Restriction map for
pFN21AB5915
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
Acc65I AccIII AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AvrII BamHI BbrPI BbsI BclI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BseAI BsiHKAI BsiWI
Bsp1407I BspDI BspEI BsrDI BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI DsaI Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI HindIII HpaI KpnI
KspI MamI MfeI MluI MroI
MslI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI PvuI RsrII SacI SacII
SapI SfiI SfuI SmaI SnaBI
SpeI SspBI SspI StyI SwaI
TfiI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 1380 GTCGAC GT^[AC][GT]AC
AclI 937 AACGTT AA^CGTT
Asp700I 1438 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 715 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 810 TGCGCA TGC^GCA
BalI 695 TGGCCA TGG^CCA
BanI 981 GGCGCC G^G[CT][GA]CC
BbeI 981 GGCGCC GGCGC^C
BcgI 957 CGAGCAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1051 GGATAC GTATCC(6/5)
BfaI 791 CTAG C^TAG
BglI 211 GCCGGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 800 AGATCT A^GATCT
BsaWI 1067 ACCGGA [AT]^CCGG[AT]
BsiEI 5 CGGCCG CG[GA][CT]^CG
BsmBI 1367 GAGACG CGTCTC(1/5)
BsmI 813 GCATTC GAATGC(1/-1)
BspHI 1358 TCATGA T^CATGA
BsrBI 102 CCGCTC CCGCTC(-3/-3)
BssHII 173 GCGCGC G^CGCGC
BstXI 992 CCAATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 5 CGGCCG C^GGCCG
EclXI 5 CGGCCG C^GGCCG
Eco52I 5 CGGCCG C^GGCCG
FspI 810 TGCGCA TGC^GCA
HaeII 981 GGCGCC [GA]GCGC^[CT]
HgaI 1113 GACGC GACGC(5/10)
HincII 1380 GTCGAC GT[CT]^[GA]AC
HindII 1380 GTCGAC GT[CT]^[GA]AC
HinfI 576 GACTC G^A[ACGT]TC
KasI 981 GGCGCC G^GCGCC
MaeI 791 CTAG C^TAG
MluNI 695 TGGCCA TGG^CCA
MscI 695 TGGCCA TGG^CCA
NarI 981 GGCGCC GG^CGCC
PflFI 715 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 576 GACTC GAGTC(4/5)
PpuMI 612 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 576 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 937 AACGTT AA^CGTT
RcaI 1358 TCATGA T^CATGA
SalI 1380 GTCGAC G^TCGAC
ScaI 1088 AGTACT AGT^ACT
SexAI 716 ACCTGGT A^CC[AT]GGT
SfoI 981 GGCGCC GGC^GCC
SgrAI 210 CGCCGGCG C[GA]^CCGG[CT]G
SphI 110 GCATGC GCATG^C
StuI 440 AGGCCT AGG^CCT
TspRI 1279 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 715 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1175 CCATCTACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1438 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AatII 1132 GACGTC GACGT^C
1369 GACGTC
AcsI 972 GAATTT [GA]^AATT[CT]
1034 AAATTT
AlwI 24 GATCC GGATC(4/5)
785 GGATC
ApoI 972 GAATTT [GA]^AATT[CT]
1034 AAATTT
AvaI 595 CTCGGG C^[CT]CG[GA]G
955 CCCGAG
AvaII 36 GGACC G^G[AT]CC
613 GGACC
BbvCI 168 CCTCAGC CCTCAGC(-5/-2)
552 GCTGAGG
BsoBI 595 CTCGGG C^[CT]CG[GA]G
955 CCCGAG
BspLU11I 1106 ACATGT A^CATGT
1126 ACATGT
BsrFI 136 GCCGGC [GA]^CCGG[CT]
211 GCCGGC
BstAPI 153 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
354 GCAGCTGCTGC
BstYI 23 AGATCC [GA]^GATC[CT]
800 AGATCT
Cfr10I 136 GCCGGC [GA]^CCGG[CT]
211 GCCGGC
DraIII 472 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
1396 CACCGAGTG
EaeI 5 CGGCCG [CT]^GGCC[GA]
695 TGGCCA
MflI 23 AGATCC [GA]^GATC[CT]
800 AGATCT
MseI 896 TTAA T^TAA
1319 TTAA
NaeI 136 GCCGGC GCC^GGC
211 GCCGGC
NgoMIV 136 GCCGGC G^CCGGC
211 GCCGGC
PvuII 157 CAGCTG CAG^CTG
355 CAGCTG
SbfI 333 CCTGCAGG CCTGCA^GG
432 CCTGCAGG
SfaNI 964 GATGC GCATC(5/9)
1349 GCATC
SmlI 1291 CTCAAG C^T[CT][GA]AG
1408 CTCAAG
Sse8387I 333 CCTGCAGG CCTGCA^GG
432 CCTGCAGG
TaqI 71 TCGA T^CGA
1381 TCGA
Tru9I 896 TTAA T^TAA
1319 TTAA
TthHB8I 71 TCGA T^CGA
1381 TCGA
XhoII 23 AGATCC [GA]^GATC[CT]
800 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwNI ApaI BanII BbvI
BcnI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BssSI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EarI EcoNI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PstI RsaI
Sau3AI Sau96I ScrFI SfcI TseI
Tsp45I Tsp509I Van91I