(Circular) (Six-base) MAPSORT of: pFN21AE9450.seq Check: 3873 from: 1 to: 9457 pFN21AE9450 9457 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2873 3119 3317 5874 2873 Size: 246 198 2557 6456 Fragments arranged by size: 6456 2557 246 198 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4257 7267 278 Size: 53 83 186 936 2721 3010 2468 Fragments arranged by size: 3010 2721 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6800 1937 Size: 4863 4594 Acc65I G'GTAC_C Cuts at: 6778 6778 Size: 9457 AclI AA'CG_TT Cuts at: 1563 7584 7957 1563 Size: 6021 373 3063 Fragments arranged by size: 6021 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5408 5783 5816 7520 8904 9172 1632 Size: 3776 375 33 1704 1384 268 1917 Fragments arranged by size: 3776 1917 1704 1384 375 268 33 AfeI AGC'GCT Cuts at: 5159 5159 Size: 9457 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9260 AflIII A'CryG_T Cuts at: 1203 2586 3476 3614 4626 4911 5850 6486 8356 Size: 1383 890 138 1012 285 939 636 1870 Cuts at: 8356 9333 1203 Size: 977 1327 Fragments arranged by size: 1870 1383 1327 1012 977 939 890 636 285 138 AgeI A'CCGG_T Cuts at: 3059 8288 3059 Size: 5229 4228 AhdI GACnn_n'nnGTC Cuts at: 4054 8186 4054 Size: 4132 5325 AleI CACnn'nnGTG Cuts at: 1166 2141 5042 6632 1166 Size: 975 2901 1590 3991 Fragments arranged by size: 3991 2901 1590 975 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2885 1184 Size: 1701 7756 AlwNI CAG_nnn'CTG Cuts at: 2431 2569 2983 3111 3199 3276 4009 6280 6499 Size: 138 414 128 88 77 733 2271 219 Cuts at: 6499 7200 8772 2431 Size: 701 1572 3116 Fragments arranged by size: 3116 2271 1572 733 701 414 219 138 128 88 77 ApaI G_GGCC'C Cuts at: 2033 2118 2124 2498 3350 3660 3689 4211 4713 Size: 85 6 374 852 310 29 522 502 Cuts at: 4713 5898 6013 6213 6369 2033 Size: 1185 115 200 156 5121 Fragments arranged by size: 5121 1185 852 522 502 374 310 200 156 115 85 29 6 ApaLI G'TGCA_C Cuts at: 5035 5552 7514 8670 5035 Size: 517 1962 1156 5822 Fragments arranged by size: 5822 1962 1156 517 ApoI r'AATT_y Cuts at: 1493 4549 6766 6992 7144 9253 1493 Size: 3056 2217 226 152 2109 1697 Fragments arranged by size: 3056 2217 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 8011 160 Size: 872 6979 1606 Fragments arranged by size: 6979 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9457 AvaI C'yCGr_G Cuts at: 1870 1943 2026 2117 2123 2327 2478 3537 3728 Size: 73 83 91 6 204 151 1059 191 Cuts at: 3728 3942 4706 5780 6155 6215 6329 6782 1870 Size: 214 764 1074 375 60 114 453 4545 Fragments arranged by size: 4545 1074 1059 764 453 375 214 204 191 151 114 91 83 73 60 6 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4662 4695 4662 Size: 33 9424 BamHI G'GATC_C Cuts at: 2040 5334 6325 6787 7154 2040 Size: 3294 991 462 367 4343 Fragments arranged by size: 4343 3294 991 462 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2269 2442 2780 3233 3713 Size: 325 217 627 482 173 338 453 480 Cuts at: 3713 4402 4487 4637 4738 5944 6220 6307 6477 Size: 689 85 150 101 1206 276 87 170 Cuts at: 6477 6704 6778 8233 618 Size: 227 74 1455 1842 Fragments arranged by size: 1842 1455 1206 689 627 482 480 453 338 325 276 227 217 173 170 150 101 87 85 74 BanII G_rGCy'C Cuts at: 1391 2033 2118 2124 2472 2498 3350 3660 3689 Size: 642 85 6 348 26 852 310 29 Cuts at: 3689 3735 3888 4211 4713 5184 5420 5642 5898 Size: 46 153 323 502 471 236 222 256 Cuts at: 5898 6013 6093 6213 6369 6410 6776 1391 Size: 115 80 120 156 41 366 4072 Fragments arranged by size: 4072 852 642 502 471 366 348 323 310 256 236 222 156 153 120 115 85 80 46 41 29 26 6 BbeI G_GCGC'C Cuts at: 2273 2446 2784 5948 6708 2273 Size: 173 338 3164 760 5022 Fragments arranged by size: 5022 3164 760 338 173 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4429 5509 928 Size: 971 2530 1080 4876 Fragments arranged by size: 4876 2530 1080 971 BbvCI CC'TCA_GC Cuts at: 6143 6143 Size: 9457 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7648 7682 1903 Size: 34 5711 34 3678 Fragments arranged by size: 5711 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4667 5570 7349 8559 4667 Size: 903 1779 1210 5565 Fragments arranged by size: 5565 1779 1210 903 BclI T'GATC_A Cuts at: 1548 1548 Size: 9457 BfrBI ATG'CAT Cuts at: 4289 4289 Size: 9457 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2655 4202 6158 Size: 107 122 71 631 605 983 1547 1956 Cuts at: 6158 8068 136 Size: 1910 1525 Fragments arranged by size: 1956 1910 1547 1525 983 631 605 122 107 71 BglII A'GATC_T Cuts at: 3741 4017 9452 3741 Size: 276 5435 3746 Fragments arranged by size: 5435 3746 276 BlpI GC'TnA_GC Cuts at: 3963 4808 6002 6874 3963 Size: 845 1194 872 6546 Fragments arranged by size: 6546 1194 872 845 Bme1580I G_kGCm'C Cuts at: 1790 2033 2118 2124 2280 2498 2778 3224 3350 Size: 243 85 6 156 218 280 446 126 Cuts at: 3350 3389 3563 3660 3689 4211 4490 4713 4743 Size: 39 174 97 29 522 279 223 30 Cuts at: 4743 5039 5556 5759 5898 6013 6213 6369 7518 Size: 296 517 203 139 115 200 156 1149 Cuts at: 7518 8674 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1149 522 517 446 296 280 279 243 223 218 203 200 174 156 156 139 126 115 97 85 39 30 29 6 BmgBI CAC'GTC Cuts at: 1318 5689 1318 Size: 4371 5086 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3218 3316 3535 Size: 416 53 83 399 24 1801 98 219 Cuts at: 3535 3557 3889 4402 7188 8146 442 Size: 22 332 513 2786 958 1753 Fragments arranged by size: 2786 1801 1753 958 513 416 399 332 219 98 83 53 24 22 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9457 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4006 4006 Size: 9457 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2831 2933 3088 3253 3277 3451 5285 5549 Size: 1592 102 155 165 24 174 1834 264 Cuts at: 5549 6320 6434 6668 6692 6728 8117 1239 Size: 771 114 234 24 36 1389 2579 Fragments arranged by size: 2579 1834 1592 1389 771 264 234 174 165 155 114 102 36 24 24 Bpu10I CC'TnA_GC Cuts at: 4151 6143 4151 Size: 1992 7465 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2731 5915 7582 8447 8745 2731 Size: 3184 1667 865 298 3443 Fragments arranged by size: 3443 3184 1667 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3527 3776 3994 8120 882 Size: 617 2028 249 218 4126 2219 Fragments arranged by size: 4126 2219 2028 617 249 218 BsaAI yAC'GTr Cuts at: 493 1204 3615 4912 6040 6630 7248 493 Size: 711 2411 1297 1128 590 618 2702 Fragments arranged by size: 2702 2411 1297 1128 711 618 590 BsaBI GATnn'nnATC Cuts at: 9451 9451 Size: 9457 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2080 2270 2443 2781 Size: 53 83 186 936 547 190 173 338 Cuts at: 2781 3590 4254 4749 5945 6578 6623 6705 7264 Size: 809 664 495 1196 633 45 82 559 Cuts at: 7264 7646 8281 275 Size: 382 635 1451 Fragments arranged by size: 1451 1196 936 809 664 635 633 559 547 495 382 338 190 186 173 83 82 53 45 BsaWI w'CCGG_w Cuts at: 3059 3810 4095 4755 5136 6504 6606 7890 8288 Size: 751 285 660 381 1368 102 1284 398 Cuts at: 8288 8562 8709 9120 3059 Size: 274 147 411 3396 Fragments arranged by size: 3396 1368 1284 751 660 411 398 381 285 274 147 102 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2495 2525 4601 4631 4766 Size: 30 165 30 1092 30 2076 30 135 Cuts at: 4766 4796 5471 5501 6080 6110 1178 Size: 30 675 30 579 30 4525 Fragments arranged by size: 4525 2076 1092 675 579 165 135 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2930 3026 3092 3145 4184 4205 4675 4678 Size: 1744 96 66 53 1039 21 470 3 Cuts at: 4678 4972 5630 5798 5801 6101 1186 Size: 294 658 168 3 300 4542 Fragments arranged by size: 4542 1744 1039 658 470 300 294 168 96 66 53 21 3 3 BseYI C'CCAG_C Cuts at: 2074 2457 2546 2954 3016 3046 3067 3717 3787 Size: 383 89 408 62 30 21 650 70 Cuts at: 3787 4197 4333 4803 5085 5175 5661 6200 6231 Size: 410 136 470 282 90 486 539 31 Cuts at: 6231 6270 6314 6356 6368 6544 8660 9156 2074 Size: 39 44 42 12 176 2116 496 2375 Fragments arranged by size: 2375 2116 650 539 496 486 470 410 408 383 282 176 136 90 89 70 62 44 42 39 31 30 21 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2165 3193 5042 5172 5846 5852 1163 Size: 573 429 1028 1849 130 674 6 4768 Fragments arranged by size: 4768 1849 1028 674 573 429 130 6 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2097 2178 2296 4542 5482 5771 Size: 275 186 96 81 118 2246 940 289 Cuts at: 5771 6907 7668 8696 1540 Size: 1136 761 1028 2301 Fragments arranged by size: 2301 2246 1136 1028 940 761 289 275 186 118 96 81 BsiHKAI G_wGCw'C Cuts at: 3104 3951 4497 5039 5094 5420 5469 5556 6410 Size: 847 546 542 55 326 49 87 854 Cuts at: 6410 7518 7603 8674 3104 Size: 1108 85 1071 3887 Fragments arranged by size: 3887 1108 1071 854 847 546 542 326 87 85 55 49 BsmI GAATG_Cn' Cuts at: 2825 3488 6968 7061 2825 Size: 663 3480 93 5221 Fragments arranged by size: 5221 3480 663 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4664 5778 4664 Size: 1114 8343 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2118 2124 2280 2472 2498 2778 Size: 399 243 85 6 156 192 26 280 Cuts at: 2778 3104 3224 3350 3389 3563 3660 3689 3735 Size: 326 120 126 39 174 97 29 46 Cuts at: 3735 3888 3951 4211 4490 4497 4713 4743 5039 Size: 153 63 260 279 7 216 30 296 Cuts at: 5039 5094 5184 5420 5469 5556 5642 5759 5898 Size: 55 90 236 49 87 86 117 139 Cuts at: 5898 6013 6093 6213 6369 6410 6776 7518 7603 Size: 115 80 120 156 41 366 742 85 Cuts at: 7603 8674 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 366 326 296 280 279 260 243 236 216 192 174 156 156 153 139 126 120 120 117 115 97 90 87 86 85 85 80 63 55 49 46 41 39 30 29 26 7 6 BspEI T'CCGG_A Cuts at: 4095 4755 5136 6606 9120 4095 Size: 660 381 1470 2514 4432 Fragments arranged by size: 4432 2514 1470 660 381 BspHI T'CATG_A Cuts at: 5667 7346 9076 5667 Size: 1679 1730 6048 Fragments arranged by size: 6048 1730 1679 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2873 3119 3317 4059 5305 5874 6812 844 Size: 2029 246 198 742 1246 569 938 3489 Fragments arranged by size: 3489 2029 1246 938 742 569 246 198 BsrBI CCG'CTC Cuts at: 1394 1823 5395 5487 6418 7344 1394 Size: 429 3572 92 931 926 3507 Fragments arranged by size: 3572 3507 931 926 429 92 BsrDI GCAATG_nn' Cuts at: 65 1248 3375 7952 8126 65 Size: 1183 2127 4577 174 1396 Fragments arranged by size: 4577 2127 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2597 3059 3702 4776 5607 5842 6304 Size: 110 787 462 643 1074 831 235 462 Cuts at: 6304 8101 8288 1700 Size: 1797 187 2869 Fragments arranged by size: 2869 1797 1074 831 787 643 462 462 235 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9457 BssHII G'CGCG_C Cuts at: 1925 2085 1925 Size: 160 9297 BssSI C'ACGA_G Cuts at: 5354 6031 7517 8529 5354 Size: 677 1486 1012 6282 Fragments arranged by size: 6282 1486 1012 677 BstAPI GCAn_nnn'nTGC Cuts at: 794 2431 3171 6227 6280 794 Size: 1637 740 3056 53 3971 Fragments arranged by size: 3971 3056 1637 740 53 BstBI TT'CG_AA Cuts at: 8261 8261 Size: 9457 BstEII G'GTnAC_C Cuts at: 1184 3062 1184 Size: 1878 7579 BstXI CCAn_nnnn'nTGG Cuts at: 5209 5209 Size: 9457 BstYI r'GATC_y Cuts at: 1972 2040 3741 4017 4146 5334 5502 5692 6325 Size: 68 1701 276 129 1188 168 190 633 Cuts at: 6325 6787 7154 7539 7556 9008 9094 9106 9452 Size: 462 367 385 17 1452 86 12 346 Cuts at: 9452 1972 Size: 1977 Fragments arranged by size: 1977 1701 1452 1188 633 462 385 367 346 276 190 168 129 86 68 17 12 Bsu36I CC'TnA_GG Cuts at: 4073 4280 4073 Size: 207 9250 BtgI C'CryG_G Cuts at: 513 1065 2172 2234 2514 2676 2695 3089 3283 Size: 552 1107 62 280 162 19 394 194 Cuts at: 3283 4383 4443 6051 6057 6616 513 Size: 1100 60 1608 6 559 3354 Fragments arranged by size: 3354 1608 1107 1100 559 552 394 280 194 162 62 60 19 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3856 5710 487 Size: 683 56 2630 1854 4234 Fragments arranged by size: 4234 2630 1854 683 56 BtsI GCAGTG_nn' Cuts at: 3176 3405 3563 3713 5739 6973 7767 7787 3176 Size: 229 158 150 2026 1234 794 20 4846 Fragments arranged by size: 4846 2026 1234 794 229 158 150 20 ClaI AT'CG_AT Cuts at: 1554 4065 1554 Size: 2511 6946 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9422 DraI TTT'AAA Cuts at: 6761 6761 Size: 9457 DraIII CAC_nnn'GTG Cuts at: 3171 3529 3171 Size: 358 9099 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4843 5455 8464 809 Size: 507 3527 612 3009 1802 Fragments arranged by size: 3527 3009 1802 612 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2094 2175 2190 Size: 54 1425 50 275 18 264 81 15 Cuts at: 2190 2262 2293 2595 3280 3391 3954 5046 5199 Size: 72 31 302 685 111 563 1092 153 Cuts at: 5199 5653 5768 5844 6054 6060 6904 7793 9422 Size: 454 115 76 210 6 844 889 1629 Cuts at: 9422 8 Size: 43 Fragments arranged by size: 1629 1425 1092 889 844 685 563 454 302 275 264 210 153 115 111 81 76 72 54 50 43 31 18 15 6 EagI C'GGCC_G Cuts at: 1537 1812 2094 2175 2293 5768 6904 1537 Size: 275 282 81 118 3475 1136 4090 Fragments arranged by size: 4090 3475 1136 282 275 118 81 EarI CTCTTCn'nnn_ Cuts at: 1353 5501 5513 7387 1353 Size: 4148 12 1874 3423 Fragments arranged by size: 4148 3423 1874 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3433 4681 5479 6237 6436 8044 Size: 1064 138 1844 1248 798 758 199 1608 Cuts at: 8044 8418 8564 387 Size: 374 146 1280 Fragments arranged by size: 1844 1608 1280 1248 1064 798 758 374 199 146 138 EcoICRI GAG'CTC Cuts at: 5418 6408 5418 Size: 990 8467 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2831 2933 3088 3253 3277 3451 5285 Size: 393 1199 102 155 165 24 174 1834 Cuts at: 5285 5408 5549 5783 5816 6320 6434 6668 6692 Size: 123 141 234 33 504 114 234 24 Cuts at: 6692 6728 7520 8117 8904 9172 1239 Size: 36 792 597 787 268 1524 Fragments arranged by size: 1834 1524 1199 792 787 597 504 393 268 234 234 174 165 155 141 123 114 102 36 33 24 24 EcoNI CCTnn'n_nnAGG Cuts at: 2759 3956 2759 Size: 1197 8260 EcoO109I rG'GnC_Cy Cuts at: 2030 2114 2495 2681 3315 3346 3347 3657 3685 Size: 84 381 186 634 31 1 310 28 Cuts at: 3685 3686 4048 4208 4276 4710 5294 5403 5895 Size: 1 362 160 68 434 584 109 492 Cuts at: 5895 6210 6353 6365 6496 6591 2030 Size: 315 143 12 131 95 4896 Fragments arranged by size: 4896 634 584 492 434 381 362 315 310 186 160 143 131 109 95 84 68 31 28 12 1 1 EcoRI G'AATT_C Cuts at: 4549 6766 7144 4549 Size: 2217 378 6862 Fragments arranged by size: 6862 2217 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4453 5974 4453 Size: 1521 7936 FspI TGC'GCA Cuts at: 2303 4598 7963 2303 Size: 2295 3365 3797 Fragments arranged by size: 3797 3365 2295 FspAI rTGC'GCAy Cuts at: 2303 2303 Size: 9457 HaeII r_GCGC'y Cuts at: 1678 2242 2273 2446 2784 3438 4815 5161 5447 Size: 564 31 173 338 654 1377 346 286 Cuts at: 5447 5948 6708 8604 1678 Size: 501 760 1896 2531 Fragments arranged by size: 2531 1896 1377 760 654 564 501 346 338 286 173 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2495 2527 3068 3100 3233 Size: 32 135 32 923 32 541 32 133 Cuts at: 3233 3265 4040 4072 4454 4486 4667 4699 4766 Size: 32 775 32 382 32 181 32 67 Cuts at: 4766 4798 5471 5503 8098 8130 8172 8204 1373 Size: 32 673 32 2595 32 42 32 2626 Fragments arranged by size: 2626 2595 923 775 673 541 382 181 135 133 67 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5605 6801 7047 669 Size: 988 281 3667 1196 246 3079 Fragments arranged by size: 3667 3079 1196 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9457 HpaI GTT'AAC Cuts at: 7047 7047 Size: 9457 KasI G'GCGC_C Cuts at: 2269 2442 2780 5944 6704 2269 Size: 173 338 3164 760 5022 Fragments arranged by size: 5022 3164 760 338 173 KpnI G_GTAC'C Cuts at: 6782 6782 Size: 9457 MfeI C'AATT_G Cuts at: 7056 7056 Size: 9457 MluI A'CGCG_T Cuts at: 2586 3476 9333 2586 Size: 890 5857 2710 Fragments arranged by size: 5857 2710 890 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 8571 8755 1305 Size: 7266 184 2007 Fragments arranged by size: 7266 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2264 3956 5201 10 Size: 54 1425 343 432 1692 1245 4266 Fragments arranged by size: 4266 1692 1425 1245 432 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2141 2258 3932 4910 5042 5120 5207 Size: 648 975 117 1674 978 132 78 87 Cuts at: 5207 5546 6632 7415 7774 7933 518 Size: 339 1086 783 359 159 2042 Fragments arranged by size: 2042 1674 1086 978 975 783 648 359 339 159 132 117 87 78 MspA1I CmG'CkG Cuts at: 1606 2053 2174 2236 2286 2577 2678 2697 2954 Size: 447 121 62 50 291 101 19 257 Cuts at: 2954 3177 3196 3450 3721 3791 3903 4201 4341 Size: 223 19 254 271 70 112 298 140 Cuts at: 4341 5303 5437 6204 6524 6655 6670 6718 6873 Size: 962 134 767 320 131 15 48 155 Cuts at: 6873 7550 8698 8943 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 962 767 677 447 320 298 291 271 257 254 245 223 155 140 134 131 121 112 101 70 62 50 48 19 19 15 NaeI GCC'GGC Cuts at: 5844 6306 5844 Size: 462 8995 NarI GG'CG_CC Cuts at: 2270 2443 2781 5945 6705 2270 Size: 173 338 3164 760 5022 Fragments arranged by size: 5022 3164 760 338 173 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 8905 NdeI CA'TA_TG Cuts at: 387 387 Size: 9457 NgoMIV G'CCGG_C Cuts at: 5842 6304 5842 Size: 462 8995 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9457 NotI GC'GGCC_GC Cuts at: 2094 2175 6904 2094 Size: 81 4729 4647 Fragments arranged by size: 4729 4647 81 NsiI A_TGCA'T Cuts at: 4291 4291 Size: 9457 NspI r_CATG'y Cuts at: 1132 1229 4630 6490 6815 8360 1132 Size: 97 3401 1860 325 1545 2229 Fragments arranged by size: 3401 2229 1860 1545 325 97 PasI CC'CwG_GG Cuts at: 1348 3457 4121 6594 1348 Size: 2109 664 2473 4211 Fragments arranged by size: 4211 2473 2109 664 PciI A'CATG_T Cuts at: 4626 6486 8356 4626 Size: 1860 1870 5727 Fragments arranged by size: 5727 1870 1860 PflMI CCAn_nnn'nTGG Cuts at: 1108 3230 3529 3569 4009 1108 Size: 2122 299 40 440 6556 Fragments arranged by size: 6556 2122 440 299 40 PfoI T'CCnGG_A Cuts at: 2210 2969 5577 6349 6492 2210 Size: 759 2608 772 143 5175 Fragments arranged by size: 5175 2608 772 759 143 PmeI GTTT'AAAC Cuts at: 6761 6761 Size: 9457 PmlI CAC'GTG Cuts at: 3615 6630 3615 Size: 3015 6442 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7526 7558 9039 9071 7526 Size: 32 1481 32 7912 Fragments arranged by size: 7912 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2681 4048 4276 5294 6353 6496 2681 Size: 1367 228 1018 1059 143 5642 Fragments arranged by size: 5642 1367 1059 1018 228 143 PshAI GACnn'nnGTC Cuts at: 1613 2589 6752 1613 Size: 976 4163 4318 Fragments arranged by size: 4318 4163 976 PsiI TTA'TAA Cuts at: 7027 7027 Size: 9457 PspOMI G'GGCC_C Cuts at: 2029 2114 2120 2494 3346 3656 3685 4207 4709 Size: 85 6 374 852 310 29 522 502 Cuts at: 4709 5894 6009 6209 6365 2029 Size: 1185 115 200 156 5121 Fragments arranged by size: 5121 1185 852 522 502 374 310 200 156 115 85 29 6 PspXI vC'TCGA_Gb Cuts at: 2327 2327 Size: 9457 PstI C_TGCA'G Cuts at: 830 2285 2415 2436 3033 3314 3555 4530 6516 Size: 1455 130 21 597 281 241 975 1986 Cuts at: 6516 6660 6809 830 Size: 144 149 3478 Fragments arranged by size: 3478 1986 1455 975 597 281 241 149 144 130 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9457 PvuII CAG'CTG Cuts at: 3450 4201 3450 Size: 751 8706 SacI G_AGCT'C Cuts at: 5420 6410 5420 Size: 990 8467 SacII CC_GC'GG Cuts at: 2175 2237 2679 2698 2175 Size: 62 442 19 8934 Fragments arranged by size: 8934 442 62 19 SalI G'TCGA_C Cuts at: 1936 6799 1936 Size: 4863 4594 SanDI GG'GwC_CC Cuts at: 2681 6353 2681 Size: 3672 5785 SbfI CC_TGCA'GG Cuts at: 2415 3314 6809 2415 Size: 899 3495 5063 Fragments arranged by size: 5063 3495 899 ScaI AGT'ACT Cuts at: 7705 9417 7705 Size: 1712 7745 SexAI A'CCwGG_T Cuts at: 2523 4293 4308 2523 Size: 1770 15 7672 Fragments arranged by size: 7672 1770 15 SfcI C'TryA_G Cuts at: 826 1045 2281 2411 2432 3029 3160 3310 3551 Size: 219 1236 130 21 597 131 150 241 Cuts at: 3551 3919 4039 4108 4498 4526 5701 6512 6656 Size: 368 120 69 390 28 1175 811 144 Cuts at: 6656 6805 7940 8621 8812 826 Size: 149 1135 681 191 1471 Fragments arranged by size: 1471 1236 1175 1135 811 681 597 390 368 241 219 191 150 149 144 131 130 120 69 28 21 SfiI GGCCn_nnn'nGGCC Cuts at: 6158 6158 Size: 9457 SfoI GGC'GCC Cuts at: 2271 2444 2782 5946 6706 2271 Size: 173 338 3164 760 5022 Fragments arranged by size: 5022 3164 760 338 173 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9457 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9457 SmaI CCC'GGG Cuts at: 1872 2028 2119 2480 3944 6331 6784 1872 Size: 156 91 361 1464 2387 453 4545 Fragments arranged by size: 4545 2387 1464 453 361 156 91 SmlI C'TyrA_G Cuts at: 820 1017 1943 2327 2710 5930 7561 8462 8724 Size: 197 926 384 383 3220 1631 901 262 Cuts at: 8724 820 Size: 1553 Fragments arranged by size: 3220 1631 1553 926 901 384 383 262 197 SnaBI TAC'GTA Cuts at: 493 7248 493 Size: 6755 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9457 SphI G_CATG'C Cuts at: 1132 6815 1132 Size: 5683 3774 SrfI GCCC'GGGC Cuts at: 2119 2119 Size: 9457 SspI AAT'ATT Cuts at: 5 52 7381 5 Size: 47 7329 2081 Fragments arranged by size: 7329 2081 47 StuI AGG'CCT Cuts at: 1517 2844 2942 3584 5102 1517 Size: 1327 98 642 1518 5872 Fragments arranged by size: 5872 1518 1327 642 98 StyI C'CwwG_G Cuts at: 513 1065 2265 3957 4218 4398 4605 5220 5811 Size: 552 1200 1692 261 180 207 615 591 Cuts at: 5811 6081 6896 513 Size: 270 815 3074 Fragments arranged by size: 3074 1692 1200 815 615 591 552 270 261 207 180 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3517 3731 5077 5687 6622 6764 7484 Size: 1255 1937 214 1346 610 935 142 720 Cuts at: 7484 7501 7654 7839 8284 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1937 1498 1346 1255 935 720 610 445 214 185 153 142 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6096 7703 9415 Size: 274 80 33 51 1443 4119 1607 1712 Cuts at: 9415 96 Size: 138 Fragments arranged by size: 4119 1712 1607 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3560 3592 9417 9449 3560 Size: 32 5825 32 3568 Fragments arranged by size: 5825 3568 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4415 1316 Size: 3099 6358 XbaI T'CTAG_A Cuts at: 6793 6793 Size: 9457 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4631 6715 4631 Size: 2084 7373 XhoI C'TCGA_G Cuts at: 1943 2327 1943 Size: 384 9073 XmaI C'CCGG_G Cuts at: 1870 2026 2117 2478 3942 6329 6782 1870 Size: 156 91 361 1464 2387 453 4545 Fragments arranged by size: 4545 2387 1464 453 361 156 91 XmnI GAAnn'nnTTC Cuts at: 7586 7586 Size: 9457 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4255 7265 276 Size: 53 83 186 936 2721 3010 2468 Fragments arranged by size: 3010 2721 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BsiWI BstZ17I EcoRV FseI NruI PacI PsrI RsrII SapI SwaI