(Circular) (Six-base) MAPSORT of: pFN21AB5915.seq Check: 8039 from: 1 to: 6220 pFN21AB5915 6220 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2407 2407 Size: 6220 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3141 3378 4030 278 Size: 53 83 186 936 1605 237 652 2468 Fragments arranged by size: 2468 1605 936 652 237 186 83 53 AccI GT'mk_AC Cuts at: 1937 3386 3563 1937 Size: 1449 177 4594 Fragments arranged by size: 4594 1449 177 Acc65I G'GTAC_C Cuts at: 3541 3541 Size: 6220 AclI AA'CG_TT Cuts at: 1563 2943 4347 4720 1563 Size: 1380 1404 373 3063 Fragments arranged by size: 3063 1404 1380 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2567 4283 5667 5935 1632 Size: 935 1716 1384 268 1917 Fragments arranged by size: 1917 1716 1384 935 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6023 AflIII A'CryG_T Cuts at: 1203 3111 3131 3306 5119 6096 1203 Size: 1908 20 175 1813 977 1327 Fragments arranged by size: 1908 1813 1327 977 175 20 AgeI A'CCGG_T Cuts at: 5051 5051 Size: 6220 AhdI GACnn_n'nnGTC Cuts at: 4949 4949 Size: 6220 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6220 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6220 AlwNI CAG_nnn'CTG Cuts at: 2164 2365 2557 3963 5535 2164 Size: 201 192 1406 1572 2849 Fragments arranged by size: 2849 1572 1406 201 192 ApaI G_GGCC'C Cuts at: 2227 2249 3219 2227 Size: 22 970 5228 Fragments arranged by size: 5228 970 22 ApaLI G'TGCA_C Cuts at: 4277 5433 4277 Size: 1156 5064 ApoI r'AATT_y Cuts at: 1493 2977 3039 3529 3755 3907 6016 1493 Size: 1484 62 490 226 152 2109 1697 Fragments arranged by size: 2109 1697 1484 490 226 152 62 AseI AT'TA_AT Cuts at: 160 1032 4774 160 Size: 872 3742 1606 Fragments arranged by size: 3742 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 AvaI C'yCGr_G Cuts at: 1870 1943 2600 2960 3545 1870 Size: 73 657 360 585 4545 Fragments arranged by size: 4545 657 585 360 73 BamHI G'GATC_C Cuts at: 3550 3917 3550 Size: 367 5853 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2986 3541 4996 618 Size: 325 217 627 1199 555 1455 1842 Fragments arranged by size: 1842 1455 1199 627 555 325 217 BanII G_rGCy'C Cuts at: 1391 2227 2249 2652 3219 3539 1391 Size: 836 22 403 567 320 4072 Fragments arranged by size: 4072 836 567 403 320 22 BbeI G_GCGC'C Cuts at: 2990 2990 Size: 6220 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5249 BbvCI CC'TCA_GC Cuts at: 2174 2558 2174 Size: 384 5836 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2951 2985 4411 4445 1903 Size: 34 1014 34 1426 34 3678 Fragments arranged by size: 3678 1426 1014 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3050 4112 5322 3050 Size: 1062 1210 3948 Fragments arranged by size: 3948 1210 1062 BclI T'GATC_A Cuts at: 1548 1548 Size: 6220 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2222 4831 Size: 107 122 71 631 605 337 213 2609 Cuts at: 4831 136 Size: 1525 Fragments arranged by size: 2609 1525 631 605 337 213 122 107 71 BglII A'GATC_T Cuts at: 2805 6215 2805 Size: 3410 2810 BlpI GC'TnA_GC Cuts at: 3637 3637 Size: 6220 Bme1580I G_kGCm'C Cuts at: 1790 2227 2249 3219 4281 5437 1790 Size: 437 22 970 1062 1156 2573 Fragments arranged by size: 2573 1156 1062 970 437 22 BmgBI CAC'GTC Cuts at: 1318 2349 3309 1318 Size: 1031 960 4229 Fragments arranged by size: 4229 1031 960 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2491 2586 3951 Size: 416 53 83 399 24 1074 95 1365 Cuts at: 3951 4909 442 Size: 958 1753 Fragments arranged by size: 1753 1365 1074 958 416 399 95 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6220 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2347 3428 2347 Size: 1081 5139 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2057 2417 2514 2834 4880 1239 Size: 818 360 97 320 2046 2579 Fragments arranged by size: 2579 2046 818 360 320 97 Bpu10I CC'TnA_GC Cuts at: 2129 2174 2558 2663 2129 Size: 45 384 105 5686 Fragments arranged by size: 5686 384 105 45 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3281 3398 4345 5210 5508 3281 Size: 117 947 865 298 3993 Fragments arranged by size: 3993 947 865 298 117 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2520 3063 3170 4883 882 Size: 617 1021 543 107 1713 2219 Fragments arranged by size: 2219 1713 1021 617 543 107 BsaAI yAC'GTr Cuts at: 493 1204 4011 493 Size: 711 2807 2702 Fragments arranged by size: 2807 2702 711 BsaBI GATnn'nnATC Cuts at: 6214 6214 Size: 6220 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2987 3138 3375 4027 Size: 53 83 186 936 1454 151 237 652 Cuts at: 4027 4409 5044 275 Size: 382 635 1451 Fragments arranged by size: 1454 1451 936 652 635 382 237 186 151 83 53 BsaWI w'CCGG_w Cuts at: 3072 4653 5051 5325 5472 5883 3072 Size: 1581 398 274 147 411 3409 Fragments arranged by size: 3409 1581 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2566 2596 2639 2669 3293 Size: 30 165 30 1163 30 43 30 624 Cuts at: 3293 3323 1178 Size: 30 4075 Fragments arranged by size: 4075 1163 624 165 43 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 2300 2508 2687 1186 Size: 844 270 208 179 4719 Fragments arranged by size: 4719 844 270 208 179 BseYI C'CCAG_C Cuts at: 2831 3211 5423 5919 2831 Size: 380 2212 496 3132 Fragments arranged by size: 3132 2212 496 380 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2045 2177 3278 1163 Size: 573 309 132 1101 4105 Fragments arranged by size: 4105 1101 573 309 132 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2013 3670 4431 5459 1540 Size: 275 186 12 1657 761 1028 2301 Fragments arranged by size: 2301 1657 1028 761 275 186 12 BsiHKAI G_wGCw'C Cuts at: 4281 4366 5437 4281 Size: 85 1071 5064 Fragments arranged by size: 5064 1071 85 BsmI GAATG_Cn' Cuts at: 2818 3731 3824 2818 Size: 913 93 5214 Fragments arranged by size: 5214 913 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3366 3366 Size: 6220 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2227 2249 2652 3219 3539 4281 4366 Size: 399 437 22 403 567 320 742 85 Cuts at: 4366 5437 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 567 437 403 399 320 85 22 BspEI T'CCGG_A Cuts at: 5883 5883 Size: 6220 BspHI T'CATG_A Cuts at: 3363 4109 5839 3363 Size: 746 1730 3744 Fragments arranged by size: 3744 1730 746 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2332 2407 2445 3575 844 Size: 1488 75 38 1130 3489 Fragments arranged by size: 3489 1488 1130 75 38 BsrBI CCG'CTC Cuts at: 1394 1823 2109 4107 1394 Size: 429 286 1998 3507 Fragments arranged by size: 3507 1998 429 286 BsrDI GCAATG_nn' Cuts at: 65 1248 4715 4889 65 Size: 1183 3467 174 1396 Fragments arranged by size: 3467 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2141 2216 4864 5051 1700 Size: 110 331 75 2648 187 2869 Fragments arranged by size: 2869 2648 331 187 110 75 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6220 BssHII G'CGCG_C Cuts at: 1925 2178 1925 Size: 253 5967 BssSI C'ACGA_G Cuts at: 2477 2591 3206 4280 5292 2477 Size: 114 615 1074 1012 3405 Fragments arranged by size: 3405 1074 1012 615 114 BstAPI GCAn_nnn'nTGC Cuts at: 794 2164 2365 794 Size: 1370 201 4649 Fragments arranged by size: 4649 1370 201 BstBI TT'CG_AA Cuts at: 5024 5024 Size: 6220 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6220 BstXI CCAn_nnnn'nTGG Cuts at: 3004 3004 Size: 6220 BstYI r'GATC_y Cuts at: 1972 2028 2805 3550 3917 4302 4319 5771 5857 Size: 56 777 745 367 385 17 1452 86 Cuts at: 5857 5869 6215 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 777 745 385 367 346 86 56 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5668 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5481 Fragments arranged by size: 5481 683 56 BtsI GCAGTG_nn' Cuts at: 3736 4530 4550 3736 Size: 794 20 5406 Fragments arranged by size: 5406 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6220 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6185 DraI TTT'AAA Cuts at: 3524 3524 Size: 6220 DraIII CAC_nnn'GTG Cuts at: 2482 3406 2482 Size: 924 5296 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5227 809 Size: 507 3911 1802 Fragments arranged by size: 3911 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2700 3667 Size: 54 1425 50 275 18 180 690 967 Cuts at: 3667 4556 6185 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 967 889 690 275 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2010 3667 1537 Size: 275 198 1657 4090 Fragments arranged by size: 4090 1657 275 198 EarI CTCTTCn'nnn_ Cuts at: 1353 2774 2867 3109 3168 4150 1353 Size: 1421 93 242 59 982 3423 Fragments arranged by size: 3423 1421 982 242 93 59 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2021 4807 5181 5327 387 Size: 1064 138 432 2786 374 146 1280 Fragments arranged by size: 2786 1280 1064 432 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2417 2514 2567 2834 4283 4880 Size: 393 425 360 97 53 267 1449 597 Cuts at: 4880 5667 5935 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1449 787 597 425 393 360 268 267 97 53 EcoNI CCTnn'n_nnAGG Cuts at: 2132 2414 2441 2132 Size: 282 27 5911 Fragments arranged by size: 5911 282 27 EcoO109I rG'GnC_Cy Cuts at: 2125 2223 2618 3215 2125 Size: 98 395 597 5130 Fragments arranged by size: 5130 597 395 98 EcoRI G'AATT_C Cuts at: 3529 3907 3529 Size: 378 5842 FspI TGC'GCA Cuts at: 2817 4726 2817 Size: 1909 4311 HaeII r_GCGC'y Cuts at: 1678 2990 5367 1678 Size: 1312 2377 2531 Fragments arranged by size: 2531 2377 1312 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2566 2598 2639 2671 3293 Size: 32 135 32 994 32 41 32 622 Cuts at: 3293 3325 4861 4893 4935 4967 1373 Size: 32 1536 32 42 32 2626 Fragments arranged by size: 2626 1536 994 622 135 42 41 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3387 3564 3810 669 Size: 988 281 1449 177 246 3079 Fragments arranged by size: 3079 1449 988 281 246 177 HindIII A'AGCT_T Cuts at: 748 748 Size: 6220 HpaI GTT'AAC Cuts at: 3810 3810 Size: 6220 KasI G'GCGC_C Cuts at: 2986 2986 Size: 6220 KpnI G_GTAC'C Cuts at: 3545 3545 Size: 6220 MfeI C'AATT_G Cuts at: 3819 3819 Size: 6220 MluI A'CGCG_T Cuts at: 6096 6096 Size: 6220 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3132 5334 5518 1305 Size: 1827 2202 184 2007 Fragments arranged by size: 2202 2007 1827 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2702 10 Size: 54 1425 343 870 3528 Fragments arranged by size: 3528 1425 870 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4178 4537 4696 518 Size: 648 3012 359 159 2042 Fragments arranged by size: 3012 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2046 2164 2362 2698 2813 3636 4313 5461 Size: 440 118 198 336 115 823 677 1148 Cuts at: 5461 5706 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 823 677 440 336 245 198 118 115 NaeI GCC'GGC Cuts at: 2143 2218 2143 Size: 75 6145 NarI GG'CG_CC Cuts at: 2987 2987 Size: 6220 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5668 NdeI CA'TA_TG Cuts at: 387 387 Size: 6220 NgoMIV G'CCGG_C Cuts at: 2141 2216 2141 Size: 75 6145 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6220 NotI GC'GGCC_GC Cuts at: 3667 3667 Size: 6220 NspI r_CATG'y Cuts at: 1132 1229 2119 3115 3135 3250 3578 5123 1132 Size: 97 890 996 20 115 328 1545 2229 Fragments arranged by size: 2229 1545 996 890 328 115 97 20 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6220 PciI A'CATG_T Cuts at: 3111 3131 5119 3111 Size: 20 1988 4212 Fragments arranged by size: 4212 1988 20 PflMI CCAn_nnn'nTGG Cuts at: 1108 2509 2844 3211 3467 1108 Size: 1401 335 367 256 3861 Fragments arranged by size: 3861 1401 367 335 256 PfoI T'CCnGG_A Cuts at: 2754 2754 Size: 6220 PmeI GTTT'AAAC Cuts at: 3524 3524 Size: 6220 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2675 2707 4289 4321 5802 5834 2675 Size: 32 1582 32 1481 32 3061 Fragments arranged by size: 3061 1582 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2618 2618 Size: 6220 PshAI GACnn'nnGTC Cuts at: 1613 2585 1613 Size: 972 5248 PsiI TTA'TAA Cuts at: 3790 3790 Size: 6220 PspOMI G'GGCC_C Cuts at: 2223 2245 3215 2223 Size: 22 970 5228 Fragments arranged by size: 5228 970 22 PstI C_TGCA'G Cuts at: 830 2343 2361 2442 3572 830 Size: 1513 18 81 1130 3478 Fragments arranged by size: 3478 1513 1130 81 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6220 PvuII CAG'CTG Cuts at: 2164 2362 2164 Size: 198 6022 SalI G'TCGA_C Cuts at: 1936 3385 3562 1936 Size: 1449 177 4594 Fragments arranged by size: 4594 1449 177 SanDI GG'GwC_CC Cuts at: 2618 2618 Size: 6220 SbfI CC_TGCA'GG Cuts at: 2343 2442 3572 2343 Size: 99 1130 4991 Fragments arranged by size: 4991 1130 99 ScaI AGT'ACT Cuts at: 3095 4468 6180 3095 Size: 1373 1712 3135 Fragments arranged by size: 3135 1712 1373 SexAI A'CCwGG_T Cuts at: 2721 2721 Size: 6220 SfcI C'TryA_G Cuts at: 826 1045 2339 2357 2438 3568 4703 5384 5575 Size: 219 1294 18 81 1130 1135 681 191 Cuts at: 5575 826 Size: 1471 Fragments arranged by size: 1471 1294 1135 1130 681 219 191 81 18 SfoI GGC'GCC Cuts at: 2988 2988 Size: 6220 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 SgrAI Cr'CCGG_yG Cuts at: 1700 2216 1700 Size: 516 5704 SmaI CCC'GGG Cuts at: 1872 3547 1872 Size: 1675 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3296 3413 4324 5225 5487 820 Size: 197 926 1353 117 911 901 262 1553 Fragments arranged by size: 1553 1353 926 911 901 262 197 117 SnaBI TAC'GTA Cuts at: 493 4011 493 Size: 3518 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6220 SphI G_CATG'C Cuts at: 1132 2119 3578 1132 Size: 987 1459 3774 Fragments arranged by size: 3774 1459 987 SspI AAT'ATT Cuts at: 5 52 4144 5 Size: 47 4092 2081 Fragments arranged by size: 4092 2081 47 StuI AGG'CCT Cuts at: 1517 2447 1517 Size: 930 5290 StyI C'CwwG_G Cuts at: 513 1065 3659 513 Size: 552 2594 3074 Fragments arranged by size: 3074 2594 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4247 4264 4417 4602 5047 325 Size: 1255 2667 17 153 185 445 1498 Fragments arranged by size: 2667 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3093 3417 4466 Size: 274 80 33 51 1443 1116 324 1049 Cuts at: 4466 6178 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1116 1049 324 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6180 6212 6180 Size: 32 6188 Tth111I GACn'n_nGTC Cuts at: 1316 2723 1316 Size: 1407 4813 XbaI T'CTAG_A Cuts at: 3556 3556 Size: 6220 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3187 3187 Size: 6220 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6220 XmaI C'CCGG_G Cuts at: 1870 3545 1870 Size: 1675 4545 XmnI GAAnn'nnTTC Cuts at: 3447 4349 3447 Size: 902 5318 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3139 3376 4028 276 Size: 53 83 186 936 1605 237 652 2468 Fragments arranged by size: 2468 1605 936 652 237 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SfiI SrfI SwaI