(Circular) (Six-base) MAPSORT of: pFN21AE9669.seq Check: 7247 from: 1 to: 13696 pFN21AE9669 13696 bp With 182 enzymes: SgfI * October 14, 2011 09:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5515 5515 Size: 13696 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5572 9602 11506 278 Size: 53 83 186 936 4036 4030 1904 2468 Fragments arranged by size: 4036 4030 2468 1904 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2379 11039 1937 Size: 442 8660 4594 Fragments arranged by size: 8660 4594 442 Acc65I G'GTAC_C Cuts at: 2458 5994 11017 2458 Size: 3536 5023 5137 Fragments arranged by size: 5137 5023 3536 AclI AA'CG_TT Cuts at: 1563 11823 12196 1563 Size: 10260 373 3063 Fragments arranged by size: 10260 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4236 4305 5354 5422 5460 5808 5930 6774 Size: 2604 69 1049 68 38 348 122 844 Cuts at: 6774 7217 7362 7614 8032 8871 9809 10262 10769 Size: 443 145 252 418 839 938 453 507 Cuts at: 10769 11759 13143 13411 1632 Size: 990 1384 268 1917 Fragments arranged by size: 2604 1917 1384 1049 990 938 844 839 507 453 443 418 348 268 252 145 122 69 68 38 AfeI AGC'GCT Cuts at: 4277 4277 Size: 13696 AflII C'TTAA_G Cuts at: 820 1017 8237 820 Size: 197 7220 6279 Fragments arranged by size: 7220 6279 197 AflIII A'CryG_T Cuts at: 1203 7736 10428 12595 13572 1203 Size: 6533 2692 2167 977 1327 Fragments arranged by size: 6533 2692 2167 1327 977 AgeI A'CCGG_T Cuts at: 12527 12527 Size: 13696 AhdI GACnn_n'nnGTC Cuts at: 5274 7737 8686 8752 12425 5274 Size: 2463 949 66 3673 6545 Fragments arranged by size: 6545 3673 2463 949 66 AleI CACnn'nnGTG Cuts at: 1166 3111 9062 1166 Size: 1945 5951 5800 Fragments arranged by size: 5951 5800 1945 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5897 8198 1184 Size: 4713 2301 6682 Fragments arranged by size: 6682 4713 2301 AlwNI CAG_nnn'CTG Cuts at: 2699 4334 5346 7067 8684 8786 9428 10592 11439 Size: 1635 1012 1721 1617 102 642 1164 847 Cuts at: 11439 13011 2699 Size: 1572 3384 Fragments arranged by size: 3384 1721 1635 1617 1572 1164 1012 847 642 102 ApaI G_GGCC'C Cuts at: 2444 2535 3359 2444 Size: 91 824 12781 Fragments arranged by size: 12781 824 91 ApaLI G'TGCA_C Cuts at: 6414 11753 12909 6414 Size: 5339 1156 7201 Fragments arranged by size: 7201 5339 1156 ApoI r'AATT_y Cuts at: 1493 2995 4174 4478 4557 5105 5498 5677 5796 Size: 1502 1179 304 79 548 393 179 119 Cuts at: 5796 6256 6446 6589 7544 7613 7621 8803 9373 Size: 460 190 143 955 69 8 1182 570 Cuts at: 9373 10037 11005 11231 11383 13492 1493 Size: 664 968 226 152 2109 1697 Fragments arranged by size: 2109 1697 1502 1182 1179 968 955 664 570 548 460 393 304 226 190 179 152 143 119 79 69 8 AseI AT'TA_AT Cuts at: 160 1032 12250 160 Size: 872 11218 1606 Fragments arranged by size: 11218 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 13696 AvaI C'yCGr_G Cuts at: 1870 1943 3565 7032 7836 8853 10702 11021 1870 Size: 73 1622 3467 804 1017 1849 319 4545 Fragments arranged by size: 4545 3467 1849 1622 1017 804 319 73 BamHI G'GATC_C Cuts at: 3832 7320 9708 11026 11393 3832 Size: 3488 2388 1318 367 6135 Fragments arranged by size: 6135 3488 2388 1318 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2341 2458 2740 3326 3403 Size: 325 217 627 554 117 282 586 77 Cuts at: 3403 5994 9012 9943 9991 11017 12472 618 Size: 2591 3018 931 48 1026 1455 1842 Fragments arranged by size: 3018 2591 1842 1455 1026 931 627 586 554 325 282 217 117 77 48 BanII G_rGCy'C Cuts at: 1391 2444 2479 2535 3359 3687 3999 7855 9102 Size: 1053 35 56 824 328 312 3856 1247 Cuts at: 9102 10802 11015 1391 Size: 1700 213 4072 Fragments arranged by size: 4072 3856 1700 1247 1053 824 328 312 213 56 35 BbeI G_GCGC'C Cuts at: 9016 9995 9016 Size: 979 12717 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3576 4293 4752 5979 7569 7817 8757 Size: 971 1677 717 459 1227 1590 248 940 Cuts at: 8757 10521 10563 10926 928 Size: 1764 42 363 3698 Fragments arranged by size: 3698 1764 1677 1590 1227 971 940 717 459 363 248 42 BbvCI CC'TCA_GC Cuts at: 2553 3303 9621 10830 2553 Size: 750 6318 1209 5419 Fragments arranged by size: 6318 5419 1209 750 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2918 2952 3359 3393 9227 9261 9453 Size: 34 981 34 407 34 5834 34 192 Cuts at: 9453 9487 11887 11921 1903 Size: 34 2400 34 3678 Fragments arranged by size: 5834 3678 2400 981 407 192 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3255 7948 11588 12798 3255 Size: 4693 3640 1210 4153 Fragments arranged by size: 4693 4153 3640 1210 BclI T'GATC_A Cuts at: 1548 2212 6466 7005 1548 Size: 664 4254 539 8239 Fragments arranged by size: 8239 4254 664 539 BfrBI ATG'CAT Cuts at: 2245 3148 2245 Size: 903 12793 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2658 10323 12307 Size: 107 122 71 631 605 986 7665 1984 Cuts at: 12307 136 Size: 1525 Fragments arranged by size: 7665 1984 1525 986 631 605 122 107 71 BglII A'GATC_T Cuts at: 4537 7281 13691 4537 Size: 2744 6410 4542 Fragments arranged by size: 6410 4542 2744 BlpI GC'TnA_GC Cuts at: 3738 8895 9096 11113 3738 Size: 5157 201 2017 6321 Fragments arranged by size: 6321 5157 2017 201 Bme1580I G_kGCm'C Cuts at: 1790 2346 2444 2535 2721 3359 3426 3619 6418 Size: 556 98 91 186 638 67 193 2799 Cuts at: 6418 11757 12913 1790 Size: 5339 1156 2573 Fragments arranged by size: 5339 2799 2573 1156 638 556 193 186 98 91 67 BmgBI CAC'GTC Cuts at: 1318 7739 10218 1318 Size: 6421 2479 4796 Fragments arranged by size: 6421 4796 2479 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3105 3429 3831 Size: 416 53 83 399 24 1688 324 402 Cuts at: 3831 4360 4397 5104 6308 8694 10128 10751 11427 Size: 529 37 707 1204 2386 1434 623 676 Cuts at: 11427 12385 442 Size: 958 1753 Fragments arranged by size: 2386 1753 1688 1434 1204 958 707 676 623 529 416 402 399 324 83 53 37 24 BmtI G_CTAG'C Cuts at: 1056 7523 10670 1056 Size: 6467 3147 4082 Fragments arranged by size: 6467 4082 3147 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6050 8173 8656 10966 6050 Size: 2123 483 2310 8780 Fragments arranged by size: 8780 2310 2123 483 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4313 4476 5399 5820 5834 6170 8451 8759 Size: 3074 163 923 421 14 336 2281 308 Cuts at: 8759 10035 10271 10280 10289 10317 10719 10786 12356 Size: 1276 236 9 9 28 402 67 1570 Cuts at: 12356 1239 Size: 2579 Fragments arranged by size: 3074 2579 2281 1570 1276 923 421 402 336 308 236 163 67 28 14 9 9 Bpu10I CC'TnA_GC Cuts at: 2553 3303 5280 6192 6534 9621 10830 2553 Size: 750 1977 912 342 3087 1209 5419 Fragments arranged by size: 5419 3087 1977 1209 912 750 342 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5351 5690 7285 9331 11821 12686 12984 5351 Size: 339 1595 2046 2490 865 298 6063 Fragments arranged by size: 6063 2490 2046 1595 865 339 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3456 7260 9307 9647 10850 12359 882 Size: 617 1957 3804 2047 340 1203 1509 2219 Fragments arranged by size: 3804 2219 2047 1957 1509 1203 617 340 BsaAI yAC'GTr Cuts at: 493 1204 2588 2716 4679 4755 11487 493 Size: 711 1384 128 1963 76 6732 2702 Fragments arranged by size: 6732 2702 1963 1384 711 128 76 BsaBI GATnn'nnATC Cuts at: 3064 6465 13690 3064 Size: 3401 7225 3070 Fragments arranged by size: 7225 3401 3070 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5569 6781 9013 9599 Size: 53 83 186 936 4036 1212 2232 586 Cuts at: 9599 9992 11503 11885 12520 275 Size: 393 1511 382 635 1451 Fragments arranged by size: 4036 2232 1511 1451 1212 936 635 586 393 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4659 7052 10510 12129 12527 12801 12948 13359 4659 Size: 2393 3458 1619 398 274 147 411 4996 Fragments arranged by size: 4996 3458 2393 1619 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2414 2444 2793 2823 3217 Size: 30 165 30 1011 30 349 30 394 Cuts at: 3217 3247 3532 3562 4449 4479 4689 4719 6044 Size: 30 285 30 887 30 210 30 1325 Cuts at: 6044 6074 6805 6835 8108 8138 9136 9166 1178 Size: 30 731 30 1273 30 998 30 5708 Fragments arranged by size: 5708 1325 1273 1011 998 887 731 394 349 285 210 165 30 30 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3219 3222 4197 4200 4328 4475 4808 6443 Size: 2033 3 975 3 128 147 333 1635 Cuts at: 6443 8113 8162 8454 8546 9100 9144 9220 9223 Size: 1670 49 292 92 554 44 76 3 Cuts at: 9223 9226 9229 9550 10550 10895 1186 Size: 3 3 321 1000 345 3987 Fragments arranged by size: 3987 2033 1670 1635 1000 975 554 345 333 321 292 147 128 92 76 49 44 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2721 2732 3257 4337 8220 8224 8545 8769 9279 Size: 11 525 1080 3883 4 321 224 510 Cuts at: 9279 10768 12899 13395 2721 Size: 1489 2131 496 3022 Fragments arranged by size: 3883 3022 2131 1489 1080 525 510 496 321 224 11 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2523 2783 6723 7145 9630 9779 1163 Size: 573 787 260 3940 422 2485 149 5080 Fragments arranged by size: 5080 3940 2485 787 573 422 260 149 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6019 10197 11146 11907 12935 1540 Size: 275 186 4018 4178 949 761 1028 2301 Fragments arranged by size: 4178 4018 2301 1028 949 761 275 186 BsiHKAI G_wGCw'C Cuts at: 6418 7112 7855 8837 9102 11757 11842 12913 6418 Size: 694 743 982 265 2655 85 1071 7201 Fragments arranged by size: 7201 2655 1071 982 743 694 265 85 BsmI GAATG_Cn' Cuts at: 3670 3739 8091 9844 10795 11207 11300 3670 Size: 69 4352 1753 951 412 93 6066 Fragments arranged by size: 6066 4352 1753 951 412 93 69 BsmBI CGTCTCn'nnnn_ Cuts at: 2307 4240 5560 5576 9156 2307 Size: 1933 1320 16 3580 6847 Fragments arranged by size: 6847 3580 1933 1320 16 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2346 2444 2479 2535 2721 3359 3426 Size: 399 556 98 35 56 186 638 67 Cuts at: 3426 3619 3687 3999 6418 7112 7855 8837 9102 Size: 193 68 312 2419 694 743 982 265 Cuts at: 9102 10802 11015 11757 11842 12913 1391 Size: 1700 213 742 85 1071 2174 Fragments arranged by size: 2419 2174 1700 1071 982 743 742 694 638 556 399 312 265 213 193 186 98 85 68 67 56 35 BspEI T'CCGG_A Cuts at: 13359 13359 Size: 13696 BspHI T'CATG_A Cuts at: 8905 9582 11585 13315 8905 Size: 677 2003 1730 9286 Fragments arranged by size: 9286 2003 1730 677 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3771 5515 6152 8194 8405 11051 844 Size: 2927 1744 637 2042 211 2646 3489 Fragments arranged by size: 3489 2927 2646 2042 1744 637 211 BsrBI CCG'CTC Cuts at: 1394 1823 4762 11583 1394 Size: 429 2939 6821 3507 Fragments arranged by size: 6821 3507 2939 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3213 5862 8827 12191 12365 65 Size: 1183 1965 2649 2965 3364 174 1396 Fragments arranged by size: 3364 2965 2649 1965 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2518 7117 7746 7818 12340 12527 1700 Size: 110 708 4599 629 72 4522 187 2869 Fragments arranged by size: 4599 4522 2869 708 629 187 110 72 BsrGI T'GTAC_A Cuts at: 96 6098 96 Size: 6002 7694 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 13696 BssSI C'ACGA_G Cuts at: 11756 12768 11756 Size: 1012 12684 BstAPI GCAn_nnn'nTGC Cuts at: 794 3655 4064 4325 4334 9617 10349 794 Size: 2861 409 261 9 5283 732 4141 Fragments arranged by size: 5283 4141 2861 732 409 261 9 BstBI TT'CG_AA Cuts at: 12500 12500 Size: 13696 BstEII G'GTnAC_C Cuts at: 1184 2087 9625 1184 Size: 903 7538 5255 Fragments arranged by size: 7538 5255 903 BstXI CCAn_nnnn'nTGG Cuts at: 3113 3323 5870 6594 8274 9064 10021 3113 Size: 210 2547 724 1680 790 957 6788 Fragments arranged by size: 6788 2547 1680 957 790 724 210 BstYI r'GATC_y Cuts at: 1972 2011 3832 4537 5197 6388 6471 7000 7281 Size: 39 1821 705 660 1191 83 529 281 Cuts at: 7281 7320 8193 8260 8881 9708 10006 10619 11026 Size: 39 873 67 621 827 298 613 407 Cuts at: 11026 11393 11778 11795 13247 13333 13345 13691 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1821 1452 1191 873 827 705 660 621 613 529 407 385 367 346 298 281 86 83 67 39 39 17 12 Bsu36I CC'TnA_GG Cuts at: 2337 2692 3162 8937 2337 Size: 355 470 5775 7096 Fragments arranged by size: 7096 5775 470 355 BtgI C'CryG_G Cuts at: 513 1065 2004 2347 2797 3330 5050 5297 6776 Size: 552 939 343 450 533 1720 247 1479 Cuts at: 6776 6883 7650 9105 10191 10794 513 Size: 107 767 1455 1086 603 3415 Fragments arranged by size: 3415 1720 1479 1455 1086 939 767 603 552 533 450 343 247 107 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 10380 10887 487 Size: 683 56 9154 507 3296 Fragments arranged by size: 9154 3296 683 507 56 BtsI GCAGTG_nn' Cuts at: 2362 3551 6129 6711 8835 8988 11212 12006 12026 Size: 1189 2578 582 2124 153 2224 794 20 Cuts at: 12026 2362 Size: 4032 Fragments arranged by size: 4032 2578 2224 2124 1189 794 582 153 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 13696 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5683 5718 529 Size: 35 5119 35 8507 Fragments arranged by size: 8507 5119 35 35 DraI TTT'AAA Cuts at: 5812 10846 11000 5812 Size: 5034 154 8508 Fragments arranged by size: 8508 5034 154 DraIII CAC_nnn'GTG Cuts at: 2761 5392 5929 10937 2761 Size: 2631 537 5008 5520 Fragments arranged by size: 5520 5008 2631 537 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 9891 10903 12703 809 Size: 507 8575 1012 1800 1802 Fragments arranged by size: 8575 1802 1800 1012 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2701 3496 5920 Size: 54 1425 50 275 18 871 795 2424 Cuts at: 5920 6016 7024 7143 8061 8999 9112 10194 11143 Size: 96 1008 119 918 938 113 1082 949 Cuts at: 11143 12032 13661 8 Size: 889 1629 43 Fragments arranged by size: 2424 1629 1425 1082 1008 949 938 918 889 871 795 275 119 113 96 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6016 10194 11143 1537 Size: 275 4204 4178 949 4090 Fragments arranged by size: 4204 4178 4090 949 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3743 4238 4262 5441 5514 5784 5799 6331 Size: 2390 495 24 1179 73 270 15 532 Cuts at: 6331 7903 7908 8571 8712 8721 8754 9077 9527 Size: 1572 5 663 141 9 33 323 450 Cuts at: 9527 10147 10715 10935 11626 1353 Size: 620 568 220 691 3423 Fragments arranged by size: 3423 2390 1572 1179 691 663 620 568 532 495 450 323 270 220 141 73 33 24 15 9 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6026 7289 12283 12657 12803 387 Size: 1064 138 4437 1263 4994 374 146 1280 Fragments arranged by size: 4994 4437 1280 1263 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 7853 9100 7853 Size: 1247 12449 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4236 4305 4313 4476 5354 5399 5422 Size: 393 2604 69 8 163 878 45 23 Cuts at: 5422 5460 5808 5820 5834 5930 6170 6774 7217 Size: 38 348 12 14 96 240 604 443 Cuts at: 7217 7362 7614 8032 8451 8759 8871 9809 10035 Size: 145 252 418 419 308 112 938 226 Cuts at: 10035 10262 10271 10280 10289 10317 10719 10769 10786 Size: 227 9 9 9 28 402 50 17 Cuts at: 10786 11759 12356 13143 13411 1239 Size: 973 597 787 268 1524 Fragments arranged by size: 2604 1524 973 938 878 787 604 597 443 419 418 402 393 348 308 268 252 240 227 226 163 145 112 96 69 50 45 38 28 23 17 14 12 9 9 9 8 EcoNI CCTnn'n_nnAGG Cuts at: 2666 2960 6038 6265 7169 8005 2666 Size: 294 3078 227 904 836 8357 Fragments arranged by size: 8357 3078 904 836 294 227 EcoO109I rG'GnC_Cy Cuts at: 2440 2441 2531 2532 2659 2696 3356 3912 4228 Size: 1 90 1 127 37 660 556 316 Cuts at: 4228 5223 6405 7216 7801 10838 2440 Size: 995 1182 811 585 3037 5298 Fragments arranged by size: 5298 3037 1182 995 811 660 585 556 316 127 90 37 1 1 EcoRI G'AATT_C Cuts at: 4478 4557 5105 11005 11383 4478 Size: 79 548 5900 378 6791 Fragments arranged by size: 6791 5900 548 378 79 EcoRV GAT'ATC Cuts at: 2942 2942 Size: 13696 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 8841 9106 9418 9485 8841 Size: 265 312 67 13052 Fragments arranged by size: 13052 312 265 67 FspI TGC'GCA Cuts at: 12202 12202 Size: 13696 HaeII r_GCGC'y Cuts at: 1678 4279 5305 9016 9995 12843 1678 Size: 2601 1026 3711 979 2848 2531 Fragments arranged by size: 3711 2848 2601 2531 1026 979 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2414 2446 4337 4369 4699 Size: 32 135 32 842 32 1891 32 330 Cuts at: 4699 4731 5260 5292 6044 6076 6425 6457 7026 Size: 32 529 32 752 32 349 32 569 Cuts at: 7026 7058 7723 7755 8006 8038 8108 8140 8329 Size: 32 665 32 251 32 70 32 189 Cuts at: 8329 8361 8672 8704 8738 8770 9244 9276 12337 Size: 32 311 32 34 32 474 32 3061 Cuts at: 12337 12369 12411 12443 1373 Size: 32 42 32 2626 Fragments arranged by size: 3061 2626 1891 842 752 665 569 529 474 349 330 311 251 189 135 70 42 34 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2974 3515 4147 4657 7045 11040 Size: 988 281 1036 541 632 510 2388 3995 Cuts at: 11040 11286 669 Size: 246 3079 Fragments arranged by size: 3995 3079 2388 1036 988 632 541 510 281 246 HindIII A'AGCT_T Cuts at: 748 6988 8254 9633 748 Size: 6240 1266 1379 4811 Fragments arranged by size: 6240 4811 1379 1266 HpaI GTT'AAC Cuts at: 2974 11286 2974 Size: 8312 5384 KasI G'GCGC_C Cuts at: 9012 9991 9012 Size: 979 12717 KpnI G_GTAC'C Cuts at: 2462 5998 11021 2462 Size: 3536 5023 5137 Fragments arranged by size: 5137 5023 3536 MfeI C'AATT_G Cuts at: 4948 11295 4948 Size: 6347 7349 MluI A'CGCG_T Cuts at: 13572 13572 Size: 13696 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3660 4380 4902 5144 5337 8315 8428 8736 Size: 2355 720 522 242 193 2978 113 308 Cuts at: 8736 12810 12994 1305 Size: 4074 184 2007 Fragments arranged by size: 4074 2978 2355 2007 720 522 308 242 193 184 113 MscI TGG'CCA Cuts at: 10 64 1489 1832 2703 3498 5922 7026 7145 Size: 54 1425 343 871 795 2424 1104 119 Cuts at: 7145 8063 9001 10 Size: 918 938 4705 Fragments arranged by size: 4705 2424 1425 1104 938 918 871 795 343 119 54 MslI CAynn'nnrTG Cuts at: 518 1166 3111 5489 5738 6944 7410 9062 9269 Size: 648 1945 2378 249 1206 466 1652 207 Cuts at: 9269 11654 12013 12172 518 Size: 2385 359 159 2042 Fragments arranged by size: 2385 2378 2042 1945 1652 1206 648 466 359 249 207 159 MspA1I CmG'CkG Cuts at: 1606 7019 7149 8224 8549 8601 9202 9691 10193 Size: 5413 130 1075 325 52 601 489 502 Cuts at: 10193 10202 10309 10349 10469 11112 11789 12937 13182 Size: 9 107 40 120 643 677 1148 245 Cuts at: 13182 1606 Size: 2120 Fragments arranged by size: 5413 2120 1148 1075 677 643 601 502 489 325 245 130 120 107 52 40 9 NaeI GCC'GGC Cuts at: 2520 7748 2520 Size: 5228 8468 NarI GG'CG_CC Cuts at: 9013 9992 9013 Size: 979 12717 NcoI C'CATG_G Cuts at: 513 1065 2004 2347 2797 3330 5050 5297 7650 Size: 552 939 343 450 533 1720 247 2353 Cuts at: 7650 9105 513 Size: 1455 5104 Fragments arranged by size: 5104 2353 1720 1455 939 552 533 450 343 247 NdeI CA'TA_TG Cuts at: 387 6001 387 Size: 5614 8082 NgoMIV G'CCGG_C Cuts at: 2518 7746 2518 Size: 5228 8468 NheI G'CTAG_C Cuts at: 1052 7519 10666 1052 Size: 6467 3147 4082 Fragments arranged by size: 6467 4082 3147 NotI GC'GGCC_GC Cuts at: 10194 11143 10194 Size: 949 12747 NsiI A_TGCA'T Cuts at: 2247 3150 2247 Size: 903 12793 NspI r_CATG'y Cuts at: 1132 1229 2392 2563 3370 3397 4963 7237 7519 Size: 97 1163 171 807 27 1566 2274 282 Cuts at: 7519 7664 11054 12599 1132 Size: 145 3390 1545 2229 Fragments arranged by size: 3390 2274 2229 1566 1545 1163 807 282 171 145 97 27 PasI CC'CwG_GG Cuts at: 1348 2285 2445 5203 1348 Size: 937 160 2758 9841 Fragments arranged by size: 9841 2758 937 160 PciI A'CATG_T Cuts at: 12595 12595 Size: 13696 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 13696 PfoI T'CCnGG_A Cuts at: 2014 3477 7170 9682 10333 10735 2014 Size: 1463 3693 2512 651 402 4975 Fragments arranged by size: 4975 3693 2512 1463 651 402 PmeI GTTT'AAAC Cuts at: 11000 11000 Size: 13696 PmlI CAC'GTG Cuts at: 2588 2716 4755 2588 Size: 128 2039 11529 Fragments arranged by size: 11529 2039 128 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4414 4446 10556 10588 11765 11797 13278 13310 4414 Size: 32 6110 32 1177 32 1481 32 4800 Fragments arranged by size: 6110 4800 1481 1177 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2696 3912 4228 5223 7216 10838 2696 Size: 1216 316 995 1993 3622 5554 Fragments arranged by size: 5554 3622 1993 1216 995 316 PshAI GACnn'nnGTC Cuts at: 1613 2299 1613 Size: 686 13010 PsiI TTA'TAA Cuts at: 11266 11266 Size: 13696 PspOMI G'GGCC_C Cuts at: 2440 2531 3355 2440 Size: 91 824 12781 Fragments arranged by size: 12781 824 91 PspXI vC'TCGA_Gb Cuts at: 7032 7032 Size: 13696 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3474 3506 3474 Size: 32 13664 PstI C_TGCA'G Cuts at: 830 3558 6935 7354 10903 11048 830 Size: 2728 3377 419 3549 145 3478 Fragments arranged by size: 3549 3478 3377 2728 419 145 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 13696 PvuII CAG'CTG Cuts at: 7149 8224 8549 9691 10309 10349 10469 7149 Size: 1075 325 1142 618 40 120 10376 Fragments arranged by size: 10376 1142 1075 618 325 120 40 SacI G_AGCT'C Cuts at: 7855 9102 7855 Size: 1247 12449 SacII CC_GC'GG Cuts at: 10194 10194 Size: 13696 SalI G'TCGA_C Cuts at: 1936 11038 1936 Size: 9102 4594 SanDI GG'GwC_CC Cuts at: 3912 3912 Size: 13696 SapI GCTCTTCn'nnn_ Cuts at: 5514 10935 5514 Size: 5421 8275 SbfI CC_TGCA'GG Cuts at: 11048 11048 Size: 13696 ScaI AGT'ACT Cuts at: 11944 13656 11944 Size: 1712 11984 SexAI A'CCwGG_T Cuts at: 6654 6654 Size: 13696 SfcI C'TryA_G Cuts at: 826 1045 3054 3554 4530 6238 6320 6697 6931 Size: 219 2009 500 976 1708 82 377 234 Cuts at: 6931 7350 10125 10899 11044 12179 12860 13051 826 Size: 419 2775 774 145 1135 681 191 1471 Fragments arranged by size: 2775 2009 1708 1471 1135 976 774 681 500 419 377 234 219 191 145 82 SfiI GGCCn_nnn'nGGCC Cuts at: 2658 2658 Size: 13696 SfoI GGC'GCC Cuts at: 9014 9993 9014 Size: 979 12717 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 13696 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 13696 SmaI CCC'GGG Cuts at: 1872 3567 10704 11023 1872 Size: 1695 7137 319 4545 Fragments arranged by size: 7137 4545 1695 319 SmlI C'TyrA_G Cuts at: 820 1017 1943 5366 5669 7032 7300 7836 8237 Size: 197 926 3423 303 1363 268 536 401 Cuts at: 8237 9346 11800 12701 12963 820 Size: 1109 2454 901 262 1553 Fragments arranged by size: 3423 2454 1553 1363 1109 926 901 536 401 303 268 262 197 SnaBI TAC'GTA Cuts at: 493 11487 493 Size: 10994 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 13696 SphI G_CATG'C Cuts at: 1132 2392 7237 11054 1132 Size: 1260 4845 3817 3774 Fragments arranged by size: 4845 3817 3774 1260 SspI AAT'ATT Cuts at: 5 52 2050 5293 5654 11620 5 Size: 47 1998 3243 361 5966 2081 Fragments arranged by size: 5966 3243 2081 1998 361 47 StuI AGG'CCT Cuts at: 1517 2073 3003 9036 9344 9468 10317 1517 Size: 556 930 6033 308 124 849 4896 Fragments arranged by size: 6033 4896 930 849 556 308 124 StyI C'CwwG_G Cuts at: 513 1065 2004 2137 2347 2647 2797 3330 3407 Size: 552 939 133 210 300 150 533 77 Cuts at: 3407 3457 3611 3981 3999 5050 5297 6576 7038 Size: 50 154 370 18 1051 247 1279 462 Cuts at: 7038 7073 7482 7650 8553 9031 9105 11135 513 Size: 35 409 168 903 478 74 2030 3074 Fragments arranged by size: 3074 2030 1279 1051 939 903 552 533 478 462 409 370 300 247 210 168 154 150 133 77 74 50 35 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2363 3211 3642 3745 7027 8310 9070 Size: 1255 783 848 431 103 3282 1283 760 Cuts at: 9070 9496 9968 11723 11740 11893 12078 12523 325 Size: 426 472 1755 17 153 185 445 1498 Fragments arranged by size: 3282 1755 1498 1283 1255 848 783 760 472 445 431 426 185 153 103 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5187 6098 6948 Size: 274 80 33 51 1443 3210 911 850 Cuts at: 6948 7239 7419 11942 13654 96 Size: 291 180 4523 1712 138 Fragments arranged by size: 4523 3210 1712 1443 911 850 291 274 180 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3217 3249 3459 3491 4689 4721 7080 7112 9136 Size: 32 210 32 1198 32 2359 32 2024 Cuts at: 9136 9168 13656 13688 3217 Size: 32 4488 32 3225 Fragments arranged by size: 4488 3225 2359 2024 1198 210 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 13696 XbaI T'CTAG_A Cuts at: 3691 8792 11032 3691 Size: 5101 2240 6355 Fragments arranged by size: 6355 5101 2240 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2354 3506 2354 Size: 1152 12544 XhoI C'TCGA_G Cuts at: 1943 7032 7836 1943 Size: 5089 804 7803 Fragments arranged by size: 7803 5089 804 XmaI C'CCGG_G Cuts at: 1870 3565 10702 11021 1870 Size: 1695 7137 319 4545 Fragments arranged by size: 7137 4545 1695 319 XmnI GAAnn'nnTTC Cuts at: 2051 4604 5678 8045 11825 2051 Size: 2553 1074 2367 3780 3922 Fragments arranged by size: 3922 3780 2553 2367 1074 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5570 9600 11504 276 Size: 53 83 186 936 4036 4030 1904 2468 Fragments arranged by size: 4036 4030 2468 1904 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BsiWI BstZ17I FseI FspAI NruI PacI RsrII SrfI SwaI