################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## Acc65I AclI AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AvrII BbeI BbrPI BbsI BbvCI BcgI BciVI BfaI BglI BglII BlnI BpuAI BsaI BseRI BsiEI BsiWI BspDI BsrDI BsrFI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FseI HindIII HpaI KasI KpnI KspI MaeI MfeI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SspI SwaI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AatII 211 GACGTC GACGT^C AccI 199 GTCTAC GT^[AC][GT]AC AccIII 1391 TCCGGA T^CCGGA AcsI 980 GAATTC [GA]^AATT[CT] AflII 121 CTTAAG C^TTAAG AhdI 325 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 993 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 268 GGGCCC GGGCC^C ApoI 980 GAATTC [GA]^AATT[CT] AspEI 325 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 628 GAGCTC G[AT]GC[AT]^C BamHI 689 GGATCC G^GATCC BanI 93 GGTGCC G^G[CT][GA]CC BclI 758 TGATCA T^GATCA BfrI 121 CTTAAG C^TTAAG BsaAI 274 TACGTG [CT]AC^GT[GA] BsaBI 174 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1391 TCCGGA [AT]^CCGG[AT] BseAI 1391 TCCGGA T^CCGGA BsgI 1081 CTGCAC GTGCAG(16/14) BsiHKAI 628 GAGCTC G[AT]GC[AT]^C BsmBI 1525 CGTCTC CGTCTC(1/5) BspEI 1391 TCCGGA T^CCGGA BspLU11I 161 ACATGT A^CATGT BssHII 19 GCGCGC G^CGCGC BssSI 697 CTCGTG CACGAG(-5/-1) BstXI 410 CCAACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 694 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 325 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 980 GAATTC G^AATTC FbaI 758 TGATCA T^GATCA HincII 727 GTTGAC GT[CT]^[GA]AC HindII 727 GTTGAC GT[CT]^[GA]AC MamI 174 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1391 TCCGGA T^CCGGA MseI 122 TTAA T^TAA NcoI 1262 CCATGG C^CATGG PaeR7I 340 CTCGAG C^TCGAG PflMI 693 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 628 GAGCTC GAGCT^C SapI 651 GCTCTTC GCTCTTC(1/4) SbfI 998 CCTGCAGG CCTGCA^GG ScaI 578 AGTACT AGT^ACT SmaI 1424 CCCGGG CCC^GGG SphI 1585 GCATGC GCATG^C Sse8387I 998 CCTGCAGG CCTGCA^GG Tru9I 122 TTAA T^TAA Tsp509I 981 AATT ^AATT Van91I 693 CCACCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 340 CTCGAG C^TCGAG XmaCI 1424 CCCGGG C^CCGGG XmaI 1424 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcyI 43 GGCGTC G[GA]^CG[CT]C 211 GACGTC AflIII 161 ACATGT A^C[GA][CT]GT 275 ACGTGT AvaI 340 CTCGAG C^[CT]CG[GA]G 1424 CCCGGG AviII 131 TGCGCA TGC^GCA 1562 TGCGCA BalI 1410 TGGCCA TGG^CCA 1483 TGGCCA BsaHI 43 GGCGTC G[GA]^CG[CT]C 211 GACGTC BsmAI 1273 GAGAC GTCTC(1/5) 1526 GTCTC BsmI 484 GCATTC GAATGC(1/-1) 738 GCATTC BsoBI 340 CTCGAG C^[CT]CG[GA]G 1424 CCCGGG Bsp1407I 278 TGTACA T^GTACA 857 TGTACA BsrBI 1254 GAGCGG CCGCTC(-3/-3) 1542 GAGCGG BsrGI 278 TGTACA T^GTACA 857 TGTACA DrdI 394 GACTACATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 798 GACTCCTGAGTC DsaI 378 CCACGG C^C[GA][CT]GG 1262 CCATGG EarI 652 CTCTTC CTCTTC(1/4) 1277 CTCTTC FspI 131 TGCGCA TGC^GCA 1562 TGCGCA Hin1I 43 GGCGTC G[GA]^CG[CT]C 211 GACGTC Ksp632I 652 CTCTTC CTCTTC(1/4) 1277 CTCTTC MluNI 1410 TGGCCA TGG^CCA 1483 TGGCCA MscI 1410 TGGCCA TGG^CCA 1483 TGGCCA MslI 81 CATGATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 399 CATCGTCATG PstI 999 CTGCAG CTGCA^G 1162 CTGCAG PvuII 1165 CAGCTG CAG^CTG 1297 CAGCTG SmlI 121 CTTAAG C^T[CT][GA]AG 340 CTCGAG SspBI 278 TGTACA T^GTACA 857 TGTACA StuI 833 AGGCCT AGG^CCT 1620 AGGCCT TaqI 137 TCGA T^CGA 341 TCGA TfiI 564 GAATC G^A[AT]TC 1136 GAATC TthHB8I 137 TCGA T^CGA 341 TCGA XcmI 1398 CCAAGTAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1486 CCAACCGCAGCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AvaII BanII BbvI BcnI BlpI BmyI BpmI Bpu1102I BsaJI BsiYI BslI BsmFI Bsp1286I BspHI BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I TspRI XhoII