(Linear) (Six-base) MAPSORT of: KIEE4518.seq Check: 214 from: 1 to: 1656 KIEE4518.orf With 182 enzymes: SgfI * March 22, 2012 18:15 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 243 1006 1656 Size: 243 763 650 Fragments arranged by size: 763 650 243 AatII G_ACGT'C Cuts at: 0 215 1656 Size: 215 1441 AccI GT'mk_AC Cuts at: 0 200 1656 Size: 200 1456 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 256 484 1656 Size: 256 228 1172 Fragments arranged by size: 1172 256 228 AflII C'TTAA_G Cuts at: 0 121 1656 Size: 121 1535 AflIII A'CryG_T Cuts at: 0 161 275 1656 Size: 161 114 1381 Fragments arranged by size: 1381 161 114 AhdI GACnn_n'nnGTC Cuts at: 0 330 1656 Size: 330 1326 AlwNI CAG_nnn'CTG Cuts at: 0 998 1656 Size: 998 658 ApaI G_GGCC'C Cuts at: 0 272 1656 Size: 272 1384 ApoI r'AATT_y Cuts at: 0 980 1656 Size: 980 676 AvaI C'yCGr_G Cuts at: 0 340 1424 1656 Size: 340 1084 232 Fragments arranged by size: 1084 340 232 BamHI G'GATC_C Cuts at: 0 689 1656 Size: 689 967 BanI G'GyrC_C Cuts at: 0 93 1656 Size: 93 1563 BanII G_rGCy'C Cuts at: 0 272 632 653 1504 1656 Size: 272 360 21 851 152 Fragments arranged by size: 851 360 272 152 21 BclI T'GATC_A Cuts at: 0 758 1656 Size: 758 898 BlpI GC'TnA_GC Cuts at: 0 626 718 1168 1656 Size: 626 92 450 488 Fragments arranged by size: 626 488 450 92 Bme1580I G_kGCm'C Cuts at: 0 272 1014 1648 1656 Size: 272 742 634 8 Fragments arranged by size: 742 634 272 8 BmrI ACTGGGnnnn_n' Cuts at: 0 137 1217 1326 1656 Size: 137 1080 109 330 Fragments arranged by size: 1080 330 137 109 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1119 1523 1656 Size: 1119 404 133 Fragments arranged by size: 1119 404 133 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 835 991 1493 1518 1656 Size: 835 156 502 25 138 Fragments arranged by size: 835 502 156 138 25 BsaAI yAC'GTr Cuts at: 0 276 1656 Size: 276 1380 BsaBI GATnn'nnATC Cuts at: 0 178 1656 Size: 178 1478 BsaHI Gr'CG_yC Cuts at: 0 44 212 1656 Size: 44 168 1444 Fragments arranged by size: 1444 168 44 BsaWI w'CCGG_w Cuts at: 0 1391 1656 Size: 1391 265 BseYI C'CCAG_C Cuts at: 0 23 39 69 223 1350 1451 1553 1656 Size: 23 16 30 154 1127 101 102 103 Fragments arranged by size: 1127 154 103 102 101 30 23 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1066 1656 Size: 1066 590 BsiHKAI G_wGCw'C Cuts at: 0 632 1656 Size: 632 1024 BsmI GAATG_Cn' Cuts at: 0 484 738 1656 Size: 484 254 918 Fragments arranged by size: 918 484 254 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1531 1656 Size: 1531 125 Bsp1286I G_dGCh'C Cuts at: 0 272 632 653 1014 1504 1648 1656 Size: 272 360 21 361 490 144 8 Fragments arranged by size: 490 361 360 272 144 21 8 BspEI T'CCGG_A Cuts at: 0 1391 1656 Size: 1391 265 BspHI T'CATG_A Cuts at: 0 80 215 473 512 794 1656 Size: 80 135 258 39 282 862 Fragments arranged by size: 862 282 258 135 80 39 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 105 243 992 1006 1656 Size: 105 138 749 14 650 Fragments arranged by size: 749 650 138 105 14 BsrBI CCG'CTC Cuts at: 0 1256 1544 1656 Size: 1256 288 112 Fragments arranged by size: 1256 288 112 BsrGI T'GTAC_A Cuts at: 0 278 857 1656 Size: 278 579 799 Fragments arranged by size: 799 579 278 BssHII G'CGCG_C Cuts at: 0 19 1656 Size: 19 1637 BssSI C'ACGA_G Cuts at: 0 697 1656 Size: 697 959 BstXI CCAn_nnnn'nTGG Cuts at: 0 417 1656 Size: 417 1239 BstYI r'GATC_y Cuts at: 0 173 230 689 1656 Size: 173 57 459 967 Fragments arranged by size: 967 459 173 57 BtgI C'CryG_G Cuts at: 0 378 1262 1656 Size: 378 884 394 Fragments arranged by size: 884 394 378 BtsI GCAGTG_nn' Cuts at: 0 1548 1656 Size: 1548 108 DraIII CAC_nnn'GTG Cuts at: 0 699 1656 Size: 699 957 DrdI GACnn_nn'nnGTC Cuts at: 0 400 804 1656 Size: 400 404 852 Fragments arranged by size: 852 404 400 EaeI y'GGCC_r Cuts at: 0 784 1410 1483 1656 Size: 784 626 73 173 Fragments arranged by size: 784 626 173 73 EarI CTCTTCn'nnn_ Cuts at: 0 658 1283 1656 Size: 658 625 373 Fragments arranged by size: 658 625 373 EcoICRI GAG'CTC Cuts at: 0 630 1656 Size: 630 1026 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 256 484 835 991 1493 1518 1656 Size: 256 228 351 156 502 25 138 Fragments arranged by size: 