################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluI MluNI MroI MscI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp509I Tth111I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AflIII 126 ACATGT A^C[GA][CT]GT AlwI 592 GATCC GGATC(4/5) AocI 63 CCTGAGG CC^T[ACGT]AGG Asp700I 315 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbsI 681 GAAGAC GAAGAC(2/6) BcnI 198 CCGGG CC^[GC]GG BpmI 34 CTGGAG CTGGAG(16/14) BpuAI 681 GAAGAC GAAGAC(2/6) BsgI 163 CTGCAC GTGCAG(16/14) BsmI 290 GCATTC GAATGC(1/-1) BspLU11I 126 ACATGT A^CATGT Bsu36I 63 CCTGAGG CC^T[ACGT]AGG DraII 432 AGGTCCT [GA]G^G[ACGT]CC[CT] DsaI 134 CCATGG C^C[GA][CT]GG EarI 202 GAAGAG CTCTTC(1/4) Eco81I 63 CCTGAGG CC^T[ACGT]AGG EcoO109I 432 AGGTCCT [GA]G^G[ACGT]CC[CT] HapII 198 CCGG C^CGG HgaI 554 GCGTC GACGC(5/10) HincII 29 GTCAAC GT[CT]^[GA]AC HindII 29 GTCAAC GT[CT]^[GA]AC HpaII 198 CCGG C^CGG Ksp632I 202 GAAGAG CTCTTC(1/4) MslI 933 CACTTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 732 CAGCTG C[AC]G^C[GT]G MspI 198 CCGG C^CGG NciI 198 CCGGG CC^[GC]GG NcoI 134 CCATGG C^CATGG PpuMI 432 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 878 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 118 CTGCAG CTGCA^G PvuII 732 CAGCTG CAG^CTG SacI 193 GAGCTC GAGCT^C SfaNI 153 GCATC GCATC(5/9) SfcI 118 CTGCAG C^T[GA][CT]AG StuI 483 AGGCCT AGG^CCT Tsp45I 566 GTGAC ^GT[GC]AC TspRI 827 CACTG CA[GC]TG[ACGT][ACGT]^ XcmI 272 CCAAGGATATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 315 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AspHI 193 GAGCTC G[AT]GC[AT]^C 532 GTGCTC AvaII 433 GGTCC G^G[AT]CC 504 GGACC BanII 193 GAGCTC G[GA]GC[CT]^C 627 GGGCTC BsiHKAI 193 GAGCTC G[AT]GC[AT]^C 532 GTGCTC BsrDI 1001 CATTGC GCAATG(2/0) 258 GCAATG BstF5I 18 CATCC GGATG(2/0) 1015 CATCC DpnII 592 GATC ^GATC 790 GATC EcoNI 748 CCTGAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 840 CCTCGACAAGG EcoT14I 134 CCATGG C^C[AT][AT]GG 272 CCAAGG FokI 18 CATCC GGATG(9/13) 1015 CATCC HaeIII 421 GGCC GG^CC 484 GGCC MaeIII 566 GTGAC ^GT[ACGT]AC 929 GTAAC MboI 592 GATC ^GATC 790 GATC NdeII 592 GATC ^GATC 790 GATC NlaIV 504 GGACCC GG[ACGT]^[ACGT]CC 779 GGAACC NspI 71 ACATGC [GA]CATG^[CT] 126 ACATGT PflMI 441 CCAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 551 CCAGCGTCTGG PleI 607 GACTC GAGTC(4/5) 909 GACTC Sau3AI 592 GATC ^GATC 790 GATC SmlI 609 CTCAAG C^T[CT][GA]AG 630 CTCAAG StyI 134 CCATGG C^C[AT][AT]GG 272 CCAAGG TaqI 323 TCGA T^CGA 842 TCGA TthHB8I 323 TCGA T^CGA 842 TCGA Van91I 441 CCAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 551 CCAGCGTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AfaI AluI AlwNI BbvI BmyI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI EcoRII Fnu4HI HinfI HphI ItaI MboII MnlI MseI MvaI MwoI NlaIII RsaI Sau96I ScrFI TfiI Tru9I TseI