################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I FbaI FseI FspI HaeII HgaI Hin1I HindIII KasI KpnI Ksp632I KspI MamI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI SwaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccII 14 CGCG CG^CG AocI 195 CCTGAGG CC^T[ACGT]AGG AspHI 532 GAGCAC G[AT]GC[AT]^C AspI 2395 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 2147 TGGCCA TGG^CCA BanI 2097 GGTGCC G^G[CT][GA]CC BanII 79 GAGCCC G[GA]GC[CT]^C BciVI 1605 GGATAC GTATCC(6/5) BcnI 2140 CCCGG CC^[GC]GG BsaI 63 GAGACC GGTCTC(1/5) BsiHKAI 532 GAGCAC G[AT]GC[AT]^C BsmBI 2424 GAGACG CGTCTC(1/5) BstAPI 1845 GCAGGGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 14 CGCG CG^CG Bsu36I 195 CCTGAGG CC^T[ACGT]AGG CfoI 15 GCGC GCG^C DraII 833 AGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 41 CCATGG C^C[GA][CT]GG EaeI 2147 TGGCCA [CT]^GGCC[GA] Eco81I 195 CCTGAGG CC^T[ACGT]AGG EcoNI 2310 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 833 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoRI 859 GAATTC G^AATTC EcoRV 1468 GATATC GAT^ATC EcoT14I 41 CCATGG C^C[AT][AT]GG EcoT22I 117 ATGCAT ATGCA^T FauI 585 GCGGG CCCGC(4/6) HapII 2141 CCGG C^CGG HhaI 15 GCGC GCG^C HinP1I 15 GCGC G^CGC HpaI 1012 GTTAAC GTT^AAC HpaII 2141 CCGG C^CGG MaeII 1609 ACGT A^CGT MaeIII 952 GTGAC ^GT[ACGT]AC MluNI 2147 TGGCCA TGG^CCA MscI 2147 TGGCCA TGG^CCA MspA1I 2232 CCGCTG C[AC]G^C[GT]G MspI 2141 CCGG C^CGG MvnI 14 CGCG CG^CG NciI 2140 CCCGG CC^[GC]GG NcoI 41 CCATGG C^CATGG NsiI 117 ATGCAT ATGCA^T NspI 1850 GCATGC [GA]CATG^[CT] PflFI 2395 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 765 CCATCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SexAI 657 ACCAGGT A^CC[AT]GGT SphI 1850 GCATGC GCATG^C StyI 41 CCATGG C^C[AT][AT]GG Tsp45I 952 GTGAC ^GT[GC]AC Tth111I 2395 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 765 CCATCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AlwI 33 GATCC GGATC(4/5) 1778 GGATC BbsI 2325 GAAGAC GAAGAC(2/6) 1562 GTCTTC BglII 1134 AGATCT A^GATCT 1212 AGATCT BmyI 79 GAGCCC G[AGT]GC[ACT]^C 532 GAGCAC BpuAI 2325 GAAGAC GAAGAC(2/6) 1562 GTCTTC BsmI 1092 GAATGC GAATGC(1/-1) 1290 GCATTC Bsp1286I 79 GAGCCC G[AGT]GC[ACT]^C 532 GAGCAC BspMI 240 GCAGGT ACCTGC(4/8) 2343 GCAGGT BsrDI 2151 CATTGC GCAATG(2/0) 1773 GCAATG BstXI 67 CCAGAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 167 CCAAGAACCTGG BstYI 1134 AGATCT [GA]^GATC[CT] 1212 AGATCT DraI 667 TTTAAA TTT^AAA 717 TTTAAA HincII 1012 GTTAAC GT[CT]^[GA]AC 1517 GTCAAC HindII 1012 GTTAAC GT[CT]^[GA]AC 1517 GTCAAC MfeI 275 CAATTG C^AATTG 2493 CAATTG MflI 1134 AGATCT [GA]^GATC[CT] 1212 AGATCT MslI 1851 CATGCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1899 CATTGGTGTG MunI 275 CAATTG C^AATTG 2493 CAATTG PleI 27 GACTC GAGTC(4/5) 2429 GACTC PstI 238 CTGCAG CTGCA^G 2311 CTGCAG SspI 103 AATATT AAT^ATT 321 AATATT TaqI 890 TCGA T^CGA 1418 TCGA TfiI 558 GATTC G^A[AT]TC 2463 GAATC TthHB8I 890 TCGA T^CGA 1418 TCGA XhoII 1134 AGATCT [GA]^GATC[CT] 1212 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AcsI AfaI AluI AlwNI ApoI AvaII BbvI BfaI BpmI BsaJI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI Tru9I TseI Tsp509I TspRI XbaI