(Circular) (Six-base) MAPSORT of: pFN21AE3951.seq Check: 2047 from: 1 to: 6976 pFN21AE3951 6976 bp With 182 enzymes: SgfI * July 6, 2012 17:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4786 278 Size: 53 83 186 936 3250 2468 Fragments arranged by size: 3250 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4319 1937 Size: 2382 4594 Acc65I G'GTAC_C Cuts at: 4297 4297 Size: 6976 AclI AA'CG_TT Cuts at: 1563 5103 5476 1563 Size: 3540 373 3063 Fragments arranged by size: 3540 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5039 6423 6691 1632 Size: 3407 1384 268 1917 Fragments arranged by size: 3407 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6779 AflIII A'CryG_T Cuts at: 1203 2487 3528 5875 6852 1203 Size: 1284 1041 2347 977 1327 Fragments arranged by size: 2347 1327 1284 1041 977 AgeI A'CCGG_T Cuts at: 5807 5807 Size: 6976 AhdI GACnn_n'nnGTC Cuts at: 5705 5705 Size: 6976 AleI CACnn'nnGTG Cuts at: 1166 3020 1166 Size: 1854 5122 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2148 1184 Size: 964 6012 AlwNI CAG_nnn'CTG Cuts at: 3667 4013 4719 6291 3667 Size: 346 706 1572 4352 Fragments arranged by size: 4352 1572 706 346 ApaI G_GGCC'C Cuts at: 2168 2187 2499 3855 2168 Size: 19 312 1356 5289 Fragments arranged by size: 5289 1356 312 19 ApaLI G'TGCA_C Cuts at: 5033 6189 5033 Size: 1156 5820 ApoI r'AATT_y Cuts at: 1493 4285 4511 4663 6772 1493 Size: 2792 226 152 2109 1697 Fragments arranged by size: 2792 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 2197 2197 Size: 6976 AseI AT'TA_AT Cuts at: 160 1032 5530 160 Size: 872 4498 1606 Fragments arranged by size: 4498 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6976 AvaI C'yCGr_G Cuts at: 1870 1943 2087 2186 2213 2363 2392 2425 2993 Size: 73 144 99 27 150 29 33 568 Cuts at: 2993 3185 3199 3866 4301 1870 Size: 192 14 667 435 4545 Fragments arranged by size: 4545 667 568 435 192 150 144 99 73 33 29 27 14 BamHI G'GATC_C Cuts at: 4000 4306 4673 4000 Size: 306 367 6303 Fragments arranged by size: 6303 367 306 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2040 2068 2173 2396 2421 Size: 325 217 627 253 28 105 223 25 Cuts at: 2421 2614 2630 2657 2762 2811 2998 3422 4297 Size: 193 16 27 105 49 187 424 875 Cuts at: 4297 5752 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 875 627 424 325 253 223 217 193 187 105 105 49 28 27 25 16 BanII G_rGCy'C Cuts at: 1391 2015 2168 2187 2364 2499 2517 2730 3042 Size: 624 153 19 177 135 18 213 312 Cuts at: 3042 3449 3716 3855 4079 4295 1391 Size: 407 267 139 224 216 4072 Fragments arranged by size: 4072 624 407 312 267 224 216 213 177 153 139 135 19 18 BbeI G_GCGC'C Cuts at: 2044 2072 2400 2618 2634 2661 2766 2815 3426 Size: 28 328 218 16 27 105 49 611 Cuts at: 3426 2044 Size: 5594 Fragments arranged by size: 5594 611 328 218 105 49 28 27 16 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2425 4074 928 Size: 971 526 1649 3830 Fragments arranged by size: 3830 1649 971 526 BbvCI CC'TCA_GC Cuts at: 2589 3206 2589 Size: 617 6359 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5167 5201 1903 Size: 34 50 34 3146 34 3678 Fragments arranged by size: 3678 3146 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4868 6078 4868 Size: 1210 5766 BclI T'GATC_A Cuts at: 1548 2277 1548 Size: 729 6247 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3201 5587 136 Size: 107 122 71 631 605 1529 2386 1525 Fragments arranged by size: 2386 1529 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6971 6971 Size: 6976 BlpI GC'TnA_GC Cuts at: 2009 3411 4393 2009 Size: 1402 982 4592 Fragments arranged by size: 4592 1402 982 Bme1580I G_kGCm'C Cuts at: 1790 2168 2187 2499 3001 3577 3612 3855 5037 Size: 378 19 312 502 576 35 243 1182 Cuts at: 5037 6193 1790 Size: 1156 2573 Fragments arranged by size: 2573 1182 1156 576 502 378 312 243 35 19 BmgBI CAC'GTC Cuts at: 1318 2488 2545 3229 3543 1318 Size: 1170 57 684 314 4751 Fragments arranged by size: 4751 1170 684 314 57 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4707 5665 442 Size: 416 53 83 399 24 3290 958 1753 Fragments arranged by size: 3290 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6976 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5636 1239 Size: 4397 2579 Bpu10I CC'TnA_GC Cuts at: 2589 3206 3875 3951 2589 Size: 617 669 76 5614 Fragments arranged by size: 5614 669 617 76 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2714 5101 5966 6264 2714 Size: 2387 865 298 3426 Fragments arranged by size: 3426 2387 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2531 5639 882 Size: 617 1032 3108 2219 Fragments arranged by size: 3108 2219 1032 617 BsaAI yAC'GTr Cuts at: 493 1204 2443 3055 4767 493 Size: 711 1239 612 1712 2702 Fragments arranged by size: 2702 1712 1239 711 612 BsaBI GATnn'nnATC Cuts at: 2276 3968 6970 2276 Size: 1692 3002 2282 Fragments arranged by size: 3002 2282 1692 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2041 2069 2397 2615 Size: 53 83 186 936 508 28 328 218 Cuts at: 2615 2631 2658 2763 2812 3423 4783 5165 5800 Size: 16 27 105 49 611 1360 382 635 Cuts at: 5800 275 Size: 1451 Fragments arranged by size: 1451 1360 936 635 611 508 382 328 218 186 105 83 53 49 28 27 16 BsaWI w'CCGG_w Cuts at: 3004 5409 5807 6081 6228 6639 3004 Size: 2405 398 274 147 411 3341 Fragments arranged by size: 3341 2405 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3050 3080 3309 3339 4168 Size: 30 165 30 1647 30 229 30 829 Cuts at: 4168 4198 1178 Size: 30 3956 Fragments arranged by size: 3956 1647 829 229 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2522 2525 2606 3906 3971 4121 1186 Size: 1336 3 81 1300 65 150 4041 Fragments arranged by size: 4041 1336 1300 150 81 65 3 BseYI C'CCAG_C Cuts at: 2479 3232 3313 3844 3895 4036 6179 6675 2479 Size: 753 81 531 51 141 2143 496 2780 Fragments arranged by size: 2780 2143 753 531 496 141 81 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2456 1163 Size: 573 720 5683 Fragments arranged by size: 5683 720 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4426 5187 6215 1540 Size: 275 186 2425 761 1028 2301 Fragments arranged by size: 2425 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2015 2448 2517 2730 3384 4186 5037 5122 6193 Size: 433 69 213 654 802 851 85 1071 Cuts at: 6193 2015 Size: 2798 Fragments arranged by size: 2798 1071 851 802 654 433 213 85 69 BsiWI C'GTAC_G Cuts at: 2439 2439 Size: 6976 BsmI GAATG_Cn' Cuts at: 4487 4580 4487 Size: 93 6883 BsmBI CGTCTCn'nnnn_ Cuts at: 2029 2297 3026 2029 Size: 268 729 5979 Fragments arranged by size: 5979 729 268 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2168 2187 2364 2448 2499 2517 Size: 399 225 153 19 177 84 51 18 Cuts at: 2517 2730 3001 3042 3384 3449 3577 3612 3716 Size: 213 271 41 342 65 128 35 104 Cuts at: 3716 3855 4079 4186 4295 5037 5122 6193 1391 Size: 139 224 107 109 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 342 271 225 224 213 177 153 139 128 109 107 104 85 84 65 51 41 35 19 18 BspEI T'CCGG_A Cuts at: 3004 6639 3004 Size: 3635 3341 BspHI T'CATG_A Cuts at: 4865 6595 4865 Size: 1730 5246 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4331 844 Size: 3487 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2313 2717 3887 4863 1394 Size: 429 490 404 1170 976 3507 Fragments arranged by size: 3507 1170 976 490 429 404 BsrDI GCAATG_nn' Cuts at: 65 1248 5471 5645 65 Size: 1183 4223 174 1396 Fragments arranged by size: 4223 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2065 2193 2221 2348 2418 2462 2571 Size: 110 255 128 28 127 70 44 109 Cuts at: 2571 5620 5807 1700 Size: 3049 187 2869 Fragments arranged by size: 3049 2869 255 187 128 127 110 109 70 44 28 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6976 BssHII G'CGCG_C Cuts at: 1925 2197 1925 Size: 272 6704 BssSI C'ACGA_G Cuts at: 4025 5036 6048 4025 Size: 1011 1012 4953 Fragments arranged by size: 4953 1012 1011 BstAPI GCAn_nnn'nTGC Cuts at: 794 3667 4013 794 Size: 2873 346 3757 Fragments arranged by size: 3757 2873 346 BstBI TT'CG_AA Cuts at: 5780 5780 Size: 6976 BstEII G'GTnAC_C Cuts at: 1184 2971 3499 1184 Size: 1787 528 4661 Fragments arranged by size: 4661 1787 528 BstXI CCAn_nnnn'nTGG Cuts at: 2878 2878 Size: 6976 BstYI r'GATC_y Cuts at: 1972 3982 4000 4306 4673 5058 5075 6527 6613 Size: 2010 18 306 367 385 17 1452 86 Cuts at: 6613 6625 6971 1972 Size: 12 346 1977 Fragments arranged by size: 2010 1977 1452 385 367 346 306 86 18 17 12 BtgI C'CryG_G Cuts at: 513 1065 2035 2179 2232 2625 3651 3769 3816 Size: 552 970 144 53 393 1026 118 47 Cuts at: 3816 4096 4257 513 Size: 280 161 3232 Fragments arranged by size: 3232 1026 970 552 393 280 161 144 118 53 47 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2564 3580 487 Size: 683 56 1338 1016 3883 Fragments arranged by size: 3883 1338 1016 683 56 BtsI GCAGTG_nn' Cuts at: 2473 4190 4492 5286 5306 2473 Size: 1717 302 794 20 4143 Fragments arranged by size: 4143 1717 794 302 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6976 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6941 DraI TTT'AAA Cuts at: 4280 4280 Size: 6976 DraIII CAC_nnn'GTG Cuts at: 2808 3298 2808 Size: 490 6486 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5983 809 Size: 507 4667 1802 Fragments arranged by size: 4667 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2229 2346 3165 Size: 54 1425 50 275 18 399 117 819 Cuts at: 3165 3648 3654 3831 4099 4423 5312 6941 8 Size: 483 6 177 268 324 889 1629 43 Fragments arranged by size: 1629 1425 889 819 483 399 324 275 268 177 117 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 4423 1537 Size: 275 2611 4090 Fragments arranged by size: 4090 2611 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2846 2900 4906 1353 Size: 1493 54 2006 3423 Fragments arranged by size: 3423 2006 1493 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5563 5937 6083 387 Size: 1064 138 3974 374 146 1280 Fragments arranged by size: 3974 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2013 2515 2728 2013 Size: 502 213 6261 Fragments arranged by size: 6261 502 213 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 5039 5636 6423 6691 1239 Size: 393 3407 597 787 268 1524 Fragments arranged by size: 3407 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2504 2504 Size: 6976 EcoO109I rG'GnC_Cy Cuts at: 2165 2496 2848 3837 3851 3852 4220 2165 Size: 331 352 989 14 1 368 4921 Fragments arranged by size: 4921 989 368 352 331 14 1 EcoRI G'AATT_C Cuts at: 4285 4663 4285 Size: 378 6598 EcoRV GAT'ATC Cuts at: 4269 4269 Size: 6976 FseI GG_CCGG'CC Cuts at: 2352 2352 Size: 6976 FspI TGC'GCA Cuts at: 2136 2324 3101 3272 5482 2136 Size: 188 777 171 2210 3630 Fragments arranged by size: 3630 2210 777 188 171 FspAI rTGC'GCAy Cuts at: 3101 3101 Size: 6976 HaeII r_GCGC'y Cuts at: 1678 2044 2072 2400 2600 2618 2634 2661 2737 Size: 366 28 328 200 18 16 27 76 Cuts at: 2737 2766 2815 2840 3426 3569 6123 1678 Size: 29 49 25 586 143 2554 2531 Fragments arranged by size: 2554 2531 586 366 328 200 143 76 49 29 28 27 25 18 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2840 2872 3309 3341 5617 Size: 32 135 32 1268 32 437 32 2276 Cuts at: 5617 5649 5691 5723 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2276 1268 437 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3622 4320 4566 669 Size: 988 281 1684 698 246 3079 Fragments arranged by size: 3079 1684 988 698 281 246 HindIII A'AGCT_T Cuts at: 748 4228 748 Size: 3480 3496 HpaI GTT'AAC Cuts at: 4566 4566 Size: 6976 KasI G'GCGC_C Cuts at: 2040 2068 2396 2614 2630 2657 2762 2811 3422 Size: 28 328 218 16 27 105 49 611 Cuts at: 3422 2040 Size: 5594 Fragments arranged by size: 5594 611 328 218 105 49 28 27 16 KpnI G_GTAC'C Cuts at: 4301 4301 Size: 6976 MfeI C'AATT_G Cuts at: 4575 4575 Size: 6976 MluI A'CGCG_T Cuts at: 6852 6852 Size: 6976 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3474 6090 6274 1305 Size: 2169 2616 184 2007 Fragments arranged by size: 2616 2169 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3167 3650 3656 10 Size: 54 1425 343 1335 483 6 3330 Fragments arranged by size: 3330 1425 1335 483 343 54 6 MslI CAynn'nnrTG Cuts at: 518 1166 3020 3599 3605 3821 4934 5293 5452 Size: 648 1854 579 6 216 1113 359 159 Cuts at: 5452 518 Size: 2042 Fragments arranged by size: 2042 1854 1113 648 579 359 216 159 6 MspA1I CmG'CkG Cuts at: 1606 2037 2110 2181 2265 2627 2817 3777 3848 Size: 431 73 71 84 362 190 960 71 Cuts at: 3848 4013 4259 4392 5069 6217 6462 1606 Size: 165 246 133 677 1148 245 2120 Fragments arranged by size: 2120 1148 960 677 431 362 246 245 190 165 133 84 73 71 71 NaeI GCC'GGC Cuts at: 2067 2223 2350 2067 Size: 156 127 6693 Fragments arranged by size: 6693 156 127 NarI GG'CG_CC Cuts at: 2041 2069 2397 2615 2631 2658 2763 2812 3423 Size: 28 328 218 16 27 105 49 611 Cuts at: 3423 2041 Size: 5594 Fragments arranged by size: 5594 611 328 218 105 49 28 27 16 NcoI C'CATG_G Cuts at: 513 1065 2232 3651 3816 513 Size: 552 1167 1419 165 3673 Fragments arranged by size: 3673 1419 1167 552 165 NdeI CA'TA_TG Cuts at: 387 387 Size: 6976 NgoMIV G'CCGG_C Cuts at: 2065 2221 2348 2065 Size: 156 127 6693 Fragments arranged by size: 6693 156 127 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6976 NotI GC'GGCC_GC Cuts at: 4423 4423 Size: 6976 NruI TCG'CGA Cuts at: 2583 2583 Size: 6976 NspI r_CATG'y Cuts at: 1132 1229 3532 3637 4334 5879 1132 Size: 97 2303 105 697 1545 2229 Fragments arranged by size: 2303 2229 1545 697 105 97 PasI CC'CwG_GG Cuts at: 1348 3373 3514 1348 Size: 2025 141 4810 Fragments arranged by size: 4810 2025 141 PciI A'CATG_T Cuts at: 3528 5875 3528 Size: 2347 4629 PflMI CCAn_nnn'nTGG Cuts at: 1108 3543 1108 Size: 2435 4541 PfoI T'CCnGG_A Cuts at: 2745 2745 Size: 6976 PmeI GTTT'AAAC Cuts at: 4280 4280 Size: 6976 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5045 5077 6558 6590 5045 Size: 32 1481 32 5431 Fragments arranged by size: 5431 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2848 2848 Size: 6976 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6976 PsiI TTA'TAA Cuts at: 4546 4546 Size: 6976 PspOMI G'GGCC_C Cuts at: 2164 2183 2495 3851 2164 Size: 19 312 1356 5289 Fragments arranged by size: 5289 1356 312 19 PstI C_TGCA'G Cuts at: 830 4328 830 Size: 3498 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6976 PvuII CAG'CTG Cuts at: 4013 4013 Size: 6976 SacI G_AGCT'C Cuts at: 2015 2517 2730 2015 Size: 502 213 6261 Fragments arranged by size: 6261 502 213 SacII CC_GC'GG Cuts at: 2038 2182 2628 4260 2038 Size: 144 446 1632 4754 Fragments arranged by size: 4754 1632 446 144 SalI G'TCGA_C Cuts at: 1936 4318 1936 Size: 2382 4594 SapI GCTCTTCn'nnn_ Cuts at: 2846 2846 Size: 6976 SbfI CC_TGCA'GG Cuts at: 4328 4328 Size: 6976 ScaI AGT'ACT Cuts at: 5224 6936 5224 Size: 1712 5264 SfcI C'TryA_G Cuts at: 826 1045 4105 4324 5459 6140 6331 826 Size: 219 3060 219 1135 681 191 1471 Fragments arranged by size: 3060 1471 1135 681 219 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3201 3201 Size: 6976 SfoI GGC'GCC Cuts at: 2042 2070 2398 2616 2632 2659 2764 2813 3424 Size: 28 328 218 16 27 105 49 611 Cuts at: 3424 2042 Size: 5594 Fragments arranged by size: 5594 611 328 218 105 49 28 27 16 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6976 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6976 SmaI CCC'GGG Cuts at: 1872 2188 2215 2394 2427 2995 3868 4303 1872 Size: 316 27 179 33 568 873 435 4545 Fragments arranged by size: 4545 873 568 435 316 179 33 27 SmlI C'TyrA_G Cuts at: 820 1017 1943 2729 3185 5080 5981 6243 820 Size: 197 926 786 456 1895 901 262 1553 Fragments arranged by size: 1895 1553 926 901 786 456 262 197 SnaBI TAC'GTA Cuts at: 493 4767 493 Size: 4274 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6976 SphI G_CATG'C Cuts at: 1132 4334 1132 Size: 3202 3774 SspI AAT'ATT Cuts at: 5 52 4900 5 Size: 47 4848 2081 Fragments arranged by size: 4848 2081 47 StuI AGG'CCT Cuts at: 1517 2552 2673 2981 1517 Size: 1035 121 308 5512 Fragments arranged by size: 5512 1035 308 121 StyI C'CwwG_G Cuts at: 513 1065 2232 3213 3651 3816 4415 513 Size: 552 1167 981 438 165 599 3074 Fragments arranged by size: 3074 1167 981 599 552 438 165 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2555 3814 4131 5003 5020 5173 5358 Size: 1255 975 1259 317 872 17 153 185 Cuts at: 5358 5803 325 Size: 445 1498 Fragments arranged by size: 1498 1259 1255 975 872 445 317 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3636 5222 6934 Size: 274 80 33 51 1443 1659 1586 1712 Cuts at: 6934 96 Size: 138 Fragments arranged by size: 1712 1659 1586 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3050 3082 4168 4200 6936 6968 3050 Size: 32 1086 32 2736 32 3058 Fragments arranged by size: 3058 2736 1086 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3544 1316 Size: 2228 4748 XbaI T'CTAG_A Cuts at: 4312 4312 Size: 6976 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2926 3025 3229 3511 2926 Size: 99 204 282 6391 Fragments arranged by size: 6391 282 204 99 XhoI C'TCGA_G Cuts at: 1943 3185 1943 Size: 1242 5734 XmaI C'CCGG_G Cuts at: 1870 2186 2213 2392 2425 2993 3866 4301 1870 Size: 316 27 179 33 568 873 435 4545 Fragments arranged by size: 4545 873 568 435 316 179 33 27 XmnI GAAnn'nnTTC Cuts at: 5105 5105 Size: 6976 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4784 276 Size: 53 83 186 936 3250 2468 Fragments arranged by size: 3250 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BaeI BfrBI BplI BstZ17I Bsu36I FalI NsiI PacI PmlI PspXI PsrI RsrII SanDI SexAI SrfI SwaI