Restriction map for
pFN21AB5915
(Linear) (Six-base) MAPSORT of: KIBB5915.seq Check: 802 from: 1 to: 1515
KIBB5915.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 402 1515
Size: 402 1113
AatII G_ACGT'C
Cuts at: 0 1136 1373 1515
Size: 1136 237 142
Fragments arranged by size:
1136 237 142
AccI GT'mk_AC
Cuts at: 0 1381 1515
Size: 1381 134
AclI AA'CG_TT
Cuts at: 0 938 1515
Size: 938 577
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 1515
Size: 562 953
AflIII A'CryG_T
Cuts at: 0 1106 1126 1301 1515
Size: 1106 20 175 214
Fragments arranged by size:
1106 214 175 20
AlwNI CAG_nnn'CTG
Cuts at: 0 159 360 552 1515
Size: 159 201 192 963
Fragments arranged by size:
963 201 192 159
ApaI G_GGCC'C
Cuts at: 0 222 244 1214 1515
Size: 222 22 970 301
Fragments arranged by size:
970 301 222 22
ApoI r'AATT_y
Cuts at: 0 972 1034 1515
Size: 972 62 481
Fragments arranged by size:
972 481 62
AvaI C'yCGr_G
Cuts at: 0 595 955 1515
Size: 595 360 560
Fragments arranged by size:
595 560 360
BanI G'GyrC_C
Cuts at: 0 981 1515
Size: 981 534
BanII G_rGCy'C
Cuts at: 0 222 244 647 1214 1515
Size: 222 22 403 567 301
Fragments arranged by size:
567 403 301 222 22
BbeI G_GCGC'C
Cuts at: 0 985 1515
Size: 985 530
BbvCI CC'TCA_GC
Cuts at: 0 169 553 1515
Size: 169 384 962
Fragments arranged by size:
962 384 169
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 946 980 1515
Size: 946 34 535
Fragments arranged by size:
946 535 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1045 1515
Size: 1045 470
BglI GCCn_nnn'nGGC
Cuts at: 0 217 1515
Size: 217 1298
BglII A'GATC_T
Cuts at: 0 800 1515
Size: 800 715
Bme1580I G_kGCm'C
Cuts at: 0 222 244 1214 1515
Size: 222 22 970 301
Fragments arranged by size:
970 301 222 22
BmgBI CAC'GTC
Cuts at: 0 344 1304 1515
Size: 344 960 211
Fragments arranged by size:
960 344 211
BmrI ACTGGGnnnn_n'
Cuts at: 0 486 581 1515
Size: 486 95 934
Fragments arranged by size:
934 486 95
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 342 1423 1515
Size: 342 1081 92
Fragments arranged by size:
1081 342 92
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 412 509 829 1515
Size: 52 360 97 320 686
Fragments arranged by size:
686 360 320 97 52
Bpu10I CC'TnA_GC
Cuts at: 0 124 169 553 658 1515
Size: 124 45 384 105 857
Fragments arranged by size:
857 384 124 105 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1276 1393 1515
Size: 1276 117 122
Fragments arranged by size:
1276 122 117
BsaI GGTCTCn'nnnn_
Cuts at: 0 515 1058 1165 1515
Size: 515 543 107 350
Fragments arranged by size:
543 515 350 107
BsaHI Gr'CG_yC
Cuts at: 0 982 1133 1370 1515
Size: 982 151 237 145
Fragments arranged by size:
982 237 151 145
BsaWI w'CCGG_w
Cuts at: 0 1067 1515
Size: 1067 448
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 561 591 634 664 1288 1318 1515
Size: 561 30 43 30 624 30 197
Fragments arranged by size:
624 561 197 43 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 295 503 682 1515
Size: 25 270 208 179 833
Fragments arranged by size:
833 270 208 179 25
BseYI C'CCAG_C
Cuts at: 0 826 1206 1515
Size: 826 380 309
Fragments arranged by size:
826 380 309
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 172 1273 1515
Size: 40 132 1101 242
Fragments arranged by size:
1101 242 132 40
BsiEI CG_ry'CG
Cuts at: 0 8 1515
Size: 8 1507
BsmI GAATG_Cn'
Cuts at: 0 813 1515
Size: 813 702
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1361 1515
Size: 1361 154
Bsp1286I G_dGCh'C
Cuts at: 0 222 244 647 1214 1515
Size: 222 22 403 567 301
Fragments arranged by size:
567 403 301 222 22
BspHI T'CATG_A
Cuts at: 0 1358 1515
Size: 1358 157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 327 402 440 1515
Size: 327 75 38 1075
Fragments arranged by size:
1075 327 75 38
BsrBI CCG'CTC
Cuts at: 0 104 1515
Size: 104 1411
BsrFI r'CCGG_y
Cuts at: 0 136 211 1515
Size: 136 75 1304
Fragments arranged by size:
1304 136 75
BssHII G'CGCG_C
Cuts at: 0 173 1515
Size: 173 1342
BssSI C'ACGA_G
Cuts at: 0 472 586 1201 1515
Size: 472 114 615 314
Fragments arranged by size:
615 472 314 114
BstAPI GCAn_nnn'nTGC
Cuts at: 0 159 360 1515
Size: 159 201 1155
Fragments arranged by size:
1155 201 159
BstXI CCAn_nnnn'nTGG
Cuts at: 0 999 1515
Size: 999 516
BstYI r'GATC_y
Cuts at: 0 23 800 1515
Size: 23 777 715
Fragments arranged by size:
777 715 23
DraIII CAC_nnn'GTG
Cuts at: 0 477 1401 1515
Size: 477 924 114
Fragments arranged by size:
924 477 114
EaeI y'GGCC_r
Cuts at: 0 5 695 1515
Size: 5 690 820
Fragments arranged by size:
820 690 5
EagI C'GGCC_G
Cuts at: 0 5 1515
Size: 5 1510
EarI CTCTTCn'nnn_
Cuts at: 0 769 862 1104 1163 1515
Size: 769 93 242 59 352
Fragments arranged by size:
769 352 242 93 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 16 1515
Size: 16 1499
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 412 509 562 829 1515
Size: 52 360 97 53 267 686
Fragments arranged by size:
686 360 267 97 53 52
EcoNI CCTnn'n_nnAGG
Cuts at: 0 127 409 436 1515
Size: 127 282 27 1079
Fragments arranged by size:
1079 282 127 27
EcoO109I rG'GnC_Cy
Cuts at: 0 120 218 613 1210 1515
Size: 120 98 395 597 305
Fragments arranged by size:
597 395 305 120 98
FspI TGC'GCA
Cuts at: 0 812 1515
Size: 812 703
HaeII r_GCGC'y
Cuts at: 0 985 1515
Size: 985 530
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 561 593 634 666 1288 1320 1515
Size: 561 32 41 32 622 32 195
Fragments arranged by size:
622 561 195 41 32 32 32
HincII GTy'rAC
Cuts at: 0 1382 1515
Size: 1382 133
KasI G'GCGC_C
Cuts at: 0 981 1515
Size: 981 534
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1127 1515
Size: 1127 388
MscI TGG'CCA
Cuts at: 0 697 1515
Size: 697 818
MspA1I CmG'CkG
Cuts at: 0 41 159 357 693 808 1515
Size: 41 118 198 336 115 707
Fragments arranged by size:
707 336 198 118 115 41
NaeI GCC'GGC
Cuts at: 0 138 213 1515
Size: 138 75 1302
Fragments arranged by size:
1302 138 75
NarI GG'CG_CC
Cuts at: 0 982 1515
Size: 982 533
NgoMIV G'CCGG_C
Cuts at: 0 136 211 1515
Size: 136 75 1304
Fragments arranged by size:
1304 136 75
NspI r_CATG'y
Cuts at: 0 114 1110 1130 1245 1515
Size: 114 996 20 115 270
Fragments arranged by size:
996 270 115 114 20
PciI A'CATG_T
Cuts at: 0 1106 1126 1515
Size: 1106 20 389
Fragments arranged by size:
1106 389 20
PflMI CCAn_nnn'nTGG
Cuts at: 0 504 839 1206 1462 1515
Size: 504 335 367 256 53
Fragments arranged by size:
504 367 335 256 53
PfoI T'CCnGG_A
Cuts at: 0 749 1515
Size: 749 766
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 670 702 1515
Size: 670 32 813
Fragments arranged by size:
813 670 32
PpuMI rG'GwC_Cy
Cuts at: 0 613 1515
Size: 613 902
PshAI GACnn'nnGTC
Cuts at: 0 580 1515
Size: 580 935
PspOMI G'GGCC_C
Cuts at: 0 218 240 1210 1515
Size: 218 22 970 305
Fragments arranged by size:
970 305 218 22
PstI C_TGCA'G
Cuts at: 0 338 356 437 1515
Size: 338 18 81 1078
Fragments arranged by size:
1078 338 81 18
PvuII CAG'CTG
Cuts at: 0 159 357 1515
Size: 159 198 1158
Fragments arranged by size:
1158 198 159
SalI G'TCGA_C
Cuts at: 0 1380 1515
Size: 1380 135
SanDI GG'GwC_CC
Cuts at: 0 613 1515
Size: 613 902
SbfI CC_TGCA'GG
Cuts at: 0 338 437 1515
Size: 338 99 1078
Fragments arranged by size:
1078 338 99
ScaI AGT'ACT
Cuts at: 0 1090 1515
Size: 1090 425
SexAI A'CCwGG_T
Cuts at: 0 716 1515
Size: 716 799
SfcI C'TryA_G
Cuts at: 0 334 352 433 1515
Size: 334 18 81 1082
Fragments arranged by size:
1082 334 81 18
SfoI GGC'GCC
Cuts at: 0 983 1515
Size: 983 532
SgrAI Cr'CCGG_yG
Cuts at: 0 211 1515
Size: 211 1304
SmlI C'TyrA_G
Cuts at: 0 1291 1408 1515
Size: 1291 117 107
Fragments arranged by size:
1291 117 107
SphI G_CATG'C
Cuts at: 0 114 1515
Size: 114 1401
StuI AGG'CCT
Cuts at: 0 442 1515
Size: 442 1073
TatI w'GTAC_w
Cuts at: 0 1088 1412 1515
Size: 1088 324 103
Fragments arranged by size:
1088 324 103
Tth111I GACn'n_nGTC
Cuts at: 0 718 1515
Size: 718 797
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1182 1515
Size: 1182 333
XmnI GAAnn'nnTTC
Cuts at: 0 1442 1515
Size: 1442 73
ZraI GAC'GTC
Cuts at: 0 1134 1371 1515
Size: 1134 237 144
Fragments arranged by size:
1134 237 144
Enzymes that do cut:
AarI AatII AccI AclI AcuI AflIII AlwNI ApaI
ApoI AvaI BanI BanII BbeI BbvCI BcgI BciVI
BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI
BssHII BssSI BstAPI BstXI BstYI DraIII EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I
HincII KasI MmeI MscI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PfoI PpiI PpuMI PshAI PspOMI
PstI PvuII SalI SanDI SbfI ScaI SexAI SfcI
SfoI SgrAI SmlI SphI StuI TatI Tth111I XcmI
XmnI ZraI
Enzymes that do not cut:
Acc65I AfeI AflII AgeI AhdI AleI AlfI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbsI
BclI BfrBI BlpI BmtI BsaAI BsaBI BsiHKAI BsiWI
BspEI BsrDI BsrGI BstBI BstEII BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EcoICRI EcoRI
EcoRV FalI FseI FspAI HindIII HpaI KpnI MfeI
MluI MslI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PmeI PmlI PsiI PspXI PsrI PvuI
RsrII SacI SacII SapI SfiI SgfI SmaI SnaBI
SpeI SrfI SspI StyI SwaI TaqII TstI XbaI
XhoI XmaI