################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII AccIII AclI AflII AgeI Alw44I ApaLI AscI AseI AsnI AviII AvrII BamHI BbeI BcgI BciVI BclI BfrI BlnI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I ClaI CpoI DraI EagI EclXI Eco52I EcoRV EcoT22I FbaI FseI FspI HpaI KasI MamI MroI MseI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I XbaI XcmI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 785 GGTACC G^GTACC AcyI 1000 GACGCC G[GA]^CG[CT]C AhdI 2261 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 1359 AGCGCT AGC^GCT Asp700I 1896 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 785 GGTACC G^GTACC AspEI 2261 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1975 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 93 CACGTG CAC^GTG BbvCI 516 CCTCAGC CCTCAGC(-5/-2) BfaI 37 CTAG C^TAG BglII 1529 AGATCT A^GATCT BlpI 948 GCTCAGC GC^T[ACGT]AGC Bpu1102I 948 GCTCAGC GC^T[ACGT]AGC BsaAI 93 CACGTG [CT]AC^GT[GA] BsaHI 1000 GACGCC G[GA]^CG[CT]C BspLU11I 1502 ACATGT A^CATGT BsrI 1460 ACTGG ACTGG(1/-1) BssSI 1324 CACGAG CACGAG(-5/-1) CelII 948 GCTCAGC GC^T[ACGT]AGC Eam1105I 2261 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1359 AGCGCT AGC^GCT EcoRI 1132 GAATTC G^AATTC Hin1I 1000 GACGCC G[GA]^CG[CT]C HincII 1981 GTCGAC GT[CT]^[GA]AC HindII 1981 GTCGAC GT[CT]^[GA]AC HindIII 2094 AAGCTT A^AGCTT KpnI 785 GGTACC GGTAC^C MaeI 37 CTAG C^TAG MfeI 290 CAATTG C^AATTG MluI 1289 ACGCGT A^CGCGT MslI 880 CACCCGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 290 CAATTG C^AATTG NspI 1502 ACATGT [GA]CATG^[CT] PaeR7I 1064 CTCGAG C^TCGAG PflFI 1975 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 93 CACGTG CAC^GTG PmlI 93 CACGTG CAC^GTG SalI 1981 GTCGAC G^TCGAC Tth111I 1975 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1064 CTCGAG C^TCGAG XmnI 1896 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccI 76 GTCTAC GT^[AC][GT]AC 1981 GTCGAC AcsI 172 AAATTT [GA]^AATT[CT] 1132 GAATTC AflIII 1289 ACGCGT A^C[GA][CT]GT 1502 ACATGT AlwI 2209 GATCC GGATC(4/5) 212 GGATC ApoI 172 AAATTT [GA]^AATT[CT] 1132 GAATTC AspHI 403 GAGCAC G[AT]GC[AT]^C 2387 GTGCTC BalI 1794 TGGCCA TGG^CCA 1808 TGGCCA BbsI 1278 GAAGAC GAAGAC(2/6) 1972 GAAGAC BglI 3 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 614 GCCTCGAAGGC BpuAI 1278 GAAGAC GAAGAC(2/6) 1972 GAAGAC BsgI 43 GTGCAG GTGCAG(16/14) 634 GTGCAG BsiHKAI 403 GAGCAC G[AT]GC[AT]^C 2387 GTGCTC BsrFI 125 ACCGGC [GA]^CCGG[CT] 2485 ACCGGC BstEII 430 GGTGACC G^GT[ACGT]ACC 1329 GGTGACC BstPI 430 GGTGACC G^GT[ACGT]ACC 1329 GGTGACC BstYI 1529 AGATCT [GA]^GATC[CT] 2208 AGATCC Cfr10I 125 ACCGGC [GA]^CCGG[CT] 2485 ACCGGC DraIII 1324 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG 2342 CACAGTGTG DrdI 265 GACGTGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 448 GACCCCATGGTC EcoNI 1482 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1863 CCTGCCAGAGG EcoO65I 430 GGTGACC G^GT[ACGT]ACC 1329 GGTGACC HaeII 128 GGCGCT [GA]GCGC^[CT] 1359 AGCGCT KspI 7 CCGCGG CCGC^GG 1570 CCGCGG MflI 1529 AGATCT [GA]^GATC[CT] 2208 AGATCC MluNI 1794 TGGCCA TGG^CCA 1808 TGGCCA MscI 1794 TGGCCA TGG^CCA 1808 TGGCCA PflMI 859 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2073 CCAAGACGTGG SacII 7 CCGCGG CCGC^GG 1570 CCGCGG SmaI 882 CCCGGG CCC^GGG 1715 CCCGGG SmlI 1064 CTCGAG C^T[CT][GA]AG 1203 CTTGAG Van91I 859 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2073 CCAAGACGTGG XhoII 1529 AGATCT [GA]^GATC[CT] 2208 AGATCC XmaCI 882 CCCGGG C^CCGGG 1715 CCCGGG XmaI 882 CCCGGG C^CCGGG 1715 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI AocI ApaI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BssKI BstF5I BstNI BstUI BstXI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I