(Circular) (Six-base) MAPSORT of: pFN21AE5722.seq Check: 8600 from: 1 to: 7315 pFN21AE5722 7315 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5125 278 Size: 53 83 186 936 3589 2468 Fragments arranged by size: 3589 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4321 4658 1937 Size: 2384 337 4594 Fragments arranged by size: 4594 2384 337 Acc65I G'GTAC_C Cuts at: 4636 4636 Size: 7315 AclI AA'CG_TT Cuts at: 1563 5442 5815 1563 Size: 3879 373 3063 Fragments arranged by size: 3879 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2225 2534 2602 3531 5378 6762 7030 1632 Size: 593 309 68 929 1847 1384 268 1917 Fragments arranged by size: 1917 1847 1384 929 593 309 268 68 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7118 AflIII A'CryG_T Cuts at: 1203 6214 7191 1203 Size: 5011 977 1327 Fragments arranged by size: 5011 1327 977 AgeI A'CCGG_T Cuts at: 6146 6146 Size: 7315 AhdI GACnn_n'nnGTC Cuts at: 2438 6044 2438 Size: 3606 3709 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7315 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3471 1184 Size: 2287 5028 AlwNI CAG_nnn'CTG Cuts at: 3458 5058 6630 3458 Size: 1600 1572 4143 Fragments arranged by size: 4143 1600 1572 ApaI G_GGCC'C Cuts at: 4068 4068 Size: 7315 ApaLI G'TGCA_C Cuts at: 2025 5372 6528 2025 Size: 3347 1156 2812 Fragments arranged by size: 3347 2812 1156 ApoI r'AATT_y Cuts at: 1493 2288 2317 3926 4103 4115 4490 4624 4850 Size: 795 29 1609 177 12 375 134 226 Cuts at: 4850 5002 7111 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1609 795 375 226 177 152 134 29 12 AseI AT'TA_AT Cuts at: 160 1032 5869 160 Size: 872 4837 1606 Fragments arranged by size: 4837 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7315 AvaI C'yCGr_G Cuts at: 1870 1943 4640 1870 Size: 73 2697 4545 Fragments arranged by size: 4545 2697 73 AvrII C'CTAG_G Cuts at: 3154 3154 Size: 7315 BamHI G'GATC_C Cuts at: 3590 4645 5012 3590 Size: 1055 367 5893 Fragments arranged by size: 5893 1055 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4636 6091 618 Size: 325 217 627 2849 1455 1842 Fragments arranged by size: 2849 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3387 3581 4068 4634 1391 Size: 1996 194 487 566 4072 Fragments arranged by size: 4072 1996 566 487 194 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2560 3842 3999 928 Size: 971 661 1282 157 4244 Fragments arranged by size: 4244 1282 971 661 157 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5506 5540 1903 Size: 34 3569 34 3678 Fragments arranged by size: 3678 3569 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5207 6417 5207 Size: 1210 6105 BclI T'GATC_A Cuts at: 1548 2080 1548 Size: 532 6783 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5926 136 Size: 107 122 71 631 605 4254 1525 Fragments arranged by size: 4254 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7310 7310 Size: 7315 BlpI GC'TnA_GC Cuts at: 4732 4732 Size: 7315 Bme1580I G_kGCm'C Cuts at: 1790 2029 3829 4068 5376 6532 1790 Size: 239 1800 239 1308 1156 2573 Fragments arranged by size: 2573 1800 1308 1156 239 239 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7315 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5046 6004 442 Size: 416 53 83 399 24 3629 958 1753 Fragments arranged by size: 3629 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7315 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3722 3722 Size: 7315 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2327 2676 2958 3371 3782 3882 5975 1239 Size: 1088 349 282 413 411 100 2093 2579 Fragments arranged by size: 2579 2093 1088 413 