(Circular) (Six-base) MAPSORT of: pFN21AB8470.seq Check: 7230 from: 1 to: 7171 pFN21AB8470 7171 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2657 2657 Size: 7171 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3270 4212 4981 278 Size: 53 83 186 936 1734 942 769 2468 Fragments arranged by size: 2468 1734 942 936 769 186 83 53 AccI GT'mk_AC Cuts at: 1937 3999 4452 4514 1937 Size: 2062 453 62 4594 Fragments arranged by size: 4594 2062 453 62 Acc65I G'GTAC_C Cuts at: 4266 4492 4266 Size: 226 6945 AclI AA'CG_TT Cuts at: 1563 5298 5671 1563 Size: 3735 373 3063 Fragments arranged by size: 3735 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2933 3746 4409 5234 6618 6886 1632 Size: 1301 813 663 825 1384 268 1917 Fragments arranged by size: 1917 1384 1301 825 813 663 268 AfeI AGC'GCT Cuts at: 2946 2946 Size: 7171 AflII C'TTAA_G Cuts at: 820 1017 2752 3365 820 Size: 197 1735 613 4626 Fragments arranged by size: 4626 1735 613 197 AflIII A'CryG_T Cuts at: 1203 3007 4104 6070 7047 1203 Size: 1804 1097 1966 977 1327 Fragments arranged by size: 1966 1804 1327 1097 977 AgeI A'CCGG_T Cuts at: 3281 6002 3281 Size: 2721 4450 AhdI GACnn_n'nnGTC Cuts at: 5900 5900 Size: 7171 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7171 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7171 AlwNI CAG_nnn'CTG Cuts at: 3503 3964 4047 4914 6486 3503 Size: 461 83 867 1572 4188 Fragments arranged by size: 4188 1572 867 461 83 ApaLI G'TGCA_C Cuts at: 4022 5228 6384 4022 Size: 1206 1156 4809 Fragments arranged by size: 4809 1206 1156 ApoI r'AATT_y Cuts at: 1493 2214 2315 4190 4480 4706 4858 6967 1493 Size: 721 101 1875 290 226 152 2109 1697 Fragments arranged by size: 2109 1875 1697 721 290 226 152 101 AseI AT'TA_AT Cuts at: 160 1032 5725 160 Size: 872 4693 1606 Fragments arranged by size: 4693 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7171 AvaI C'yCGr_G Cuts at: 1870 1943 4098 4496 1870 Size: 73 2155 398 4545 Fragments arranged by size: 4545 2155 398 73 BamHI G'GATC_C Cuts at: 4501 4868 4501 Size: 367 6804 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4266 4492 5947 618 Size: 325 217 627 2479 226 1455 1842 Fragments arranged by size: 2479 1842 1455 627 325 226 217 BanII G_rGCy'C Cuts at: 1391 2750 3777 4386 4490 1391 Size: 1359 1027 609 104 4072 Fragments arranged by size: 4072 1359 1027 609 104 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3725 928 Size: 971 1826 4374 Fragments arranged by size: 4374 1826 971 BbvCI CC'TCA_GC Cuts at: 3924 3924 Size: 7171 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5362 5396 1903 Size: 34 3425 34 3678 Fragments arranged by size: 3678 3425 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5063 6273 5063 Size: 1210 5961 BclI T'GATC_A Cuts at: 1548 1548 Size: 7171 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5782 136 Size: 107 122 71 631 605 4110 1525 Fragments arranged by size: 4110 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2793 3702 7166 2793 Size: 909 3464 2798 Fragments arranged by size: 3464 2798 909 BlpI GC'TnA_GC Cuts at: 4588 4588 Size: 7171 Bme1580I G_kGCm'C Cuts at: 1790 3898 4026 4340 5232 6388 1790 Size: 2108 128 314 892 1156 2573 Fragments arranged by size: 2573 2108 1156 892 314 128 BmgBI CAC'GTC Cuts at: 1318 2106 4105 1318 Size: 788 1999 4384 Fragments arranged by size: 4384 1999 788 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3541 4902 5860 Size: 416 53 83 399 24 2124 1361 958 Cuts at: 5860 442 Size: 1753 Fragments arranged by size: 2124 1753 1361 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7171 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3489 3489 Size: 7171 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2101 3026 3773 4152 4234 5831 1239 Size: 862 925 747 379 82 1597 2579 Fragments arranged by size: 2579 1597 925 862 747 379 82 Bpu10I CC'TnA_GC Cuts at: 3690 3924 3690 Size: 234 6937 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3560 5296 6161 6459 3560 Size: 1736 865 298 4272 Fragments arranged by size: 4272 1736 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5834 882 Size: 617 4335 2219 Fragments arranged by size: 4335 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4335 4962 493 Size: 711 3131 627 2702 Fragments arranged by size: 3131 2702 711 627 BsaBI GATnn'nnATC Cuts at: 7165 7165 Size: 7171 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3267 3819 4007 4209 Size: 53 83 186 936 1734 552 188 202 Cuts at: 4209 4978 5360 5995 275 Size: 769 382 635 1451 Fragments arranged by size: 1734 1451 936 769 635 552 382 202 188 186 83 53 BsaWI w'CCGG_w Cuts at: 2674 3281 4447 5604 6002 6276 6423 6834 2674 Size: 607 1166 1157 398 274 147 411 3011 Fragments arranged by size: 3011 1166 1157 607 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6946 Fragments arranged by size: 6946 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7171 BseYI C'CCAG_C Cuts at: 3403 3975 4011 4363 6374 6870 3403 Size: 572 36 352 2011 496 3704 Fragments arranged by size: 3704 2011 572 496 352 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2158 1163 Size: 573 422 6176 Fragments arranged by size: 6176 573 422 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3286 4621 5382 6410 1540 Size: 275 186 1285 1335 761 1028 2301 Fragments arranged by size: 2301 1335 1285 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3777 3801 4026 5232 5317 6388 3777 Size: 24 225 1206 85 1071 4560 Fragments arranged by size: 4560 1206 1071 225 85 24 BsmI GAATG_Cn' Cuts at: 2910 3677 4682 4775 2910 Size: 767 1005 93 5306 Fragments arranged by size: 5306 1005 767 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2750 3777 3801 3898 4026 4340 4386 Size: 399 960 1027 24 97 128 314 46 Cuts at: 4386 4490 5232 5317 6388 1391 Size: 104 742 85 1071 2174 Fragments arranged by size: 2174 1071 1027 960 742 399 314 128 104 97 85 46 24 BspEI T'CCGG_A Cuts at: 6834 6834 Size: 7171 BspHI T'CATG_A Cuts at: 5060 6790 5060 Size: 1730 5441 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2361 2577 2657 4526 844 Size: 1517 216 80 1869 3489 Fragments arranged by size: 3489 1869 1517 216 80 BsrBI CCG'CTC Cuts at: 1394 1823 5058 1394 Size: 429 3235 3507 Fragments arranged by size: 3507 3235 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3427 5666 5840 65 Size: 1183 2179 2239 174 1396 Fragments arranged by size: 2239 2179 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3281 5815 6002 1700 Size: 110 1471 2534 187 2869 Fragments arranged by size: 2869 2534 1471 187 110 BsrGI T'GTAC_A Cuts at: 96 4107 4182 96 Size: 4011 75 3085 Fragments arranged by size: 4011 3085 75 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7171 BssSI C'ACGA_G Cuts at: 5231 6243 5231 Size: 1012 6159 BstAPI GCAn_nnn'nTGC Cuts at: 794 3964 4021 794 Size: 3170 57 3944 Fragments arranged by size: 3944 3170 57 BstBI TT'CG_AA Cuts at: 5975 5975 Size: 7171 BstEII G'GTnAC_C Cuts at: 1184 2702 1184 Size: 1518 5653 BstXI CCAn_nnnn'nTGG Cuts at: 3554 3554 Size: 7171 BstYI r'GATC_y Cuts at: 1972 2130 2194 2793 3525 3702 4501 4868 5253 Size: 158 64 599 732 177 799 367 385 Cuts at: 5253 5270 6722 6808 6820 7166 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 799 732 599 385 367 346 177 158 86 64 17 12 Bsu36I CC'TnA_GG Cuts at: 3890 3890 Size: 7171 BtgI C'CryG_G Cuts at: 513 1065 2037 3031 3939 3987 513 Size: 552 972 994 908 48 3697 Fragments arranged by size: 3697 994 972 908 552 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2206 487 Size: 683 56 980 5452 Fragments arranged by size: 5452 980 683 56 BtsI GCAGTG_nn' Cuts at: 2125 3720 4687 5481 5501 2125 Size: 1595 967 794 20 3795 Fragments arranged by size: 3795 1595 967 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7171 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7136 DraI TTT'AAA Cuts at: 4475 4475 Size: 7171 DraIII CAC_nnn'GTG Cuts at: 4021 4021 Size: 7171 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2392 6178 809 Size: 507 1076 3786 1802 Fragments arranged by size: 3786 1802 1076 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2607 4035 4135 Size: 54 1425 50 275 18 777 1428 100 Cuts at: 4135 4434 4618 5507 7136 8 Size: 299 184 889 1629 43 Fragments arranged by size: 1629 1428 1425 889 777 299 275 184 100 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4618 1537 Size: 275 2806 4090 Fragments arranged by size: 4090 2806 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5101 1353 Size: 3748 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2822 5758 6132 6278 387 Size: 1064 138 1233 2936 374 146 1280 Fragments arranged by size: 2936 1280 1233 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3775 3775 Size: 7171 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2101 2933 3026 3746 3773 4152 4234 Size: 393 469 832 93 720 27 379 82 Cuts at: 4234 4409 5234 5831 6618 6886 1239 Size: 175 825 597 787 268 1524 Fragments arranged by size: 1524 832 825 787 720 597 469 393 379 268 175 93 82 27 EcoNI CCTnn'n_nnAGG Cuts at: 2017 2017 Size: 7171 EcoO109I rG'GnC_Cy Cuts at: 3874 3874 Size: 7171 EcoRI G'AATT_C Cuts at: 4480 4858 4480 Size: 378 6793 FspI TGC'GCA Cuts at: 5677 5677 Size: 7171 HaeII r_GCGC'y Cuts at: 1678 2948 6318 1678 Size: 1270 3370 2531 Fragments arranged by size: 3370 2531 1270 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2470 2486 2502 2518 3115 Size: 32 135 32 898 16 16 16 597 Cuts at: 3115 3147 3416 3448 5812 5844 5886 5918 1373 Size: 32 269 32 2364 32 42 32 2626 Fragments arranged by size: 2626 2364 898 597 269 135 42 32 32 32 32 32 32 16 16 16 HincII GTy'rAC Cuts at: 669 1657 1938 2842 4515 4761 669 Size: 988 281 904 1673 246 3079 Fragments arranged by size: 3079 1673 988 904 281 246 HindIII A'AGCT_T Cuts at: 748 2268 748 Size: 1520 5651 HpaI GTT'AAC Cuts at: 4761 4761 Size: 7171 KpnI G_GTAC'C Cuts at: 4270 4496 4270 Size: 226 6945 MfeI C'AATT_G Cuts at: 2295 2581 4770 2295 Size: 286 2189 4696 Fragments arranged by size: 4696 2189 286 MluI A'CGCG_T Cuts at: 7047 7047 Size: 7171 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2912 6285 6469 1305 Size: 1607 3373 184 2007 Fragments arranged by size: 3373 2007 1607 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4037 4137 4436 10 Size: 54 1425 343 2205 100 299 2745 Fragments arranged by size: 2745 2205 1425 343 299 100 54 MslI CAynn'nnrTG Cuts at: 518 1166 2455 2724 3062 5129 5488 5647 518 Size: 648 1289 269 338 2067 359 159 2042 Fragments arranged by size: 2067 2042 1289 648 359 338 269 159 MspA1I CmG'CkG Cuts at: 1606 3046 3989 4273 4587 5264 6412 6657 1606 Size: 1440 943 284 314 677 1148 245 2120 Fragments arranged by size: 2120 1440 1148 943 677 314 284 245 NcoI C'CATG_G Cuts at: 513 1065 2037 513 Size: 552 972 5647 Fragments arranged by size: 5647 972 552 NdeI CA'TA_TG Cuts at: 387 2721 387 Size: 2334 4837 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7171 NotI GC'GGCC_GC Cuts at: 4618 4618 Size: 7171 NspI r_CATG'y Cuts at: 1132 1229 2460 3011 4529 6074 1132 Size: 97 1231 551 1518 1545 2229 Fragments arranged by size: 2229 1545 1518 1231 551 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7171 PciI A'CATG_T Cuts at: 3007 6070 3007 Size: 3063 4108 PflMI CCAn_nnn'nTGG Cuts at: 1108 4158 1108 Size: 3050 4121 PfoI T'CCnGG_A Cuts at: 2012 2012 Size: 7171 PmeI GTTT'AAAC Cuts at: 4475 4475 Size: 7171 PmlI CAC'GTG Cuts at: 4335 4335 Size: 7171 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5240 5272 6753 6785 5240 Size: 32 1481 32 5626 Fragments arranged by size: 5626 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3874 3874 Size: 7171 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7171 PsiI TTA'TAA Cuts at: 4741 4741 Size: 7171 PstI C_TGCA'G Cuts at: 830 2358 2574 3214 4323 4523 830 Size: 1528 216 640 1109 200 3478 Fragments arranged by size: 3478 1528 1109 640 216 200 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7171 PvuII CAG'CTG Cuts at: 3046 4273 3046 Size: 1227 5944 SacI G_AGCT'C Cuts at: 3777 3777 Size: 7171 SacII CC_GC'GG Cuts at: 3990 3990 Size: 7171 SalI G'TCGA_C Cuts at: 1936 4513 1936 Size: 2577 4594 SbfI CC_TGCA'GG Cuts at: 4523 4523 Size: 7171 ScaI AGT'ACT Cuts at: 3551 5419 7131 3551 Size: 1868 1712 3591 Fragments arranged by size: 3591 1868 1712 SexAI A'CCwGG_T Cuts at: 2706 2706 Size: 7171 SfcI C'TryA_G Cuts at: 826 1045 2354 2570 2656 3210 4123 4319 4519 Size: 219 1309 216 86 554 913 196 200 Cuts at: 4519 5654 6335 6526 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1309 1135 913 681 554 219 216 200 196 191 86 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7171 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7171 SmaI CCC'GGG Cuts at: 1872 4100 4498 1872 Size: 2228 398 4545 Fragments arranged by size: 4545 2228 398 SmlI C'TyrA_G Cuts at: 820 1017 1943 2752 3365 3575 5275 6176 6438 Size: 197 926 809 613 210 1700 901 262 Cuts at: 6438 820 Size: 1553 Fragments arranged by size: 1700 1553 926 901 809 613 262 210 197 SnaBI TAC'GTA Cuts at: 493 4962 493 Size: 4469 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7171 SphI G_CATG'C Cuts at: 1132 4529 1132 Size: 3397 3774 SspI AAT'ATT Cuts at: 5 52 2554 2973 5095 5 Size: 47 2502 419 2122 2081 Fragments arranged by size: 2502 2122 2081 419 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7171 StyI C'CwwG_G Cuts at: 513 1065 2037 4437 4610 513 Size: 552 972 2400 173 3074 Fragments arranged by size: 3074 2400 972 552 173 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3289 5198 5215 5368 5553 5998 325 Size: 1255 1709 1909 17 153 185 445 1498 Fragments arranged by size: 1909 1709 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2526 2996 3549 Size: 274 80 33 51 1443 549 470 553 Cuts at: 3549 3858 4107 4182 5417 7129 96 Size: 309 249 75 1235 1712 138 Fragments arranged by size: 1712 1443 1235 553 549 470 309 274 249 138 80 75 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7131 7163 7131 Size: 32 7139 Tth111I GACn'n_nGTC Cuts at: 1316 3952 1316 Size: 2636 4535 XbaI T'CTAG_A Cuts at: 4507 4507 Size: 7171 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2949 4048 2949 Size: 1099 6072 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7171 XmaI C'CCGG_G Cuts at: 1870 4098 4496 1870 Size: 2228 398 4545 Fragments arranged by size: 4545 2228 398 XmnI GAAnn'nnTTC Cuts at: 2405 5300 2405 Size: 2895 4276 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3268 4210 4979 276 Size: 53 83 186 936 1734 942 769 2468 Fragments arranged by size: 2468 1734 942 936 769 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SanDI SapI SfiI SfoI SrfI SwaI