(Circular) (Six-base) MAPSORT of: pFN21AE6634.seq Check: 6725 from: 1 to: 5353 pFN21AE6634 5353 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2167 2167 Size: 5353 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3163 278 Size: 53 83 186 936 1627 2468 Fragments arranged by size: 2468 1627 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2696 1937 Size: 759 4594 Acc65I G'GTAC_C Cuts at: 2674 2674 Size: 5353 AclI AA'CG_TT Cuts at: 1563 3480 3853 1563 Size: 1917 373 3063 Fragments arranged by size: 3063 1917 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2210 2381 3416 4800 5068 1632 Size: 578 171 1035 1384 268 1917 Fragments arranged by size: 1917 1384 1035 578 268 171 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5156 AflIII A'CryG_T Cuts at: 1203 4252 5229 1203 Size: 3049 977 1327 Fragments arranged by size: 3049 1327 977 AgeI A'CCGG_T Cuts at: 4184 4184 Size: 5353 AhdI GACnn_n'nnGTC Cuts at: 4082 4082 Size: 5353 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5353 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5353 AlwNI CAG_nnn'CTG Cuts at: 2161 2221 2341 2350 3096 4668 2161 Size: 60 120 9 746 1572 2846 Fragments arranged by size: 2846 1572 746 120 60 9 ApaI G_GGCC'C Cuts at: 2398 2398 Size: 5353 ApaLI G'TGCA_C Cuts at: 3410 4566 3410 Size: 1156 4197 ApoI r'AATT_y Cuts at: 1493 2662 2888 3040 5149 1493 Size: 1169 226 152 2109 1697 Fragments arranged by size: 2109 1697 1169 226 152 AseI AT'TA_AT Cuts at: 160 1032 3907 160 Size: 872 2875 1606 Fragments arranged by size: 2875 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5353 AvaI C'yCGr_G Cuts at: 1870 1943 2678 1870 Size: 73 735 4545 Fragments arranged by size: 4545 735 73 BamHI G'GATC_C Cuts at: 2683 3050 2683 Size: 367 4986 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2104 2179 2503 2674 4129 Size: 325 217 627 317 75 324 171 1455 Cuts at: 4129 618 Size: 1842 Fragments arranged by size: 1842 1455 627 325 324 317 217 171 75 BanII G_rGCy'C Cuts at: 1391 2398 2672 1391 Size: 1007 274 4072 Fragments arranged by size: 4072 1007 274 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4382 BbvCI CC'TCA_GC Cuts at: 2645 2645 Size: 5353 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2031 2065 3544 3578 1903 Size: 34 50 34 10 34 1479 34 3678 Fragments arranged by size: 3678 1479 50 34 34 34 34 10 BciVI GTATCCnnnnn_n' Cuts at: 3245 4455 3245 Size: 1210 4143 BclI T'GATC_A Cuts at: 1548 1548 Size: 5353 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3964 136 Size: 107 122 71 631 605 337 1955 1525 Fragments arranged by size: 1955 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5348 5348 Size: 5353 BlpI GC'TnA_GC Cuts at: 2770 2770 Size: 5353 Bme1580I G_kGCm'C Cuts at: 1790 2109 2398 3414 4570 1790 Size: 319 289 1016 1156 2573 Fragments arranged by size: 2573 1156 1016 319 289 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5353 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3084 4042 442 Size: 416 53 83 399 24 1667 958 1753 Fragments arranged by size: 1753 1667 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5353 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2450 4013 1239 Size: 1211 1563 2579 Fragments arranged by size: 2579 1563 1211 Bpu10I CC'TnA_GC Cuts at: 2645 2645 Size: 5353 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3478 4343 4641 3478 Size: 865 298 4190 Fragments arranged by size: 4190 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4016 882 Size: 617 2517 2219 Fragments arranged by size: 2517 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3144 493 Size: 711 1940 2702 Fragments arranged by size: 2702 1940 711 BsaBI GATnn'nnATC Cuts at: 5347 5347 Size: 5353 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3160 3542 4177 275 Size: 53 83 186 936 1627 382 635 1451 Fragments arranged by size: 1627 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3786 4184 4458 4605 5016 3786 Size: 398 274 147 411 4123 Fragments arranged by size: 4123 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2463 2493 1178 Size: 30 165 30 1060 30 4038 Fragments arranged by size: 4038 1060 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2258 2390 1186 Size: 1072 132 4149 Fragments arranged by size: 4149 1072 132 BseYI C'CCAG_C Cuts at: 4556 5052 4556 Size: 496 4857 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4780 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2803 3564 4592 1540 Size: 275 186 802 761 1028 2301 Fragments arranged by size: 2301 1028 802 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2450 3414 3499 4570 2450 Size: 964 85 1071 3233 Fragments arranged by size: 3233 1071 964 85 BsmI GAATG_Cn' Cuts at: 2864 2957 2864 Size: 93 5260 BsmBI CGTCTCn'nnnn_ Cuts at: 2093 2466 2093 Size: 373 4980 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2109 2398 2450 2672 3414 3499 4570 Size: 399 319 289 52 222 742 85 1071 Cuts at: 4570 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 319 289 222 85 52 BspEI T'CCGG_A Cuts at: 5016 5016 Size: 5353 BspHI T'CATG_A Cuts at: 3242 4972 3242 Size: 1730 3623 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2167 2708 844 Size: 1323 541 3489 Fragments arranged by size: 3489 1323 541 BsrBI CCG'CTC Cuts at: 1394 1823 3240 1394 Size: 429 1417 3507 Fragments arranged by size: 3507 1417 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3848 4022 65 Size: 1183 2600 174 1396 Fragments arranged by size: 2600 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2619 3997 4184 1700 Size: 110 809 1378 187 2869 Fragments arranged by size: 2869 1378 809 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5353 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5353 BssSI C'ACGA_G Cuts at: 3413 4425 3413 Size: 1012 4341 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5353 BstBI TT'CG_AA Cuts at: 2065 4157 2065 Size: 2092 3261 BstEII G'GTnAC_C Cuts at: 1184 2479 1184 Size: 1295 4058 BstYI r'GATC_y Cuts at: 1972 2436 2539 2683 3050 3435 3452 4904 4990 Size: 464 103 144 367 385 17 1452 86 Cuts at: 4990 5002 5348 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 464 385 367 346 144 103 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2274 2508 2274 Size: 234 5119 BtgI C'CryG_G Cuts at: 513 1065 2484 513 Size: 552 1419 3382 Fragments arranged by size: 3382 1419 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2305 487 Size: 683 56 1079 3535 Fragments arranged by size: 3535 1079 683 56 BtsI GCAGTG_nn' Cuts at: 2869 3663 3683 2869 Size: 794 20 4539 Fragments arranged by size: 4539 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5353 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5318 DraI TTT'AAA Cuts at: 2657 2657 Size: 5353 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4360 809 Size: 507 3044 1802 Fragments arranged by size: 3044 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2223 2800 3689 Size: 54 1425 50 275 18 393 577 889 Cuts at: 3689 5318 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 577 393 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2800 1537 Size: 275 988 4090 Fragments arranged by size: 4090 988 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2282 3283 1353 Size: 929 1001 3423 Fragments arranged by size: 3423 1001 929 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3940 4314 4460 387 Size: 1064 138 2351 374 146 1280 Fragments arranged by size: 2351 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2210 2381 2450 3416 4013 4800 5068 Size: 393 578 171 69 966 597 787 268 Cuts at: 5068 1239 Size: 1524 Fragments arranged by size: 1524 966 787 597 578 393 268 171 69 EcoO109I rG'GnC_Cy Cuts at: 2278 2278 Size: 5353 EcoRI G'AATT_C Cuts at: 2662 3040 2662 Size: 378 4975 FspI TGC'GCA Cuts at: 3859 3859 Size: 5353 HaeII r_GCGC'y Cuts at: 1678 4500 1678 Size: 2822 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3994 4026 4068 4100 1373 Size: 32 135 32 2422 32 42 32 2626 Fragments arranged by size: 2626 2422 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2697 2943 669 Size: 988 281 759 246 3079 Fragments arranged by size: 3079 988 759 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5353 HpaI GTT'AAC Cuts at: 2943 2943 Size: 5353 KpnI G_GTAC'C Cuts at: 2678 2678 Size: 5353 MfeI C'AATT_G Cuts at: 2952 2952 Size: 5353 MluI A'CGCG_T Cuts at: 5229 5229 Size: 5353 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4467 4651 1305 Size: 3162 184 2007 Fragments arranged by size: 3162 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2225 10 Size: 54 1425 343 393 3138 Fragments arranged by size: 3138 1425 393 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3311 3670 3829 518 Size: 648 2145 359 159 2042 Fragments arranged by size: 2145 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2386 2453 2769 3446 4594 4839 1606 Size: 780 67 316 677 1148 245 2120 Fragments arranged by size: 2120 1148 780 677 316 245 67 NaeI GCC'GGC Cuts at: 2621 2621 Size: 5353 NcoI C'CATG_G Cuts at: 513 1065 2484 513 Size: 552 1419 3382 Fragments arranged by size: 3382 1419 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5353 NgoMIV G'CCGG_C Cuts at: 2619 2619 Size: 5353 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5353 NotI GC'GGCC_GC Cuts at: 2800 2800 Size: 5353 NspI r_CATG'y Cuts at: 1132 1229 2711 4256 1132 Size: 97 1482 1545 2229 Fragments arranged by size: 2229 1545 1482 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5353 PciI A'CATG_T Cuts at: 4252 4252 Size: 5353 PflMI CCAn_nnn'nTGG Cuts at: 1108 2221 1108 Size: 1113 4240 PmeI GTTT'AAAC Cuts at: 2657 2657 Size: 5353 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3422 3454 4935 4967 3422 Size: 32 1481 32 3808 Fragments arranged by size: 3808 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2278 2278 Size: 5353 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5353 PsiI TTA'TAA Cuts at: 2923 2923 Size: 5353 PspOMI G'GGCC_C Cuts at: 2394 2394 Size: 5353 PstI C_TGCA'G Cuts at: 830 2346 2705 830 Size: 1516 359 3478 Fragments arranged by size: 3478 1516 359 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5353 PvuII CAG'CTG Cuts at: 2386 2386 Size: 5353 SalI G'TCGA_C Cuts at: 1936 2695 1936 Size: 759 4594 SbfI CC_TGCA'GG Cuts at: 2705 2705 Size: 5353 ScaI AGT'ACT Cuts at: 3601 5313 3601 Size: 1712 3641 SfcI C'TryA_G Cuts at: 826 1045 2342 2441 2701 3836 4517 4708 826 Size: 219 1297 99 260 1135 681 191 1471 Fragments arranged by size: 1471 1297 1135 681 260 219 191 99 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5353 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5353 SmaI CCC'GGG Cuts at: 1872 2680 1872 Size: 808 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3457 4358 4620 820 Size: 197 926 1514 901 262 1553 Fragments arranged by size: 1553 1514 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3144 493 Size: 2651 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5353 SphI G_CATG'C Cuts at: 1132 2711 1132 Size: 1579 3774 SspI AAT'ATT Cuts at: 5 52 3277 5 Size: 47 3225 2081 Fragments arranged by size: 3225 2081 47 StuI AGG'CCT Cuts at: 1517 2597 1517 Size: 1080 4273 StyI C'CwwG_G Cuts at: 513 1065 2484 2792 513 Size: 552 1419 308 3074 Fragments arranged by size: 3074 1419 552 308 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2650 3380 3397 3550 3735 4180 325 Size: 1255 1070 730 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1070 730 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3599 5311 96 Size: 274 80 33 51 1443 1622 1712 138 Fragments arranged by size: 1712 1622 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2463 2495 5313 5345 2463 Size: 32 2818 32 2471 Fragments arranged by size: 2818 2471 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5353 XbaI T'CTAG_A Cuts at: 2239 2689 2239 Size: 450 4903 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5353 XmaI C'CCGG_G Cuts at: 1870 2678 1870 Size: 808 4545 XmnI GAAnn'nnTTC Cuts at: 3482 3482 Size: 5353 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3161 276 Size: 53 83 186 936 1627 2468 Fragments arranged by size: 2468 1627 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI