Size: 110 2079 187 2869 Fragments arranged by size: 2869 2079 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5245 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5245 BssSI C'ACGA_G Cuts at: 3305 4317 3305 Size: 1012 4233 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5245 BstBI TT'CG_AA Cuts at: 4049 4049 Size: 5245 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5245 BstYI r'GATC_y Cuts at: 1972 2575 2942 3327 3344 4796 4882 4894 5240 Size: 603 367 385 17 1452 86 12 346 Cuts at: 5240 1972 Size: 1977 Fragments arranged by size: 1977 1452 603 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4693 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4506 Fragments arranged by size: 4506 683 56 BtsI GCAGTG_nn' Cuts at: 2761 3555 3575 2761 Size: 794 20 4431 Fragments arranged by size: 4431 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5245 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5210 DraI TTT'AAA Cuts at: 2549 2549 Size: 5245 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2111 4252 809 Size: 507 795 2141 1802 Fragments arranged by size: 2141 1802 795 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2692 3581 5210 Size: 54 1425 50 275 18 862 889 1629 Cuts at: 5210 8 Size: 43 Fragments arranged by size: 1629 1425 889 862 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2692 1537 Size: 275 880 4090 Fragments arranged by size: 4090 880 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2531 3175 1353 Size: 1178 644 3423 Fragments arranged by size: 3423 1178 644 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3832 4206 4352 387 Size: 1064 138 2243 374 146 1280 Fragments arranged by size: 2243 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3308 3905 4692 4960 1239 Size: 393 1676 597 787 268 1524 Fragments arranged by size: 1676 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2469 2554 2932 2469 Size: 85 378 4782 Fragments arranged by size: 4782 378 85 FspI TGC'GCA Cuts at: 3751 3751 Size: 5245 HaeII r_GCGC'y Cuts at: 1678 2069 4392 1678 Size: 391 2323 2531 Fragments arranged by size: 2531 2323 391 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3886 3918 3960 3992 1373 Size: 32 135 32 2314 32 42 32 2626 Fragments arranged by size: 2626 2314 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2589 2835 669 Size: 988 281 651 246 3079 Fragments arranged by size: 3079 988 651 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5245 HpaI GTT'AAC Cuts at: 2835 2835 Size: 5245 KpnI G_GTAC'C Cuts at: 2570 2570 Size: 5245 MfeI C'AATT_G Cuts at: 2844 2844 Size: 5245 MluI A'CGCG_T Cuts at: 5121 5121 Size: 5245 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4359 4543 1305 Size: 3054 184 2007 Fragments arranged by size: 3054 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3423 Fragments arranged by size: 3423 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3203 3562 3721 518 Size: 648 2037 359 159 2042 Fragments arranged by size: 2042 2037 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2661 3338 4486 4731 1606 Size: 1055 677 1148 245 2120 Fragments arranged by size: 2120 1148 1055 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4693 NdeI CA'TA_TG Cuts at: 387 2524 387 Size: 2137 3108 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5245 NotI GC'GGCC_GC Cuts at: 2692 2692 Size: 5245 NspI r_CATG'y Cuts at: 1132 1229 2603 4148 1132 Size: 97 1374 1545 2229 Fragments arranged by size: 2229 1545 1374 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5245 PciI A'CATG_T Cuts at: 4144 4144 Size: 5245 PflMI CCAn_nnn'nTGG Cuts at: 1108 2465 1108 Size: 1357 3888 PmeI GTTT'AAAC Cuts at: 2549 2549 Size: 5245 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3314 3346 4827 4859 3314 Size: 32 1481 32 3700 Fragments arranged by size: 3700 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5245 PsiI TTA'TAA Cuts at: 2815 2815 Size: 5245 PstI C_TGCA'G Cuts at: 830 2597 830 Size: 1767 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5245 SalI G'TCGA_C Cuts at: 1936 2587 1936 Size: 651 4594 SbfI CC_TGCA'GG Cuts at: 2597 2597 Size: 5245 ScaI AGT'ACT Cuts at: 3493 5205 3493 Size: 1712 3533 SfcI C'TryA_G Cuts at: 826 1045 2593 3728 4409 4600 826 Size: 219 1548 1135 681 191 1471 Fragments arranged by size: 1548 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5245 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5245 SmaI CCC'GGG Cuts at: 1872 2572 1872 Size: 700 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2411 3349 4250 4512 820 Size: 197 926 468 938 901 262 1553 Fragments arranged by size: 1553 938 926 901 468 262 197 SnaBI TAC'GTA Cuts at: 493 3036 493 Size: 2543 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5245 SphI G_CATG'C Cuts at: 1132 2603 1132 Size: 1471 3774 SspI AAT'ATT Cuts at: 5 52 2282 2384 3169 5 Size: 47 2230 102 785 2081 Fragments arranged by size: 2230 2081 785 102 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5245 StyI C'CwwG_G Cuts at: 513 1065 2684 513 Size: 552 1619 3074 Fragments arranged by size: 3074 1619 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3272 3289 3442 3627 4072 325 Size: 1255 1692 17 153 185 445 1498 Fragments arranged by size: 1692 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3491 5203 96 Size: 274 80 33 51 1443 1514 1712 138 Fragments arranged by size: 1712 1514 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5205 5237 5205 Size: 32 5213 Tth111I GACn'n_nGTC Cuts at: 1316 2440 1316 Size: 1124 4121 XbaI T'CTAG_A Cuts at: 2224 2581 2224 Size: 357 4888 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5245 XmaI C'CCGG_G Cuts at: 1870 2570 1870 Size: 700 4545 XmnI GAAnn'nnTTC Cuts at: 3374 3374 Size: 5245 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3053 276 Size: 53 83 186 936 1519 2468 Fragments arranged by size: 2468 1519 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI