################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstXI BstZ17I CelII CfoI ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MroI MslI MunI NarI NciI NdeI NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccII 257 CGCG CG^CG ApaI 389 GGGCCC GGGCC^C AspHI 441 GAGCAC G[AT]GC[AT]^C AvaII 273 GGACC G^G[AT]CC BalI 218 TGGCCA TGG^CCA BanII 389 GGGCCC G[GA]GC[CT]^C BbvCI 639 GCTGAGG CCTCAGC(-5/-2) BcgI 37 CGAAGCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 235 CTAG C^TAG BpmI 424 CTGGAG CTGGAG(16/14) BsiHKAI 441 GAGCAC G[AT]GC[AT]^C BsmFI 248 GTCCC GGGAC(10/14) BspMI 171 GCAGGT ACCTGC(4/8) BsrFI 614 GCCGGC [GA]^CCGG[CT] BstBI 59 TTCGAA TT^CGAA BstEII 474 GGTGACC G^GT[ACGT]ACC BstPI 474 GGTGACC G^GT[ACGT]ACC BstUI 257 CGCG CG^CG Cfr10I 614 GCCGGC [GA]^CCGG[CT] DraII 272 AGGACCC [GA]G^G[ACGT]CC[CT] DsaI 479 CCATGG C^C[GA][CT]GG EaeI 218 TGGCCA [CT]^GGCC[GA] EarI 282 GAAGAG CTCTTC(1/4) EcoO109I 272 AGGACCC [GA]G^G[ACGT]CC[CT] EcoO65I 474 GGTGACC G^GT[ACGT]ACC EcoT14I 479 CCATGG C^C[AT][AT]GG HapII 615 CCGG C^CGG HgaI 246 GCGTC GACGC(5/10) HpaII 615 CCGG C^CGG Ksp632I 282 GAAGAG CTCTTC(1/4) MaeI 235 CTAG C^TAG MluNI 218 TGGCCA TGG^CCA MscI 218 TGGCCA TGG^CCA MseI 416 TTAA T^TAA MspI 615 CCGG C^CGG MvnI 257 CGCG CG^CG NaeI 614 GCCGGC GCC^GGC NcoI 479 CCATGG C^CATGG NgoMIV 614 GCCGGC G^CCGGC NspV 59 TTCGAA TT^CGAA PflMI 210 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 272 AGGACCC [GA]G^G[AT]CC[CT] PstI 337 CTGCAG CTGCA^G PvuII 379 CAGCTG CAG^CTG SfuI 59 TTCGAA TT^CGAA StuI 590 AGGCCT AGG^CCT StyI 479 CCATGG C^C[AT][AT]GG Tru9I 416 TTAA T^TAA Tsp509I 63 AATT ^AATT Van91I 210 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 234 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwI 432 GATCC GGATC(4/5) 534 GGATC AocI 268 CCTCAGG CC^T[ACGT]AGG 502 CCTCAGG BseRI 238 GAGGAG GAGGAG(10/8) 370 GAGGAG BsiYI 44 CCTGCTCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 210 CCAGTCACTGG BslI 44 CCTGCTCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 210 CCAGTCACTGG BsmBI 94 GAGACG CGTCTC(1/5) 467 GAGACG BsrI 216 ACTGG ACTGG(1/-1) 210 CCAGT BstYI 431 AGATCC [GA]^GATC[CT] 534 GGATCT Bsu36I 268 CCTCAGG CC^T[ACGT]AGG 502 CCTCAGG Eco81I 268 CCTCAGG CC^T[ACGT]AGG 502 CCTCAGG FauI 7 GCGGG CCCGC(4/6) 448 GCGGG MaeIII 213 GTCAC ^GT[ACGT]AC 475 GTGAC MboII 186 GAAGA GAAGA(8/7) 282 GAAGA MflI 431 AGATCC [GA]^GATC[CT] 534 GGATCT MspA1I 379 CAGCTG C[AC]G^C[GT]G 446 CAGCGG NlaIII 50 CATG CATG^ 480 CATG SfcI 337 CTGCAG C^T[GA][CT]AG 436 CTACAG TaqI 60 TCGA T^CGA 86 TCGA Tsp45I 213 GTCAC ^GT[GC]AC 475 GTGAC TthHB8I 60 TCGA T^CGA 86 TCGA XhoII 431 AGATCC [GA]^GATC[CT] 534 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI AluI AlwNI BanI BbvI BmyI BsaJI BsmAI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MboI MnlI MvaI MwoI NdeII NlaIV Sau3AI Sau96I ScrFI SfaNI TfiI TseI TspRI