Restriction map for
pFN21AE0798
(Circular) (Six-base) MAPSORT of: pFN21AE0798.seq Check: 3556 from: 1 to: 9553
pFN21AE0798 9553 bp
With 182 enzymes: SgfI *
September 6, 2010 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2610 4678 2610
Size: 2068 7485
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4098 6349 6498 7363
Size: 53 83 186 936 2562 2251 149 865
Cuts at: 7363 278
Size: 2468
Fragments arranged by size:
2562 2468 2251 936 865 186 149 83
53
AccI GT'mk_AC
Cuts at: 1937 6896 1937
Size: 4959 4594
Acc65I G'GTAC_C
Cuts at: 2193 3615 6874 2193
Size: 1422 3259 4872
Fragments arranged by size:
4872 3259 1422
AclI AA'CG_TT
Cuts at: 1563 7680 8053 1563
Size: 6117 373 3063
Fragments arranged by size:
6117 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2880 3621 6101 6758 7616 9000 9268 1632
Size: 1248 741 2480 657 858 1384 268 1917
Fragments arranged by size:
2480 1917 1384 1248 858 741 657 268
AfeI AGC'GCT
Cuts at: 3291 4245 3291
Size: 954 8599
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9356
AflIII A'CryG_T
Cuts at: 1203 5001 5154 8452 9429 1203
Size: 3798 153 3298 977 1327
Fragments arranged by size:
3798 3298 1327 977 153
AgeI A'CCGG_T
Cuts at: 8384 8384
Size: 9553
AhdI GACnn_n'nnGTC
Cuts at: 4237 5713 8282 4237
Size: 1476 2569 5508
Fragments arranged by size:
5508 2569 1476
AleI CACnn'nnGTG
Cuts at: 1166 6587 1166
Size: 5421 4132
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3738 4760 1184
Size: 2554 1022 5977
Fragments arranged by size:
5977 2554 1022
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5987 6019 5987
Size: 32 9521
AlwNI CAG_nnn'CTG
Cuts at: 2908 3343 3502 5513 5535 5554 7296 8868 2908
Size: 435 159 2011 22 19 1742 1572 3593
Fragments arranged by size:
3593 2011 1742 1572 435 159 22 19
ApaI G_GGCC'C
Cuts at: 2063 2093 2139 2328 2433 2510 2778 4419 4483
Size: 30 46 189 105 77 268 1641 64
Cuts at: 4483 2063
Size: 7133
Fragments arranged by size:
7133 1641 268 189 105 77 64 46
30
ApaLI G'TGCA_C
Cuts at: 7610 8766 7610
Size: 1156 8397
ApoI r'AATT_y
Cuts at: 1493 3147 5246 5265 6862 7088 7240 9349 1493
Size: 1654 2099 19 1597 226 152 2109 1697
Fragments arranged by size:
2109 2099 1697 1654 1597 226 152 19
AscI GG'CGCG_CC
Cuts at: 5734 5734
Size: 9553
AseI AT'TA_AT
Cuts at: 160 1032 8107 160
Size: 872 7075 1606
Fragments arranged by size:
7075 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9553
AvaI C'yCGr_G
Cuts at: 1870 1943 2056 2098 2636 2722 2800 2813 3002
Size: 73 113 42 538 86 78 13 189
Cuts at: 3002 3170 3932 3943 4760 5239 5472 6395 6771
Size: 168 762 11 817 479 233 923 376
Cuts at: 6771 6878 1870
Size: 107 4545
Fragments arranged by size:
4545 923 817 762 538 479 376 233
189 168 113 107 86 78 73 42
13 11
BamHI G'GATC_C
Cuts at: 4511 6883 7250 4511
Size: 2372 367 6814
Fragments arranged by size:
6814 2372 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2193 2456 2737 3031 3615
Size: 325 217 627 406 263 281 294 584
Cuts at: 3615 4711 5096 5791 6874 8329 618
Size: 1096 385 695 1083 1455 1842
Fragments arranged by size:
1842 1455 1096 1083 695 627 584 406
385 325 294 281 263 217
BanII G_rGCy'C
Cuts at: 1391 2063 2093 2117 2139 2328 2433 2510 2551
Size: 672 30 24 22 189 105 77 41
Cuts at: 2551 2778 3216 4419 4483 4767 5439 5487 5575
Size: 227 438 1203 64 284 672 48 88
Cuts at: 5575 5934 6411 6542 6872 1391
Size: 359 477 131 330 4072
Fragments arranged by size:
4072 1203 672 672 477 438 359 330
284 227 189 131 105 88 77 64
48 41 30 24 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2415 2433 4364 5790 6355 6561 6571
Size: 971 516 18 1931 1426 565 206 10
Cuts at: 6571 6855 928
Size: 284 3626
Fragments arranged by size:
3626 1931 1426 971 565 516 284 206
18 10
BbvCI CC'TCA_GC
Cuts at: 2155 2155
Size: 9553
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3610 3644 4801 4835 4988 5022 7744
Size: 34 1673 34 1157 34 153 34 2722
Cuts at: 7744 7778 1903
Size: 34 3678
Fragments arranged by size:
3678 2722 1673 1157 153 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7445 8655 7445
Size: 1210 8343
BclI T'GATC_A
Cuts at: 1548 5517 6321 1548
Size: 3969 804 4780
Fragments arranged by size:
4780 3969 804
BfrBI ATG'CAT
Cuts at: 2242 2242
Size: 9553
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8164 136
Size: 107 122 71 631 605 6492 1525
Fragments arranged by size:
6492 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5136 9548 5136
Size: 4412 5141
BlpI GC'TnA_GC
Cuts at: 5465 6353 6970 5465
Size: 888 617 8048
Fragments arranged by size:
8048 888 617
Bme1580I G_kGCm'C
Cuts at: 1790 2063 2093 2139 2328 2433 2461 2510 2778
Size: 273 30 46 189 105 28 49 268
Cuts at: 2778 2811 4419 4483 4739 5796 6101 7614 8770
Size: 33 1608 64 256 1057 305 1513 1156
Cuts at: 8770 1790
Size: 2573
Fragments arranged by size:
2573 1608 1513 1156 1057 305 273 268
256 189 105 64 49 46 33 30
28
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9553
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2989 4465 4957
Size: 416 53 83 399 24 1572 1476 492
Cuts at: 4957 7284 8242 442
Size: 2327 958 1753
Fragments arranged by size:
2327 1753 1572 1476 958 492 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9553
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2410 2916 5458 5953 6283 6682 6844 2410
Size: 506 2542 495 330 399 162 5119
Fragments arranged by size:
5119 2542 506 495 399 330 162
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1998 2097 2208 2856 3314 3392 3428 3941
Size: 759 99 111 648 458 78 36 513
Cuts at: 3941 5483 5704 5777 6125 6554 8213 1239
Size: 1542 221 73 348 429 1659 2579
Fragments arranged by size:
2579 1659 1542 759 648 513 458 429
348 221 111 99 78 73 36
Bpu10I CC'TnA_GC
Cuts at: 2155 3312 6829 2155
Size: 1157 3517 4879
Fragments arranged by size:
4879 3517 1157
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3200 4928 6056 7678 8543 8841 3200
Size: 1728 1128 1622 865 298 3912
Fragments arranged by size:
3912 1728 1622 1128 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3821 5798 8216 882
Size: 617 2322 1977 2418 2219
Fragments arranged by size:
2418 2322 2219 1977 617
BsaAI yAC'GTr
Cuts at: 493 1204 3522 3685 3868 4181 4386 7344 493
Size: 711 2318 163 183 313 205 2958 2702
Fragments arranged by size:
2958 2702 2318 711 313 205 183 163
BsaBI GATnn'nnATC
Cuts at: 9547 9547
Size: 9553
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3791 4095 4903 6168
Size: 53 83 186 936 2258 304 808 1265
Cuts at: 6168 6346 6495 7360 7742 8377 275
Size: 178 149 865 382 635 1451
Fragments arranged by size:
2258 1451 1265 936 865 808 635 382
304 186 178 149 83 53
BsaWI w'CCGG_w