502 351 256 228 156 138 25 EcoO109I rG'GnC_Cy Cuts at: 0 117 269 552 1463 1627 1656 Size: 117 152 283 911 164 29 Fragments arranged by size: 911 283 164 152 117 29 EcoRI G'AATT_C Cuts at: 0 980 1656 Size: 980 676 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 747 1656 Size: 747 909 FspI TGC'GCA Cuts at: 0 133 1564 1656 Size: 133 1431 92 Fragments arranged by size: 1431 133 92 FspAI rTGC'GCAy Cuts at: 0 133 1656 Size: 133 1523 HaeII r_GCGC'y Cuts at: 0 40 77 1351 1656 Size: 40 37 1274 305 Fragments arranged by size: 1274 305 40 37 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 316 348 1656 Size: 316 32 1308 Fragments arranged by size: 1308 316 32 HincII GTy'rAC Cuts at: 0 729 1656 Size: 729 927 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 665 1656 Size: 665 991 MscI TGG'CCA Cuts at: 0 1412 1485 1656 Size: 1412 73 171 Fragments arranged by size: 1412 171 73 MslI CAynn'nnrTG Cuts at: 0 85 403 1656 Size: 85 318 1253 Fragments arranged by size: 1253 318 85 MspA1I CmG'CkG Cuts at: 0 1167 1236 1299 1656 Size: 1167 69 63 357 Fragments arranged by size: 1167 357 69 63 NcoI C'CATG_G Cuts at: 0 1262 1656 Size: 1262 394 NspI r_CATG'y Cuts at: 0 165 594 1589 1656 Size: 165 429 995 67 Fragments arranged by size: 995 429 165 67 PciI A'CATG_T Cuts at: 0 161 1656 Size: 161 1495 PflMI CCAn_nnn'nTGG Cuts at: 0 699 1656 Size: 699 957 PfoI T'CCnGG_A Cuts at: 0 1112 1656 Size: 1112 544 PpuMI rG'GwC_Cy Cuts at: 0 117 552 1627 1656 Size: 117 435 1075 29 Fragments arranged by size: 1075 435 117 29 PspOMI G'GGCC_C Cuts at: 0 268 1656 Size: 268 1388 PspXI vC'TCGA_Gb Cuts at: 0 340 1656 Size: 340 1316 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 271 303 1656 Size: 271 32 1353 Fragments arranged by size: 1353 271 32 PstI C_TGCA'G Cuts at: 0 1003 1166 1656 Size: 1003 163 490 Fragments arranged by size: 1003 490 163 PvuII CAG'CTG Cuts at: 0 1167 1299 1656 Size: 1167 132 357 Fragments arranged by size: 1167 357 132 SacI G_AGCT'C Cuts at: 0 632 1656 Size: 632 1024 SanDI GG'GwC_CC Cuts at: 0 1627 1656 Size: 1627 29 SapI GCTCTTCn'nnn_ Cuts at: 0 658 1656 Size: 658 998 SbfI CC_TGCA'GG Cuts at: 0 1003 1656 Size: 1003 653 ScaI AGT'ACT Cuts at: 0 580 1656 Size: 580 1076 SfcI C'TryA_G Cuts at: 0 822 999 1162 1656 Size: 822 177 163 494 Fragments arranged by size: 822 494 177 163 SmaI CCC'GGG Cuts at: 0 1426 1656 Size: 1426 230 SmlI C'TyrA_G Cuts at: 0 121 340 1656 Size: 121 219 1316 Fragments arranged by size: 1316 219 121 SphI G_CATG'C Cuts at: 0 1589 1656 Size: 1589 67 SrfI GCCC'GGGC Cuts at: 0 1426 1656 Size: 1426 230 StuI AGG'CCT Cuts at: 0 835 1622 1656 Size: 835 787 34 Fragments arranged by size: 835 787 34 StyI C'CwwG_G Cuts at: 0 479 1262 1313 1635 1656 Size: 479 783 51 322 21 Fragments arranged by size: 783 479 322 51 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 415 517 960 1656 Size: 415 102 443 696 Fragments arranged by size: 696 443 415 102 TatI w'GTAC_w Cuts at: 0 278 578 857 1656 Size: 278 300 279 799 Fragments arranged by size: 799 300 279 278 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1405 1493 1656 Size: 1405 88 163 Fragments arranged by size: 1405 163 88 XhoI C'TCGA_G Cuts at: 0 340 1656 Size: 340 1316 XmaI C'CCGG_G Cuts at: 0 1424 1656 Size: 1424 232 ZraI GAC'GTC Cuts at: 0 213 1656 Size: 213 1443 Enzymes that do cut: AarI AatII AccI AcuI AflII AflIII AhdI AlwNI ApaI ApoI AvaI BamHI BanI BanII BclI BlpI Bme1580I BmrI BplI BpmI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI BstXI BstYI BtgI BtsI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII MmeI MscI MslI MspA1I NcoI NspI PciI PflMI PfoI PpuMI PspOMI PspXI PsrI PstI PvuII SacI SanDI SapI SbfI ScaI SfcI SmaI SmlI SphI SrfI StuI StyI TaqII TatI XcmI XhoI XmaI ZraI Enzymes that do not cut: Acc65I AclI AfeI AgeI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BglII BmgBI BmtI Bpu10I BpuEI BsaI BsaXI BseRI BsiEI BsiWI BsrDI BsrFI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI EagI EciI EcoNI EcoRV FseI HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PmlI PpiI PshAI PsiI PvuI RsrII SacII SalI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SspI SwaI TstI Tth111I XbaI XmnI