(Linear) (Six-base) MAPSORT of: KIEE5722.seq Check: 626 from: 1 to: 2610 KIEE5722.orf With 182 enzymes: SgfI * December 5, 2014 13:08 .. AccI GT'mk_AC Cuts at: 0 2316 2610 Size: 2316 294 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 220 529 597 1526 2610 Size: 220 309 68 929 1084 Fragments arranged by size: 1084 929 309 220 68 AhdI GACnn_n'nnGTC Cuts at: 0 433 2610 Size: 433 2177 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1466 2610 Size: 1466 1144 AlwNI CAG_nnn'CTG Cuts at: 0 1453 2610 Size: 1453 1157 ApaI G_GGCC'C Cuts at: 0 2063 2610 Size: 2063 547 ApaLI G'TGCA_C Cuts at: 0 20 2610 Size: 20 2590 ApoI r'AATT_y Cuts at: 0 283 312 1921 2098 2110 2485 2610 Size: 283 29 1609 177 12 375 125 Fragments arranged by size: 1609 375 283 177 125 29 12 AvrII C'CTAG_G Cuts at: 0 1149 2610 Size: 1149 1461 BamHI G'GATC_C Cuts at: 0 1585 2610 Size: 1585 1025 BanII G_rGCy'C Cuts at: 0 1382 1576 2063 2610 Size: 1382 194 487 547 Fragments arranged by size: 1382 547 487 194 BbsI GAAGACnn'nnnn_ Cuts at: 0 555 1837 1994 2610 Size: 555 1282 157 616 Fragments arranged by size: 1282 616 555 157 BclI T'GATC_A Cuts at: 0 75 2610 Size: 75 2535 Bme1580I G_kGCm'C Cuts at: 0 24 1824 2063 2610 Size: 24 1800 239 547 Fragments arranged by size: 1800 547 239 24 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1717 2610 Size: 1717 893 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 322 671 953 1366 1777 1877 2610 Size: 322 349 282 413 411 100 733 Fragments arranged by size: 733 413 411 349 322 282 100 BsaI GGTCTCn'nnnn_ Cuts at: 0 559 1385 2402 2610 Size: 559 826 1017 208 Fragments arranged by size: 1017 826 559 208 BsaWI w'CCGG_w Cuts at: 0 26 1029 1582 2610 Size: 26 1003 553 1028 Fragments arranged by size: 1028 1003 553 26 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1011 1041 1264 1294 2610 Size: 1011 30 223 30 1316 Fragments arranged by size: 1316 1011 223 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 185 704 1111 1403 1786 2610 Size: 185 519 407 292 383 824 Fragments arranged by size: 824 519 407 383 292 185 BseYI C'CCAG_C Cuts at: 0 337 1789 2360 2610 Size: 337 1452 571 250 Fragments arranged by size: 1452 571 337 250 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 680 1255 1439 2296 2610 Size: 680 575 184 857 314 Fragments arranged by size: 857 680 575 314 184 BsiEI CG_ry'CG Cuts at: 0 2044 2610 Size: 2044 566 BsiHKAI G_wGCw'C Cuts at: 0 24 1382 1576 2225 2610 Size: 24 1358 194 649 385 Fragments arranged by size: 1358 649 385 194 24 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1242 2610 Size: 1242 1368 Bsp1286I G_dGCh'C Cuts at: 0 24 1382 1576 1824 2063 2225 2610 Size: 24 1358 194 248 239 162 385 Fragments arranged by size: 1358 385 248 239 194 162 24 BspEI T'CCGG_A Cuts at: 0 26 1029 1582 2610 Size: 26 1003 553 1028 Fragments arranged by size: 1028 1003 553 26 BsrDI GCAATG_nn' Cuts at: 0 2372 2610 Size: 2372 238 BsrFI r'CCGG_y Cuts at: 0 2043 2239 2610 Size: 2043 196 371 Fragments arranged by size: 2043 371 196 BssSI C'ACGA_G Cuts at: 0 121 1438 2610 Size: 121 1317 1172 Fragments arranged by size: 1317 1172 121 BstAPI GCAn_nnn'nTGC Cuts at: 0 1453 2610 Size: 1453 1157 BstBI TT'CG_AA Cuts at: 0 1870 2610 Size: 1870 740 BstXI CCAn_nnnn'nTGG Cuts at: 0 1290 2400 2610 Size: 1290 1110 210 Fragments arranged by size: 1290 1110 210 BstYI r'GATC_y Cuts at: 0 1397 1585 2610 Size: 1397 188 1025 Fragments arranged by size: 1397 1025 188 BstZ17I GTA'TAC Cuts at: 0 2317 2610 Size: 2317 293 BtgI C'CryG_G Cuts at: 0 165 1425 2610 Size: 165 1260 1185 Fragments arranged by size: 1260 1185 165 ClaI AT'CG_AT Cuts at: 0 48 2610 Size: 48 2562 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1638 1673 2610 Size: 1638 35 937 Fragments arranged by size: 1638 937 35 DraI TTT'AAA Cuts at: 0 821 1686 2610 Size: 821 865 924 Fragments arranged by size: 924 865 821 EaeI y'GGCC_r Cuts at: 0 2041 2241 2610 Size: 2041 200 369 Fragments arranged by size: 2041 369 200 EagI C'GGCC_G Cuts at: 0 2041 2610 Size: 2041 569 EarI CTCTTCn'nnn_ Cuts at: 0 770 2558 2610 Size: 770 1788 52 Fragments arranged by size: 1788 770 52 EcoICRI GAG'CTC Cuts at: 0 1380 1574 2610 Size: 1380 194 1036 Fragments arranged by size: 1380 1036 194 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 220 322 529 597 671 953 1366 1526 Size: 220 102 207 68 74 282 413 160 Cuts at: 1526 1777 1877 2610 Size: 251 100 733 Fragments arranged by size: 733 413 282 251 220 207 160 102 100 74 68 EcoNI CCTnn'n_nnAGG Cuts at: 0 1976 2610 Size: 1976 634 EcoO109I rG'GnC_Cy Cuts at: 0 595 2059 2610 Size: 595 1464 551 Fragments arranged by size: 1464 595 551 EcoRI G'AATT_C Cuts at: 0 312 2098 2610 Size: 312 1786 512 Fragments arranged by size: 1786 512 312 EcoRV GAT'ATC Cuts at: 0 52 2093 2214 2610 Size: 52 2041 121 396 Fragments arranged by size: 2041 396 121 52 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 336 368 419 451 1164 1196 1382 1414 Size: 336 32 51 32 713 32 186 32 Cuts at: 1414 2077 2109 2610 Size: 663 32 501 Fragments arranged by size: 713 663 501 336 186 51 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 228 1008 2610 Size: 228 780 1602 Fragments arranged by size: 1602 780 228 HindIII A'AGCT_T Cuts at: 0 1113 2551 2610 Size: 1113 1438 59 Fragments arranged by size: 1438 1113 59 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1251 2610 Size: 1251 1359 MslI CAynn'nnrTG Cuts at: 0 170 847 2610 Size: 170 677 1763 Fragments arranged by size: 1763 677 170 MspA1I CmG'CkG Cuts at: 0 1450 2610 Size: 1450 1160 NaeI GCC'GGC Cuts at: 0 2241 2610 Size: 2241 369 NcoI C'CATG_G Cuts at: 0 165 2610 Size: 165 2445 NgoMIV G'CCGG_C Cuts at: 0 2239 2610 Size: 2239 371 NspI r_CATG'y Cuts at: 0 14 130 1002 2610 Size: 14 116 872 1608 Fragments arranged by size: 1608 872 116 14 PfoI T'CCnGG_A Cuts at: 0 648 969 2610 Size: 648 321 1641 Fragments arranged by size: 1641 648 321 PspOMI G'GGCC_C Cuts at: 0 2059 2610 Size: 2059 551 PstI C_TGCA'G Cuts at: 0 414 2610 Size: 414 2196 PvuII CAG'CTG Cuts at: 0 1450 2610 Size: 1450 1160 SacI G_AGCT'C Cuts at: 0 1382 1576 2610 Size: 1382 194 1034 Fragments arranged by size: 1382 1034 194 SapI GCTCTTCn'nnn_ Cuts at: 0 2558 2610 Size: 2558 52 SfcI C'TryA_G Cuts at: 0 410 750 2610 Size: 410 340 1860 Fragments arranged by size: 1860 410 340 SspI AAT'ATT Cuts at: 0 791 2497 2610 Size: 791 1706 113 Fragments arranged by size: 1706 791 113 StuI AGG'CCT Cuts at: 0 1026 1154 2610 Size: 1026 128 1456 Fragments arranged by size: 1456 1026 128 StyI C'CwwG_G Cuts at: 0 165 1149 2514 2610 Size: 165 984 1365 96 Fragments arranged by size: 1365 984 165 96 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 975 2610 Size: 975 1635 TatI w'GTAC_w Cuts at: 0 2246 2610 Size: 2246 364 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1011 1043 1264 1296 1463 1495 1907 1939 Size: 1011 32 221 32 167 32 412 32 Cuts at: 1939 2258 2290 2610 Size: 319 32 320 Fragments arranged by size: 1011 412 320 319 221 167 32 32 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 646 916 2199 2610 Size: 646 270 1283 411 Fragments arranged by size: 1283 646 411 270 Enzymes that do cut: AccI AcuI AhdI AlfI AlwNI ApaI ApaLI ApoI AvrII BamHI BanII BbsI BclI Bme1580I BplI BpmI BsaI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI ClaI CspCI DraI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV Hin4I HincII HindIII MmeI MslI MspA1I NaeI NcoI NgoMIV NspI PfoI PspOMI PstI PvuII SacI SapI SfcI SspI StuI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AleI AloI AscI AseI AsiSI AvaI BaeI BanI BbeI BbvCI BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmrI BmtI Bpu10I BpuEI BsaAI BsaBI BsaHI BsiWI BsmI BspHI BspMI BsrBI BsrGI BssHII BstEII Bsu36I BtgZI BtsI DraIII DrdI EciI FalI FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI MscI NarI NdeI NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI