(Linear) (Six-base) MAPSORT of: KIEE6734.seq Check: 6681 from: 1 to: 2733 KIEE6734.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 519 2733 Size: 519 2214 AccI GT'mk_AC Cuts at: 0 77 1982 2733 Size: 77 1905 751 Fragments arranged by size: 1905 751 77 Acc65I G'GTAC_C Cuts at: 0 785 2733 Size: 785 1948 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 228 680 1198 1481 2070 2569 2733 Size: 228 452 518 283 589 499 164 Fragments arranged by size: 589 518 499 452 283 228 164 AfeI AGC'GCT Cuts at: 0 1361 2733 Size: 1361 1372 AflIII A'CryG_T Cuts at: 0 1289 1502 2733 Size: 1289 213 1231 Fragments arranged by size: 1289 1231 213 AhdI GACnn_n'nnGTC Cuts at: 0 2266 2733 Size: 2266 467 AleI CACnn'nnGTG Cuts at: 0 884 2733 Size: 884 1849 AlwNI CAG_nnn'CTG Cuts at: 0 865 2212 2527 2654 2673 2733 Size: 865 1347 315 127 19 60 Fragments arranged by size: 1347 865 315 127 60 19 ApaI G_GGCC'C Cuts at: 0 810 1251 1396 1654 2056 2733 Size: 810 441 145 258 402 677 Fragments arranged by size: 810 677 441 402 258 145 ApoI r'AATT_y Cuts at: 0 172 1132 2733 Size: 172 960 1601 Fragments arranged by size: 1601 960 172 AvaI C'yCGr_G Cuts at: 0 701 882 1064 1437 1715 2733 Size: 701 181 182 373 278 1018 Fragments arranged by size: 1018 701 373 278 182 181 BanI G'GyrC_C Cuts at: 0 57 785 886 987 2733 Size: 57 728 101 101 1746 Fragments arranged by size: 1746 728 101 101 57 BanII G_rGCy'C Cuts at: 0 810 845 1251 1396 1654 1739 2056 2184 Size: 810 35 406 145 258 85 317 128 Cuts at: 2184 2685 2733 Size: 501 48 Fragments arranged by size: 810 501 406 317 258 145 128 85 48 35 BbsI GAAGACnn'nnnn_ Cuts at: 0 1285 1979 2733 Size: 1285 694 754 Fragments arranged by size: 1285 754 694 BbvCI CC'TCA_GC Cuts at: 0 517 2733 Size: 517 2216 BglI GCCn_nnn'nGGC Cuts at: 0 9 620 2733 Size: 9 611 2113 Fragments arranged by size: 2113 611 9 BglII A'GATC_T Cuts at: 0 1529 2733 Size: 1529 1204 BlpI GC'TnA_GC Cuts at: 0 949 2733 Size: 949 1784 Bme1580I G_kGCm'C Cuts at: 0 62 810 891 992 1251 1396 1654 2056 Size: 62 748 81 101 259 145 258 402 Cuts at: 2056 2733 Size: 677 Fragments arranged by size: 748 677 402 259 258 145 101 81 62 BmgBI CAC'GTC Cuts at: 0 42 267 2079 2733 Size: 42 225 1812 654 Fragments arranged by size: 1812 654 225 42 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1055 1548 2733 Size: 1055 493 1185 Fragments arranged by size: 1185 1055 493 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 136 481 745 1384 1630 1694 1807 2733 Size: 136 345 264 639 246 64 113 926 Fragments arranged by size: 926 639 345 264 246 136 113 64 Bpu10I CC'TnA_GC Cuts at: 0 517 2008 2674 2733 Size: 517 1491 666 59 Fragments arranged by size: 1491 666 517 59 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1224 2733 Size: 1224 1509 BsaI GGTCTCn'nnnn_ Cuts at: 0 1026 1290 2158 2253 2733 Size: 1026 264 868 95 480 Fragments arranged by size: 1026 868 480 264 95 BsaAI yAC'GTr Cuts at: 0 95 2733 Size: 95 2638 BsaHI Gr'CG_yC Cuts at: 0 1001 2733 Size: 1001 1732 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1099 1129 2733 Size: 1099 30 1604 Fragments arranged by size: 1604 1099 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 415 601 682 1031 1540 1564 1885 2383 Size: 415 186 81 349 509 24 321 498 Cuts at: 2383 2733 Size: 350 Fragments arranged by size: 509 498 415 350 349 321 186 81 24 BseYI C'CCAG_C Cuts at: 0 596 803 1334 1344 1558 2252 2733 Size: 596 207 531 10 214 694 481 Fragments arranged by size: 694 596 531 481 214 207 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 655 2733 Size: 64 591 2078 Fragments arranged by size: 2078 591 64 BsiHKAI G_wGCw'C Cuts at: 0 407 2391 2733 Size: 407 1984 342 Fragments arranged by size: 1984 407 342 Bsp1286I G_dGCh'C Cuts at: 0 62 407 810 845 891 992 1251 1396 Size: 62 345 403 35 46 101 259 145 Cuts at: 1396 1654 1739 2056 2184 2391 2685 2733 Size: 258 85 317 128 207 294 48 Fragments arranged by size: 403 345 317 294 259 258 207 145 128 101 85 62 48 46 35 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 519 1085 1871 2733 Size: 519 566 786 862 Fragments arranged by size: 862 786 566 519 BsrBI CCG'CTC Cuts at: 0 1614 1620 1626 2322 2678 2733 Size: 1614 6 6 696 356 55 Fragments arranged by size: 1614 696 356 55 6 6 BsrFI r'CCGG_y Cuts at: 0 125 2485 2733 Size: 125 2360 248 Fragments arranged by size: 2360 248 125 BssSI C'ACGA_G Cuts at: 0 1324 2733 Size: 1324 1409 BstEII G'GTnAC_C Cuts at: 0 430 1329 2733 Size: 430 899 1404 Fragments arranged by size: 1404 899 430 BstXI CCAn_nnnn'nTGG Cuts at: 0 459 1308 2433 2733 Size: 459 849 1125 300 Fragments arranged by size: 1125 849 459 300 BstYI r'GATC_y Cuts at: 0 1529 2208 2733 Size: 1529 679 525 Fragments arranged by size: 1529 679 525 Bsu36I CC'TnA_GG Cuts at: 0 377 1483 1919 1964 1996 2733 Size: 377 1106 436 45 32 737 Fragments arranged by size: 1106 737 436 377 45 32 BtgI C'CryG_G Cuts at: 0 7 179 452 1388 1447 1570 2476 2570 Size: 7 172 273 936 59 123 906 94 Cuts at: 2570 2733 Size: 163 Fragments arranged by size: 936 906 273 172 163 123 94 59 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1110 2733 Size: 1110 1623 BtsI GCAGTG_nn' Cuts at: 0 578 2655 2733 Size: 578 2077 78 Fragments arranged by size: 2077 578 78 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1245 1280 2733 Size: 1245 35 1453 Fragments arranged by size: 1453 1245 35 DraIII CAC_nnn'GTG Cuts at: 0 1329 2347 2733 Size: 1329 1018 386 Fragments arranged by size: 1329 1018 386 DrdI GACnn_nn'nnGTC Cuts at: 0 271 454 2733 Size: 271 183 2279 Fragments arranged by size: 2279 271 183 EaeI y'GGCC_r Cuts at: 0 1253 1794 1808 2118 2567 2573 2733 Size: 1253 541 14 310 449 6 160 Fragments arranged by size: 1253 541 449 310 160 14 6 EarI CTCTTCn'nnn_ Cuts at: 0 247 659 1106 2310 2733 Size: 247 412 447 1204 423 Fragments arranged by size: 1204 447 423 412 247 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 136 228 481 680 745 1198 1384 1481 Size: 136 92 253 199 65 453 186 97 Cuts at: 1481 1630 1694 1807 2070 2569 2733 Size: 149 64 113 263 499 164 Fragments arranged by size: 499 453 263 253 199 186 164 149 136 113 97 92 65 64 EcoNI CCTnn'n_nnAGG Cuts at: 0 1486 1867 2733 Size: 1486 381 866 Fragments arranged by size: 1486 866 381 EcoO109I rG'GnC_Cy Cuts at: 0 198 309 381 600 807 862 1248 1393 Size: 198 111 72 219 207 55 386 145 Cuts at: 1393 1650 1651 2052 2053 2113 2670 2733 Size: 257 1 401 1 60 557 63 Fragments arranged by size: 557 401 386 257 219 207 198 145 111 72 63 60 55 1 1 EcoRI G'AATT_C Cuts at: 0 1132 2733 Size: 1132 1601 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 258 2733 Size: 258 2475 HaeII r_GCGC'y Cuts at: 0 132 1363 2733 Size: 132 1231 1370 Fragments arranged by size: 1370 1231 132 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 424 456 1047 1079 2193 2200 2225 2232 Size: 424 32 591 32 1114 7 25 7 Cuts at: 2232 2252 2284 2733 Size: 20 32 449 Fragments arranged by size: 1114 591 449 424 32 32 32 25 20 7 7 HincII GTy'rAC Cuts at: 0 1983 2733 Size: 1983 750 HindIII A'AGCT_T Cuts at: 0 2094 2733 Size: 2094 639 KpnI G_GTAC'C Cuts at: 0 789 2733 Size: 789 1944 MfeI C'AATT_G Cuts at: 0 290 2733 Size: 