411 349 282 100 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5440 6305 6603 5440 Size: 865 298 6152 Fragments arranged by size: 6152 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2564 3390 4407 5978 882 Size: 617 1065 826 1017 1571 2219 Fragments arranged by size: 2219 1571 1065 1017 826 617 BsaAI yAC'GTr Cuts at: 493 1204 5106 493 Size: 711 3902 2702 Fragments arranged by size: 3902 2702 711 BsaBI GATnn'nnATC Cuts at: 7309 7309 Size: 7315 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5122 5504 6139 275 Size: 53 83 186 936 3589 382 635 1451 Fragments arranged by size: 3589 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2031 3034 3587 5748 6146 6420 6567 6978 2031 Size: 1003 553 2161 398 274 147 411 2368 Fragments arranged by size: 2368 2161 1003 553 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3016 3046 3269 3299 1178 Size: 30 165 30 1613 30 223 30 5194 Fragments arranged by size: 5194 1613 223 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2190 2709 3116 3408 3791 1186 Size: 1004 519 407 292 383 4710 Fragments arranged by size: 4710 1004 519 407 383 292 BseYI C'CCAG_C Cuts at: 2342 3794 4365 6518 7014 2342 Size: 1452 571 2153 496 2643 Fragments arranged by size: 2643 2153 1452 571 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2685 3260 3444 4301 1163 Size: 573 949 575 184 857 4177 Fragments arranged by size: 4177 949 857 575 573 184 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4049 4765 5526 6554 1540 Size: 275 186 2048 716 761 1028 2301 Fragments arranged by size: 2301 2048 1028 761 716 275 186 BsiHKAI G_wGCw'C Cuts at: 2029 3387 3581 4230 5376 5461 6532 2029 Size: 1358 194 649 1146 85 1071 2812 Fragments arranged by size: 2812 1358 1146 1071 649 194 85 BsmI GAATG_Cn' Cuts at: 4826 4919 4826 Size: 93 7222 BsmBI CGTCTCn'nnnn_ Cuts at: 3247 3247 Size: 7315 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2029 3387 3581 3829 4068 4230 4634 Size: 399 239 1358 194 248 239 162 404 Cuts at: 4634 5376 5461 6532 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1358 1071 742 404 399 248 239 239 194 162 85 BspEI T'CCGG_A Cuts at: 2031 3034 3587 6978 2031 Size: 1003 553 3391 2368 Fragments arranged by size: 3391 2368 1003 553 BspHI T'CATG_A Cuts at: 5204 6934 5204 Size: 1730 5585 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4670 844 Size: 3826 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5202 1394 Size: 429 3379 3507 Fragments arranged by size: 3507 3379 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4377 5810 5984 65 Size: 1183 3129 1433 174 1396 Fragments arranged by size: 3129 1433 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4048 4244 5959 6146 1700 Size: 110 2238 196 1715 187 2869 Fragments arranged by size: 2869 2238 1715 196 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7315 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7315 BssSI C'ACGA_G Cuts at: 2126 3443 5375 6387 2126 Size: 1317 1932 1012 3054 Fragments arranged by size: 3054 1932 1317 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3458 794 Size: 2664 4651 BstBI TT'CG_AA Cuts at: 3875 6119 3875 Size: 2244 5071 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7315 BstXI CCAn_nnnn'nTGG Cuts at: 3295 4405 3295 Size: 1110 6205 BstYI r'GATC_y Cuts at: 1972 3402 3590 4645 5012 5397 5414 6866 6952 Size: 1430 188 1055 367 385 17 1452 86 Cuts at: 6952 6964 7310 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1430 1055 385 367 346 188 86 17 12 BstZ17I GTA'TAC Cuts at: 4322 4322 Size: 7315 BtgI C'CryG_G Cuts at: 513 1065 2170 3430 513 Size: 552 1105 1260 4398 Fragments arranged by size: 4398 1260 1105 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6576 Fragments arranged by size: 6576 683 56 BtsI GCAGTG_nn' Cuts at: 4831 5625 5645 4831 Size: 794 20 6501 Fragments arranged by size: 6501 794 20 ClaI AT'CG_AT Cuts at: 1554 2053 1554 Size: 499 6816 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3643 3678 529 Size: 35 3079 35 4166 Fragments arranged by size: 4166 3079 35 35 DraI TTT'AAA Cuts at: 2826 3691 4619 2826 Size: 865 928 5522 Fragments arranged by size: 5522 928 865 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6322 809 Size: 507 5006 1802 Fragments arranged by size: 5006 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4046 4246 4762 Size: 54 1425 50 275 18 2216 200 516 Cuts at: 4762 5651 7280 8 Size: 889 1629 43 Fragments arranged by size: 2216 1629 1425 889 516 275 200 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4046 4762 1537 Size: 275 2234 716 4090 Fragments arranged by size: 4090 2234 716 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2775 4563 5245 1353 Size: 1422 1788 682 3423 Fragments arranged by size: 3423 1788 1422 682 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5902 6276 6422 387 Size: 1064 138 4313 374 146 1280 Fragments arranged by size: 4313 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3385 3579 3385 Size: 194 7121 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2225 2327 2534 2602 2676 2958 3371 Size: 393 593 102 207 68 74 282 413 Cuts at: 3371 3531 3782 3882 5378 5975 6762 7030 1239 Size: 160 251 100 1496 597 787 268 1524 Fragments arranged by size: 1524 1496 787 597 593 413 393 282 268 251 207 160 102 100 74 68 EcoNI CCTnn'n_nnAGG Cuts at: 3981 3981 Size: 7315 EcoO109I rG'GnC_Cy Cuts at: 2600 4064 2600 Size: 1464 5851 EcoRI G'AATT_C Cuts at: 2317 4103 4624 5002 2317 Size: 1786 521 378 4630 Fragments arranged by size: 4630 1786 521 378 EcoRV GAT'ATC Cuts at: 2057 4098 4219 2057 Size: 2041 121 5153 Fragments arranged by size: 5153 2041 121 FspI TGC'GCA Cuts at: 5821 5821 Size: 7315 HaeII r_GCGC'y Cuts at: 1678 6462 1678 Size: 4784 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2341 2373 2424 2456 3169 Size: 32 135 32 769 32 51 32 713 Cuts at: 3169 3201 3387 3419 4082 4114 5956 5988 6030 Size: 32 186 32 663 32 1842 32 42 Cuts at: 6030 6062 1373 Size: 32 2626 Fragments arranged by size: 2626 1842 769 713 663 186 135 51 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2233 3013 4659 4905 669 Size: 988 281 295 780 1646 246 3079 Fragments arranged by size: 3079 1646 988 780 295 281 246 HindIII A'AGCT_T Cuts at: 748 3118 4556 748 Size: 2370 1438 3507 Fragments arranged by size: 3507 2370 1438 HpaI GTT'AAC Cuts at: 4905 4905 Size: 7315 KpnI G_GTAC'C Cuts at: 4640 4640 Size: 7315 MfeI C'AATT_G Cuts at: 4914 4914 Size: 7315 MluI A'CGCG_T Cuts at: 7191 7191 Size: 7315 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3256 6429 6613 1305 Size: 1951 3173 184 2007 Fragments arranged by size: 3173 2007 1951 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5493 Fragments arranged by size: 5493 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2175 2852 5273 5632 5791 518 Size: 648 1009 677 2421 359 159 2042 Fragments arranged by size: 2421 2042 1009 677 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3455 4731 5408 6556 6801 1606 Size: 1849 1276 677 1148 245 2120 Fragments arranged by size: 2120 1849 1276 1148 677 245 NaeI GCC'GGC Cuts at: 4246 4246 Size: 7315 NcoI C'CATG_G Cuts at: 513 1065 2170 513 Size: 552 1105 5658 Fragments arranged by size: 5658 1105 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7315 