(Circular) (Six-base) MAPSORT of: pFN21AE3330.seq Check: 4391 from: 1 to: 7534 pFN21AE3330 7534 bp With 182 enzymes: SgfI * March 28, 2012 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4120 4543 4792 4120 Size: 423 249 6862 Fragments arranged by size: 6862 423 249 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5344 278 Size: 53 83 186 936 3808 2468 Fragments arranged by size: 3808 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2651 3465 4877 1937 Size: 714 814 1412 4594 Fragments arranged by size: 4594 1412 814 714 Acc65I G'GTAC_C Cuts at: 4855 4855 Size: 7534 AclI AA'CG_TT Cuts at: 1563 5661 6034 1563 Size: 4098 373 3063 Fragments arranged by size: 4098 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2082 2576 2831 3551 3646 4045 5597 6981 Size: 450 494 255 720 95 399 1552 1384 Cuts at: 6981 7249 1632 Size: 268 1917 Fragments arranged by size: 1917 1552 1384 720 494 450 399 268 255 95 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7337 AflIII A'CryG_T Cuts at: 1203 6433 7410 1203 Size: 5230 977 1327 Fragments arranged by size: 5230 1327 977 AgeI A'CCGG_T Cuts at: 3450 6365 3450 Size: 2915 4619 AhdI GACnn_n'nnGTC Cuts at: 4030 6263 4030 Size: 2233 5301 AleI CACnn'nnGTG Cuts at: 1166 4593 1166 Size: 3427 4107 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7534 AlwNI CAG_nnn'CTG Cuts at: 2377 2393 3649 3669 4135 4772 5277 6849 2377 Size: 16 1256 20 466 637 505 1572 3062 Fragments arranged by size: 3062 1572 1256 637 505 466 20 16 ApaI G_GGCC'C Cuts at: 3028 3128 3552 4371 4720 3028 Size: 100 424 819 349 5842 Fragments arranged by size: 5842 819 424 349 100 ApaLI G'TGCA_C Cuts at: 2550 3782 5591 6747 2550 Size: 1232 1809 1156 3337 Fragments arranged by size: 3337 1809 1232 1156 ApoI r'AATT_y Cuts at: 1493 2191 3692 4843 5069 5221 7330 1493 Size: 698 1501 1151 226 152 2109 1697 Fragments arranged by size: 2109 1697 1501 1151 698 226 152 AseI AT'TA_AT Cuts at: 160 1032 6088 160 Size: 872 5056 1606 Fragments arranged by size: 5056 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7534 AvaI C'yCGr_G Cuts at: 1870 1943 2010 2733 3192 3493 4288 4859 1870 Size: 73 67 723 459 301 795 571 4545 Fragments arranged by size: 4545 795 723 571 459 301 73 67 BamHI G'GATC_C Cuts at: 2119 4864 5231 2119 Size: 2745 367 4422 Fragments arranged by size: 4422 2745 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2737 4438 4483 4555 4855 Size: 325 217 627 950 1701 45 72 300 Cuts at: 4855 6310 618 Size: 1455 1842 Fragments arranged by size: 1842 1701 1455 950 627 325 300 217 72 45 BanII G_rGCy'C Cuts at: 1391 2018 2888 3028 3128 3552 3771 4371 4720 Size: 627 870 140 100 424 219 600 349 Cuts at: 4720 4853 1391 Size: 133 4072 Fragments arranged by size: 4072 870 627 600 424 349 219 140 133 100 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2348 2435 4051 928 Size: 971 449 87 1616 4411 Fragments arranged by size: 4411 1616 971 449 87 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5725 5759 1903 Size: 34 3788 34 3678 Fragments arranged by size: 3788 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5426 6636 5426 Size: 1210 6324 BclI T'GATC_A Cuts at: 1548 1548 Size: 7534 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2060 3624 3989 Size: 107 122 71 631 605 388 1564 365 Cuts at: 3989 4496 6145 136 Size: 507 1649 1525 Fragments arranged by size: 1649 1564 1525 631 605 507 388 365 122 107 71 BglII A'GATC_T Cuts at: 3894 7529 3894 Size: 3635 3899 BlpI GC'TnA_GC Cuts at: 4951 4951 Size: 7534 Bme1580I G_kGCm'C Cuts at: 1790 2554 2740 3028 3090 3128 3552 3786 4371 Size: 764 186 288 62 38 424 234 585 Cuts at: 4371 4443 4720 5595 6751 1790 Size: 72 277 875 1156 2573 Fragments arranged by size: 2573 1156 875 764 585 424 288 277 234 186 72 62 38 BmgBI CAC'GTC Cuts at: 1318 2649 3724 1318 Size: 1331 1075 5128 Fragments arranged by size: 5128 1331 1075 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4328 4396 5265 Size: 416 53 83 399 24 2911 68 869 Cuts at: 5265 6223 442 Size: 958 1753 Fragments arranged by size: 2911 1753 958 869 416 399 83 68 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7534 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4774 4774 Size: 7534 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2219 2801 3785 6194 1239 Size: 980 582 984 2409 2579 Fragments arranged by size: 2579 2409 984 980 582 Bpu10I CC'TnA_GC Cuts at: 2962 3072 3650 4322 2962 Size: 110 578 672 6174 Fragments arranged by size: 6174 672 578 110 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5659 6524 6822 5659 Size: 865 298 6371 Fragments arranged by size: 6371 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6197 882 Size: 617 4698 2219 Fragments arranged by size: 4698 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3835 4363 5325 493 Size: 711 2631 528 962 2702 Fragments arranged by size: 2702 2631 962 711 528 BsaBI GATnn'nnATC Cuts at: 2333 7528 2333 Size: 5195 2339 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2448 5341 5723 6358 Size: 53 83 186 936 915 2893 382 635 Cuts at: 6358 275 Size: 1451 Fragments arranged by size: 2893 1451 936 915 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2337 2568 3450 4119 5967 6365 6639 6786 7197 Size: 231 882 669 1848 398 274 147 411 Cuts at: 7197 2337 Size: 2674 Fragments arranged by size: 2674 1848 882 669 411 398 274 231 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2714 2744 1178 Size: 30 165 30 1311 30 5968 Fragments arranged by size: 5968 1311 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3449 3575 4551 4757 4760 4763 1186 Size: 2263 126 976 206 3 3 3957 Fragments arranged by size: 3957 2263 976 206 126 3 3 BseYI C'CCAG_C Cuts at: 2464 2920 2932 3233 3347 3439 3622 3949 4202 Size: 456 12 301 114 92 183 327 253 Cuts at: 4202 4580 4780 6737 7233 2464 Size: 378 200 1957 496 2765 Fragments arranged by size: 2765 1957 496 456 378 327 301 253 200 183 114 92 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2231 2333 4544 4751 1163 Size: 573 495 102 2211 207 3946 Fragments arranged by size: 3946 2211 573 495 207 102 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2036 2055 2067 4984 5745 6773 Size: 275 186 35 19 12 2917 761 1028 Cuts at: 6773 1540 Size: 2301 Fragments arranged by size: 2917 2301 1028 761 275 186 35 19 12 BsiHKAI G_wGCw'C Cuts at: 2554 2675 3592 3786 4328 4727 5595 5680 6751 Size: 121 917 194 542 399 868 85 1071 Cuts at: 6751 2554 Size: 3337 Fragments arranged by size: 3337 1071 917 868 542 399 194 121 85 BsiWI C'GTAC_G Cuts at: 3154 3154 Size: 7534 BsmI GAATG_Cn' Cuts at: 5045 5138 5045 Size: 93 7441 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2554 2675 2740 2888 3028 3090 Size: 399 228 536 121 65 148 140 62 Cuts at: 3090 3128 3552 3592 3771 3786 4328 4371 4443 Size: 38 424 40 179 15 542 43 72 Cuts at: 4443 4720 4727 4853 5595 5680 6751 1391 Size: 277 7 126 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 542 536 424 399 277 228 179 148 140 126 121 85 72 65 62 43 40 38 15 7 BspEI T'CCGG_A Cuts at: 2337 4119 7197 2337 Size: 1782 3078 2674 Fragments arranged by size: 3078 2674 1782 BspHI T'CATG_A Cuts at: 4176 5423 7153 4176 Size: 1247 1730 4557 Fragments arranged by size: 4557 1730 1247 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4120 4512 4543 4792 4889 844 Size: 3276 392 31 249 97 3489 Fragments arranged by size: 3489 3276 392 249 97 31 BsrBI CCG'CTC Cuts at: 1394 1823 2820 2830 5421 1394 Size: 429 997 10 2591 3507 Fragments arranged by size: 3507 2591 997 429 10 BsrDI GCAATG_nn' Cuts at: 65 1248 3868 4348 6029 6203 65 Size: 1183 2620 480 1681 174 1396 Fragments arranged by size: 2620 1681 1396 1183 480 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3450 4125 4206 4236 4480 4486 6178 Size: 110 1640 675 81 30 244 6 1692 Cuts at: 6178 6365 1700 Size: 187 2869 Fragments arranged by size: 2869 1692 1640 675 244 187 110 81 30 6 BsrGI T'GTAC_A Cuts at: 96 3219 96 Size: 3123 4411 BssHII G'CGCG_C Cuts at: 1925 2726 1925 Size: 801 6733 BssSI C'ACGA_G Cuts at: 2510 3181 5594 6606 2510 Size: 671 2413 1012 3438 Fragments arranged by size: 3438 2413 1012 671 BstAPI GCAn_nnn'nTGC Cuts at: 794 4135 4555 794 Size: 3341 420 3773 Fragments arranged by size: 3773 3341 420 BstBI TT'CG_AA Cuts at: 6338 6338 Size: 7534 BstEII G'GTnAC_C Cuts at: 1184 3473 3631 1184 Size: 2289 158 5087 Fragments arranged by size: 5087 2289 158 BstXI CCAn_nnnn'nTGG Cuts at: 2516 2581 2516 Size: 65 7469 BstYI r'GATC_y Cuts at: 1972 2119 3894 4527 4864 5231 5616 5633 7085 Size: 147 1775 633 337 367 385 17 1452 Cuts at: 7085 7171 7183 7529 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1775 1452 633 385 367 346 337 147 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2027 2321 3020 4434 4629 4650 Size: 552 939 23 294 699 1414 195 21 Cuts at: 4650 513 Size: 3397 Fragments arranged by size: 3397 1414 939 699 552 294 195 23 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2797 3223 3241 487 Size: 683 56 1571 426 18 4780 Fragments arranged by size: 4780 1571 683 426 56 18 BtsI GCAGTG_nn' Cuts at: 2248 4606 5050 5844 5864 2248 Size: 2358 444 794 20 3918 Fragments arranged by size: 3918 2358 794 444 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7534 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7499 DraI TTT'AAA Cuts at: 4838 4838 Size: 7534 DraIII CAC_nnn'GTG Cuts at: 2707 3790 3835 2707 Size: 1083 45 6406 Fragments arranged by size: 6406 1083 45 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6541 809 Size: 507 5225 1802 Fragments arranged by size: 5225 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2033 2052 2064 Size: 54 1425 50 275 18 203 19 12 Cuts at: 2064 2412 3516 3852 4981 5870 7499 8 Size: 348 1104 336 1129 889 1629 43 Fragments arranged by size: 1629 1425 1129 1104 889 348 336 275 203 54 50 43 19 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2033 2052 2064 4981 1537 Size: 275 221 19 12 2917 4090 Fragments arranged by size: 4090 2917 275 221 19 12 EarI CTCTTCn'nnn_ Cuts at: 1353 3917 5464 1353 Size: 2564 1547 3423 Fragments arranged by size: 3423 2564 1547 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2341 3748 6121 6495 6641 387 Size: 1064 138 752 1407 2373 374 146 1280 Fragments arranged by size: 2373 1407 1280 1064 752 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2082 2219 2576 2801 2831 3551 3646 Size: 393 450 137 357 225 30 720 95 Cuts at: 3646 3785 4045 5597 6194 6981 7249 1239 Size: 139 260 1552 597 787 268 1524 Fragments arranged by size: 1552 1524 787 720 597 450 393 357 268 260 225 139 137 95 30 EcoNI CCTnn'n_nnAGG Cuts at: 3557 4052 3557 Size: 495 7039 EcoO109I rG'GnC_Cy Cuts at: 2138 2374 3025 3124 3125 3548 3549 3847 4367 Size: 236 651 99 1 423 1 298 520 Cuts at: 4367 4381 4497 4716 2138 Size: 14 116 219 4956 Fragments arranged by size: 4956 651 520 423 298 236 219 116 99 14 1 1 EcoRI G'AATT_C Cuts at: 3692 4843 5221 3692 Size: 1151 378 6005 Fragments arranged by size: 6005 1151 378 FspI TGC'GCA Cuts at: 2909 6040 2909 Size: 3131 4403 FspAI rTGC'GCAy Cuts at: 2909 2909 Size: 7534 HaeII r_GCGC'y Cuts at: 1678 2024 2870 4686 6681 1678 Size: 346 846 1816 1995 2531 Fragments arranged by size: 2531 1995 1816 846 346 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2155 2187 2352 2384 2657 Size: 32 135 32 583 32 165 32 273 Cuts at: 2657 2689 2714 2746 3164 3196 3806 3838 4016 Size: 32 25 32 418 32 610 32 178 Cuts at: 4016 4048 6175 6207 6249 6281 1373 Size: 32 2127 32 42 32 2626 Fragments arranged by size: 2626 2127 610 583 418 273 178 165 135 42 32 32 32 32 32 32 32 32 32 32 32 25 HincII GTy'rAC Cuts at: 669 1657 1938 4468 4878 5124 669 Size: 988 281 2530 410 246 3079 Fragments arranged by size: 3079 2530 988 410 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7534 HpaI GTT'AAC Cuts at: 5124 5124 Size: 7534 KpnI G_GTAC'C Cuts at: 4859 4859 Size: 7534 MfeI C'AATT_G Cuts at: 5133 5133 Size: 7534 MluI A'CGCG_T Cuts at: 7410 7410 Size: 7534 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2192 2495 2643 2847 4119 4287 4665 6648 Size: 887 303 148 204 1272 168 378 1983 Cuts at: 6648 6832 1305 Size: 184 2007 Fragments arranged by size: 2007 1983 1272 887 378 303 204 184 168 148 MscI TGG'CCA Cuts at: 10 64 1489 1832 2414 3518 3854 10 Size: 54 1425 343 582 1104 336 3690 Fragments arranged by size: 3690 1425 1104 582 343 336 54 MslI CAynn'nnrTG Cuts at: 518 1166 2514 2579 2837 3362 4412 4593 4649 Size: 648 1348 65 258 525 1050 181 56 Cuts at: 4649 5492 5851 6010 518 Size: 843 359 159 2042 Fragments arranged by size: 2042 1348 1050 843 648 525 359 258 181 159 65 56 MspA1I CmG'CkG Cuts at: 1606 2029 2038 2323 2627 2851 3325 3334 3975 Size: 423 9 285 304 224 474 9 641 Cuts at: 3975 4825 4950 5627 6775 7020 1606 Size: 850 125 677 1148 245 2120 Fragments arranged by size: 2120 1148 850 677 641 474 423 304 285 245 224 125 9 9 NaeI GCC'GGC Cuts at: 4208 4238 4488 4208 Size: 30 250 7254 Fragments arranged by size: 7254 250 30 NcoI C'CATG_G Cuts at: 513 1065 2004 4434 4650 513 Size: 552 939 2430 216 3397 Fragments arranged by size: 3397 2430 939 552 216 NdeI CA'TA_TG Cuts at: 387 387 Size: 7534 NgoMIV G'CCGG_C Cuts at: 4206 4236 4486 4206 Size: 30 250 7254 Fragments arranged by size: 7254 250 30 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7534 NotI GC'GGCC_GC Cuts at: 2033 2064 4981 2033 Size: 31 2917 4586 Fragments arranged by size: 4586 2917 31 NspI r_CATG'y Cuts at: 1132 1229 2908 3286 4892 6437 1132 Size: 97 1679 378 1606 1545 2229 Fragments arranged by size: 2229 1679 1606 1545 378 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7534 PciI A'CATG_T Cuts at: 6433 6433 Size: 7534 PflMI CCAn_nnn'nTGG Cuts at: 1108 2377 2410 3358 3514 3835 4105 4712 1108 Size: 1269 33 948 156 321 270 607 3930 Fragments arranged by size: 3930 1269 948 607 321 270 156 33 PfoI T'CCnGG_A Cuts at: 2090 2370 2090 Size: 280 7254 PmeI GTTT'AAAC Cuts at: 4838 4838 Size: 7534 PmlI CAC'GTG Cuts at: 3835 3835 Size: 7534 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2504 2536 5603 5635 7116 7148 2504 Size: 32 3067 32 1481 32 2890 Fragments arranged by size: 3067 2890 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2138 2374 3847 2138 Size: 236 1473 5825 Fragments arranged by size: 5825 1473 236 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7534 PsiI TTA'TAA Cuts at: 5104 5104 Size: 7534 PspOMI G'GGCC_C Cuts at: 3024 3124 3548 4367 4716 3024 Size: 100 424 819 349 5842 Fragments arranged by size: 5842 819 424 349 100 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3992 4024 3992 Size: 32 7502 PstI C_TGCA'G Cuts at: 830 3033 4077 4803 4886 830 Size: 2203 1044 726 83 3478 Fragments arranged by size: 3478 2203 1044 726 83 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7534 PvuII CAG'CTG Cuts at: 2851 3334 4825 2851 Size: 483 1491 5560 Fragments arranged by size: 5560 1491 483 SacII CC_GC'GG Cuts at: 2030 2324 2030 Size: 294 7240 SalI G'TCGA_C Cuts at: 1936 4876 1936 Size: 2940 4594 SbfI CC_TGCA'GG Cuts at: 4886 4886 Size: 7534 ScaI AGT'ACT Cuts at: 5782 7494 5782 Size: 1712 5822 SfcI C'TryA_G Cuts at: 826 1045 3029 4073 4799 4882 6017 6698 6889 Size: 219 1984 1044 726 83 1135 681 191 Cuts at: 6889 826 Size: 1471 Fragments arranged by size: 1984 1471 1135 1044 726 681 219 191 83 SfiI GGCCn_nnn'nGGCC Cuts at: 3989 4496 3989 Size: 507 7027 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7534 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7534 SmaI CCC'GGG Cuts at: 1872 2012 2735 3495 4861 1872 Size: 140 723 760 1366 4545 Fragments arranged by size: 4545 1366 760 723 140 SmlI C'TyrA_G Cuts at: 820 1017 1943 5638 6539 6801 820 Size: 197 926 3695 901 262 1553 Fragments arranged by size: 3695 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5325 493 Size: 4832 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7534 SphI G_CATG'C Cuts at: 1132 2908 4892 1132 Size: 1776 1984 3774 Fragments arranged by size: 3774 1984 1776 SspI AAT'ATT Cuts at: 5 52 5458 5 Size: 47 5406 2081 Fragments arranged by size: 5406 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7534 StyI C'CwwG_G Cuts at: 513 1065 2004 2227 3636 4434 4650 4973 513 Size: 552 939 223 1409 798 216 323 3074 Fragments arranged by size: 3074 1409 939 798 552 323 223 216 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2755 4153 5561 5578 5731 5916 6361 Size: 1255 1175 1398 1408 17 153 185 445 Cuts at: 6361 325 Size: 1498 Fragments arranged by size: 1498 1408 1398 1255 1175 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2805 3219 5780 Size: 274 80 33 51 1443 828 414 2561 Cuts at: 5780 7492 96 Size: 1712 138 Fragments arranged by size: 2561 1712 1443 828 414 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2086 2118 3038 3070 7494 7526 2086 Size: 32 920 32 4424 32 2094 Fragments arranged by size: 4424 2094 920 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7534 XbaI T'CTAG_A Cuts at: 4870 4870 Size: 7534 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3355 3832 4102 4598 4648 3355 Size: 477 270 496 50 6241 Fragments arranged by size: 6241 496 477 270 50 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7534 XmaI C'CCGG_G Cuts at: 1870 2010 2733 3493 4859 1870 Size: 140 723 760 1366 4545 Fragments arranged by size: 4545 1366 760 723 140 XmnI GAAnn'nnTTC Cuts at: 4196 5663 4196 Size: 1467 6067 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5342 276 Size: 53 83 186 936 3808 2468 Fragments arranged by size: 3808 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI KasI NarI NruI NsiI PacI PspXI RsrII SacI SanDI SapI SexAI SfoI SrfI SwaI