(Circular) (Six-base) MAPSORT of: pFN21AE2397.seq Check: 8695 from: 1 to: 7612 pFN21AE2397 7612 bp With 182 enzymes: SgfI * February 7, 2012 16:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2337 2337 Size: 7612 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4038 5422 278 Size: 53 83 186 936 2502 1384 2468 Fragments arranged by size: 2502 2468 1384 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2428 4955 1937 Size: 491 2527 4594 Fragments arranged by size: 4594 2527 491 Acc65I G'GTAC_C Cuts at: 4933 4933 Size: 7612 AclI AA'CG_TT Cuts at: 1563 5739 6112 1563 Size: 4176 373 3063 Fragments arranged by size: 4176 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3357 4464 5675 7059 7327 1632 Size: 1725 1107 1211 1384 268 1917 Fragments arranged by size: 1917 1725 1384 1211 1107 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7415 AflIII A'CryG_T Cuts at: 1203 6511 7488 1203 Size: 5308 977 1327 Fragments arranged by size: 5308 1327 977 AgeI A'CCGG_T Cuts at: 6443 6443 Size: 7612 AhdI GACnn_n'nnGTC Cuts at: 6341 6341 Size: 7612 AleI CACnn'nnGTG Cuts at: 1166 3617 1166 Size: 2451 5161 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3564 3888 4013 4088 1184 Size: 2380 324 125 75 4708 Fragments arranged by size: 4708 2380 324 125 75 AlwNI CAG_nnn'CTG Cuts at: 2990 4779 5355 6927 2990 Size: 1789 576 1572 3675 Fragments arranged by size: 3675 1789 1572 576 ApaLI G'TGCA_C Cuts at: 2519 5669 6825 2519 Size: 3150 1156 3306 Fragments arranged by size: 3306 3150 1156 ApoI r'AATT_y Cuts at: 1493 2808 4479 4921 5147 5299 7408 1493 Size: 1315 1671 442 226 152 2109 1697 Fragments arranged by size: 2109 1697 1671 1315 442 226 152 AseI AT'TA_AT Cuts at: 160 1032 2970 6166 160 Size: 872 1938 3196 1606 Fragments arranged by size: 3196 1938 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7612 AvaI C'yCGr_G Cuts at: 1870 1943 4937 1870 Size: 73 2994 4545 Fragments arranged by size: 4545 2994 73 BamHI G'GATC_C Cuts at: 2056 4942 5309 2056 Size: 2886 367 4359 Fragments arranged by size: 4359 2886 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2219 2694 3143 4841 4933 Size: 325 217 627 432 475 449 1698 92 Cuts at: 4933 6388 618 Size: 1455 1842 Fragments arranged by size: 1842 1698 1455 627 475 449 432 325 217 92 BanII G_rGCy'C Cuts at: 1391 3420 4761 4931 1391 Size: 2029 1341 170 4072 Fragments arranged by size: 4072 2029 1341 170 BbeI G_GCGC'C Cuts at: 2698 2698 Size: 7612 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2935 4700 928 Size: 971 1036 1765 3840 Fragments arranged by size: 3840 1765 1036 971 BbvCI CC'TCA_GC Cuts at: 2633 3812 2633 Size: 1179 6433 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5803 5837 1903 Size: 34 3866 34 3678 Fragments arranged by size: 3866 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4527 5504 6714 4527 Size: 977 1210 5425 Fragments arranged by size: 5425 1210 977 BclI T'GATC_A Cuts at: 1548 3777 1548 Size: 2229 5383 BfrBI ATG'CAT Cuts at: 2747 2747 Size: 7612 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4028 6223 136 Size: 107 122 71 631 605 2356 2195 1525 Fragments arranged by size: 2356 2195 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7607 7607 Size: 7612 BlpI GC'TnA_GC Cuts at: 5029 5029 Size: 7612 Bme1580I G_kGCm'C Cuts at: 1790 2523 3197 5673 6829 1790 Size: 733 674 2476 1156 2573 Fragments arranged by size: 2573 2476 1156 733 674 BmgBI CAC'GTC Cuts at: 1318 3580 1318 Size: 2262 5350 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4510 4682 5343 Size: 416 53 83 399 24 3093 172 661 Cuts at: 5343 6301 442 Size: 958 1753 Fragments arranged by size: 3093 1753 958 661 416 399 172 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7612 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4581 4581 Size: 7612 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2817 3174 3510 4145 4666 6272 1239 Size: 1578 357 336 635 521 1606 2579 Fragments arranged by size: 2579 1606 1578 635 521 357 336 Bpu10I CC'TnA_GC Cuts at: 2633 3812 3830 4044 2633 Size: 1179 18 214 6201 Fragments arranged by size: 6201 1179 214 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3896 5737 6602 6900 3896 Size: 1841 865 298 4608 Fragments arranged by size: 4608 1841 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4603 6275 882 Size: 617 3104 1672 2219 Fragments arranged by size: 3104 2219 1672 617 BsaAI yAC'GTr Cuts at: 493 1204 5403 493 Size: 711 4199 2702 Fragments arranged by size: 4199 2702 711 BsaBI GATnn'nnATC Cuts at: 7606 7606 Size: 7612 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2695 2718 3758 4035 Size: 53 83 186 936 1162 23 1040 277 Cuts at: 4035 5419 5801 6436 275 Size: 1384 382 635 1451 Fragments arranged by size: 1451 1384 1162 1040 936 635 382 277 186 83 53 23 BsaWI w'CCGG_w Cuts at: 2668 6045 6443 6717 6864 7275 2668 Size: 3377 398 274 147 411 3005 Fragments arranged by size: 3377 3005 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4040 4070 1178 Size: 30 165 30 2637 30 4720 Fragments arranged by size: 4720 2637 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3201 1186 Size: 2015 5597 BseYI C'CCAG_C Cuts at: 2569 3290 3419 3818 4025 4187 6815 7311 2569 Size: 721 129 399 207 162 2628 496 2870 Fragments arranged by size: 2870 2628 721 496 399 207 162 129 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2359 3104 3641 4058 1163 Size: 573 623 745 537 417 4717 Fragments arranged by size: 4717 745 623 573 537 417 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5062 5823 6851 1540 Size: 275 186 3061 761 1028 2301 Fragments arranged by size: 3061 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2523 4092 4761 5673 5758 6829 2523 Size: 1569 669 912 85 1071 3306 Fragments arranged by size: 3306 1569 1071 912 669 85 BsmI GAATG_Cn' Cuts at: 3970 5123 5216 3970 Size: 1153 93 6366 Fragments arranged by size: 6366 1153 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3570 3570 Size: 7612 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2523 3197 3420 4092 4761 4931 5673 Size: 399 733 674 223 672 669 170 742 Cuts at: 5673 5758 6829 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 733 674 672 669 399 223 170 85 BspEI T'CCGG_A Cuts at: 2668 7275 2668 Size: 4607 3005 BspHI T'CATG_A Cuts at: 5501 7231 5501 Size: 1730 5882 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2337 4345 4967 844 Size: 1493 2008 622 3489 Fragments arranged by size: 3489 2008 1493 622 BsrBI CCG'CTC Cuts at: 1394 1823 5499 1394 Size: 429 3676 3507 Fragments arranged by size: 3676 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6107 6281 65 Size: 1183 4859 174 1396 Fragments arranged by size: 4859 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2562 6256 6443 1700 Size: 110 752 3694 187 2869 Fragments arranged by size: 3694 2869 752 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7612 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7612 BssSI C'ACGA_G Cuts at: 5672 6684 5672 Size: 1012 6600 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7612 BstBI TT'CG_AA Cuts at: 6416 6416 Size: 7612 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7612 BstXI CCAn_nnnn'nTGG Cuts at: 2401 2507 2626 4207 4880 2401 Size: 106 119 1581 673 5133 Fragments arranged by size: 5133 1581 673 119 106 BstYI r'GATC_y Cuts at: 1972 2056 2370 3688 4237 4942 5309 5694 5711 Size: 84 314 1318 549 705 367 385 17 Cuts at: 5711 7163 7249 7261 7607 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1318 705 549 385 367 346 314 86 84 17 12 BstZ17I GTA'TAC Cuts at: 2429 2429 Size: 7612 Bsu36I CC'TnA_GG Cuts at: 2253 2253 Size: 7612 BtgI C'CryG_G Cuts at: 513 1065 2004 2203 2706 3309 513 Size: 552 939 199 503 603 4816 Fragments arranged by size: 4816 939 603 552 503 199 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6873 Fragments arranged by size: 6873 683 56 BtsI GCAGTG_nn' Cuts at: 2111 3612 3780 4070 4847 5128 5922 5942 2111 Size: 1501 168 290 777 281 794 20 3781 Fragments arranged by size: 3781 1501 794 777 290 281 168 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7612 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7577 DraI TTT'AAA Cuts at: 4916 4916 Size: 7612 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2405 6619 809 Size: 507 1089 4214 1802 Fragments arranged by size: 4214 1802 1089 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3594 4020 4341 Size: 54 1425 50 275 18 1764 426 321 Cuts at: 4341 5059 5948 7577 8 Size: 718 889 1629 43 Fragments arranged by size: 1764 1629 1425 889 718 426 321 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5059 1537 Size: 275 3247 4090 Fragments arranged by size: 4090 3247 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2859 3474 4221 5542 1353 Size: 1506 615 747 1321 3423 Fragments arranged by size: 3423 1506 1321 747 615 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2585 3174 6199 6573 6719 387 Size: 1064 138 996 589 3025 374 146 1280 Fragments arranged by size: 3025 1280 1064 996 589 374 146 138 EcoICRI GAG'CTC Cuts at: 4759 4759 Size: 7612 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2817 3174 3357 3510 4145 4464 4666 Size: 393 1185 357 183 153 635 319 202 Cuts at: 4666 5675 6272 7059 7327 1239 Size: 1009 597 787 268 1524 Fragments arranged by size: 1524 1185 1009 787 635 597 393 357 319 268 202 183 153 EcoNI CCTnn'n_nnAGG Cuts at: 4738 4899 4738 Size: 161 7451 EcoO109I rG'GnC_Cy Cuts at: 3331 4630 4892 3331 Size: 1299 262 6051 Fragments arranged by size: 6051 1299 262 EcoRI G'AATT_C Cuts at: 4921 5299 4921 Size: 378 7234 FspI TGC'GCA Cuts at: 6118 6118 Size: 7612 HaeII r_GCGC'y Cuts at: 1678 2698 3607 6759 1678 Size: 1020 909 3152 2531 Fragments arranged by size: 3152 2531 1020 909 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3079 3111 3305 3337 3730 Size: 32 135 32 1507 32 194 32 393 Cuts at: 3730 3762 4040 4072 4085 4117 4385 4417 4687 Size: 32 278 32 13 32 268 32 270 Cuts at: 4687 4719 6253 6285 6327 6359 1373 Size: 32 1534 32 42 32 2626 Fragments arranged by size: 2626 1534 1507 393 278 270 268 194 135 42 32 32 32 32 32 32 32 32 32 32 32 13 HincII GTy'rAC Cuts at: 669 1657 1938 4956 5202 669 Size: 988 281 3018 246 3079 Fragments arranged by size: 3079 3018 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7612 HpaI GTT'AAC Cuts at: 5202 5202 Size: 7612 KasI G'GCGC_C Cuts at: 2694 2694 Size: 7612 KpnI G_GTAC'C Cuts at: 4937 4937 Size: 7612 MfeI C'AATT_G Cuts at: 4659 5211 4659 Size: 552 7060 MluI A'CGCG_T Cuts at: 7488 7488 Size: 7612 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2775 6726 6910 1305 Size: 1470 3951 184 2007 Fragments arranged by size: 3951 2007 1470 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4343 10 Size: 54 1425 343 2511 3279 Fragments arranged by size: 3279 2511 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2331 3546 3617 4878 5570 5929 6088 Size: 648 1165 1215 71 1261 692 359 159 Cuts at: 6088 518 Size: 2042 Fragments arranged by size: 2042 1261 1215 1165 692 648 359 159 71 MspA1I CmG'CkG Cuts at: 1606 2569 2783 4025 4187 4350 4438 4462 4788 Size: 963 214 1242 162 163 88 24 326 Cuts at: 4788 5028 5705 6853 7098 1606 Size: 240 677 1148 245 2120 Fragments arranged by size: 2120 1242 1148 963 677 326 245 240 214 163 162 88 24 NarI GG'CG_CC Cuts at: 2695 2695 Size: 7612 NcoI C'CATG_G Cuts at: 513 1065 2004 2203 3309 513 Size: 552 939 199 1106 4816 Fragments arranged by size: 4816 1106 939 552 199 NdeI CA'TA_TG Cuts at: 387 4384 387 Size: 3997 3615 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7612 NotI GC'GGCC_GC Cuts at: 5059 5059 Size: 7612 NsiI A_TGCA'T Cuts at: 2749 2749 Size: 7612 NspI r_CATG'y Cuts at: 1132 1229 2336 3169 3545 4970 6515 1132 Size: 97 1107 833 376 1425 1545 2229 Fragments arranged by size: 2229 1545 1425 1107 833 376 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7612 PciI A'CATG_T Cuts at: 6511 6511 Size: 7612 PflMI CCAn_nnn'nTGG Cuts at: 1108 2204 2344 3233 4350 1108 Size: 1096 140 889 1117 4370 Fragments arranged by size: 4370 1117 1096 889 140 PfoI T'CCnGG_A Cuts at: 2506 3268 4122 2506 Size: 762 854 5996 Fragments arranged by size: 5996 854 762 PmeI GTTT'AAAC Cuts at: 4916 4916 Size: 7612 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4315 4347 5681 5713 7194 7226 4315 Size: 32 1334 32 1481 32 4701 Fragments arranged by size: 4701 1481 1334 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3331 4892 3331 Size: 1561 6051 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7612 PsiI TTA'TAA Cuts at: 5182 5182 Size: 7612 PstI C_TGCA'G Cuts at: 830 2613 4461 4964 830 Size: 1783 1848 503 3478 Fragments arranged by size: 3478 1848 1783 503 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7612 PvuII CAG'CTG Cuts at: 2569 2783 4187 4350 4438 4462 2569 Size: 214 1404 163 88 24 5719 Fragments arranged by size: 5719 1404 214 163 88 24 SacI G_AGCT'C Cuts at: 4761 4761 Size: 7612 SalI G'TCGA_C Cuts at: 1936 4954 1936 Size: 3018 4594 SapI GCTCTTCn'nnn_ Cuts at: 4221 4221 Size: 7612 SbfI CC_TGCA'GG Cuts at: 4964 4964 Size: 7612 ScaI AGT'ACT Cuts at: 4150 5860 7572 4150 Size: 1710 1712 4190 Fragments arranged by size: 4190 1712 1710 SexAI A'CCwGG_T Cuts at: 2215 2215 Size: 7612 SfcI C'TryA_G Cuts at: 826 1045 2609 4457 4960 6095 6776 6967 826 Size: 219 1564 1848 503 1135 681 191 1471 Fragments arranged by size: 1848 1564 1471 1135 681 503 219 191 SfoI GGC'GCC Cuts at: 2696 2696 Size: 7612 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7612 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7612 SmaI CCC'GGG Cuts at: 1872 4939 1872 Size: 3067 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3911 5716 6617 6879 820 Size: 197 926 1968 1805 901 262 1553 Fragments arranged by size: 1968 1805 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5403 493 Size: 4910 2702 SpeI A'CTAG_T Cuts at: 152 3029 152 Size: 2877 4735 SphI G_CATG'C Cuts at: 1132 2336 3545 4970 1132 Size: 1204 1209 1425 3774 Fragments arranged by size: 3774 1425 1209 1204 SspI AAT'ATT Cuts at: 5 52 2079 2145 2974 5536 5 Size: 47 2027 66 829 2562 2081 Fragments arranged by size: 2562 2081 2027 829 66 47 StuI AGG'CCT Cuts at: 1517 2035 1517 Size: 518 7094 StyI C'CwwG_G Cuts at: 513 1065 2004 2203 2394 2619 3309 5051 513 Size: 552 939 199 191 225 690 1742 3074 Fragments arranged by size: 3074 1742 939 690 552 225 199 191 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5639 5656 5809 5994 6439 325 Size: 1255 4059 17 153 185 445 1498 Fragments arranged by size: 4059 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3203 4148 5858 Size: 274 80 33 51 1443 1226 945 1710 Cuts at: 5858 7570 96 Size: 1712 138 Fragments arranged by size: 1712 1710 1443 1226 945 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2502 2534 7572 7604 2502 Size: 32 5038 32 2510 Fragments arranged by size: 5038 2510 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3641 4775 1316 Size: 2325 1134 4153 Fragments arranged by size: 4153 2325 1134 XbaI T'CTAG_A Cuts at: 3511 4333 4897 4948 3511 Size: 822 564 51 6175 Fragments arranged by size: 6175 822 564 51 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2401 4497 4579 2401 Size: 2096 82 5434 Fragments arranged by size: 5434 2096 82 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7612 XmaI C'CCGG_G Cuts at: 1870 4937 1870 Size: 3067 4545 XmnI GAAnn'nnTTC Cuts at: 2094 5741 2094 Size: 3647 3965 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4036 5420 276 Size: 53 83 186 936 2502 1384 2468 Fragments arranged by size: 2502 2468 1384 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BsiWI DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI