(Circular) (Six-base) MAPSORT of: pFN21AB9123.seq Check: 8596 from: 1 to: 6220 pFN21AB9123 6220 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3394 3394 Size: 6220 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4030 278 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2425 3371 3563 1937 Size: 488 946 192 4594 Fragments arranged by size: 4594 946 488 192 Acc65I G'GTAC_C Cuts at: 3541 3541 Size: 6220 AclI AA'CG_TT Cuts at: 1563 4347 4720 1563 Size: 2784 373 3063 Fragments arranged by size: 3063 2784 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2115 3054 3381 3441 4283 5667 5935 1632 Size: 483 939 327 60 842 1384 268 1917 Fragments arranged by size: 1917 1384 939 842 483 327 268 60 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6023 AflIII A'CryG_T Cuts at: 1203 2644 5119 6096 1203 Size: 1441 2475 977 1327 Fragments arranged by size: 2475 1441 1327 977 AgeI A'CCGG_T Cuts at: 5051 5051 Size: 6220 AhdI GACnn_n'nnGTC Cuts at: 4949 4949 Size: 6220 AleI CACnn'nnGTG Cuts at: 1166 2051 1166 Size: 885 5335 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2166 1184 Size: 982 5238 AlwNI CAG_nnn'CTG Cuts at: 3963 5535 3963 Size: 1572 4648 ApaLI G'TGCA_C Cuts at: 4277 5433 4277 Size: 1156 5064 ApoI r'AATT_y Cuts at: 1493 2390 3529 3755 3907 6016 1493 Size: 897 1139 226 152 2109 1697 Fragments arranged by size: 2109 1697 1139 897 226 152 AseI AT'TA_AT Cuts at: 160 1032 3416 4774 160 Size: 872 2384 1358 1606 Fragments arranged by size: 2384 1606 1358 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 AvaI C'yCGr_G Cuts at: 1870 1943 3545 1870 Size: 73 1602 4545 Fragments arranged by size: 4545 1602 73 BamHI G'GATC_C Cuts at: 2656 2689 3550 3917 2656 Size: 33 861 367 4959 Fragments arranged by size: 4959 861 367 33 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3118 3541 4996 618 Size: 325 217 627 1331 423 1455 1842 Fragments arranged by size: 1842 1455 1331 627 423 325 217 BanII G_rGCy'C Cuts at: 1391 2012 2956 3539 1391 Size: 621 944 583 4072 Fragments arranged by size: 4072 944 621 583 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2121 3522 928 Size: 971 222 1401 3626 Fragments arranged by size: 3626 1401 971 222 BbvCI CC'TCA_GC Cuts at: 3061 3061 Size: 6220 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3263 3297 4411 4445 1903 Size: 34 1326 34 1114 34 3678 Fragments arranged by size: 3678 1326 1114 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3347 4112 5322 3347 Size: 765 1210 4245 Fragments arranged by size: 4245 1210 765 BclI T'GATC_A Cuts at: 1548 1548 Size: 6220 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4831 136 Size: 107 122 71 631 605 3159 1525 Fragments arranged by size: 3159 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2225 6215 2225 Size: 3990 2230 BlpI GC'TnA_GC Cuts at: 3255 3637 3255 Size: 382 5838 Bme1580I G_kGCm'C Cuts at: 1790 4281 5437 1790 Size: 2491 1156 2573 Fragments arranged by size: 2573 2491 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6220 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3951 4909 442 Size: 416 53 83 399 24 2534 958 1753 Fragments arranged by size: 2534 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6220 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2358 3030 4880 1239 Size: 1119 672 1850 2579 Fragments arranged by size: 2579 1850 1119 672 Bpu10I CC'TnA_GC Cuts at: 3061 3061 Size: 6220 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4345 5210 5508 4345 Size: 865 298 5057 Fragments arranged by size: 5057 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4883 882 Size: 617 3384 2219 Fragments arranged by size: 3384 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4011 493 Size: 711 2807 2702 Fragments arranged by size: 2807 2702 711 BsaBI GATnn'nnATC Cuts at: 6214 6214 Size: 6220 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4027 4409 5044 275 Size: 53 83 186 936 2494 382 635 1451 Fragments arranged by size: 2494 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4653 5051 5325 5472 5883 4653 Size: 398 274 147 411 4990 Fragments arranged by size: 4990 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2072 2102 2534 2564 1178 Size: 30 165 30 669 30 432 30 4834 Fragments arranged by size: 4834 669 432 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2859 2862 2865 2892 2895 2898 2922 3027 Size: 1673 3 3 27 3 3 24 105 Cuts at: 3027 3049 3138 1186 Size: 22 89 4268 Fragments arranged by size: 4268 1673 105 89 27 24 22 3 3 3 3 BseYI C'CCAG_C Cuts at: 5423 5919 5423 Size: 496 5724 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2162 2206 2949 3372 1163 Size: 573 426 44 743 423 4011 Fragments arranged by size: 4011 743 573 426 423 44 