################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## Acc65I AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaII AviII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BpuAI BsaAI BsaBI BsaI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI BspDI BspHI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI MamI MfeI MluI MunI NarI NdeI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 36 GACGTC GACGT^C AccI 1017 GTCTAC GT^[AC][GT]AC AccII 10 CGCG CG^CG AccIII 743 TCCGGA T^CCGGA AlwNI 583 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 859 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 331 CCCGAG C^[CT]CG[GA]G AvrII 684 CCTAGG C^CTAGG BamHI 355 GGATCC G^GATCC BanI 132 GGCACC G^G[CT][GA]CC BglI 397 GCCAAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 684 CCTAGG C^CTAGG BlpI 979 GCTCAGC GC^T[ACGT]AGC Bpu1102I 979 GCTCAGC GC^T[ACGT]AGC BsaWI 743 TCCGGA [AT]^CCGG[AT] BseAI 743 TCCGGA T^CCGGA BsmFI 219 GGGAC GGGAC(10/14) BsoBI 331 CCCGAG C^[CT]CG[GA]G Bsp1407I 140 TGTACA T^GTACA BspEI 743 TCCGGA T^CCGGA BspMI 534 ACCTGC ACCTGC(4/8) BsrFI 622 GCCGGC [GA]^CCGG[CT] BsrGI 140 TGTACA T^GTACA BstAPI 237 GCACATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 10 CGCG CG^CG BstYI 355 GGATCC [GA]^GATC[CT] CelII 979 GCTCAGC GC^T[ACGT]AGC Cfr10I 622 GCCGGC [GA]^CCGG[CT] DdeI 980 CTCAG C^T[ACGT]AG DrdI 36 GACGTCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FauI 351 GCGGG CCCGC(4/6) HaeII 508 AGCGCC [GA]GCGC^[CT] HgaI 1108 GACGC GACGC(5/10) KspI 9 CCGCGG CCGC^GG MaeII 37 ACGT A^CGT MflI 355 GGATCC [GA]^GATC[CT] MroI 743 TCCGGA T^CCGGA MseI 674 TTAA T^TAA MslI 1080 CATCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 10 CGCG CG^CG NaeI 622 GCCGGC GCC^GGC NcoI 795 CCATGG C^CATGG NgoMIV 622 GCCGGC G^CCGGC NheI 339 GCTAGC G^CTAGC SacII 9 CCGCGG CCGC^GG SapI 265 GAAGAGC GCTCTTC(1/4) SspBI 140 TGTACA T^GTACA TaqI 4 TCGA T^CGA Tru9I 674 TTAA T^TAA Tsp509I 616 AATT ^AATT TthHB8I 4 TCGA T^CGA XcmI 359 CCAAAGGAAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 355 GGATCC [GA]^GATC[CT] XmnI 859 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 36 GACGTC G[GA]^CG[CT]C 1108 GACGCC ApaI 461 GGGCCC GGGCC^C 757 GGGCCC BanII 461 GGGCCC G[GA]GC[CT]^C 757 GGGCCC BcnI 233 CCCGG CC^[GC]GG 464 CCCGG BfaI 340 CTAG C^TAG 685 CTAG BsaHI 36 GACGTC G[GA]^CG[CT]C 1108 GACGCC BsgI 845 GTGCAG GTGCAG(16/14) 1087 GTGCAG BsmAI 206 GAGAC GTCTC(1/5) 411 GTCTC BsrDI 924 CATTGC GCAATG(2/0) 194 GCAATG CfoI 509 GCGC GCG^C 788 GCGC DpnII 356 GATC ^GATC 819 GATC DraII 460 GGGGCCC [GA]G^G[ACGT]CC[CT] 757 GGGCCCT EcoO109I 460 GGGGCCC [GA]G^G[ACGT]CC[CT] 757 GGGCCCT HhaI 509 GCGC GCG^C 788 GCGC Hin1I 36 GACGTC G[GA]^CG[CT]C 1108 GACGCC HinP1I 509 GCGC G^CGC 788 GCGC HphI 487 GGTGA GGTGA(8/7) 293 TCACC MaeI 340 CTAG C^TAG 685 CTAG MboI 356 GATC ^GATC 819 GATC NciI 233 CCCGG CC^[GC]GG 464 CCCGG NdeII 356 GATC ^GATC 819 GATC NlaIII 144 CATG CATG^ 796 CATG PleI 59 GAGTC GAGTC(4/5) 290 GAGTC PvuII 277 CAGCTG CAG^CTG 982 CAGCTG Sau3AI 356 GATC ^GATC 819 GATC SfaNI 81 GATGC GCATC(5/9) 1079 GCATC Tsp45I 226 GTCAC ^GT[GC]AC 292 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AciI AfaI AluI AlwI BalI BbvI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DsaI EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI Ksp632I MaeIII MboII MluNI MnlI MscI MspA1I MspI MvaI MwoI NlaIV RsaI Sau96I ScrFI StyI TfiI TseI TspRI