(Linear) (Six-base) MAPSORT of: KIEE2397.seq Check: 5541 from: 1 to: 2907 KIEE2397.orf With 182 enzymes: SgfI * February 7, 2012 15:11 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 332 2907 Size: 332 2575 AatII G_ACGT'C Cuts at: 0 2033 2907 Size: 2033 874 AccI GT'mk_AC Cuts at: 0 423 2907 Size: 423 2484 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1352 2459 2907 Size: 1352 1107 448 Fragments arranged by size: 1352 1107 448 AleI CACnn'nnGTG Cuts at: 0 1612 2907 Size: 1612 1295 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1559 1883 2008 2083 2907 Size: 1559 324 125 75 824 Fragments arranged by size: 1559 824 324 125 75 AlwNI CAG_nnn'CTG Cuts at: 0 985 2774 2907 Size: 985 1789 133 Fragments arranged by size: 1789 985 133 ApaLI G'TGCA_C Cuts at: 0 514 2907 Size: 514 2393 ApoI r'AATT_y Cuts at: 0 803 2474 2907 Size: 803 1671 433 Fragments arranged by size: 1671 803 433 AseI AT'TA_AT Cuts at: 0 965 2907 Size: 965 1942 BamHI G'GATC_C Cuts at: 0 51 2907 Size: 51 2856 BanI G'GyrC_C Cuts at: 0 214 689 1138 2836 2907 Size: 214 475 449 1698 71 Fragments arranged by size: 1698 475 449 214 71 BanII G_rGCy'C Cuts at: 0 1415 2756 2907 Size: 1415 1341 151 Fragments arranged by size: 1415 1341 151 BbeI G_GCGC'C Cuts at: 0 693 2907 Size: 693 2214 BbsI GAAGACnn'nnnn_ Cuts at: 0 930 2695 2907 Size: 930 1765 212 Fragments arranged by size: 1765 930 212 BbvCI CC'TCA_GC Cuts at: 0 628 1807 2907 Size: 628 1179 1100 Fragments arranged by size: 1179 1100 628 BciVI GTATCCnnnnn_n' Cuts at: 0 2522 2907 Size: 2522 385 BclI T'GATC_A Cuts at: 0 1772 2907 Size: 1772 1135 BfrBI ATG'CAT Cuts at: 0 742 2907 Size: 742 2165 BglI GCCn_nnn'nGGC Cuts at: 0 2023 2907 Size: 2023 884 Bme1580I G_kGCm'C Cuts at: 0 518 1192 2907 Size: 518 674 1715 Fragments arranged by size: 1715 674 518 BmgBI CAC'GTC Cuts at: 0 1575 2907 Size: 1575 1332 BmrI ACTGGGnnnn_n' Cuts at: 0 2505 2677 2907 Size: 2505 172 230 Fragments arranged by size: 2505 230 172 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2576 2907 Size: 2576 331 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 812 1169 1505 2140 2661 2907 Size: 812 357 336 635 521 246 Fragments arranged by size: 812 635 521 357 336 246 Bpu10I CC'TnA_GC Cuts at: 0 628 1807 1825 2039 2907 Size: 628 1179 18 214 868 Fragments arranged by size: 1179 868 628 214 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1891 2907 Size: 1891 1016 BsaI GGTCTCn'nnnn_ Cuts at: 0 2598 2907 Size: 2598 309 BsaHI Gr'CG_yC Cuts at: 0 690 713 1753 2030 2907 Size: 690 23 1040 277 877 Fragments arranged by size: 1040 877 690 277 23 BsaWI w'CCGG_w Cuts at: 0 663 2907 Size: 663 2244 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2035 2065 2907 Size: 2035 30 842 Fragments arranged by size: 2035 842 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1196 2907 Size: 1196 1711 BseYI C'CCAG_C Cuts at: 0 564 1285 1414 1813 2020 2182 2907 Size: 564 721 129 399 207 162 725 Fragments arranged by size: 725 721 564 399 207 162 129 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 354 1099 1636 2053 2907 Size: 354 745 537 417 854 Fragments arranged by size: 854 745 537 417 354 BsiHKAI G_wGCw'C Cuts at: 0 518 2087 2756 2907 Size: 518 1569 669 151 Fragments arranged by size: 1569 669 518 151 BsmI GAATG_Cn' Cuts at: 0 1965 2907 Size: 1965 942 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1565 2907 Size: 1565 1342 Bsp1286I G_dGCh'C Cuts at: 0 518 1192 1415 2087 2756 2907 Size: 518 674 223 672 669 151 Fragments arranged by size: 674 672 669 518 223 151 BspEI T'CCGG_A Cuts at: 0 663 2907 Size: 663 2244 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 332 2340 2907 Size: 332 2008 567 Fragments arranged by size: 2008 567 332 BsrFI r'CCGG_y Cuts at: 0 557 2907 Size: 557 2350 BstXI CCAn_nnnn'nTGG Cuts at: 0 396 502 621 2202 2875 2907 Size: 396 106 119 1581 673 32 Fragments arranged by size: 1581 673 396 119 106 32 BstYI r'GATC_y Cuts at: 0 51 365 1683 2232 2907 Size: 51 314 1318 549 675 Fragments arranged by size: 1318 675 549 314 51 BstZ17I GTA'TAC Cuts at: 0 424 2907 Size: 424 2483 Bsu36I CC'TnA_GG Cuts at: 0 248 2907 Size: 248 2659 BtgI C'CryG_G Cuts at: 0 198 701 1304 2907 Size: 198 503 603 1603 Fragments arranged by size: 1603 603 503 198 BtsI GCAGTG_nn' Cuts at: 0 106 1607 1775 2065 2842 2907 Size: 106 1501 168 290 777 65 Fragments arranged by size: 1501 777 290 168 106 65 DrdI GACnn_nn'nnGTC Cuts at: 0 400 2907 Size: 400 2507 EaeI y'GGCC_r Cuts at: 0 1589 2015 2336 2907 Size: 1589 426 321 571 Fragments arranged by size: 1589 571 426 321 EarI CTCTTCn'nnn_ Cuts at: 0 854 1469 2216 2907 Size: 854 615 747 691 Fragments arranged by size: 854 747 691 615 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 580 1169 2907 Size: 580 589 1738 Fragments arranged by size: 1738 589 580 EcoICRI GAG'CTC Cuts at: 0 2754 2907 Size: 2754 153 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 812 1169 1352 1505 2140 2459 2661 2907 Size: 812 357 183 153 635 319 202 246 Fragments arranged by size: 812 635 357 319 246 202 183 153 EcoNI CCTnn'n_nnAGG Cuts at: 0 2733 2894 2907 Size: 2733 161 13 Fragments arranged by size: 2733 161 13 EcoO109I rG'GnC_Cy Cuts at: 0 1326 2625 2887 2907 Size: 1326 1299 262 20 Fragments arranged by size: 1326 1299 262 20 HaeII r_GCGC'y Cuts at: 0 693 1602 2907 Size: 693 909 1305 Fragments arranged by size: 1305 909 693 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1074 1106 1300 1332 1725 1757 2035 2067 Size: 1074 32 194 32 393 32 278 32 Cuts at: 2067 2080 2112 2380 2412 2682 2714 2907 Size: 13 32 268 32 270 32 193 Fragments arranged by size: 1074 393 278 270 268 194 193 32 32 32 32 32 32 32 13 KasI G'GCGC_C Cuts at: 0 689 2907 Size: 689 2218 MfeI C'AATT_G Cuts at: 0 2654 2907 Size: 2654 253 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 770 2907 Size: 770 2137 MscI TGG'CCA Cuts at: 0 2338 2907 Size: 2338 569 MslI CAynn'nnrTG Cuts at: 0 326 1541 1612 2873 2907 Size: 326 1215 71 1261 34 Fragments arranged by size: 1261 1215 326 71 34 MspA1I CmG'CkG Cuts at: 0 564 778 2020 2182 2345 2433 2457 2783 Size: 564 214 1242 162 163 88 24 326 Cuts at: 2783 2907 Size: 124 Fragments arranged by size: 1242 564 326 214 163 162 124 88 24 NarI GG'CG_CC Cuts at: 0 690 2907 Size: 690 2217 NcoI C'CATG_G Cuts at: 0 198 1304 2907 Size: 198 1106 1603 Fragments arranged by size: 1603 1106 198 NdeI CA'TA_TG Cuts at: 0 2379 2907 Size: 2379 528 NsiI A_TGCA'T Cuts at: 0 744 2907 Size: 744 2163 NspI r_CATG'y Cuts at: 0 331 1164 1540 2907 Size: 331 833 376 1367 Fragments arranged by size: 1367 833 376 331 PflMI CCAn_nnn'nTGG Cuts at: 0 199 339 1228 2345 2907 Size: 199 140 889 1117 562 Fragments arranged by size: 1117 889 562 199 140 PfoI T'CCnGG_A Cuts at: 0 501 1263 2117 2907 Size: 501 762 854 790 Fragments arranged by size: 854 790 762 501 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2310 2342 2907 Size: 2310 32 565 Fragments arranged by size: 2310 565 32 PpuMI rG'GwC_Cy Cuts at: 0 1326 2887 2907 Size: 1326 1561 20 Fragments arranged by size: 1561 1326 20 PstI C_TGCA'G Cuts at: 0 608 2456 2907 Size: 608 1848 451 Fragments arranged by size: 1848 608 451 PvuII CAG'CTG Cuts at: 0 564 778 2182 2345 2433 2457 2907 Size: 564 214 1404 163 88 24 450 Fragments arranged by size: 1404 564 450 214 163 88 24 SacI G_AGCT'C Cuts at: 0 2756 2907 Size: 2756 151 SapI GCTCTTCn'nnn_ Cuts at: 0 2216 2907 Size: 2216 691 ScaI AGT'ACT Cuts at: 0 2145 2907 Size: 2145 762 SexAI A'CCwGG_T Cuts at: 0 210 2907 Size: 210 2697 SfcI C'TryA_G Cuts at: 0 604 2452 2907 Size: 604 1848 455 Fragments arranged by size: 1848 604 455 SfoI GGC'GCC Cuts at: 0 691 2907 Size: 691 2216 SmlI C'TyrA_G Cuts at: 0 1906 2907 Size: 1906 1001 SpeI A'CTAG_T Cuts at: 0 1024 2907 Size: 1024 1883 SphI G_CATG'C Cuts at: 0 331 1540 2907 Size: 331 1209 1367 Fragments arranged by size: 1367 1209 331 SspI AAT'ATT Cuts at: 0 74 140 969 2907 Size: 74 66 829 1938 Fragments arranged by size: 1938 829 74 66 StuI AGG'CCT Cuts at: 0 30 2907 Size: 30 2877 StyI C'CwwG_G Cuts at: 0 198 389 614 1304 2907 Size: 198 191 225 690 1603 Fragments arranged by size: 1603 690 225 198 191 TatI w'GTAC_w Cuts at: 0 1198 2143 2907 Size: 1198 945 764 Fragments arranged by size: 1198 945 764 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 497 529 2907 Size: 497 32 2378 Fragments arranged by size: 2378 497 32 Tth111I GACn'n_nGTC Cuts at: 0 1636 2770 2907 Size: 1636 1134 137 Fragments arranged by size: 1636 1134 137 XbaI T'CTAG_A Cuts at: 0 1506 2328 2892 2907 Size: 1506 822 564 15 Fragments arranged by size: 1506 822 564 15 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 396 2492 2574 2907 Size: 396 2096 82 333 Fragments arranged by size: 2096 396 333 82 XmnI GAAnn'nnTTC Cuts at: 0 89 2907 Size: 89 2818 ZraI GAC'GTC Cuts at: 0 2031 2907 Size: 2031 876 Enzymes that do cut: AarI AatII AccI AcuI AleI AlfI AlwNI ApaLI ApoI AseI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrFI BstXI BstYI BstZ17I Bsu36I BtgI BtsI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MfeI MmeI MscI MslI MspA1I NarI NcoI NdeI NsiI NspI PflMI PfoI PpiI PpuMI PstI PvuII SacI SapI ScaI SexAI SfcI SfoI SmlI SpeI SphI SspI StuI StyI TatI TstI Tth111I XbaI XcmI XmnI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI ApaI AscI AsiSI AvaI AvrII BaeI BcgI BglII BlpI BmtI BsaAI BsaBI BsiEI BsiWI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BtgZI ClaI CspCI DraI DraIII EagI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MluI NaeI NgoMIV NheI NotI NruI PacI PasI PciI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI SfiI SgfI SgrAI SmaI SnaBI SrfI SwaI TaqII XhoI XmaI