Cuts at: 4548 7986 8384 8658 8805 9216 4548
Size: 3438 398 274 147 411 4885
Fragments arranged by size:
4885 3438 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2336 2366 2650 2680 3101
Size: 30 165 30 933 30 284 30 421
Cuts at: 3101 3131 5987 6017 6050 6080 6158 6188 1178
Size: 30 2856 30 33 30 78 30 4543
Fragments arranged by size:
4543 2856 933 421 284 165 78 33
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2028 3623 4019 4022 4025 4073 4892 5186
Size: 842 1595 396 3 3 48 819 294
Cuts at: 5186 5651 5780 5792 5849 5852 5897 5936 5999
Size: 465 129 12 57 3 45 39 63
Cuts at: 5999 6002 6005 6008 6107 6122 6134 6166 6644
Size: 3 3 3 99 15 12 32 478
Cuts at: 6644 6653 6656 6671 6821 6833 1186
Size: 9 3 15 150 12 3906
Fragments arranged by size:
3906 1595 842 819 478 465 396 294
150 129 99 63 57 48 45 39
32 15 15 12 12 12 9 3
3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2086 2138 2315 2333 3015 4516 4542 5477 8756
Size: 52 177 18 682 1501 26 935 3279
Cuts at: 8756 9252 2086
Size: 496 2387
Fragments arranged by size:
3279 2387 1501 935 682 496 177 52
26 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2519 2636 3023 5602 1163
Size: 573 783 117 387 2579 5114
Fragments arranged by size:
5114 2579 783 573 387 117
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2630 6010 6251 6748 6758 6786
Size: 275 186 629 3380 241 497 10 28
Cuts at: 6786 7003 7764 8792 1540
Size: 217 761 1028 2301
Fragments arranged by size:
3380 2301 1028 761 629 497 275 241
217 186 28 10
BsiHKAI G_wGCw'C
Cuts at: 2551 2897 3216 4401 4695 4767 5025 5190 5610
Size: 346 319 1185 294 72 258 165 420
Cuts at: 5610 5763 5934 6411 7614 7699 8770 2551
Size: 153 171 477 1203 85 1071 3334
Fragments arranged by size:
3334 1203 1185 1071 477 420 346 319
294 258 171 165 153 85 72
BsiWI C'GTAC_G
Cuts at: 4461 4581 4461
Size: 120 9433
BsmI GAATG_Cn'
Cuts at: 2881 3438 4135 6450 7064 7157 2881
Size: 557 697 2315 614 93 5277
Fragments arranged by size:
5277 2315 697 614 557 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4102 4910 4102
Size: 808 8745
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 2093 2117 2139 2328 2433 2461
Size: 399 273 30 24 22 189 105 28
Cuts at: 2461 2510 2551 2778 2811 2897 3216 4401 4419
Size: 49 41 227 33 86 319 1185 18
Cuts at: 4419 4483 4695 4739 4767 5025 5190 5439 5487
Size: 64 212 44 28 258 165 249 48
Cuts at: 5487 5575 5610 5763 5796 5934 6101 6411 6542
Size: 88 35 153 33 138 167 310 131
Cuts at: 6542 6872 7614 7699 8770 1391
Size: 330 742 85 1071 2174
Fragments arranged by size:
2174 1185 1071 742 399 330 319 310
273 258 249 227 212 189 167 165
153 138 131 105 88 86 85 64
49 48 44 41 35 33 33 30
28 28 24 22 18
BspEI T'CCGG_A
Cuts at: 4548 9216 4548
Size: 4668 4885
BspHI T'CATG_A
Cuts at: 5328 7442 9172 5328
Size: 2114 1730 5709
Fragments arranged by size:
5709 2114 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2610 4678 5562 6908 844
Size: 1766 2068 884 1346 3489
Fragments arranged by size:
3489 2068 1766 1346 884
BsrBI CCG'CTC
Cuts at: 1394 1823 3541 3565 5731 5971 6828 7440 1394
Size: 429 1718 24 2166 240 857 612 3507
Fragments arranged by size:
3507 2166 1718 857 612 429 240 24
BsrDI GCAATG_nn'
Cuts at: 65 1248 8048 8222 65
Size: 1183 6800 174 1396
Fragments arranged by size:
6800 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3010 3408 5696 6170 6732 6747 6781
Size: 110 1200 398 2288 474 562 15 34
Cuts at: 6781 8197 8384 1700
Size: 1416 187 2869
Fragments arranged by size:
2869 2288 1416 1200 562 474 398 187
110 34 15
BsrGI T'GTAC_A
Cuts at: 96 4308 96
Size: 4212 5341
BssHII G'CGCG_C
Cuts at: 1925 5734 1925
Size: 3809 5744
BssSI C'ACGA_G
Cuts at: 2890 5762 5831 5938 7613 8625 2890
Size: 2872 69 107 1675 1012 3818
Fragments arranged by size:
3818 2872 1675 1012 107 69
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2218 2653 3776 4844 5020 794
Size: 1424 435 1123 1068 176 5327
Fragments arranged by size:
5327 1424 1123 1068 435 176
BstBI TT'CG_AA
Cuts at: 8357 8357
Size: 9553
BstEII G'GTnAC_C
Cuts at: 1184 2360 4652 1184
Size: 1176 2292 6085
Fragments arranged by size:
6085 2292 1176
BstXI CCAn_nnnn'nTGG
Cuts at: 2186 2341 2834 3514 2186
Size: 155 493 680 8225
Fragments arranged by size:
8225 680 493 155
BstYI r'GATC_y
Cuts at: 1972 3099 3476 3882 4078 4511 4620 5136 5643
Size: 1127 377 406 196 433 109 516 507
Cuts at: 5643 6883 7250 7635 7652 9104 9190 9202 9548
Size: 1240 367 385 17 1452 86 12 346
Cuts at: 9548 1972
Size: 1977
Fragments arranged by size:
1977 1452 1240 1127 516 507 433 406
385 377 367 346 196 109 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2199 3224 5375 2199
Size: 1025 2151 6377
Fragments arranged by size:
6377 2151 1025
BtgI C'CryG_G
Cuts at: 513 1065 2229 2340 2868 3027 3077 4053 4203
Size: 552 1164 111 528 159 50 976 150
Cuts at: 4203 4488 6010 6423 513
Size: 285 1522 413 3643
Fragments arranged by size:
3643 1522 1164 976 552 528 413 285
159 150 111 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3121 4104 4357 487
Size: 683 56 1895 983 253 5683
Fragments arranged by size:
5683 1895 983 683 253 56
BtsI GCAGTG_nn'
Cuts at: 3035 3519 4672 4832 6071 6346 7069 7863 7883
Size: 484 1153 160 1239 275 723 794 20
Cuts at: 7883 3035
Size: 4705
Fragments arranged by size:
4705 1239 1153 794 723 484 275 160
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9553
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9518
DraI TTT'AAA
Cuts at: 5368 6857 5368
Size: 1489 8064
DraIII CAC_nnn'GTG
Cuts at: 4708 4915 5299 4708
Size: 207 384 8962
Fragments arranged by size:
8962 384 207
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5807 6563 8560 809
Size: 507 4491 756 1997 1802
Fragments arranged by size:
4491 1997 1802 756 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2042 2574 2627
Size: 54 1425 50 275 18 212 532 53
Cuts at: 2627 3180 3516 4335 6007 6248 6709 6745 6755
Size: 553 336 819 1672 241 461 36 10
Cuts at: 6755 7000 7889 9518 8
Size: 245 889 1629 43
Fragments arranged by size:
1672 1629 1425 889 819 553 532 461
336 275 245 241 212 54 53 50
43 36 18 10
EagI C'GGCC_G
Cuts at: 1537 1812 2627 6007 6248 6745 6755 7000 1537
Size: 275 815 3380 241 497 10 245 4090
Fragments arranged by size:
4090 3380 815 497 275 245 241 10
EarI CTCTTCn'nnn_
Cuts at: 1353 3990 3996 4011 6111 6642 7483 1353
Size: 2637 6 15 2100 531 841 3423
Fragments arranged by size:
3423 2637 2100 841 531 15 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2046 2107 5414 5600 8140 8514
Size: 1064 138 457 61 3307 186 2540 374
Cuts at: 8514 8660 387
Size: 146 1280
Fragments arranged by size:
3307 2540 1280 1064 457 374 186 146
138 61
EcoICRI GAG'CTC
Cuts at: 2549 3214 4765 5932 6409 2549
Size: 665 1551 1167 477 5693
Fragments arranged by size:
5693 1551 1167 665 477
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1998 2097 2208 2856 2880 3314 3392
Size: 393 366 99 111 648 24 434 78
Cuts at: 3392 3428 3621 3941 5483 5704 5777 6101 6125
Size: 36 193 320 1542 221 73 324 24
Cuts at: 6125 6554 6758 7616 8213 9000 9268 1239
Size: 429 204 858 597 787 268 1524
Fragments arranged by size:
1542 1524 858 787 648 597 434 429
393 366 324 320 268 221 204 193
111 99 78 73 36 24 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2043 2790 2835 3347 4874 5918 6488 2043
Size: 747 45 512 1527 1044 570 5108
Fragments arranged by size:
5108 1527 1044 747 570 512 45
EcoO109I rG'GnC_Cy
Cuts at: 2048 2060 2090 2102 2135 2183 2324 2325 2429
Size: 12 30 12 33 48 141 1 104
Cuts at: 2429 2430 2506 2690 2726 2774 2795 2840 2864
Size: 1 76 184 36 48 21 45 24
Cuts at: 2864 3082 3174 3220 4171 4416 4480 5551 6768
Size: 218 92 46 951 245 64 1071 1217
Cuts at: 6768 2048
Size: 4833
Fragments arranged by size:
4833 1217 1071 951 245 218 184 141
104 92 76 64 48 48 46 45
36 33 30 24 21 12 12 1
1
EcoRI G'AATT_C
Cuts at: 3147 5265 6862 7240 3147
Size: 2118 1597 378 5460
Fragments arranged by size:
5460 2118 1597 378
FspI TGC'GCA
Cuts at: 5858 8059 5858
Size: 2201 7352
FspAI rTGC'GCAy
Cuts at: 5858 5858
Size: 9553
HaeII r_GCGC'y
Cuts at: 1678 3293 3337 3409 3652 4247 4775 8700 1678
Size: 1615 44 72 243 595 528 3925 2531
Fragments arranged by size:
3925 2531 1615 595 528 243 72 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2154 2186 2336 2368 2538
Size: 32 135 32 582 32 150 32 170
Cuts at: 2538 2570 4063 4082 4095 4114 4223 4255 5699
Size: 32 1493 19 13 19 109 32 1444
Cuts at: 5699 5731 6158 6190 6336 6368 8194 8226 8268
Size: 32 427 32 146 32 1826 32 42
Cuts at: 8268 8300 1373
Size: 32 2626
Fragments arranged by size:
2626 1826 1493 1444 582 427 170 150
146 135 109 42 32 32 32 32
32 32 32 32 32 32 32 19
19 13
HincII GTy'rAC
Cuts at: 669 1657 1938 5626 5812 6897 7143 669
Size: 988 281 3688 186 1085 246 3079
Fragments arranged by size:
3688 3079 1085 988 281 246 186
HindIII A'AGCT_T
Cuts at: 748 4754 6704 748
Size: 4006 1950 3597
Fragments arranged by size:
4006 3597 1950
HpaI GTT'AAC
Cuts at: 7143 7143
Size: 9553
KpnI G_GTAC'C
Cuts at: 2197 3619 6878 2197
Size: 1422 3259 4872
Fragments arranged by size:
4872 3259 1422
MfeI C'AATT_G
Cuts at: 7152 7152
Size: 9553
MluI A'CGCG_T
Cuts at: 9429 9429
Size: 9553
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2238 4476 4607 4960 8667 8851 1305
Size: 933 2238 131 353 3707 184 2007
Fragments arranged by size:
3707 2238 2007 933 353 184 131
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2576 3518 10
Size: 54 1425 343 744 942 6045
Fragments arranged by size:
6045 1425 942 744 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4430 4640 5120 5858 6587 7511 7870
Size: 648 3264 210 480 738 729 924 359
Cuts at: 7870 8029 518
Size: 159 2042
Fragments arranged by size:
3264 2042 924 738 729 648 480 359
210 159
MspA1I CmG'CkG
Cuts at: 1606 2464 2533 3079 3250 3919 4744 5128 6373
Size: 858 69 546 171 669 825 384 1245
Cuts at: 6373 6969 7646 8794 9039 1606
Size: 596 677 1148 245 2120
Fragments arranged by size:
2120 1245 1148 858 825 677 669 596
546 384 245 171 69
NaeI GCC'GGC
Cuts at: 3012 3410 5698 6172 6749 3012
Size: 398 2288 474 577 5816
Fragments arranged by size:
5816 2288 577 474 398
NcoI C'CATG_G
Cuts at: 513 1065 2229 2340 2868 3027 513
Size: 552 1164 111 528 159 7039
Fragments arranged by size:
7039 1164 552 528 159 111
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9553
NgoMIV G'CCGG_C
Cuts at: 3010 3408 5696 6170 6747 3010
Size: 398 2288 474 577 5816
Fragments arranged by size:
5816 2288 577 474 398
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9553
NotI GC'GGCC_GC
Cuts at: 6248 7000 6248
Size: 752 8801
NruI TCG'CGA
Cuts at: 6382 6382
Size: 9553
NsiI A_TGCA'T
Cuts at: 2244 2244
Size: 9553
NspI r_CATG'y
Cuts at: 1132 1229 2212 2257 2393 2785 3277 3452 3586
Size: 97 983 45 136 392 492 175 134
Cuts at: 3586 5005 5038 5158 5416 5704 6911 8456 1132
Size: 1419 33 120 258 288 1207 1545 2229
Fragments arranged by size:
2229 1545 1419 1207 983 492 392 288
258 175 136 134 120 97 45 33
PasI CC'CwG_GG
Cuts at: 1348 2026 2144 2179 2374 1348
Size: 678 118 35 195 8527
Fragments arranged by size:
8527 678 195 118 35
PciI A'CATG_T
Cuts at: 5001 5154 8452 5001
Size: 153 3298 6102
Fragments arranged by size:
6102 3298 153
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2414 2488 2853 3262 5221 1108
Size: 1306 74 365 409 1959 5440
Fragments arranged by size:
5440 1959 1306 409 365 74
PfoI T'CCnGG_A
Cuts at: 2833 5754 2833
Size: 2921 6632
PmeI GTTT'AAAC
Cuts at: 6857 6857
Size: 9553
PmlI CAC'GTG
Cuts at: 3868 4181 3868
Size: 313 9240
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5987 6019 7622 7654 9135 9167 5987
Size: 32 1603 32 1481 32 6373
Fragments arranged by size:
6373 1603 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2048 2102 2690 2726 2795 2864 3174 5551 6768
Size: 54 588 36 69 69 310 2377 1217
Cuts at: 6768 2048
Size: 4833
Fragments arranged by size:
4833 2377 1217 588 310 69 69 54
36
PshAI GACnn'nnGTC
Cuts at: 1613 4650 1613
Size: 3037 6516
PsiI TTA'TAA
Cuts at: 7123 7123
Size: 9553
PspOMI G'GGCC_C
Cuts at: 2059 2089 2135 2324 2429 2506 2774 4415 4479
Size: 30 46 189 105 77 268 1641 64
Cuts at: 4479 2059
Size: 7133
Fragments arranged by size:
7133 1641 268 189 105 77 64 46
30
PstI C_TGCA'G
Cuts at: 830 2607 3108 3129 4191 4749 4814 5016 5656
Size: 1777 501 21 1062 558 65 202 640
Cuts at: 5656 5748 6905 830
Size: 92 1157 3478
Fragments arranged by size:
3478 1777 1157 1062 640 558 501 202
92 65 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9553
PvuII CAG'CTG
Cuts at: 2533 3250 3919 5128 6373 2533
Size: 717 669 1209 1245 5713
Fragments arranged by size:
5713 1245 1209 717 669
RsrII CG'GwC_CG
Cuts at: 6684 6684
Size: 9553
SacI G_AGCT'C
Cuts at: 2551 3216 4767 5934 6411 2551
Size: 665 1551 1167 477 5693
Fragments arranged by size:
5693 1551 1167 665 477
SacII CC_GC'GG
Cuts at: 3080 3080
Size: 9553
SalI G'TCGA_C
Cuts at: 1936 6895 1936
Size: 4959 4594
SanDI GG'GwC_CC
Cuts at: 2102 2726 3174 6768 2102
Size: 624 448 3594 4887
Fragments arranged by size:
4887 3594 624 448
SapI GCTCTTCn'nnn_
Cuts at: 4011 4011
Size: 9553
SbfI CC_TGCA'GG
Cuts at: 3108 5748 6905 3108
Size: 2640 1157 5756
Fragments arranged by size:
5756 2640 1157
ScaI AGT'ACT
Cuts at: 5450 6401 7801 9513 5450
Size: 951 1400 1712 5490
Fragments arranged by size:
5490 1712 1400 951
SfcI C'TryA_G
Cuts at: 826 1045 2603 3104 3125 4187 4745 4810 5012
Size: 219 1558 501 21 1062 558 65 202
Cuts at: 5012 5652 5744 6040 6901 8036 8717 8908 826
Size: 640 92 296 861 1135 681 191 1471
Fragments arranged by size:
1558 1471 1135 1062 861 681 640 558
501 296 219 202 191 92 65 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9553
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9553
SmaI CCC'GGG
Cuts at: 1872 2058 2100 2638 2724 2815 3004 3172 3945
Size: 186 42 538 86 91 189 168 773
Cuts at: 3945 5474 6880 1872
Size: 1529 1406 4545
Fragments arranged by size:
4545 1529 1406 773 538 189 186 168
91 86 42
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3215 3932 4943 6071 7657 8558
Size: 197 926 1272 717 1011 1128 1586 901
Cuts at: 8558 8820 820
Size: 262 1553
Fragments arranged by size:
1586 1553 1272 1128 1011 926 901 717
262 197
SnaBI TAC'GTA
Cuts at: 493 7344 493
Size: 6851 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9553
SphI G_CATG'C
Cuts at: 1132 2393 3277 3452 3586 5416 6911 1132
Size: 1261 884 175 134 1830 1495 3774
Fragments arranged by size:
3774 1830 1495 1261 884 175 134
SspI AAT'ATT
Cuts at: 5 52 7477 5
Size: 47 7425 2081
Fragments arranged by size:
7425 2081 47
StuI AGG'CCT
Cuts at: 1517 2486 2758 3317 3926 1517
Size: 969 272 559 609 7144
Fragments arranged by size:
7144 969 609 559 272
StyI C'CwwG_G
Cuts at: 513 1065 2229 2340 2356 2487 2868 3027 3453
Size: 552 1164 111 16 131 381 159 426
Cuts at: 3453 3507 3735 5043 5215 6992 513
Size: 54 228 1308 172 1777 3074
Fragments arranged by size:
3074 1777 1308 1164 552 426 381 228
172 159 131 111 54 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2192 2366 5583 6295 6565 6672 7580
Size: 1255 612 174 3217 712 270 107 908
Cuts at: 7580 7597 7750 7935 8380 325
Size: 17 153 185 445 1498
Fragments arranged by size:
3217 1498 1255 908 712 612 445 270
185 174 153 107 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3117 4308 4587
Size: 274 80 33 51 1443 1140 1191 279
Cuts at: 4587 4632 5448 5661 6327 6399 7799 9511 96
Size: 45 816 213 666 72 1400 1712 138
Fragments arranged by size:
1712 1443 1400 1191 1140 816 666 279
274 213 138 80 72 51 45 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6050 6082 9513 9545 6050
Size: 32 3431 32 6058
Fragments arranged by size:
6058 3431 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2995 1316
Size: 1679 7874
XbaI T'CTAG_A
Cuts at: 6889 6889
Size: 9553
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3872 4051 4550 3872
Size: 179 499 8875
Fragments arranged by size:
8875 499 179
XhoI C'TCGA_G
Cuts at: 1943 3932 1943
Size: 1989 7564
XmaI C'CCGG_G
Cuts at: 1870 2056 2098 2636 2722 2813 3002 3170 3943
Size: 186 42 538 86 91 189 168 773
Cuts at: 3943 5472 6878 1870
Size: 1529 1406 4545
Fragments arranged by size:
4545 1529 1406 773 538 189 186 168
91 86 42
XmnI GAAnn'nnTTC
Cuts at: 3165 4887 5426 6002 7682 3165
Size: 1722 539 576 1680 5036
Fragments arranged by size:
5036 1722 1680 576 539
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4096 6347 6496 7361
Size: 53 83 186 936 2562 2251 149 865
Cuts at: 7361 276
Size: 2468
Fragments arranged by size:
2562 2468 2251 936 865 186 149 83
53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AvrII BaeI BbeI BstZ17I EcoRV FalI FseI KasI
NarI PacI PspXI PsrI SexAI SfiI SfoI SrfI
SwaI