(Circular) (Six-base) MAPSORT of: pFN21AE6734.seq Check: 6538 from: 1 to: 7438 pFN21AE6734 7438 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2524 2524 Size: 7438 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5248 278 Size: 53 83 186 936 3712 2468 Fragments arranged by size: 3712 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2082 3987 4781 1937 Size: 145 1905 794 4594 Fragments arranged by size: 4594 1905 794 145 Acc65I G'GTAC_C Cuts at: 2790 4759 2790 Size: 1969 5469 AclI AA'CG_TT Cuts at: 1563 5565 5938 1563 Size: 4002 373 3063 Fragments arranged by size: 4002 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2233 2685 3203 3486 4075 4574 5501 6885 Size: 601 452 518 283 589 499 927 1384 Cuts at: 6885 7153 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 927 601 589 518 499 452 283 268 AfeI AGC'GCT Cuts at: 3366 3366 Size: 7438 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7241 AflIII A'CryG_T Cuts at: 1203 3294 3507 6337 7314 1203 Size: 2091 213 2830 977 1327 Fragments arranged by size: 2830 2091 1327 977 213 AgeI A'CCGG_T Cuts at: 6269 6269 Size: 7438 AhdI GACnn_n'nnGTC Cuts at: 4271 6167 4271 Size: 1896 5542 AleI CACnn'nnGTG Cuts at: 1166 2889 1166 Size: 1723 5715 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7438 AlwNI CAG_nnn'CTG Cuts at: 2870 4217 4532 4659 4678 5181 6753 2870 Size: 1347 315 127 19 503 1572 3555 Fragments arranged by size: 3555 1572 1347 503 315 127 19 ApaI G_GGCC'C Cuts at: 2815 3256 3401 3659 4061 2815 Size: 441 145 258 402 6192 Fragments arranged by size: 6192 441 402 258 145 ApaLI G'TGCA_C Cuts at: 5495 6651 5495 Size: 1156 6282 ApoI r'AATT_y Cuts at: 1493 2177 3137 4747 4973 5125 7234 1493 Size: 684 960 1610 226 152 2109 1697 Fragments arranged by size: 2109 1697 1610 960 684 226 152 AseI AT'TA_AT Cuts at: 160 1032 5992 160 Size: 872 4960 1606 Fragments arranged by size: 4960 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7438 AvaI C'yCGr_G Cuts at: 1870 1943 2706 2887 3069 3442 3720 4763 1870 Size: 73 763 181 182 373 278 1043 4545 Fragments arranged by size: 4545 1043 763 373 278 182 181 73 BamHI G'GATC_C Cuts at: 4768 5135 4768 Size: 367 7071 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2062 2790 2891 2992 4759 Size: 325 217 627 275 728 101 101 1767 Cuts at: 4759 6214 618 Size: 1455 1842 Fragments arranged by size: 1842 1767 1455 728 627 325 275 217 101 101 BanII G_rGCy'C Cuts at: 1391 2815 2850 3256 3401 3659 3744 4061 4189 Size: 1424 35 406 145 258 85 317 128 Cuts at: 4189 4690 4757 1391 Size: 501 67 4072 Fragments arranged by size: 4072 1424 501 406 317 258 145 128 85 67 35 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3290 3984 928 Size: 971 1391 694 4382 Fragments arranged by size: 4382 1391 971 694 BbvCI CC'TCA_GC Cuts at: 2522 2522 Size: 7438 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5629 5663 1903 Size: 34 50 34 3608 34 3678 Fragments arranged by size: 3678 3608 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5330 6540 5330 Size: 1210 6228 BclI T'GATC_A Cuts at: 1548 1548 Size: 7438 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2014 2625 6049 Size: 107 122 71 631 605 342 611 3424 Cuts at: 6049 136 Size: 1525 Fragments arranged by size: 3424 1525 631 611 605 342 122 107 71 BglII A'GATC_T Cuts at: 3534 7433 3534 Size: 3899 3539 BlpI GC'TnA_GC Cuts at: 2954 4855 2954 Size: 1901 5537 Bme1580I G_kGCm'C Cuts at: 1790 2067 2815 2896 2997 3256 3401 3659 4061 Size: 277 748 81 101 259 145 258 402 Cuts at: 4061 5499 6655 1790 Size: 1438 1156 2573 Fragments arranged by size: 2573 1438 1156 748 402 277 259 258 145 101 81 BmgBI CAC'GTC Cuts at: 1318 2047 2272 4084 1318 Size: 729 225 1812 4672 Fragments arranged by size: 4672 1812 729 225 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5169 6127 442 Size: 416 53 83 399 24 3752 958 1753 Fragments arranged by size: 3752 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7438 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3060 3553 3060 Size: 493 6945 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2141 2486 2750 3389 3635 3699 3812 6098 Size: 902 345 264 639 246 64 113 2286 Cuts at: 6098 1239 Size: 2579 Fragments arranged by size: 2579 2286 902 639 345 264 246 113 64 Bpu10I CC'TnA_GC Cuts at: 2522 4013 4679 2522 Size: 1491 666 5281 Fragments arranged by size: 5281 1491 666 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3229 5563 6428 6726 3229 Size: 2334 865 298 3941 Fragments arranged by size: 3941 2334 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3031 3295 4163 4258 6101 882 Size: 617 1532 264 868 95 1843 2219 Fragments arranged by size: 2219 1843 1532 868 617 264 95 BsaAI yAC'GTr Cuts at: 493 1204 2100 5229 493 Size: 711 896 3129 2702 Fragments arranged by size: 3129 2702 896 711 BsaBI GATnn'nnATC Cuts at: 7432 7432 Size: 7438 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3006 5245 5627 6262 Size: 53 83 186 936 1473 2239 382 635 Cuts at: 6262 275 Size: 1451 Fragments arranged by size: 2239 1473 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5871 6269 6543 6690 7101 5871 Size: 398 274 147 411 6208 Fragments arranged by size: 6208 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3104 3134 1178 Size: 30 165 30 1701 30 5482 Fragments arranged by size: 5482 1701 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2420 2606 2687 3036 3545 3569 3890 4388 Size: 1234 186 81 349 509 24 321 498 Cuts at: 4388 1186 Size: 4236 Fragments arranged by size: 4236 1234 509 498 349 321 186 81 24 BseYI C'CCAG_C Cuts at: 2601 2808 3339 3349 3563 4257 6641 7137 2601 Size: 207 531 10 214 694 2384 496 2902 Fragments arranged by size: 2902 2384 694 531 496 214 207 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2069 2660 1163 Size: 573 333 591 5941 Fragments arranged by size: 5941 591 573 333 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4888 5649 6677 1540 Size: 275 186 2887 761 1028 2301 Fragments arranged by size: 2887 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2412 4396 5499 5584 6655 2412 Size: 1984 1103 85 1071 3195 Fragments arranged by size: 3195 1984 1103 1071 85 BsmI GAATG_Cn' Cuts at: 4949 5042 4949 Size: 93 7345 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2412 2815 2850 2896 2997 3256 Size: 399 277 345 403 35 46 101 259 Cuts at: 3256 3401 3659 3744 4061 4189 4396 4690 4757 Size: 145 258 85 317 128 207 294 67 Cuts at: 4757 5499 5584 6655 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 403 399 345 317 294 277 259 258 207 145 128 101 85 85 67 46 35 BspEI T'CCGG_A Cuts at: 7101 7101 Size: 7438 BspHI T'CATG_A Cuts at: 5327 7057 5327 Size: 1730 5708 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2524 3090 3876 4793 844 Size: 1680 566 786 917 3489 Fragments arranged by size: 3489 1680 917 786 566 BsrBI CCG'CTC Cuts at: 1394 1823 3619 3625 3631 4327 4683 5325 1394 Size: 429 1796 6 6 696 356 642 3507 Fragments arranged by size: 3507 1796 696 642 429 356 6 6 BsrDI GCAATG_nn' Cuts at: 65 1248 5933 6107 65 Size: 1183 4685 174 1396 Fragments arranged by size: 4685 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2130 4490 4735 6082 6269 1700 Size: 110 320 2360 245 1347 187 2869 Fragments arranged by size: 2869 2360 1347 320 245 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7438 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7438 BssSI C'ACGA_G Cuts at: 3329 5498 6510 3329 Size: 2169 1012 4257 Fragments arranged by size: 4257 2169 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7438 BstBI TT'CG_AA Cuts at: 6242 6242 Size: 7438 BstEII G'GTnAC_C Cuts at: 1184 2435 3334 1184 Size: 1251 899 5288 Fragments arranged by size: 5288 1251 899 BstXI CCAn_nnnn'nTGG Cuts at: 2464 3313 4438 2464 Size: 849 1125 5464 Fragments arranged by size: 5464 1125 849 BstYI r'GATC_y Cuts at: 1972 3534 4213 4768 5135 5520 5537 6989 7075 Size: 1562 679 555 367 385 17 1452 86 Cuts at: 7075 7087 7433 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1562 1452 679 555 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2382 3488 3924 3969 4001 2382 Size: 1106 436 45 32 5819 Fragments arranged by size: 5819 1106 436 45 32 BtgI C'CryG_G Cuts at: 513 1065 2012 2184 2457 3393 3452 3575 4481 Size: 552 947 172 273 936 59 123 906 Cuts at: 4481 4575 513 Size: 94 3376 Fragments arranged by size: 3376 947 936 906 552 273 172 123 94 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3115 487 Size: 683 56 1889 4810 Fragments arranged by size: 4810 1889 683 56 BtsI GCAGTG_nn' Cuts at: 2583 4660 4954 5748 5768 2583 Size: 2077 294 794 20 4253 Fragments arranged by size: 4253 2077 794 294 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7438 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3250 3285 529 Size: 35 2686 35 4682 Fragments arranged by size: 4682 2686 35 35 DraI TTT'AAA Cuts at: 4742 4742 Size: 7438 DraIII CAC_nnn'GTG Cuts at: 3334 4352 3334 Size: 1018 6420 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2276 2459 6445 809 Size: 507 960 183 3986 1802 Fragments arranged by size: 3986 1802 960 507 183 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3258 3799 3813 Size: 54 1425 50 275 18 1428 541 14 Cuts at: 3813 4123 4572 4578 4885 5774 7403 8 Size: 310 449 6 307 889 1629 43 Fragments arranged by size: 1629 1428 1425 889 541 449 310 307 275 54 50 43 18 14 6 EagI C'GGCC_G Cuts at: 1537 1812 4885 1537 Size: 275 3073 4090 Fragments arranged by size: 4090 3073 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2252 2664 3111 4315 5368 1353 Size: 899 412 447 1204 1053 3423 Fragments arranged by size: 3423 1204 1053 899 447 412 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6025 6399 6545 387 Size: 1064 138 4436 374 146 1280 Fragments arranged by size: 4436 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2141 2233 2486 2685 2750 3203 3389 Size: 393 509 92 253 199 65 453 186 Cuts at: 3389 3486 3635 3699 3812 4075 4574 5501 6098 Size: 97 