(Linear) (Six-base) MAPSORT of: KIEE3951.seq Check: 3791 from: 1 to: 2271 KIEE3951.orf With 182 enzymes: SgfI * July 3, 2012 18:21 .. AflIII A'CryG_T Cuts at: 0 482 1523 2271 Size: 482 1041 748 Fragments arranged by size: 1041 748 482 AleI CACnn'nnGTG Cuts at: 0 1015 2271 Size: 1015 1256 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 143 2271 Size: 143 2128 AlwNI CAG_nnn'CTG Cuts at: 0 1662 2008 2271 Size: 1662 346 263 Fragments arranged by size: 1662 346 263 ApaI G_GGCC'C Cuts at: 0 163 182 494 1850 2271 Size: 163 19 312 1356 421 Fragments arranged by size: 1356 421 312 163 19 AscI GG'CGCG_CC Cuts at: 0 192 2271 Size: 192 2079 AvaI C'yCGr_G Cuts at: 0 82 181 208 358 387 420 988 1180 Size: 82 99 27 150 29 33 568 192 Cuts at: 1180 1194 1861 2271 Size: 14 667 410 Fragments arranged by size: 667 568 410 192 150 99 82 33 29 27 14 BamHI G'GATC_C Cuts at: 0 1995 2271 Size: 1995 276 BanI G'GyrC_C Cuts at: 0 35 63 168 391 416 609 625 652 Size: 35 28 105 223 25 193 16 27 Cuts at: 652 757 806 993 1417 2271 Size: 105 49 187 424 854 Fragments arranged by size: 854 424 223 193 187 105 105 49 35 28 27 25 16 BanII G_rGCy'C Cuts at: 0 10 163 182 359 494 512 725 1037 Size: 10 153 19 177 135 18 213 312 Cuts at: 1037 1444 1711 1850 2074 2271 Size: 407 267 139 224 197 Fragments arranged by size: 407 312 267 224 213 197 177 153 139 135 19 18 10 BbeI G_GCGC'C Cuts at: 0 39 67 395 613 629 656 761 810 Size: 39 28 328 218 16 27 105 49 Cuts at: 810 1421 2271 Size: 611 850 Fragments arranged by size: 850 611 328 218 105 49 39 28 27 16 BbsI GAAGACnn'nnnn_ Cuts at: 0 420 2069 2271 Size: 420 1649 202 Fragments arranged by size: 1649 420 202 BbvCI CC'TCA_GC Cuts at: 0 584 1201 2271 Size: 584 617 1070 Fragments arranged by size: 1070 617 584 BclI T'GATC_A Cuts at: 0 272 2271 Size: 272 1999 BglI GCCn_nnn'nGGC Cuts at: 0 1196 2271 Size: 1196 1075 BlpI GC'TnA_GC Cuts at: 0 4 1406 2271 Size: 4 1402 865 Fragments arranged by size: 1402 865 4 Bme1580I G_kGCm'C Cuts at: 0 163 182 494 996 1572 1607 1850 2271 Size: 163 19 312 502 576 35 243 421 Fragments arranged by size: 576 502 421 312 243 163 35 19 BmgBI CAC'GTC Cuts at: 0 483 540 1224 1538 2271 Size: 483 57 684 314 733 Fragments arranged by size: 733 684 483 314 57 Bpu10I CC'TnA_GC Cuts at: 0 584 1201 1870 1946 2271 Size: 584 617 669 76 325 Fragments arranged by size: 669 617 584 325 76 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 709 2271 Size: 709 1562 BsaI GGTCTCn'nnnn_ Cuts at: 0 526 2271 Size: 526 1745 BsaAI yAC'GTr Cuts at: 0 438 1050 2271 Size: 438 612 1221 Fragments arranged by size: 1221 612 438 BsaBI GATnn'nnATC Cuts at: 0 271 1963 2271 Size: 271 1692 308 Fragments arranged by size: 1692 308 271 BsaHI Gr'CG_yC Cuts at: 0 36 64 392 610 626 653 758 807 Size: 36 28 328 218 16 27 105 49 Cuts at: 807 1418 2271 Size: 611 853 Fragments arranged by size: 853 611 328 218 105 49 36 28 27 16 BsaWI w'CCGG_w Cuts at: 0 999 2271 Size: 999 1272 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1045 1075 1304 1334 2163 2193 2271 Size: 1045 30 229 30 829 30 78 Fragments arranged by size: 1045 829 229 78 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 517 520 601 1901 1966 2116 2271 Size: 517 3 81 1300 65 150 155 Fragments arranged by size: 1300 517 155 150 81 65 3 BseYI C'CCAG_C Cuts at: 0 474 1227 1308 1839 1890 2031 2271 Size: 474 753 81 531 51 141 240 Fragments arranged by size: 753 531 474 240 141 81 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 451 2271 Size: 451 1820 BsiHKAI G_wGCw'C Cuts at: 0 10 443 512 725 1379 2181 2271 Size: 10 433 69 213 654 802 90 Fragments arranged by size: 802 654 433 213 90 69 10 BsiWI C'GTAC_G Cuts at: 0 434 2271 Size: 434 1837 BsmBI CGTCTCn'nnnn_ Cuts at: 0 24 292 1021 2271 Size: 24 268 729 1250 Fragments arranged by size: 1250 729 268 24 Bsp1286I G_dGCh'C Cuts at: 0 10 163 182 359 443 494 512 725 Size: 10 153 19 177 84 51 18 213 Cuts at: 725 996 1037 1379 1444 1572 1607 1711 1850 Size: 271 41 342 65 128 35 104 139 Cuts at: 1850 2074 2181 2271 Size: 224 107 90 Fragments arranged by size: 342 271 224 213 177 153 139 128 107 104 90 84 65 51 41 35 19 18 10 BspEI T'CCGG_A Cuts at: 0 999 2271 Size: 999 1272 BsrBI CCG'CTC Cuts at: 0 308 712 1882 2271 Size: 308 404 1170 389 Fragments arranged by size: 1170 404 389 308 BsrFI r'CCGG_y Cuts at: 0 60 188 216 343 413 457 566 2271 Size: 60 128 28 127 70 44 109 1705 Fragments arranged by size: 1705 128 127 109 70 60 44 28 BssHII G'CGCG_C Cuts at: 0 192 2271 Size: 192 2079 BssSI C'ACGA_G Cuts at: 0 2020 2271 Size: 2020 251 BstAPI GCAn_nnn'nTGC Cuts at: 0 1662 2008 2271 Size: 1662 346 263 Fragments arranged by size: 1662 346 263 BstEII G'GTnAC_C Cuts at: 0 966 1494 2271 Size: 966 528 777 Fragments arranged by size: 966 777 528 BstXI CCAn_nnnn'nTGG Cuts at: 0 873 2271 Size: 873 1398 BstYI r'GATC_y Cuts at: 0 1977 1995 2271 Size: 1977 18 276 Fragments arranged by size: 1977 276 18 BtgI C'CryG_G Cuts at: 0 30 174 227 620 1646 1764 1811 2091 Size: 30 144 53 393 1026 118 47 280 Cuts at: 2091 2252 2271 Size: 161 19 Fragments arranged by size: 1026 393 280 161 144 118 53 47 30 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 559 1575 2271 Size: 559 1016 696 Fragments arranged by size: 1016 696 559 BtsI GCAGTG_nn' Cuts at: 0 468 2185 2271 Size: 468 1717 86 Fragments arranged by size: 1717 468 86 DraIII CAC_nnn'GTG Cuts at: 0 803 1293 2271 Size: 803 490 978 Fragments arranged by size: 978 803 490 EaeI y'GGCC_r Cuts at: 0 224 341 1160 1643 1649 1826 2094 2271 Size: 224 117 819 483 6 177 268 177 Fragments arranged by size: 819 483 268 224 177 177 117 6 EarI CTCTTCn'nnn_ Cuts at: 0 841 895 2271 Size: 841 54 1376 Fragments arranged by size: 1376 841 54 EcoICRI GAG'CTC Cuts at: 0 8 510 723 2271 Size: 8 502 213 1548 Fragments arranged by size: 1548 502 213 8 EcoNI CCTnn'n_nnAGG Cuts at: 0 499 2271 Size: 499 1772 EcoO109I rG'GnC_Cy Cuts at: 0 160 491 843 1832 1846 1847 2215 2271 Size: 160 331 352 989 14 1 368 56 Fragments arranged by size: 989 368 352 331 160 56 14 1 EcoRV GAT'ATC Cuts at: 0 2264 2271 Size: 2264 7 FseI GG_CCGG'CC Cuts at: 0 347 2271 Size: 347 1924 FspI TGC'GCA Cuts at: 0 131 319 1096 1267 2271 Size: 131 188 777 171 1004 Fragments arranged by size: 1004 777 188 171 131 FspAI rTGC'GCAy Cuts at: 0 1096 2271 Size: 1096 1175 HaeII r_GCGC'y Cuts at: 0 39 67 395 595 613 629 656 732 Size: 39 28 328 200 18 16 27 76 Cuts at: 732 761 810 835 1421 1564 2271 Size: 29 49 25 586 143 707 Fragments arranged by size: 707 586 328 200 143 76 49 39 29 28 27 25 18 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 835 867 1304 1336 2271 Size: 835 32 437 32 935 Fragments arranged by size: 935 835 437 32 32 HincII GTy'rAC Cuts at: 0 1617 2271 Size: 1617 654 HindIII A'AGCT_T Cuts at: 0 2223 2271 Size: 2223 48 KasI G'GCGC_C Cuts at: 0 35 63 391 609 625 652 757 806 Size: 35 28 328 218 16 27 105 49 Cuts at: 806 1417 2271 Size: 611 854 Fragments arranged by size: 854 611 328 218 105 49 35 28 27 16 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1469 2271 Size: 1469 802 MscI TGG'CCA Cuts at: 0 1162 1645 1651 2271 Size: 1162 483 6 620 Fragments arranged by size: 1162 620 483 6 MslI CAynn'nnrTG Cuts at: 0 1015 1594 1600 1816 2271 Size: 1015 579 6 216 455 Fragments arranged by size: 1015 579 455 216 6 MspA1I CmG'CkG Cuts at: 0 32 105 176 260 622 812 1772 1843 Size: 32 73 71 84 362 190 960 71 Cuts at: 1843 2008 2254 2271 Size: 165 246 17 Fragments arranged by size: 960 362 246 190 165 84 73 71 71 32 17 NaeI GCC'GGC Cuts at: 0 62 218 345 2271 Size: 62 156 127 1926 Fragments arranged by size: 1926 156 127 62 NarI GG'CG_CC Cuts at: 0 36 64 392 610 626 653 758 807 Size: 36 28 328 218 16 27 105 49 Cuts at: 807 1418 2271 Size: 611 853 Fragments arranged by size: 853 611 328 218 105 49 36 28 27 16 NcoI C'CATG_G Cuts at: 0 227 1646 1811 2271 Size: 227 1419 165 460 Fragments arranged by size: 1419 460 227 165 NgoMIV G'CCGG_C Cuts at: 0 60 216 343 2271 Size: 60 156 127 1928 Fragments arranged by size: 1928 156 127 60 NruI TCG'CGA Cuts at: 0 578 2271 Size: 578 1693 NspI r_CATG'y Cuts at: 0 1527 1632 2271 Size: 1527 105 639 Fragments arranged by size: 1527 639 105 PasI CC'CwG_GG Cuts at: 0 1368 1509 2271 Size: 1368 141 762 Fragments arranged by size: 1368 762 141 PciI A'CATG_T Cuts at: 0 1523 2271 Size: 1523 748 PflMI CCAn_nnn'nTGG Cuts at: 0 1538 2271 Size: 1538 733 PfoI T'CCnGG_A Cuts at: 0 740 2271 Size: 740 1531 PpuMI rG'GwC_Cy Cuts at: 0 843 2271 Size: 843 1428 PspOMI G'GGCC_C Cuts at: 0 159 178 490 1846 2271 Size: 159 19 312 1356 425 Fragments arranged by size: 1356 425 312 159 19 PvuII CAG'CTG Cuts at: 0 2008 2271 Size: 2008 263 SacI G_AGCT'C Cuts at: 0 10 512 725 2271 Size: 10 502 213 1546 Fragments arranged by size: 1546 502 213 10 SacII CC_GC'GG Cuts at: 0 33 177 623 2255 2271 Size: 33 144 446 1632 16 Fragments arranged by size: 1632 446 144 33 16 SapI GCTCTTCn'nnn_ Cuts at: 0 841 2271 Size: 841 1430 SfcI C'TryA_G Cuts at: 0 2100 2271 Size: 2100 171 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1196 2271 Size: 1196 1075 SfoI GGC'GCC Cuts at: 0 37 65 393 611 627 654 759 808 Size: 37 28 328 218 16 27 105 49 Cuts at: 808 1419 2271 Size: 611 852 Fragments arranged by size: 852 611 328 218 105 49 37 28 27 16 SmaI CCC'GGG Cuts at: 0 183 210 389 422 990 1863 2271 Size: 183 27 179 33 568 873 408 Fragments arranged by size: 873 568 408 183 179 33 27 SmlI C'TyrA_G Cuts at: 0 724 1180 2271 Size: 724 456 1091 Fragments arranged by size: 1091 724 456 StuI AGG'CCT Cuts at: 0 547 668 976 2271 Size: 547 121 308 1295 Fragments arranged by size: 1295 547 308 121 StyI C'CwwG_G Cuts at: 0 227 1208 1646 1811 2271 Size: 227 981 438 165 460 Fragments arranged by size: 981 460 438 227 165 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 550 1809 2126 2271 Size: 550 1259 317 145 Fragments arranged by size: 1259 550 317 145 TatI w'GTAC_w Cuts at: 0 1631 2271 Size: 1631 640 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1045 1077 2163 2195 2271 Size: 1045 32 1086 32 76 Fragments arranged by size: 1086 1045 76 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1539 2271 Size: 1539 732 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 921 1020 1224 1506 2271 Size: 921 99 204 282 765 Fragments arranged by size: 921 765 282 204 99 XhoI C'TCGA_G Cuts at: 0 1180 2271 Size: 1180 1091 XmaI C'CCGG_G Cuts at: 0 181 208 387 420 988 1861 2271 Size: 181 27 179 33 568 873 410 Fragments arranged by size: 873 568 410 181 179 33 27 Enzymes that do cut: AflIII AleI AlfI AlwNI ApaI AscI AvaI BamHI BanI BanII BbeI BbsI BbvCI BclI BglI BlpI Bme1580I BmgBI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BsrBI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EarI EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI PasI PciI PflMI PfoI PpuMI PspOMI PvuII SacI SacII SapI SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI AloI ApaLI ApoI AseI AsiSI AvrII BaeI BcgI BciVI BfrBI BglII BmrI BmtI BplI BpmI BsiEI BsmI BspHI BspMI BsrDI BsrGI BstBI BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EciI Eco57MI EcoRI FalI HpaI KpnI MfeI MluI NdeI NheI NotI NsiI PacI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII SalI SanDI SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI XmnI ZraI