290 2443 MluI A'CGCG_T Cuts at: 0 1289 2733 Size: 1289 1444 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 146 1574 2021 2450 2733 Size: 146 1428 447 429 283 Fragments arranged by size: 1428 447 429 283 146 MscI TGG'CCA Cuts at: 0 1796 1810 2733 Size: 1796 14 923 Fragments arranged by size: 1796 923 14 MslI CAynn'nnrTG Cuts at: 0 884 2733 Size: 884 1849 MspA1I CmG'CkG Cuts at: 0 9 104 210 321 1572 1767 2518 2733 Size: 9 95 106 111 1251 195 751 215 Fragments arranged by size: 1251 751 215 195 111 106 95 9 NcoI C'CATG_G Cuts at: 0 179 452 1447 2476 2733 Size: 179 273 995 1029 257 Fragments arranged by size: 1029 995 273 257 179 NspI r_CATG'y Cuts at: 0 1506 2733 Size: 1506 1227 PciI A'CATG_T Cuts at: 0 1502 2733 Size: 1502 1231 PflMI CCAn_nnn'nTGG Cuts at: 0 865 2079 2733 Size: 865 1214 654 Fragments arranged by size: 1214 865 654 PfoI T'CCnGG_A Cuts at: 0 260 458 2609 2633 2733 Size: 260 198 2151 24 100 Fragments arranged by size: 2151 260 198 100 24 PmlI CAC'GTG Cuts at: 0 95 2733 Size: 95 2638 PpuMI rG'GwC_Cy Cuts at: 0 198 381 600 2670 2733 Size: 198 183 219 2070 63 Fragments arranged by size: 2070 219 198 183 63 PspOMI G'GGCC_C Cuts at: 0 806 1247 1392 1650 2052 2733 Size: 806 441 145 258 402 681 Fragments arranged by size: 806 681 441 402 258 145 PspXI vC'TCGA_Gb Cuts at: 0 1064 2733 Size: 1064 1669 PstI C_TGCA'G Cuts at: 0 359 656 2179 2523 2733 Size: 359 297 1523 344 210 Fragments arranged by size: 1523 359 344 297 210 PvuII CAG'CTG Cuts at: 0 321 1767 2518 2733 Size: 321 1446 751 215 Fragments arranged by size: 1446 751 321 215 SacII CC_GC'GG Cuts at: 0 10 1573 2733 Size: 10 1563 1160 Fragments arranged by size: 1563 1160 10 SalI G'TCGA_C Cuts at: 0 1981 2733 Size: 1981 752 SanDI GG'GwC_CC Cuts at: 0 600 2733 Size: 600 2133 SfcI C'TryA_G Cuts at: 0 132 355 652 2175 2519 2733 Size: 132 223 297 1523 344 214 Fragments arranged by size: 1523 344 297 223 214 132 SmaI CCC'GGG Cuts at: 0 884 1717 2733 Size: 884 833 1016 Fragments arranged by size: 1016 884 833 SmlI C'TyrA_G Cuts at: 0 1064 1203 2733 Size: 1064 139 1530 Fragments arranged by size: 1530 1064 139 StuI AGG'CCT Cuts at: 0 1070 1175 2013 2733 Size: 1070 105 838 720 Fragments arranged by size: 1070 838 720 105 StyI C'CwwG_G Cuts at: 0 179 452 1447 1811 2476 2733 Size: 179 273 995 364 665 257 Fragments arranged by size: 995 665 364 273 257 179 TatI w'GTAC_w Cuts at: 0 674 1271 2733 Size: 674 597 1462 Fragments arranged by size: 1462 674 597 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 757 789 2733 Size: 757 32 1944 Fragments arranged by size: 1944 757 32 Tth111I GACn'n_nGTC Cuts at: 0 1978 2733 Size: 1978 755 XhoI C'TCGA_G Cuts at: 0 1064 2733 Size: 1064 1669 XmaI C'CCGG_G Cuts at: 0 882 1715 2733 Size: 882 833 1018 Fragments arranged by size: 1018 882 833 XmnI GAAnn'nnTTC Cuts at: 0 1900 2733 Size: 1900 833 Enzymes that do cut: AarI AccI Acc65I AcuI AfeI AflIII AhdI AleI AlwNI ApaI ApoI AvaI BanI BanII BbsI BbvCI BglI BglII BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NspI PciI PflMI PfoI PmlI PpuMI PspOMI PspXI PstI PvuII SacII SalI SanDI SfcI SmaI SmlI StuI StyI TatI TstI Tth111I XhoI XmaI XmnI Enzymes that do not cut: AatII AclI AflII AgeI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI BmrI BmtI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BstAPI BstBI BstZ17I ClaI DraI EagI EciI EcoICRI EcoRV FseI FspI FspAI HpaI KasI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PpiI PshAI PsiI PsrI PvuI RsrII SacI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TaqII XbaI XcmI ZraI