(Linear) (Six-base) MAPSORT of: KIEE2550.seq Check: 5203 from: 1 to: 1149 KIEE2550.orf With 182 enzymes: SgfI * October 31, 2014 16:16 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 850 1149 Size: 850 299 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 892 1149 Size: 892 257 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 820 852 1149 Size: 820 32 297 Fragments arranged by size: 820 297 32 AlwNI CAG_nnn'CTG Cuts at: 0 1044 1107 1149 Size: 1044 63 42 Fragments arranged by size: 1044 63 42 ApaI G_GGCC'C Cuts at: 0 13 1149 Size: 13 1136 ApoI r'AATT_y Cuts at: 0 498 1149 Size: 498 651 BanI G'GyrC_C Cuts at: 0 1144 1149 Size: 1144 5 BanII G_rGCy'C Cuts at: 0 13 74 388 416 474 1028 1149 Size: 13 61 314 28 58 554 121 Fragments arranged by size: 554 314 121 61 58 28 13 BbsI GAAGACnn'nnnn_ Cuts at: 0 9 73 374 529 1149 Size: 9 64 301 155 620 Fragments arranged by size: 620 301 155 64 9 BfrBI ATG'CAT Cuts at: 0 549 1149 Size: 549 600 BglII A'GATC_T Cuts at: 0 1116 1149 Size: 1116 33 BlpI GC'TnA_GC Cuts at: 0 68 1149 Size: 68 1081 Bme1580I G_kGCm'C Cuts at: 0 13 617 887 1147 1149 Size: 13 604 270 260 2 Fragments arranged by size: 604 270 260 13 2 BmrI ACTGGGnnnn_n' Cuts at: 0 453 1051 1149 Size: 453 598 98 Fragments arranged by size: 598 453 98 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 322 450 454 1149 Size: 322 128 4 695 Fragments arranged by size: 695 322 128 4 BsaI GGTCTCn'nnnn_ Cuts at: 0 247 1149 Size: 247 902 BsaHI Gr'CG_yC Cuts at: 0 717 1149 Size: 717 432 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 820 850 1149 Size: 820 30 299 Fragments arranged by size: 820 299 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 618 841 1149 Size: 618 223 308 Fragments arranged by size: 618 308 223 BseYI C'CCAG_C Cuts at: 0 6 389 749 1149 Size: 6 383 360 400 Fragments arranged by size: 400 383 360 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 142 1149 Size: 142 1007 BsiHKAI G_wGCw'C Cuts at: 0 388 416 474 1028 1149 Size: 388 28 58 554 121 Fragments arranged by size: 554 388 121 58 28 BsmI GAATG_Cn' Cuts at: 0 330 1149 Size: 330 819 Bsp1286I G_dGCh'C Cuts at: 0 13 74 388 416 474 617 887 1028 Size: 13 61 314 28 58 143 270 141 Cuts at: 1028 1147 1149 Size: 119 2 Fragments arranged by size: 314 270 143 141 119 61 58 28 13 2 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 850 1029 1149 Size: 850 179 120 Fragments arranged by size: 850 179 120 BssSI C'ACGA_G Cuts at: 0 1027 1149 Size: 1027 122 BstYI r'GATC_y Cuts at: 0 115 359 1116 1149 Size: 115 244 757 33 Fragments arranged by size: 757 244 115 33 Bsu36I CC'TnA_GG Cuts at: 0 286 601 1149 Size: 286 315 548 Fragments arranged by size: 548 315 286 BtgI C'CryG_G Cuts at: 0 620 1149 Size: 620 529 BtsI GCAGTG_nn' Cuts at: 0 760 1149 Size: 760 389 DraIII CAC_nnn'GTG Cuts at: 0 882 1149 Size: 882 267 EaeI y'GGCC_r Cuts at: 0 482 803 1149 Size: 482 321 346 Fragments arranged by size: 482 346 321 EarI CTCTTCn'nnn_ Cuts at: 0 421 1149 Size: 421 728 EcoICRI GAG'CTC Cuts at: 0 386 414 472 1026 1149 Size: 386 28 58 554 123 Fragments arranged by size: 554 