(Circular) (Six-base) MAPSORT of: pFN21AE6732.seq Check: 8357 from: 1 to: 8107 pFN21AE6732 8107 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3760 3922 4748 3760 Size: 162 826 7119 Fragments arranged by size: 7119 826 162 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5917 278 Size: 53 83 186 936 4381 2468 Fragments arranged by size: 4381 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4312 5450 1937 Size: 2375 1138 4594 Fragments arranged by size: 4594 2375 1138 Acc65I G'GTAC_C Cuts at: 5428 5428 Size: 8107 AclI AA'CG_TT Cuts at: 1563 6234 6607 1563 Size: 4671 373 3063 Fragments arranged by size: 4671 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2093 2315 3477 3935 4439 4566 6170 7554 Size: 461 222 1162 458 504 127 1604 1384 Cuts at: 7554 7822 1632 Size: 268 1917 Fragments arranged by size: 1917 1604 1384 1162 504 461 458 268 222 127 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7910 AflIII A'CryG_T Cuts at: 1203 3115 7006 7983 1203 Size: 1912 3891 977 1327 Fragments arranged by size: 3891 1912 1327 977 AgeI A'CCGG_T Cuts at: 6938 6938 Size: 8107 AhdI GACnn_n'nnGTC Cuts at: 6836 6836 Size: 8107 AleI CACnn'nnGTG Cuts at: 1166 2217 1166 Size: 1051 7056 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2281 3080 1184 Size: 1097 799 6211 Fragments arranged by size: 6211 1097 799 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2451 2483 2787 2819 2451 Size: 32 304 32 7739 Fragments arranged by size: 7739 304 32 32 AlwNI CAG_nnn'CTG Cuts at: 2171 4262 5850 7422 2171 Size: 2091 1588 1572 2856 Fragments arranged by size: 2856 2091 1588 1572 ApaLI G'TGCA_C Cuts at: 6164 7320 6164 Size: 1156 6951 ApoI r'AATT_y Cuts at: 1493 2082 2158 2514 3407 3617 4661 4999 5224 Size: 589 76 356 893 210 1044 338 225 Cuts at: 5224 5260 5309 5320 5416 5642 5794 7903 1493 Size: 36 49 11 96 226 152 2109 1697 Fragments arranged by size: 2109 1697 1044 893 589 356 338 226 225 210 152 96 76 49 36 11 AseI AT'TA_AT Cuts at: 160 1032 5156 6661 160 Size: 872 4124 1505 1606 Fragments arranged by size: 4124 1606 1505 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8107 AvaI C'yCGr_G Cuts at: 1870 1943 5432 1870 Size: 73 3489 4545 Fragments arranged by size: 4545 3489 73 AvrII C'CTAG_G Cuts at: 2065 4882 2065 Size: 2817 5290 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2417 2450 4574 4607 2417 Size: 33 2124 33 5917 Fragments arranged by size: 5917 2124 33 33 BamHI G'GATC_C Cuts at: 4422 5355 5437 5804 4422 Size: 933 82 367 6725 Fragments arranged by size: 6725 933 367 82 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3809 5362 5377 5428 6883 Size: 325 217 627 2022 1553 15 51 1455 Cuts at: 6883 618 Size: 1842 Fragments arranged by size: 2022 1842 1553 1455 627 325 217 51 15 BanII G_rGCy'C Cuts at: 1391 2933 5393 5426 1391 Size: 1542 2460 33 4072 Fragments arranged by size: 4072 2460 1542 33 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2474 3099 3201 3972 4618 4783 928 Size: 971 575 625 102 771 646 165 4252 Fragments arranged by size: 4252 971 771 646 625 575 165 102 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6298 6332 1903 Size: 34 4361 34 3678 Fragments arranged by size: 4361 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3321 3333 5999 7209 3321 Size: 12 2666 1210 4219 Fragments arranged by size: 4219 2666 1210 12 BclI T'GATC_A Cuts at: 1548 3028 1548 Size: 1480 6627 BfrBI ATG'CAT Cuts at: 5248 5248 Size: 8107 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6718 136 Size: 107 122 71 631 605 5046 1525 Fragments arranged by size: 5046 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3080 4092 8102 3080 Size: 1012 4010 3085 Fragments arranged by size: 4010 3085 1012 BlpI GC'TnA_GC Cuts at: 5524 5524 Size: 8107 Bme1580I G_kGCm'C Cuts at: 1790 6168 7324 1790 Size: 4378 1156 2573 Fragments arranged by size: 4378 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8107 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3321 3703 3818 Size: 416 53 83 399 24 1904 382 115 Cuts at: 3818 5838 6796 442 Size: 2020 958 1753 Fragments arranged by size: 2020 1904 1753 958 416 399 382 115 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8107 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4051 4051 Size: 8107 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2736 2814 3345 4046 4284 6767 1239 Size: 1497 78 531 701 238 2483 2579 Fragments arranged by size: 2579 2483 1497 701 531 238 78 Bpu10I CC'TnA_GC Cuts at: 5385 5385 Size: 8107 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2612 2917 6232 7097 7395 2612 Size: 305 3315 865 298 3324 Fragments arranged by size: 3324 3315 865 305 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2194 3149 4698 6770 882 Size: 617 695 955 1549 2072 2219 Fragments arranged by size: 2219 2072 1549 955 695 617 BsaAI yAC'GTr Cuts at: 493 1204 5898 493 Size: 711 4694 2702 Fragments arranged by size: 4694 2702 711 BsaBI GATnn'nnATC Cuts at: 3321 8101 3321 Size: 4780 3327 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2697 5914 6296 6931 Size: 53 83 186 936 1164 3217 382 635 Cuts at: 6931 275 Size: 1451 Fragments arranged by size: 3217 1451 1164 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4106 6540 6938 7212 7359 7770 4106 Size: 2434 398 274 147 411 4443 Fragments arranged by size: 4443 2434 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2787 2817 1178 Size: 30 165 30 1384 30 6468 Fragments arranged by size: 6468 1384 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4011 1186 Size: 2825 5282 BseYI C'CCAG_C Cuts at: 2762 3893 4353 7310 7806 2762 Size: 1131 460 2957 496 3063 Fragments arranged by size: 3063 2957 1131 496 460 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4146 1163 Size: 573 2410 5124 Fragments arranged by size: 5124 2410 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5557 6318 7346 1540 Size: 275 186 3556 761 1028 2301 Fragments arranged by size: 3556 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2211 2628 3361 4635 4938 6168 6253 7324 2211 Size: 417 733 1274 303 1230 85 1071 2994 Fragments arranged by size: 2994 1274 1230 1071 733 417 303 85 BsmI GAATG_Cn' Cuts at: 5618 5711 5618 Size: 93 8014 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2211 2628 2933 3361 4635 4938 5393 Size: 399 421 417 305 428 1274 303 455 Cuts at: 5393 5426 6168 6253 7324 1391 Size: 33 742 85 1071 2174 Fragments arranged by size: 2174 1274 1071 742 455 428 421 417 399 305 303 85 33 BspEI T'CCGG_A Cuts at: 7770 7770 Size: 8107 BspHI T'CATG_A Cuts at: 3031 4797 5996 7726 3031 Size: 1766 1199 1730 3412 Fragments arranged by size: 3412 1766 1730 1199 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2122 2176 3760 3922 4748 5462 844 Size: 1278 54 1584 162 826 714 3489 Fragments arranged by size: 3489 1584 1278 826 714 162 54 BsrBI CCG'CTC Cuts at: 1394 1823 5994 1394 Size: 429 4171 3507 Fragments arranged by size: 4171 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3143 3996 5190 6602 6776 65 Size: 1183 1895 853 1194 1412 174 1396 Fragments arranged by size: 1895 1412 1396 1194 1183 853 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6751 6938 1700 Size: 110 4941 187 2869 Fragments arranged by size: 4941 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3232 96 Size: 3136 4971 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8107 BssSI C'ACGA_G Cuts at: 6167 7179 6167 Size: 1012 7095 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8107 BstBI TT'CG_AA Cuts at: 6911 6911 Size: 8107 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8107 BstXI CCAn_nnnn'nTGG Cuts at: 2219 3879 2219 Size: 1660 6447 BstYI r'GATC_y Cuts at: 1972 2123 3080 4092 4422 4989 5355 5437 5804 Size: 151 957 1012 330 567 366 82 367 Cuts at: 5804 6189 6206 7658 7744 7756 8102 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1012 957 567 385 367 366 346 330 151 86 82 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2118 2180 3168 3764 4376 4586 Size: 552 939 114 62 988 596 612 210 Cuts at: 4586 513 Size: 4034 Fragments arranged by size: 4034 988 939 612 596 552 210 114 62 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2508 487 Size: 683 56 1282 6086 Fragments arranged by size: 6086 1282 683 56 BtsI GCAGTG_nn' Cuts at: 2263 2426 3064 3211 5623 6417 6437 2263 Size: 163 638 147 2412 794 20 3933 Fragments arranged by size: 3933 2412 794 638 163 147 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8107 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8072 DraI TTT'AAA Cuts at: 3544 3612 5049 5411 3544 Size: 68 1437 362 6240 Fragments arranged by size: 6240 1437 362 68 DraIII CAC_nnn'GTG Cuts at: 3339 3853 3339 Size: 514 7593 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3401 7114 809 Size: 507 2085 3713 1802 Fragments arranged by size: 3713 2085 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 2050 3761 Size: 54 1425 50 275 18 186 34 1711 Cuts at: 3761 4529 5554 6443 8072 8 Size: 768 1025 889 1629 43 Fragments arranged by size: 1711 1629 1425 1025 889 768 275 186 54 50 43 34 18 EagI C'GGCC_G Cuts at: 1537 1812 5554 1537 Size: 275 3742 4090 Fragments arranged by size: 4090 3742 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2731 6037 1353 Size: 1378 3306 3423 Fragments arranged by size: 3423 3306 1378 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6694 7068 7214 387 Size: 1064 138 5105 374 146 1280 Fragments arranged by size: 5105 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2093 2315 2736 2814 3345 3477 3935 Size: 393 461 222 421 78 531 132 458 Cuts at: 3935 4046 4284 4439 4566 6170 6767 7554 7822 Size: 111 238 155 127 1604 597 787 268 Cuts at: 7822 1239 Size: 1524 Fragments arranged by size: 1604 1524 787 597 531 461 458 421 393 268 238 222 155 132 127 111 78 EcoO109I rG'GnC_Cy Cuts at: 2950 2950 Size: 8107 EcoRI G'AATT_C Cuts at: 2082 2514 5416 5794 2082 Size: 432 2902 378 4395 Fragments arranged by size: 4395 2902 432 378 FspI TGC'GCA Cuts at: 6613 6613 Size: 8107 HaeII r_GCGC'y Cuts at: 1678 7254 1678 Size: 5576 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2161 2193 3026 3058 3798 Size: 32 135 32 589 32 833 32 740 Cuts at: 3798 3830 4392 4424 4508 4540 4664 4696 6748 Size: 32 562 32 84 32 124 32 2052 Cuts at: 6748 6780 6822 6854 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2052 833 740 589 562 135 124 84 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2095 3384 3495 5451 5697 669 Size: 988 281 157 1289 111 1956 246 3079 Fragments arranged by size: 3079 1956 1289 988 281 246 157 111 HindIII A'AGCT_T Cuts at: 748 2227 748 Size: 1479 6628 HpaI GTT'AAC Cuts at: 5697 5697 Size: 8107 KpnI G_GTAC'C Cuts at: 5432 5432 Size: 8107 MfeI C'AATT_G Cuts at: 3857 4490 5706 3857 Size: 633 1216 6258 Fragments arranged by size: 6258 1216 633 MluI A'CGCG_T Cuts at: 7983 7983 Size: 8107 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2132 3001 5269 7221 7405 1305 Size: 827 869 2268 1952 184 2007 Fragments arranged by size: 2268 2007 1952 869 827 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2018 3763 4531 10 Size: 54 1425 343 186 1745 768 3586 Fragments arranged by size: 3586 1745 1425 768 343 186 54 MslI CAynn'nnrTG Cuts at: 518 1166 2217 3036 3530 3956 4306 4320 5121 Size: 648 1051 819 494 426 350 14 801 Cuts at: 5121 6065 6424 6583 518 Size: 944 359 159 2042 Fragments arranged by size: 2042 1051 944 819 801 648 494 426 359 350 159 14 MspA1I CmG'CkG Cuts at: 1606 2293 4353 4813 5523 6200 7348 7593 1606 Size: 687 2060 460 710 677 1148 245 2120 Fragments arranged by size: 2120 2060 1148 710 687 677 460 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2180 3168 3764 4376 513 Size: 552 939 176 988 596 612 4244 Fragments arranged by size: 4244 988 939 612 596 552 176 NdeI CA'TA_TG Cuts at: 387 387 Size: 8107 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8107 NotI GC'GGCC_GC Cuts at: 5554 5554 Size: 8107 NsiI A_TGCA'T Cuts at: 5250 5250 Size: 8107 NspI r_CATG'y Cuts at: 1132 1229 4172 5465 7010 1132 Size: 97 2943 1293 1545 2229 Fragments arranged by size: 2943 2229 1545 1293 97 PasI CC'CwG_GG Cuts at: 1348 2650 1348 Size: 1302 6805 PciI A'CATG_T Cuts at: 7006 7006 Size: 8107 PflMI CCAn_nnn'nTGG Cuts at: 1108 2535 1108 Size: 1427 6680 PfoI T'CCnGG_A Cuts at: 3878 4216 4804 5351 3878 Size: 338 588 547 6634 Fragments arranged by size: 6634 588 547 338 PmeI GTTT'AAAC Cuts at: 5411 5411 Size: 8107 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2787 2819 6176 6208 7689 7721 2787 Size: 32 3357 32 1481 32 3173 Fragments arranged by size: 3357 3173 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8107 PsiI TTA'TAA Cuts at: 3377 5677 3377 Size: 2300 5807 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3615 3647 3615 Size: 32 8075 PstI C_TGCA'G Cuts at: 830 5459 830 Size: 4629 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8107 PvuII CAG'CTG Cuts at: 2293 4353 2293 Size: 2060 6047 SalI G'TCGA_C Cuts at: 1936 5449 1936 Size: 3513 4594 SbfI CC_TGCA'GG Cuts at: 5459 5459 Size: 8107 ScaI AGT'ACT Cuts at: 6355 8067 6355 Size: 1712 6395 SfcI C'TryA_G Cuts at: 826 1045 4151 5455 6590 7271 7462 826 Size: 219 3106 1304 1135 681 191 1471 Fragments arranged by size: 3106 1471 1304 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8107 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8107 SmaI CCC'GGG Cuts at: 1872 5434 1872 Size: 3562 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2627 2932 6211 7112 7374 820 Size: 197 926 684 305 3279 901 262 1553 Fragments arranged by size: 3279 1553 926 901 684 305 262 197 SnaBI TAC'GTA Cuts at: 493 5898 493 Size: 5405 2702 SpeI A'CTAG_T Cuts at: 152 4303 152 Size: 4151 3956 SphI G_CATG'C Cuts at: 1132 5465 1132 Size: 4333 3774 SspI AAT'ATT Cuts at: 5 52 2359 2564 3132 3510 5071 6031 5 Size: 47 2307 205 568 378 1561 960 2081 Fragments arranged by size: 2307 2081 1561 960 568 378 205 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8107 StyI C'CwwG_G Cuts at: 513 1065 2004 2065 2180 3168 3764 4376 4882 Size: 552 939 61 115 988 596 612 506 Cuts at: 4882 5546 513 Size: 664 3074 Fragments arranged by size: 3074 988 939 664 612 596 552 506 115 61 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4516 4618 6134 6151 6304 6489 6934 Size: 1255 2936 102 1516 17 153 185 445 Cuts at: 6934 325 Size: 1498 Fragments arranged by size: 2936 1516 1498 1255 445 185 153 102 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2322 3232 4029 Size: 274 80 33 51 1443 345 910 797 Cuts at: 4029 6353 8065 96 Size: 2324 1712 138 Fragments arranged by size: 2324 1712 1443 910 797 345 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4372 4404 4800 4832 8067 8099 4372 Size: 32 396 32 3235 32 4380 Fragments arranged by size: 4380 3235 396 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8107 XbaI T'CTAG_A Cuts at: 2126 2138 5443 2126 Size: 12 3305 4790 Fragments arranged by size: 4790 3305 12 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2711 2972 3166 4289 2011 Size: 700 261 194 1123 5829 Fragments arranged by size: 5829 1123 700 261 194 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8107 XmaI C'CCGG_G Cuts at: 1870 5432 1870 Size: 3562 4545 XmnI GAAnn'nnTTC Cuts at: 4274 6236 4274 Size: 1962 6145 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5915 276 Size: 53 83 186 936 4381 2468 Fragments arranged by size: 4381 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BbeI BbvCI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI