################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## Acc65I AccI AclI AflII AscI AseI AsnI Asp700I Asp718I AvrII BcgI BclI BfaI BfrI BlnI BsaBI BsiWI Bsp1407I BsrGI Bst1107I BstBI BstZ17I CpoI DraI EcoRV FbaI FseI HindIII HpaI KpnI MaeI MamI MfeI MseI MunI NcoI NdeI NheI NruI NspV PacI PmeI PshBI Psp1406I PvuI RsrII SalI SapI ScaI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI Tru9I XbaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AatII 2248 GACGTC GACGT^C AcsI 2544 GAATTC [GA]^AATT[CT] AgeI 1054 ACCGGT A^CCGGT AhdI 2044 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 3152 AGCGCT AGC^GCT ApoI 2544 GAATTC [GA]^AATT[CT] AspEI 2044 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2407 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 4137 CCTCAGC CCTCAGC(-5/-2) BspDI 2059 ATCGAT AT^CGAT BspHI 3662 TCATGA T^CATGA BsrDI 1371 CATTGC GCAATG(2/0) BssHII 80 GCGCGC G^CGCGC BstEII 1057 GGTGACC G^GT[ACGT]ACC BstPI 1057 GGTGACC G^GT[ACGT]ACC BstXI 3197 CCATTTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 2059 ATCGAT AT^CGAT Eam1105I 2044 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3152 AGCGCT AGC^GCT EcoO65I 1057 GGTGACC G^GT[ACGT]ACC EcoRI 2544 GAATTC G^AATTC EcoT22I 2282 ATGCAT ATGCA^T HincII 3598 GTTGAC GT[CT]^[GA]AC HindII 3598 GTTGAC GT[CT]^[GA]AC NsiI 2282 ATGCAT ATGCA^T PaeR7I 322 CTCGAG C^TCGAG PflFI 2407 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1054 ACCGGT A^CCGGT RcaI 3662 TCATGA T^CATGA SfiI 4146 GGCCCCCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Tth111I 2407 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 322 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### Alw44I 3030 GTGCAC G^TGCAC 3547 GTGCAC AocI 2067 CCTGAGG CC^T[ACGT]AGG 2274 CCTGAGG ApaLI 3030 GTGCAC G^TGCAC 3547 GTGCAC AviII 296 TGCGCA TGC^GCA 2591 TGCGCA BbrPI 1608 CACGTG CAC^GTG 4623 CACGTG BbsI 2431 GTCTTC GAAGAC(2/6) 3511 GTCTTC BciVI 2651 GTATCC GTATCC(6/5) 3554 GTATCC BglII 1736 AGATCT A^GATCT 2012 AGATCT BpuAI 2431 GTCTTC GAAGAC(2/6) 3511 GTCTTC BsmBI 3767 CGTCTC CGTCTC(1/5) 2665 GAGACG BsmI 814 GAATGC GAATGC(1/-1) 1477 GAATGC BspLU11I 2621 ACATGT A^CATGT 4481 ACATGT BssSI 4026 CACGAG CACGAG(-5/-1) 3349 CTCGTG Bsu36I 2067 CCTGAGG CC^T[ACGT]AGG 2274 CCTGAGG DraIII 1161 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG 1519 CACTGGGTG DrdI 2832 GACTCTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3444 GACCTGGATGTC EarI 3490 CTCTTC CTCTTC(1/4) 3502 CTCTTC Eco81I 2067 CCTGAGG CC^T[ACGT]AGG 2274 CCTGAGG EcoNI 750 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1947 CCTGGCCAAGG FspI 296 TGCGCA TGC^GCA 2591 TGCGCA Ksp632I 3490 CTCTTC CTCTTC(1/4) 3502 CTCTTC MluI 581 ACGCGT A^CGCGT 1471 ACGCGT NaeI 3837 GCCGGC GCC^GGC 4299 GCCGGC NgoMIV 3837 GCCGGC G^CCGGC 4299 GCCGGC NotI 88 GCGGCCGC GC^GGCCGC 169 GCGGCCGC NspI 2621 ACATGT [GA]CATG^[CT] 4481 ACATGT PmaCI 1608 CACGTG CAC^GTG 4623 CACGTG PmlI 1608 CACGTG CAC^GTG 4623 CACGTG PshAI 580 GACGCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4743 GACCGAGGTC PvuII 1443 CAGCTG CAG^CTG 2194 CAGCTG SacI 3411 GAGCTC GAGCT^C 4401 GAGCTC SbfI 405 CCTGCAGG CCTGCA^GG 1304 CCTGCAGG Sse8387I 405 CCTGCAGG CCTGCA^GG 1304 CCTGCAGG TfiI 2169 GAATC G^A[AT]TC 3209 GATTC Tsp509I 2545 AATT ^AATT 3823 AATT XcmI 2619 CCACATGTATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4703 CCACGATCCCGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 130 enzymes) # ######################################### AccII AccIII AciI AcyI AfaI AflIII AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BamHI BanI BanII BbeI BbvI BcnI BglI BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KspI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI RsaI SacII Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SfoI SmaI SmlI StuI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XhoII XmaCI XmaI