################################## # NO CUT ENZYMES ( 155 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT14I FbaI FseI FspI HaeII HgaI Hin1I HindIII KasI KpnI KspI MaeII MamI MfeI MflI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccII 349 CGCG CG^CG AcsI 566 AAATTC [GA]^AATT[CT] AlwNI 167 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 566 AAATTC [GA]^AATT[CT] AspHI 453 GAGCTC G[AT]GC[AT]^C BalI 620 TGGCCA TGG^CCA BanI 160 GGTGCC G^G[CT][GA]CC BanII 453 GAGCTC G[GA]GC[CT]^C BbvCI 199 GCTGAGG CCTCAGC(-5/-2) BcnI 24 CCGGG CC^[GC]GG BmyI 453 GAGCTC G[AGT]GC[ACT]^C BpmI 442 CTGGAG CTGGAG(16/14) BsaJI 62 CCGTGG C^C[ACGT][ACGT]GG BsiHKAI 453 GAGCTC G[AT]GC[AT]^C BsmFI 7 GGGAC GGGAC(10/14) BsmI 415 GCATTC GAATGC(1/-1) Bsp1286I 453 GAGCTC G[AGT]GC[ACT]^C BsrBI 614 CCGCTC CCGCTC(-3/-3) BstBI 129 TTCGAA TT^CGAA BstEII 27 GGTCACC G^GT[ACGT]ACC BstNI 366 CCTGG CC^[AT]GG BstPI 27 GGTCACC G^GT[ACGT]ACC BstUI 349 CGCG CG^CG BstXI 263 CCAAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 350 GCGC GCG^C DsaI 62 CCGTGG C^C[GA][CT]GG EaeI 620 TGGCCA [CT]^GGCC[GA] EcoO65I 27 GGTCACC G^GT[ACGT]ACC EcoRII 366 CCTGG ^CC[AT]GG EcoT22I 413 ATGCAT ATGCA^T FauI 20 CCCGC CCCGC(4/6) HhaI 350 GCGC GCG^C HinP1I 350 GCGC G^CGC HincII 303 GTTAAC GT[CT]^[GA]AC HindII 303 GTTAAC GT[CT]^[GA]AC HpaI 303 GTTAAC GTT^AAC MluNI 620 TGGCCA TGG^CCA MscI 620 TGGCCA TGG^CCA MseI 304 TTAA T^TAA MvaI 366 CCTGG CC^[AT]GG MvnI 349 CGCG CG^CG NciI 24 CCGGG CC^[GC]GG NsiI 413 ATGCAT ATGCA^T NspI 662 GCATGC [GA]CATG^[CT] NspV 129 TTCGAA TT^CGAA PstI 556 CTGCAG CTGCA^G SacI 453 GAGCTC GAGCT^C SfuI 129 TTCGAA TT^CGAA SmlI 667 CTTGAG C^T[CT][GA]AG SphI 662 GCATGC GCATG^C Tru9I 304 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AvaII 8 GGACC G^G[AT]CC 273 GGACC BfaI 371 CTAG C^TAG 437 CTAG BsmAI 135 GTCTC GTCTC(1/5) 534 GTCTC BssKI 24 CCGGG ^CC[ACGT]GG 366 CCTGG Cfr13I 8 GGACC G^G[ACGT]CC 273 GGACC HaeIII 561 GGCC GG^CC 621 GGCC HapII 24 CCGG C^CGG 78 CCGG HpaII 24 CCGG C^CGG 78 CCGG MaeI 371 CTAG C^TAG 437 CTAG MslI 57 CATCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 60 CACCGTGGTG MspI 24 CCGG C^CGG 78 CCGG MwoI 154 GCTTATGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 399 GCTAAAGAAGC NlaIII 649 CATG CATG^ 663 CATG NlaIV 7 GGGACC GG[ACGT]^[ACGT]CC 160 GGTGCC PleI 470 GAGTC GAGTC(4/5) 532 GAGTC PvuII 376 CAGCTG CAG^CTG 430 CAGCTG Sau96I 8 GGACC G^G[ACGT]CC 273 GGACC ScrFI 24 CCGGG CC^[ACGT]GG 366 CCTGG SfaNI 412 GATGC GCATC(5/9) 74 GCATC SfcI 465 CTACAG C^T[GA][CT]AG 556 CTGCAG TfiI 112 GAATC G^A[AT]TC 232 GAATC Tsp45I 28 GTCAC ^GT[GC]AC 472 GTCAC Tsp509I 182 AATT ^AATT 567 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AciI AluI BbvI BseRI BsiYI BslI BsrI BstF5I Cac8I DdeI DpnII EarI Fnu4HI FokI HinfI HphI ItaI Ksp632I MaeIII MboI MboII MnlI MspA1I NdeII Sau3AI TaqI TseI TspRI TthHB8I