NgoMIV G'CCGG_C Cuts at: 4244 4244 Size: 7315 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7315 NotI GC'GGCC_GC Cuts at: 4762 4762 Size: 7315 NspI r_CATG'y Cuts at: 1132 1229 2019 2135 3007 4673 6218 1132 Size: 97 790 116 872 1666 1545 2229 Fragments arranged by size: 2229 1666 1545 872 790 116 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7315 PciI A'CATG_T Cuts at: 6214 6214 Size: 7315 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7315 PfoI T'CCnGG_A Cuts at: 2653 2974 2653 Size: 321 6994 PmeI GTTT'AAAC Cuts at: 4619 4619 Size: 7315 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5384 5416 6897 6929 5384 Size: 32 1481 32 5770 Fragments arranged by size: 5770 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7315 PsiI TTA'TAA Cuts at: 4885 4885 Size: 7315 PspOMI G'GGCC_C Cuts at: 4064 4064 Size: 7315 PstI C_TGCA'G Cuts at: 830 2419 4667 830 Size: 1589 2248 3478 Fragments arranged by size: 3478 2248 1589 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7315 PvuII CAG'CTG Cuts at: 3455 3455 Size: 7315 SacI G_AGCT'C Cuts at: 3387 3581 3387 Size: 194 7121 SalI G'TCGA_C Cuts at: 1936 4657 1936 Size: 2721 4594 SapI GCTCTTCn'nnn_ Cuts at: 4563 4563 Size: 7315 SbfI CC_TGCA'GG Cuts at: 4667 4667 Size: 7315 ScaI AGT'ACT Cuts at: 5563 7275 5563 Size: 1712 5603 SfcI C'TryA_G Cuts at: 826 1045 2415 2755 4663 5798 6479 6670 826 Size: 219 1370 340 1908 1135 681 191 1471 Fragments arranged by size: 1908 1471 1370 1135 681 340 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7315 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7315 SmaI CCC'GGG Cuts at: 1872 4642 1872 Size: 2770 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5419 6320 6582 820 Size: 197 926 3476 901 262 1553 Fragments arranged by size: 3476 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5106 493 Size: 4613 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7315 SphI G_CATG'C Cuts at: 1132 4673 1132 Size: 3541 3774 SspI AAT'ATT Cuts at: 5 52 2796 4502 5239 5 Size: 47 2744 1706 737 2081 Fragments arranged by size: 2744 2081 1706 737 47 StuI AGG'CCT Cuts at: 1517 3031 3159 1517 Size: 1514 128 5673 Fragments arranged by size: 5673 1514 128 StyI C'CwwG_G Cuts at: 513 1065 2170 3154 4519 4754 513 Size: 552 1105 984 1365 235 3074 Fragments arranged by size: 3074 1365 1105 984 552 235 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2980 5342 5359 5512 5697 6142 325 Size: 1255 1400 2362 17 153 185 445 1498 Fragments arranged by size: 2362 1498 1400 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4251 5561 7273 Size: 274 80 33 51 1443 2274 1310 1712 Cuts at: 7273 96 Size: 138 Fragments arranged by size: 2274 1712 1443 1310 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3016 3048 3269 3301 3468 3500 3912 3944 4263 Size: 32 221 32 167 32 412 32 319 Cuts at: 4263 4295 7275 7307 3016 Size: 32 2980 32 3024 Fragments arranged by size: 3024 2980 412 319 221 167 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7315 XbaI T'CTAG_A Cuts at: 4651 4651 Size: 7315 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2651 2921 4204 2651 Size: 270 1283 5762 Fragments arranged by size: 5762 1283 270 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7315 XmaI C'CCGG_G Cuts at: 1870 4640 1870 Size: 2770 4545 XmnI GAAnn'nnTTC Cuts at: 5444 5444 Size: 7315 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5123 276 Size: 53 83 186 936 3589 2468 Fragments arranged by size: 3589 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI Bsu36I DraIII FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI