(Linear) (Six-base) MAPSORT of: KIEE3963.seq Check: 650 from: 1 to: 1404 KIEE3963.orf With 182 enzymes: SgfI * March 22, 2012 16:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 52 1404 Size: 52 1352 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 929 1404 Size: 929 475 AfeI AGC'GCT Cuts at: 0 346 970 1006 1404 Size: 346 624 36 398 Fragments arranged by size: 624 398 346 36 AflIII A'CryG_T Cuts at: 0 152 1404 Size: 152 1252 AlwNI CAG_nnn'CTG Cuts at: 0 585 588 597 951 1404 Size: 585 3 9 354 453 Fragments arranged by size: 585 453 354 9 3 ApaI G_GGCC'C Cuts at: 0 148 1065 1404 Size: 148 917 339 Fragments arranged by size: 917 339 148 AvaI C'yCGr_G Cuts at: 0 139 160 694 825 1288 1302 1404 Size: 139 21 534 131 463 14 102 Fragments arranged by size: 534 463 139 131 102 21 14 AvrII C'CTAG_G Cuts at: 0 651 1404 Size: 651 753 BamHI G'GATC_C Cuts at: 0 72 1133 1404 Size: 72 1061 271 Fragments arranged by size: 1061 271 72 BanI G'GyrC_C Cuts at: 0 236 1404 Size: 236 1168 BanII G_rGCy'C Cuts at: 0 148 683 695 1065 1404 Size: 148 535 12 370 339 Fragments arranged by size: 535 370 339 148 12 BbeI G_GCGC'C Cuts at: 0 240 1404 Size: 240 1164 BbsI GAAGACnn'nnnn_ Cuts at: 0 404 544 917 1134 1404 Size: 404 140 373 217 270 Fragments arranged by size: 404 373 270 217 140 BbvCI CC'TCA_GC Cuts at: 0 805 1404 Size: 805 599 BglI GCCn_nnn'nGGC Cuts at: 0 229 1404 Size: 229 1175 BlpI GC'TnA_GC Cuts at: 0 677 1404 Size: 677 727 Bme1580I G_kGCm'C Cuts at: 0 148 160 277 1065 1404 Size: 148 12 117 788 339 Fragments arranged by size: 788 339 148 117 12 BmgBI CAC'GTC Cuts at: 0 864 1404 Size: 864 540 BmrI ACTGGGnnnn_n' Cuts at: 0 369 1038 1404 Size: 369 669 366 Fragments arranged by size: 669 369 366 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 13 436 448 619 634 1404 Size: 13 423 12 171 15 770 Fragments arranged by size: 770 423 171 15 13 12 Bpu10I CC'TnA_GC Cuts at: 0 805 893 1404 Size: 805 88 511 Fragments arranged by size: 805 511 88 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 388 1404 Size: 388 1016 BsaI GGTCTCn'nnnn_ Cuts at: 0 478 1404 Size: 478 926 BsaBI GATnn'nnATC Cuts at: 0 1357 1404 Size: 1357 47 BsaHI Gr'CG_yC Cuts at: 0 237 746 1404 Size: 237 509 658 Fragments arranged by size: 658 509 237 BsaWI w'CCGG_w Cuts at: 0 75 437 1404 Size: 75 362 967 Fragments arranged by size: 967 362 75 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1380 1404 Size: 1380 24 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 49 586 589 616 619 622 1404 Size: 49 537 3 27 3 3 782 Fragments arranged by size: 782 537 49 27 3 3 3 BseYI C'CCAG_C Cuts at: 0 128 534 993 1058 1404 Size: 128 406 459 65 346 Fragments arranged by size: 459 406 346 128 65 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 226 837 946 1095 1404 Size: 226 611 109 149 309 Fragments arranged by size: 611 309 226 149 109 BsiEI CG_ry'CG Cuts at: 0 379 395 1369 1404 Size: 379 16 974 35 Fragments arranged by size: 974 379 35 16 BsiHKAI G_wGCw'C Cuts at: 0 359 683 695 1404 Size: 359 324 12 709 Fragments arranged by size: 709 359 324 12 BsiWI C'GTAC_G Cuts at: 0 149 1404 Size: 149 1255 Bsp1286I G_dGCh'C Cuts at: 0 148 160 277 359 683 695 1065 1404 Size: 148 12 117 82 324 12 370 339 Fragments arranged by size: 370 339 324 148 117 82 12 12 BspEI T'CCGG_A Cuts at: 0 437 1404 Size: 437 967 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 52 200 496 1404 Size: 52 148 296 908 Fragments arranged by size: 908 296 148 52 BsrFI r'CCGG_y Cuts at: 0 255 1364 1404 Size: 255 1109 40 Fragments arranged by size: 1109 255 40 BstAPI GCAn_nnn'nTGC Cuts at: 0 372 531 585 588 951 1404 Size: 372 159 54 3 363 453 Fragments arranged by size: 453 372 363 159 54 3 BstXI CCAn_nnnn'nTGG Cuts at: 0 1209 1404 Size: 1209 195 BstYI r'GATC_y Cuts at: 0 72 1133 1214 1292 1404 Size: 72 1061 81 78 112 Fragments arranged by size: 1061 112 81 78 72 Bsu36I CC'TnA_GG Cuts at: 0 1014 1404 Size: 1014 390 BtgI C'CryG_G Cuts at: 0 1115 1404 Size: 1115 289 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 669 1404 Size: 669 735 BtsI GCAGTG_nn' Cuts at: 0 841 1270 1404 Size: 841 429 134 Fragments arranged by size: 841 429 134 DraIII CAC_nnn'GTG Cuts at: 0 372 1404 Size: 372 1032 EaeI y'GGCC_r Cuts at: 0 2 93 221 296 376 392 1366 1404 Size: 2 91 128 75 80 16 974 38 Fragments arranged by size: 974 128 91 80 75 38 16 2 EagI C'GGCC_G Cuts at: 0 376 392 1366 1404 Size: 376 16 974 38 Fragments arranged by size: 974 376 38 16 EarI CTCTTCn'nnn_ Cuts at: 0 1327 1404 Size: 1327 77 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 493 933 1404 Size: 493 440 471 Fragments arranged by size: 493 471 440 EcoICRI GAG'CTC Cuts at: 0 681 693 1404 Size: 681 12 711 Fragments arranged by size: 711 681 12 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 13 436 448 619 634 929 1404 Size: 13 423 12 171 15 295 475 Fragments arranged by size: 475 423 295 171 15 13 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 31 943 1404 Size: 31 912 461 Fragments arranged by size: 912 461 31 EcoO109I rG'GnC_Cy Cuts at: 0 144 822 936 948 1062 1194 1404 Size: 144 678 114 12 114 132 210 Fragments arranged by size: 678 210 144 132 114 114 12 FspI TGC'GCA Cuts at: 0 754 1404 Size: 754 650 HaeII r_GCGC'y Cuts at: 0 126 240 348 668 972 1008 1404 Size: 126 114 108 320 304 36 396 Fragments arranged by size: 396 320 304 126 114 108 36 KasI G'GCGC_C Cuts at: 0 236 1404 Size: 236 1168 MluI A'CGCG_T Cuts at: 0 152 1404 Size: 152 1252 MspA1I CmG'CkG Cuts at: 0 381 522 585 704 711 930 1058 1117 Size: 381 141 63 119 7 219 128 59 Cuts at: 1117 1404 Size: 287 Fragments arranged by size: 381 287 219 141 128 119 63 59 7 NaeI GCC'GGC Cuts at: 0 1366 1404 Size: 1366 38 NarI GG'CG_CC Cuts at: 0 237 1404 Size: 237 1167 NgoMIV G'CCGG_C Cuts at: 0 1364 1404 Size: 1364 40 NotI GC'GGCC_GC Cuts at: 0 376 1404 Size: 376 1028 PasI CC'CwG_GG Cuts at: 0 1065 1404 Size: 1065 339 PfoI T'CCnGG_A Cuts at: 0 29 761 1190 1404 Size: 29 732 429 214 Fragments arranged by size: 732 429 214 29 PpuMI rG'GwC_Cy Cuts at: 0 822 936 1194 1404 Size: 822 114 258 210 Fragments arranged by size: 822 258 210 114 PshAI GACnn'nnGTC Cuts at: 0 490 1404 Size: 490 914 PspOMI G'GGCC_C Cuts at: 0 144 1061 1404 Size: 144 917 343 Fragments arranged by size: 917 343 144 PspXI vC'TCGA_Gb Cuts at: 0 694 1404 Size: 694 710 PstI C_TGCA'G Cuts at: 0 386 593 734 779 1404 Size: 386 207 141 45 625 Fragments arranged by size: 625 386 207 141 45 PvuII CAG'CTG Cuts at: 0 585 711 1058 1404 Size: 585 126 347 346 Fragments arranged by size: 585 347 346 126 SacI G_AGCT'C Cuts at: 0 683 695 1404 Size: 683 12 709 Fragments arranged by size: 709 683 12 SacII CC_GC'GG Cuts at: 0 1118 1404 Size: 1118 286 SanDI GG'GwC_CC Cuts at: 0 822 1194 1404 Size: 822 372 210 Fragments arranged by size: 822 372 210 SfcI C'TryA_G Cuts at: 0 382 589 730 775 1251 1404 Size: 382 207 141 45 476 153 Fragments arranged by size: 476 382 207 153 141 45 SfoI GGC'GCC Cuts at: 0 238 1404 Size: 238 1166 SmaI CCC'GGG Cuts at: 0 827 1404 Size: 827 577 SmlI C'TyrA_G Cuts at: 0 403 694 1404 Size: 403 291 710 Fragments arranged by size: 710 403 291 StyI C'CwwG_G Cuts at: 0 651 869 1178 1404 Size: 651 218 309 226 Fragments arranged by size: 651 309 226 218 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 100 526 1215 1404 Size: 100 426 689 189 Fragments arranged by size: 689 426 189 100 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1380 1404 Size: 1380 24 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1062 1404 Size: 1062 342 XhoI C'TCGA_G Cuts at: 0 694 1404 Size: 694 710 XmaI C'CCGG_G Cuts at: 0 825 1404 Size: 825 579 Enzymes that do cut: AarI AcuI AfeI AflIII AlwNI ApaI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspMI BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII KasI MluI MspA1I NaeI NarI NgoMIV NotI PasI PfoI PpuMI PshAI PspOMI PspXI PstI PvuII SacI SacII SanDI SfcI SfoI SmaI SmlI StyI TaqII TstI XcmI XhoI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI BaeI BcgI BciVI BclI BfrBI BglII BmtI BplI BsaAI BsmI BsmBI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I ClaI CspCI DraI DrdI EcoRI EcoRV FalI FseI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MmeI MscI MslI NcoI NdeI NheI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PpiI PsiI PsrI PvuI RsrII SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XmnI ZraI