386 123 58 28 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 322 450 454 892 1149 Size: 322 128 4 438 257 Fragments arranged by size: 438 322 257 128 4 EcoO109I rG'GnC_Cy Cuts at: 0 282 1041 1149 Size: 282 759 108 Fragments arranged by size: 759 282 108 EcoRV GAT'ATC Cuts at: 0 580 1149 Size: 580 569 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 285 317 522 554 702 715 734 747 Size: 285 32 205 32 148 13 19 13 Cuts at: 747 1101 1108 1133 1140 1149 Size: 354 7 25 7 9 Fragments arranged by size: 354 285 205 148 32 32 25 19 13 13 9 7 7 MscI TGG'CCA Cuts at: 0 484 805 1149 Size: 484 321 344 Fragments arranged by size: 484 344 321 MslI CAynn'nnrTG Cuts at: 0 152 664 1079 1149 Size: 152 512 415 70 Fragments arranged by size: 512 415 152 70 MspA1I CmG'CkG Cuts at: 0 860 1009 1149 Size: 860 149 140 Fragments arranged by size: 860 149 140 NcoI C'CATG_G Cuts at: 0 620 1149 Size: 620 529 NsiI A_TGCA'T Cuts at: 0 551 1149 Size: 551 598 NspI r_CATG'y Cuts at: 0 553 674 1149 Size: 553 121 475 Fragments arranged by size: 553 475 121 PasI CC'CwG_GG Cuts at: 0 204 1149 Size: 204 945 PflMI CCAn_nnn'nTGG Cuts at: 0 432 1149 Size: 432 717 PfoI T'CCnGG_A Cuts at: 0 54 338 1043 1149 Size: 54 284 705 106 Fragments arranged by size: 705 284 106 54 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 820 852 1149 Size: 820 32 297 Fragments arranged by size: 820 297 32 PpuMI rG'GwC_Cy Cuts at: 0 282 1041 1149 Size: 282 759 108 Fragments arranged by size: 759 282 108 PsiI TTA'TAA Cuts at: 0 89 1149 Size: 89 1060 PspOMI G'GGCC_C Cuts at: 0 9 1149 Size: 9 1140 PstI C_TGCA'G Cuts at: 0 566 1014 1040 1149 Size: 566 448 26 109 Fragments arranged by size: 566 448 109 26 PvuII CAG'CTG Cuts at: 0 1009 1149 Size: 1009 140 SacI G_AGCT'C Cuts at: 0 388 416 474 1028 1149 Size: 388 28 58 554 121 Fragments arranged by size: 554 388 121 58 28 SapI GCTCTTCn'nnn_ Cuts at: 0 421 1149 Size: 421 728 SbfI CC_TGCA'GG Cuts at: 0 566 1040 1149 Size: 566 474 109 Fragments arranged by size: 566 474 109 SfcI C'TryA_G Cuts at: 0 562 1010 1036 1149 Size: 562 448 26 113 Fragments arranged by size: 562 448 113 26 SphI G_CATG'C Cuts at: 0 553 1149 Size: 553 596 StuI AGG'CCT Cuts at: 0 646 1149 Size: 646 503 StyI C'CwwG_G Cuts at: 0 479 620 819 1149 Size: 479 141 199 330 Fragments arranged by size: 479 330 199 141 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 763 789 1070 1149 Size: 763 26 281 79 Fragments arranged by size: 763 281 79 26 Enzymes that do cut: AarI AcuI AloI AlwNI ApaI ApoI BanI BanII BbsI BfrBI BglII BlpI Bme1580I BmrI BpmI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BssSI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV Hin4I MscI MslI MspA1I NcoI NsiI NspI PasI PflMI PfoI PpiI PpuMI PsiI PspOMI PstI PvuII SacI SapI SbfI SfcI SphI StuI StyI TaqII Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BglI BmgBI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EciI EcoNI EcoRI FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PciI PmeI PmlI PshAI PspXI PsrI PvuI RsrII SacII SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI