################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AseI AsnI Asp700I Asp718I AspEI AvrII BbrPI BcgI BciVI BfaI BfrI BglII BlnI BpmI BsiEI BsmI Bsp1407I BspDI BspHI BspMI BsrDI BsrGI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoT22I HpaI KpnI MaeI MfeI MluI MunI NdeI NheI NotI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SalI SbfI ScaI SexAI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tsp509I XbaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccIII 999 TCCGGA T^CCGGA AscI 191 GGCGCGCC GG^CGCGCC AspI 1536 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1995 GGATCC G^GATCC BclI 272 TGATCA T^GATCA BglI 1190 GCCTCTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 532 GAGACC GGTCTC(1/5) BsaWI 999 TCCGGA [AT]^CCGG[AT] BseAI 999 TCCGGA T^CCGGA BsgI 466 CTGCAC GTGCAG(16/14) BsiWI 434 CGTACG C^GTACG BspEI 999 TCCGGA T^CCGGA BspLU11I 1523 ACATGT A^CATGT BssHII 192 GCGCGC G^CGCGC BssSI 2020 CACGAG CACGAG(-5/-1) BstXI 866 CCAGGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EcoNI 495 CCTGTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 2262 GATATC GAT^ATC FbaI 272 TGATCA T^GATCA FseI 342 GGCCGGCC GGCCGG^CC HincII 1615 GTCAAC GT[CT]^[GA]AC HindII 1615 GTCAAC GT[CT]^[GA]AC HindIII 2223 AAGCTT A^AGCTT MroI 999 TCCGGA T^CCGGA MseI 1130 TTAA T^TAA NruI 576 TCGCGA TCG^CGA PaeR7I 1180 CTCGAG C^TCGAG PflFI 1536 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1532 CCAAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 842 AGGACCC [GA]G^G[AT]CC[CT] PvuII 2006 CAGCTG CAG^CTG SapI 834 GCTCTTC GCTCTTC(1/4) SfcI 2100 CTATAG C^T[GA][CT]AG SfiI 1189 GGCCTCTCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Tru9I 1130 TTAA T^TAA Tth111I 1536 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1532 CCAAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1180 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AflIII 482 ACGTGT A^C[GA][CT]GT 1523 ACATGT AlwNI 1657 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG 2003 CAGCAGCTG BbsI 2062 GAAGAC GAAGAC(2/6) 427 GTCTTC BbvCI 1200 CCTCAGC CCTCAGC(-5/-2) 583 GCTGAGG BlpI 3 GCTGAGC GC^T[ACGT]AGC 1405 GCTGAGC Bpu1102I 3 GCTGAGC GC^T[ACGT]AGC 1405 GCTGAGC BpuAI 2062 GAAGAC GAAGAC(2/6) 427 GTCTTC BsaAI 436 TACGTG [CT]AC^GT[GA] 1048 TACGTG BsaBI 267 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1959 GATTGAGATC BsrI 2173 ACTGG ACTGG(1/-1) 2201 ACTGG BstAPI 1656 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2002 GCAGCAGCTGC BstEII 966 GGTGACC G^GT[ACGT]ACC 1494 GGTCACC BstPI 966 GGTGACC G^GT[ACGT]ACC 1494 GGTCACC BstYI 1977 AGATCC [GA]^GATC[CT] 1995 GGATCC CelII 3 GCTGAGC GC^T[ACGT]AGC 1405 GCTGAGC DraIII 798 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG 1288 CACTTCGTG EarI 835 CTCTTC CTCTTC(1/4) 889 CTCTTC EcoO65I 966 GGTGACC G^GT[ACGT]ACC 1494 GGTCACC Ksp632I 835 CTCTTC CTCTTC(1/4) 889 CTCTTC MamI 267 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1959 GATTGAGATC MflI 1977 AGATCC [GA]^GATC[CT] 1995 GGATCC NspI 1523 ACATGT [GA]CATG^[CT] 1628 GCATGT SmlI 724 CTCAAG C^T[CT][GA]AG 1180 CTCGAG XhoII 1977 AGATCC [GA]^GATC[CT] 1995 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI ApaI AspHI AvaI AvaII AviII BalI BanI BanII BbeI BbvI BcnI BmyI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BsrBI BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KspI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV PleI RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfoI SmaI StuI StyI TaqI TseI Tsp45I TspRI TthHB8I XcmI XmaCI XmaI