################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## Acc65I AclI AflII AflIII AgeI AhdI Aor51HI ApaI AscI Asp718I AspEI AvaI AviII AvrII BbrPI BcgI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsiEI BsiWI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI CelII ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV FauI FseI FspI HincII HindII HindIII HpaI KpnI KspI MamI MluI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SfiI SfuI SgrAI SmaI SnaBI Sse8387I SspBI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AatII 2029 GACGTC GACGT^C AccI 422 GTATAC GT^[AC][GT]AC AccII 1560 CGCG CG^CG AccIII 663 TCCGGA T^CCGGA Alw44I 514 GTGCAC G^TGCAC AocI 247 CCTCAGG CC^T[ACGT]AGG ApaLI 514 GTGCAC G^TGCAC AseI 964 ATTAAT AT^TAAT AsnI 964 ATTAAT AT^TAAT Asp700I 85 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 2336 TGGCCA TGG^CCA BamHI 51 GGATCC G^GATCC BbeI 689 GGCGCC GGCGC^C BciVI 2528 GGATAC GTATCC(6/5) BclI 1772 TGATCA T^GATCA BcnI 2054 CCGGG CC^[GC]GG BglI 2017 GCCGCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 2592 GGTCTC GGTCTC(1/5) BsaWI 663 TCCGGA [AT]^CCGG[AT] BseAI 663 TCCGGA T^CCGGA BseRI 1181 GAGGAG GAGGAG(10/8) BsmBI 1571 GAGACG CGTCTC(1/5) BsmI 1959 GAATGC GAATGC(1/-1) BspEI 663 TCCGGA T^CCGGA BsrFI 557 ACCGGC [GA]^CCGG[CT] Bst1107I 422 GTATAC GTA^TAC BstUI 1560 CGCG CG^CG BstZ17I 422 GTATAC GTA^TAC Bsu36I 247 CCTCAGG CC^T[ACGT]AGG Cfr10I 557 ACCGGC [GA]^CCGG[CT] DrdI 394 GACATGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 247 CCTCAGG CC^T[ACGT]AGG EcoT22I 740 ATGCAT ATGCA^T FbaI 1772 TGATCA T^GATCA KasI 689 GGCGCC G^GCGCC MfeI 2654 CAATTG C^AATTG MluNI 2336 TGGCCA TGG^CCA MroI 663 TCCGGA T^CCGGA MscI 2336 TGGCCA TGG^CCA MunI 2654 CAATTG C^AATTG MvnI 1560 CGCG CG^CG NarI 689 GGCGCC GG^CGCC NciI 2054 CCGGG CC^[GC]GG NdeI 2378 CATATG CA^TATG NsiI 740 ATGCAT ATGCA^T PshBI 964 ATTAAT AT^TAAT SacI 2752 GAGCTC GAGCT^C SapI 2209 GCTCTTC GCTCTTC(1/4) ScaI 2143 AGTACT AGT^ACT SexAI 210 ACCTGGT A^CC[AT]GGT SfoI 689 GGCGCC GGC^GCC SmlI 1906 CTCAAG C^T[CT][GA]AG SpeI 1024 ACTAGT A^CTAGT StuI 28 AGGCCT AGG^CCT XmnI 85 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 803 AAATTT [GA]^AATT[CT] 2474 AAATTC AlwNI 980 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG 2769 CAGTGTCTG ApoI 803 AAATTT [GA]^AATT[CT] 2474 AAATTC AspI 1633 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2767 GACAGTGTC BanII 1411 GAGCCC G[GA]GC[CT]^C 2752 GAGCTC BbsI 2688 GAAGAC GAAGAC(2/6) 937 GTCTTC BbvCI 627 CCTCAGC CCTCAGC(-5/-2) 1806 GCTGAGG BpuAI 2688 GAAGAC GAAGAC(2/6) 937 GTCTTC BspMI 323 ACCTGC ACCTGC(4/8) 2349 GCAGGT EcoNI 2729 CCTCCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2890 CCTCTAGAAGG HaeII 689 GGCGCC [GA]GCGC^[CT] 1598 AGCGCC HgaI 712 GACGC GACGC(5/10) 1752 GACGC NcoI 198 CCATGG C^CATGG 1304 CCATGG PflFI 1633 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2767 GACAGTGTC PpuMI 1325 AGGACCC [GA]G^G[AT]CC[CT] 2886 AGGACCT PstI 604 CTGCAG CTGCA^G 2452 CTGCAG SfcI 604 CTGCAG C^T[GA][CT]AG 2452 CTGCAG SphI 327 GCATGC GCATG^C 1536 GCATGC TaqI 849 TCGA T^CGA 1553 TCGA TfiI 846 GATTC G^A[AT]TC 1315 GAATC Tth111I 1633 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2767 GACAGTGTC TthHB8I 849 TCGA T^CGA 1553 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AspHI AvaII BanI BbvI BfaI BmyI BpmI BsaHI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SspI StyI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XbaI XcmI XhoII