149 64 113 263 499 927 597 Cuts at: 6098 6885 7153 1239 Size: 787 268 1524 Fragments arranged by size: 1524 927 787 597 509 499 453 393 268 263 253 199 186 149 113 97 92 65 64 EcoNI CCTnn'n_nnAGG Cuts at: 3491 3872 3491 Size: 381 7057 EcoO109I rG'GnC_Cy Cuts at: 2203 2314 2386 2605 2812 2867 3253 3398 3655 Size: 111 72 219 207 55 386 145 257 Cuts at: 3655 3656 4057 4058 4118 4675 2203 Size: 1 401 1 60 557 4966 Fragments arranged by size: 4966 557 401 386 257 219 207 145 111 72 60 55 1 1 EcoRI G'AATT_C Cuts at: 3137 4747 5125 3137 Size: 1610 378 5450 Fragments arranged by size: 5450 1610 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2263 2263 Size: 7438 FspI TGC'GCA Cuts at: 5944 5944 Size: 7438 HaeII r_GCGC'y Cuts at: 1678 2137 3368 6585 1678 Size: 459 1231 3217 2531 Fragments arranged by size: 3217 2531 1231 459 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2429 2461 3052 3084 4198 Size: 32 135 32 857 32 591 32 1114 Cuts at: 4198 4205 4230 4237 4257 4289 6079 6111 6153 Size: 7 25 7 20 32 1790 32 42 Cuts at: 6153 6185 1373 Size: 32 2626 Fragments arranged by size: 2626 1790 1114 857 591 135 42 32 32 32 32 32 32 32 25 20 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 3988 4782 5028 669 Size: 988 281 2050 794 246 3079 Fragments arranged by size: 3079 2050 988 794 281 246 HindIII A'AGCT_T Cuts at: 748 4099 748 Size: 3351 4087 HpaI GTT'AAC Cuts at: 5028 5028 Size: 7438 KpnI G_GTAC'C Cuts at: 2794 4763 2794 Size: 1969 5469 MfeI C'AATT_G Cuts at: 2295 5037 2295 Size: 2742 4696 MluI A'CGCG_T Cuts at: 3294 7314 3294 Size: 4020 3418 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2151 3579 4026 4455 4740 6552 6736 1305 Size: 846 1428 447 429 285 1812 184 2007 Fragments arranged by size: 2007 1812 1428 846 447 429 285 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3801 3815 10 Size: 54 1425 343 1969 14 3633 Fragments arranged by size: 3633 1969 1425 343 54 14 MslI CAynn'nnrTG Cuts at: 518 1166 2889 5396 5755 5914 518 Size: 648 1723 2507 359 159 2042 Fragments arranged by size: 2507 2042 1723 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2014 2109 2215 2326 3577 3772 4523 4854 Size: 408 95 106 111 1251 195 751 331 Cuts at: 4854 5531 6679 6924 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1251 1148 751 677 408 331 245 195 111 106 95 NcoI C'CATG_G Cuts at: 513 1065 2184 2457 3452 4481 513 Size: 552 1119 273 995 1029 3470 Fragments arranged by size: 3470 1119 1029 995 552 273 NdeI CA'TA_TG Cuts at: 387 387 Size: 7438 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7438 NotI GC'GGCC_GC Cuts at: 4885 4885 Size: 7438 NspI r_CATG'y Cuts at: 1132 1229 3511 4796 6341 1132 Size: 97 2282 1285 1545 2229 Fragments arranged by size: 2282 2229 1545 1285 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7438 PciI A'CATG_T Cuts at: 3507 6337 3507 Size: 2830 4608 PflMI CCAn_nnn'nTGG Cuts at: 1108 2870 4084 1108 Size: 1762 1214 4462 Fragments arranged by size: 4462 1762 1214 PfoI T'CCnGG_A Cuts at: 2265 2463 4614 4638 2265 Size: 198 2151 24 5065 Fragments arranged by size: 5065 2151 198 24 PmeI GTTT'AAAC Cuts at: 4742 4742 Size: 7438 PmlI CAC'GTG Cuts at: 2100 2100 Size: 7438 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5507 5539 7020 7052 5507 Size: 32 1481 32 5893 Fragments arranged by size: 5893 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2203 2386 2605 4675 2203 Size: 183 219 2070 4966 Fragments arranged by size: 4966 2070 219 183 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7438 PsiI TTA'TAA Cuts at: 5008 5008 Size: 7438 PspOMI G'GGCC_C Cuts at: 2811 3252 3397 3655 4057 2811 Size: 441 145 258 402 6192 Fragments arranged by size: 6192 441 402 258 145 PspXI vC'TCGA_Gb Cuts at: 3069 3069 Size: 7438 PstI C_TGCA'G Cuts at: 830 2364 2661 4184 4528 4790 830 Size: 1534 297 1523 344 262 3478 Fragments arranged by size: 3478 1534 1523 344 297 262 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7438 PvuII CAG'CTG Cuts at: 2326 3772 4523 2326 Size: 1446 751 5241 Fragments arranged by size: 5241 1446 751 SacII CC_GC'GG Cuts at: 2015 3578 2015 Size: 1563 5875 SalI G'TCGA_C Cuts at: 1936 3986 4780 1936 Size: 2050 794 4594 Fragments arranged by size: 4594 2050 794 SanDI GG'GwC_CC Cuts at: 2605 2605 Size: 7438 SbfI CC_TGCA'GG Cuts at: 4790 4790 Size: 7438 ScaI AGT'ACT Cuts at: 5686 7398 5686 Size: 1712 5726 SfcI C'TryA_G Cuts at: 826 1045 2137 2360 2657 4180 4524 4786 5921 Size: 219 1092 223 297 1523 344 262 1135 Cuts at: 5921 6602 6793 826 Size: 681 191 1471 Fragments arranged by size: 1523 1471 1135 1092 681 344 297 262 223 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7438 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7438 SmaI CCC'GGG Cuts at: 1872 2889 3722 4765 1872 Size: 1017 833 1043 4545 Fragments arranged by size: 4545 1043 1017 833 SmlI C'TyrA_G Cuts at: 820 1017 1943 3069 3208 5542 6443 6705 820 Size: 197 926 1126 139 2334 901 262 1553 Fragments arranged by size: 2334 1553 1126 926 901 262 197 139 SnaBI TAC'GTA Cuts at: 493 5229 493 Size: 4736 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7438 SphI G_CATG'C Cuts at: 1132 4796 1132 Size: 3664 3774 SspI AAT'ATT Cuts at: 5 52 5362 5 Size: 47 5310 2081 Fragments arranged by size: 5310 2081 47 StuI AGG'CCT Cuts at: 1517 3075 3180 4018 1517 Size: 1558 105 838 4937 Fragments arranged by size: 4937 1558 838 105 StyI C'CwwG_G Cuts at: 513 1065 2184 2457 3452 3816 4481 4877 513 Size: 552 1119 273 995 364 665 396 3074 Fragments arranged by size: 3074 1119 995 665 552 396 364 273 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5465 5482 5635 5820 6265 325 Size: 1255 3885 17 153 185 445 1498 Fragments arranged by size: 3885 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2679 3276 5684 Size: 274 80 33 51 1443 702 597 2408 Cuts at: 5684 7396 96 Size: 1712 138 Fragments arranged by size: 2408 1712 1443 702 597 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2762 2794 7398 7430 2762 Size: 32 4604 32 2770 Fragments arranged by size: 4604 2770 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3983 1316 Size: 2667 4771 XbaI T'CTAG_A Cuts at: 4774 4774 Size: 7438 XhoI C'TCGA_G Cuts at: 1943 3069 1943 Size: 1126 6312 XmaI C'CCGG_G Cuts at: 1870 2887 3720 4763 1870 Size: 1017 833 1043 4545 Fragments arranged by size: 4545 1043 1017 833 XmnI GAAnn'nnTTC Cuts at: 3905 5567 3905 Size: 1662 5776 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5246 276 Size: 53 83 186 936 3712 2468 Fragments arranged by size: 3712 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PsrI RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI XcmI