(Circular) (Six-base) MAPSORT of: pFN21AE2312.seq Check: 96 from: 1 to: 11113 pFN21AE2312 11113 bp With 182 enzymes: SgfI * January 11, 2011 14:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5580 5580 Size: 11113 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8923 278 Size: 53 83 186 936 7387 2468 Fragments arranged by size: 7387 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2652 6021 6171 8456 1937 Size: 715 3369 150 2285 4594 Fragments arranged by size: 4594 3369 2285 715 150 Acc65I G'GTAC_C Cuts at: 8434 8434 Size: 11113 AclI AA'CG_TT Cuts at: 1563 5184 7921 9240 9613 1563 Size: 3621 2737 1319 373 3063 Fragments arranged by size: 3621 3063 2737 1319 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3374 4346 5039 5618 6272 6895 7043 7483 Size: 1742 972 693 579 654 623 148 440 Cuts at: 7483 7904 7979 9176 10560 10828 1632 Size: 421 75 1197 1384 268 1917 Fragments arranged by size: 1917 1742 1384 1197 972 693 654 623 579 440 421 268 148 75 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10916 AflIII A'CryG_T Cuts at: 1203 4000 4224 6039 7986 10012 10989 1203 Size: 2797 224 1815 1947 2026 977 1327 Fragments arranged by size: 2797 2026 1947 1815 1327 977 224 AgeI A'CCGG_T Cuts at: 9944 9944 Size: 11113 AhdI GACnn_n'nnGTC Cuts at: 7357 9842 7357 Size: 2485 8628 AleI CACnn'nnGTG Cuts at: 1166 4499 1166 Size: 3333 7780 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3363 6353 7734 1184 Size: 2179 2990 1381 4563 Fragments arranged by size: 4563 2990 2179 1381 AlwNI CAG_nnn'CTG Cuts at: 3229 4168 4301 4561 5017 7381 8153 8300 8856 Size: 939 133 260 456 2364 772 147 556 Cuts at: 8856 10428 3229 Size: 1572 3914 Fragments arranged by size: 3914 2364 1572 939 772 556 456 260 147 133 ApaI G_GGCC'C Cuts at: 6055 6055 Size: 11113 ApaLI G'TGCA_C Cuts at: 3012 5567 9170 10326 3012 Size: 2555 3603 1156 3799 Fragments arranged by size: 3799 3603 2555 1156 ApoI r'AATT_y Cuts at: 1493 5443 5975 8366 8422 8648 8800 10909 1493 Size: 3950 532 2391 56 226 152 2109 1697 Fragments arranged by size: 3950 2391 2109 1697 532 226 152 56 AseI AT'TA_AT Cuts at: 160 1032 9667 160 Size: 872 8635 1606 Fragments arranged by size: 8635 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11113 AvaI C'yCGr_G Cuts at: 1870 1943 2331 2784 3773 8438 1870 Size: 73 388 453 989 4665 4545 Fragments arranged by size: 4665 4545 989 453 388 73 AvrII C'CTAG_G Cuts at: 7447 7447 Size: 11113 BamHI G'GATC_C Cuts at: 3302 3453 8443 8810 3302 Size: 151 4990 367 5605 Fragments arranged by size: 5605 4990 367 151 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2150 3590 4493 5134 5865 Size: 325 217 627 363 1440 903 641 731 Cuts at: 5865 7435 7587 8303 8434 9889 618 Size: 1570 152 716 131 1455 1842 Fragments arranged by size: 1842 1570 1455 1440 903 731 716 641 627 363 325 217 152 131 BanII G_rGCy'C Cuts at: 1391 4271 6055 6119 6876 7130 7497 8432 1391 Size: 2880 1784 64 757 254 367 935 4072 Fragments arranged by size: 4072 2880 1784 935 757 367 254 64 BbeI G_GCGC'C Cuts at: 7439 7439 Size: 11113 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2652 2891 3536 4582 4934 5047 5525 Size: 971 753 239 645 1046 352 113 478 Cuts at: 5525 6077 7602 928 Size: 552 1525 4439 Fragments arranged by size: 4439 1525 1046 971 753 645 552 478 352 239 113 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2044 2078 2804 2838 5338 5372 7396 Size: 34 107 34 726 34 2500 34 2024 Cuts at: 7396 7430 9304 9338 1903 Size: 34 1874 34 3678 Fragments arranged by size: 3678 2500 2024 1874 726 107 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6991 9005 10215 6991 Size: 2014 1210 7889 Fragments arranged by size: 7889 2014 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 11113 BfrBI ATG'CAT Cuts at: 2798 7201 2798 Size: 4403 6710 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 9724 136 Size: 107 122 71 631 605 8052 1525 Fragments arranged by size: 8052 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3172 4867 11108 3172 Size: 1695 6241 3177 Fragments arranged by size: 6241 3177 1695 BlpI GC'TnA_GC Cuts at: 4270 4302 6875 7811 8530 4270 Size: 32 2573 936 719 6853 Fragments arranged by size: 6853 2573 936 719 32 Bme1580I G_kGCm'C Cuts at: 1790 2153 3016 5320 5571 6055 8308 9174 10330 Size: 363 863 2304 251 484 2253 866 1156 Cuts at: 10330 1790 Size: 2573 Fragments arranged by size: 2573 2304 2253 1156 866 863 484 363 251 BmgBI CAC'GTC Cuts at: 1318 4568 7054 7432 7669 1318 Size: 3250 2486 378 237 4762 Fragments arranged by size: 4762 3250 2486 378 237 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3723 4544 4576 Size: 416 53 83 399 24 2306 821 32 Cuts at: 4576 4964 5163 6997 8844 9802 442 Size: 388 199 1834 1847 958 1753 Fragments arranged by size: 2306 1847 1834 1753 958 821 416 399 388 199 83 53 32 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 11113 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7567 7567 Size: 11113 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2165 5129 5147 6827 7412 8147 9773 1239 Size: 926 2964 18 1680 585 735 1626 2579 Fragments arranged by size: 2964 2579 1680 1626 926 735 585 18 Bpu10I CC'TnA_GC Cuts at: 6916 6916 Size: 11113 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3503 4817 5711 6815 9238 10103 10401 3503 Size: 1314 894 1104 2423 865 298 4215 Fragments arranged by size: 4215 2423 1314 1104 894 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2330 3959 4025 5834 6230 9776 882 Size: 617 831 1629 66 1809 396 3546 2219 Fragments arranged by size: 3546 2219 1809 1629 831 617 396 66 BsaAI yAC'GTr Cuts at: 493 1204 2390 4001 8904 493 Size: 711 1186 1611 4903 2702 Fragments arranged by size: 4903 2702 1611 1186 711 BsaBI GATnn'nnATC Cuts at: 3495 11107 3495 Size: 7612 3501 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6892 7436 8920 9302 Size: 53 83 186 936 5359 544 1484 382 Cuts at: 9302 9937 275 Size: 635 1451 Fragments arranged by size: 5359 1484 1451 936 635 544 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2091 5400 9546 9944 10218 10365 10776 2091 Size: 3309 4146 398 274 147 411 2428 Fragments arranged by size: 4146 3309 2428 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4619 4649 6422 6452 1178 Size: 30 165 30 3216 30 1773 30 5839 Fragments arranged by size: 5839 3216 1773 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2645 4640 5111 5114 5714 6427 8150 1186 Size: 1459 1995 471 3 600 713 1723 4149 Fragments arranged by size: 4149 1995 1723 1459 713 600 471 3 BseYI C'CCAG_C Cuts at: 2485 3422 3524 3822 4561 4762 5844 6259 6514 Size: 937 102 298 739 201 1082 415 255 Cuts at: 6514 6957 8293 10316 10812 2485 Size: 443 1336 2023 496 2786 Fragments arranged by size: 2786 2023 1336 1082 937 739 496 443 415 298 255 201 102 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2633 2701 3668 3751 4455 5018 5690 Size: 573 897 68 967 83 704 563 672 Cuts at: 5690 6102 6359 6563 6763 6765 8141 1163 Size: 412 257 204 200 2 1376 4135 Fragments arranged by size: 4135 1376 967 897 704 672 573 563 412 257 204 200 83 68 2 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 8563 9324 10352 1540 Size: 275 186 6562 761 1028 2301 Fragments arranged by size: 6562 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2067 3016 3514 4543 5571 5754 6119 7497 9174 Size: 949 498 1029 1028 183 365 1378 1677 Cuts at: 9174 9259 10330 2067 Size: 85 1071 2850 Fragments arranged by size: 2850 1677 1378 1071 1029 1028 949 498 365 183 85 BsmI GAATG_Cn' Cuts at: 4744 4794 6108 6429 8624 8717 4744 Size: 50 1314 321 2195 93 7140 Fragments arranged by size: 7140 2195 1314 321 93 50 BsmBI CGTCTCn'nnnn_ Cuts at: 3479 6702 3479 Size: 3223 7890 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2153 3016 3514 4271 4543 5320 Size: 399 277 86 863 498 757 272 777 Cuts at: 5320 5571 5754 6055 6119 6876 7130 7497 8308 Size: 251 183 301 64 757 254 367 811 Cuts at: 8308 8432 9174 9259 10330 1391 Size: 124 742 85 1071 2174 Fragments arranged by size: 2174 1071 863 811 777 757 757 742 498 399 367 301 277 272 254 251 183 124 86 85 64 BspEI T'CCGG_A Cuts at: 5400 10776 5400 Size: 5376 5737 BspHI T'CATG_A Cuts at: 3094 4113 4822 8328 9002 10732 3094 Size: 1019 709 3506 674 1730 3475 Fragments arranged by size: 3506 3475 1730 1019 709 674 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2487 3214 5580 5922 6157 6870 7329 7689 Size: 1643 727 2366 342 235 713 459 360 Cuts at: 7689 8468 844 Size: 779 3489 Fragments arranged by size: 3489 2366 1643 779 727 713 459 360 342 235 BsrBI CCG'CTC Cuts at: 1394 1823 6451 9000 1394 Size: 429 4628 2549 3507 Fragments arranged by size: 4628 3507 2549 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3624 7166 7807 8157 9608 9782 65 Size: 1183 2376 3542 641 350 1451 174 1396 Fragments arranged by size: 3542 2376 1451 1396 1183 641 350 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2235 5271 9757 9944 1700 Size: 110 425 3036 4486 187 2869 Fragments arranged by size: 4486 3036 2869 425 187 110 BsrGI T'GTAC_A Cuts at: 96 4503 6135 96 Size: 4407 1632 5074 Fragments arranged by size: 5074 4407 1632 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 11113 BssSI C'ACGA_G Cuts at: 4391 9173 10185 4391 Size: 4782 1012 5319 Fragments arranged by size: 5319 4782 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 5122 5923 6089 794 Size: 4328 801 166 5818 Fragments arranged by size: 5818 4328 801 166 BstBI TT'CG_AA Cuts at: 9917 9917 Size: 11113 BstEII G'GTnAC_C Cuts at: 1184 7849 1184 Size: 6665 4448 BstXI CCAn_nnnn'nTGG Cuts at: 3601 6502 3601 Size: 2901 8212 BstYI r'GATC_y Cuts at: 1972 3112 3172 3187 3302 3453 4330 4399 4840 Size: 1140 60 15 115 151 877 69 441 Cuts at: 4840 4867 6790 7020 8443 8810 9195 9212 10664 Size: 27 1923 230 1423 367 385 17 1452 Cuts at: 10664 10750 10762 11108 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1923 1452 1423 1140 877 441 385 367 346 230 151 115 86 69 60 27 17 15 12 BstZ17I GTA'TAC Cuts at: 2653 6022 6172 2653 Size: 3369 150 7594 Fragments arranged by size: 7594 3369 150 Bsu36I CC'TnA_GG Cuts at: 2743 5412 7607 2743 Size: 2669 2195 6249 Fragments arranged by size: 6249 2669 2195 BtgI C'CryG_G Cuts at: 513 1065 2004 3183 3218 6057 6147 6627 6882 Size: 552 939 1179 35 2839 90 480 255 Cuts at: 6882 513 Size: 4744 Fragments arranged by size: 4744 2839 1179 939 552 480 255 90 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 6331 487 Size: 683 56 5105 5269 Fragments arranged by size: 5269 5105 683 56 BtsI GCAGTG_nn' Cuts at: 4313 6529 6717 6775 8101 8629 9423 9443 4313 Size: 2216 188 58 1326 528 794 20 5983 Fragments arranged by size: 5983 2216 1326 794 528 188 58 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 11113 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 7844 7879 529 Size: 35 7280 35 3763 Fragments arranged by size: 7280 3763 35 35 DraI TTT'AAA Cuts at: 8417 8417 Size: 11113 DraIII CAC_nnn'GTG Cuts at: 3020 3020 Size: 11113 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2705 3951 10120 809 Size: 507 1389 1246 6169 1802 Fragments arranged by size: 6169 1802 1389 1246 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2889 3006 3273 Size: 54 1425 50 275 18 1059 117 267 Cuts at: 3273 3681 3900 5269 6630 8560 9449 11078 8 Size: 408 219 1369 1361 1930 889 1629 43 Fragments arranged by size: 1930 1629 1425 1369 1361 1059 889 408 275 267 219 117 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 8560 1537 Size: 275 6748 4090 Fragments arranged by size: 6748 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2200 2664 3327 3732 6359 6484 6914 7502 Size: 847 464 663 405 2627 125 430 588 Cuts at: 7502 8174 8181 9043 1353 Size: 672 7 862 3423 Fragments arranged by size: 3423 2627 862 847 672 663 588 464 430 405 125 7 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 9700 10074 10220 387 Size: 1064 138 435 7676 374 146 1280 Fragments arranged by size: 7676 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 6117 7495 6117 Size: 1378 9735 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2165 3374 4346 5039 5129 5147 5618 Size: 393 533 1209 972 693 90 18 471 Cuts at: 5618 6272 6827 6895 7043 7412 7483 7904 7979 Size: 654 555 68 148 369 71 421 75 Cuts at: 7979 8147 9176 9773 10560 10828 1239 Size: 168 1029 597 787 268 1524 Fragments arranged by size: 1524 1209 1029 972 787 693 654 597 555 533 471 421 393 369 268 168 148 90 75 71 68 18 EcoNI CCTnn'n_nnAGG Cuts at: 4235 6866 7754 4235 Size: 2631 888 7594 Fragments arranged by size: 7594 2631 888 EcoO109I rG'GnC_Cy Cuts at: 3132 3241 3878 6051 6052 6154 7627 8150 3132 Size: 109 637 2173 1 102 1473 523 6095 Fragments arranged by size: 6095 2173 1473 637 523 109 102 1 EcoRI G'AATT_C Cuts at: 8422 8800 8422 Size: 378 10735 EcoRV GAT'ATC Cuts at: 2395 2395 Size: 11113 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4039 7159 7171 8299 4039 Size: 3120 12 1128 6853 Fragments arranged by size: 6853 3120 1128 12 FspI TGC'GCA Cuts at: 9619 9619 Size: 11113 HaeII r_GCGC'y Cuts at: 1678 3679 4861 6130 7108 7439 10260 1678 Size: 2001 1182 1269 978 331 2821 2531 Fragments arranged by size: 2821 2531 2001 1269 1182 978 331 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2111 2143 3116 3148 3867 Size: 32 135 32 539 32 973 32 719 Cuts at: 3867 3899 7343 7375 7524 7556 7715 7747 8204 Size: 32 3444 32 149 32 159 32 457 Cuts at: 8204 8236 9754 9786 9828 9860 1373 Size: 32 1518 32 42 32 2626 Fragments arranged by size: 3444 2626 1518 973 719 539 457 159 149 135 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4006 4381 8457 8703 669 Size: 988 281 2068 375 4076 246 3079 Fragments arranged by size: 4076 3079 2068 988 375 281 246 HindIII A'AGCT_T Cuts at: 748 3891 4691 748 Size: 3143 800 7170 Fragments arranged by size: 7170 3143 800 HpaI GTT'AAC Cuts at: 8703 8703 Size: 11113 KasI G'GCGC_C Cuts at: 7435 7435 Size: 11113 KpnI G_GTAC'C Cuts at: 8438 8438 Size: 11113 MfeI C'AATT_G Cuts at: 8712 8712 Size: 11113 MluI A'CGCG_T Cuts at: 7986 10989 7986 Size: 3003 8110 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2256 3967 4505 5535 5921 6781 7329 8259 Size: 951 1711 538 1030 386 860 548 930 Cuts at: 8259 10227 10411 1305 Size: 1968 184 2007 Fragments arranged by size: 2007 1968 1711 1030 951 930 860 548 538 386 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3008 3275 3683 3902 6632 Size: 54 1425 343 1176 267 408 219 2730 Cuts at: 6632 10 Size: 4491 Fragments arranged by size: 4491 2730 1425 1176 408 343 267 219 54 MslI CAynn'nnrTG Cuts at: 518 1166 2156 2960 3581 3599 3923 4499 5087 Size: 648 990 804 621 18 324 576 588 Cuts at: 5087 5532 5810 6146 6552 6968 7029 7212 7964 Size: 445 278 336 406 416 61 183 752 Cuts at: 7964 9071 9430 9589 518 Size: 1107 359 159 2042 Fragments arranged by size: 2042 1107 990 804 752 648 621 588 576 445 416 406 359 336 324 278 183 159 61 18 MspA1I CmG'CkG Cuts at: 1606 2411 2812 2920 3727 3826 4883 5014 5212 Size: 805 401 108 807 99 1057 131 198 Cuts at: 5212 5788 5826 5848 6066 7714 7891 7999 8125 Size: 576 38 22 218 1648 177 108 126 Cuts at: 8125 8529 9206 10354 10599 1606 Size: 404 677 1148 245 2120 Fragments arranged by size: 2120 1648 1148 1057 807 805 677 576 404 401 245 218 198 177 131 126 108 108 99 38 22 NarI GG'CG_CC Cuts at: 7436 7436 Size: 11113 NcoI C'CATG_G Cuts at: 513 1065 2004 3183 6147 6627 6882 513 Size: 552 939 1179 2964 480 255 4744 Fragments arranged by size: 4744 2964 1179 939 552 480 255 NdeI CA'TA_TG Cuts at: 387 4069 387 Size: 3682 7431 NheI G'CTAG_C Cuts at: 1052 1052 Size: 11113 NotI GC'GGCC_GC Cuts at: 8560 8560 Size: 11113 NsiI A_TGCA'T Cuts at: 2800 7203 2800 Size: 4403 6710 NspI r_CATG'y Cuts at: 1132 1229 3344 3556 4151 4228 4705 5371 5506 Size: 97 2115 212 595 77 477 666 135 Cuts at: 5506 7507 7711 8471 10016 1132 Size: 2001 204 760 1545 2229 Fragments arranged by size: 2229 2115 2001 1545 760 666 595 477 212 204 135 97 77 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 11113 PciI A'CATG_T Cuts at: 4224 10012 4224 Size: 5788 5325 PflMI CCAn_nnn'nTGG Cuts at: 1108 2575 3271 6514 6934 8300 1108 Size: 1467 696 3243 420 1366 3921 Fragments arranged by size: 3921 3243 1467 1366 696 420 PfoI T'CCnGG_A Cuts at: 3134 4184 4218 6195 3134 Size: 1050 34 1977 8052 Fragments arranged by size: 8052 1977 1050 34 PmeI GTTT'AAAC Cuts at: 8417 8417 Size: 11113 PmlI CAC'GTG Cuts at: 2390 2390 Size: 11113 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 9182 9214 10695 10727 9182 Size: 32 1481 32 9568 Fragments arranged by size: 9568 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3132 3241 3878 7627 3132 Size: 109 637 3749 6618 Fragments arranged by size: 6618 3749 637 109 PshAI GACnn'nnGTC Cuts at: 1613 3876 1613 Size: 2263 8850 PsiI TTA'TAA Cuts at: 8683 8683 Size: 11113 PspOMI G'GGCC_C Cuts at: 6051 6051 Size: 11113 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 6176 6208 7295 7327 6176 Size: 32 1087 32 9962 Fragments arranged by size: 9962 1087 32 32 PstI C_TGCA'G Cuts at: 830 2811 4557 5118 5577 5919 6114 6524 7326 Size: 1981 1746 561 459 342 195 410 802 Cuts at: 7326 7692 8465 830 Size: 366 773 3478 Fragments arranged by size: 3478 1981 1746 802 773 561 459 410 366 342 195 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11113 PvuII CAG'CTG Cuts at: 2411 2920 3727 3826 4883 5014 5212 5848 7714 Size: 509 807 99 1057 131 198 636 1866 Cuts at: 7714 7891 7999 8125 2411 Size: 177 108 126 5399 Fragments arranged by size: 5399 1866 1057 807 636 509 198 177 131 126 108 99 SacI G_AGCT'C Cuts at: 6119 7497 6119 Size: 1378 9735 SalI G'TCGA_C Cuts at: 1936 8455 1936 Size: 6519 4594 SapI GCTCTTCn'nnn_ Cuts at: 2200 6484 6914 7502 2200 Size: 4284 430 588 5811 Fragments arranged by size: 5811 4284 588 430 SbfI CC_TGCA'GG Cuts at: 7326 7692 8465 7326 Size: 366 773 9974 Fragments arranged by size: 9974 773 366 ScaI AGT'ACT Cuts at: 4637 5801 7007 9361 11073 4637 Size: 1164 1206 2354 1712 4677 Fragments arranged by size: 4677 2354 1712 1206 1164 SexAI A'CCwGG_T Cuts at: 3243 6981 3243 Size: 3738 7375 SfcI C'TryA_G Cuts at: 826 1045 2807 4553 4631 5114 5573 5915 6110 Size: 219 1762 1746 78 483 459 342 195 Cuts at: 6110 6520 7322 7688 8461 9596 10277 10468 826 Size: 410 802 366 773 1135 681 191 1471 Fragments arranged by size: 1762 1746 1471 1135 802 773 681 483 459 410 366 342 219 195 191 78 SfoI GGC'GCC Cuts at: 7437 7437 Size: 11113 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11113 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11113 SmaI CCC'GGG Cuts at: 1872 8440 1872 Size: 6568 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3482 4832 5726 6830 9217 10118 Size: 197 926 1539 1350 894 1104 2387 901 Cuts at: 10118 10380 820 Size: 262 1553 Fragments arranged by size: 2387 1553 1539 1350 1104 926 901 894 262 197 SnaBI TAC'GTA Cuts at: 493 8904 493 Size: 8411 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 11113 SphI G_CATG'C Cuts at: 1132 3556 5506 7711 8471 1132 Size: 2424 1950 2205 760 3774 Fragments arranged by size: 3774 2424 2205 1950 760 SspI AAT'ATT Cuts at: 5 52 9037 5 Size: 47 8985 2081 Fragments arranged by size: 8985 2081 47 StuI AGG'CCT Cuts at: 1517 7778 1517 Size: 6261 4852 StyI C'CwwG_G Cuts at: 513 1065 2004 3183 3256 3642 4014 4593 4743 Size: 552 939 1179 73 386 372 579 150 Cuts at: 4743 6147 6495 6570 6627 6882 7447 7524 7869 Size: 1404 348 75 57 255 565 77 345 Cuts at: 7869 8268 8552 513 Size: 399 284 3074 Fragments arranged by size: 3074 1404 1179 939 579 565 552 399 386 372 348 345 284 255 150 77 75 73 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3836 4398 4511 7794 7868 8188 9140 Size: 1255 2256 562 113 3283 74 320 952 Cuts at: 9140 9157 9310 9495 9940 325 Size: 17 153 185 445 1498 Fragments arranged by size: 3283 2256 1498 1255 952 562 445 320 185 153 113 74 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2040 4503 4635 Size: 274 80 33 51 1443 63 2463 132 Cuts at: 4635 5799 6135 7005 8322 8361 9359 11071 96 Size: 1164 336 870 1317 39 998 1712 138 Fragments arranged by size: 2463 1712 1443 1317 1164 998 870 336 274 138 132 80 63 51 39 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2379 2411 11073 11105 2379 Size: 32 8662 32 2387 Fragments arranged by size: 8662 2387 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 11113 XbaI T'CTAG_A Cuts at: 8449 8449 Size: 11113 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2372 3640 5599 5628 6154 6463 7010 8275 2372 Size: 1268 1959 29 526 309 547 1265 5210 Fragments arranged by size: 5210 1959 1268 1265 547 526 309 29 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 11113 XmaI C'CCGG_G Cuts at: 1870 8438 1870 Size: 6568 4545 XmnI GAAnn'nnTTC Cuts at: 5444 7139 9242 5444 Size: 1695 2103 7315 Fragments arranged by size: 7315 2103 1695 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8921 276 Size: 53 83 186 936 7387 2468 Fragments arranged by size: 7387 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbvCI BsiWI FseI FspAI NaeI NgoMIV NruI PacI PspXI RsrII SacII SanDI SfiI SrfI SwaI