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3670 4431 5459 1540 Size: 275 186 1669 761 1028 2301 Fragments arranged by size: 2301 1669 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2012 2956 3256 4281 4366 5437 2012 Size: 944 300 1025 85 1071 2795 Fragments arranged by size: 2795 1071 1025 944 300 85 BsmI GAATG_Cn' Cuts at: 3731 3824 3731 Size: 93 6127 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2956 3256 3539 4281 4366 5437 Size: 399 222 944 300 283 742 85 1071 Cuts at: 5437 1391 Size: 2174 Fragments arranged by size: 2174 1071 944 742 399 300 283 222 85 BspEI T'CCGG_A Cuts at: 5883 5883 Size: 6220 BspHI T'CATG_A Cuts at: 4109 5839 4109 Size: 1730 4490 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3394 3575 844 Size: 2550 181 3489 Fragments arranged by size: 3489 2550 181 BsrBI CCG'CTC Cuts at: 1394 1823 4107 1394 Size: 429 2284 3507 Fragments arranged by size: 3507 2284 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4715 4889 65 Size: 1183 3467 174 1396 Fragments arranged by size: 3467 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4864 5051 1700 Size: 110 3054 187 2869 Fragments arranged by size: 3054 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6220 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6220 BssSI C'ACGA_G Cuts at: 4280 5292 4280 Size: 1012 5208 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6220 BstBI TT'CG_AA Cuts at: 2395 5024 2395 Size: 2629 3591 BstEII G'GTnAC_C Cuts at: 1184 2044 1184 Size: 860 5360 BstXI CCAn_nnnn'nTGG Cuts at: 2639 2639 Size: 6220 BstYI r'GATC_y Cuts at: 1972 2225 2452 2656 2689 3550 3917 4302 4319 Size: 253 227 204 33 861 367 385 17 Cuts at: 4319 5771 5857 5869 6215 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 861 385 367 346 253 227 204 86 33 17 12 BtgI C'CryG_G Cuts at: 513 1065 2036 2478 513 Size: 552 971 442 4255 Fragments arranged by size: 4255 971 552 442 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5481 Fragments arranged by size: 5481 683 56 BtsI GCAGTG_nn' Cuts at: 2142 2194 3736 4530 4550 2142 Size: 52 1542 794 20 3812 Fragments arranged by size: 3812 1542 794 52 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6220 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6185 DraI TTT'AAA Cuts at: 3524 3524 Size: 6220 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5227 809 Size: 507 3911 1802 Fragments arranged by size: 3911 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3284 3361 3667 Size: 54 1425 50 275 18 1454 77 306 Cuts at: 3667 4556 6185 8 Size: 889 1629 43 Fragments arranged by size: 1629 1454 1425 889 306 275 77 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3667 1537 Size: 275 1855 4090 Fragments arranged by size: 4090 1855 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2099 2585 2917 4150 1353 Size: 746 486 332 1233 3423 Fragments arranged by size: 3423 1233 746 486 332 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4807 5181 5327 387 Size: 1064 138 3218 374 146 1280 Fragments arranged by size: 3218 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2010 2954 2010 Size: 944 5276 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2115 2358 3030 3054 3381 3441 4283 Size: 393 483 243 672 24 327 60 842 Cuts at: 4283 4880 5667 5935 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 842 787 672 597 483 393 327 268 243 60 24 EcoO109I rG'GnC_Cy Cuts at: 2058 2897 3074 2058 Size: 839 177 5204 Fragments arranged by size: 5204 839 177 EcoRI G'AATT_C Cuts at: 3529 3907 3529 Size: 378 5842 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2375 3245 2375 Size: 870 5350 FspI TGC'GCA Cuts at: 4726 4726 Size: 6220 HaeII r_GCGC'y Cuts at: 1678 2931 5367 1678 Size: 1253 2436 2531 Fragments arranged by size: 2531 2436 1253 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2072 2104 3019 3051 3321 Size: 32 135 32 500 32 915 32 270 Cuts at: 3321 3353 4861 4893 4935 4967 1373 Size: 32 1508 32 42 32 2626 Fragments arranged by size: 2626 1508 915 500 270 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2426 2542 3564 3810 669 Size: 988 281 488 116 1022 246 3079 Fragments arranged by size: 3079 1022 988 488 281 246 116 HindIII A'AGCT_T Cuts at: 748 748 Size: 6220 HpaI GTT'AAC Cuts at: 3810 3810 Size: 6220 KpnI G_GTAC'C Cuts at: 3545 3545 Size: 6220 MfeI C'AATT_G Cuts at: 3819 3819 Size: 6220 MluI A'CGCG_T Cuts at: 6096 6096 Size: 6220 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3227 3364 5334 5518 1305 Size: 1922 137 1970 184 2007 Fragments arranged by size: 2007 1970 1922 184 137 MscI TGG'CCA Cuts at: 10 64 1489 1832 3363 10 Size: 54 1425 343 1531 2867 Fragments arranged by size: 2867 1531 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2051 4178 4537 4696 518 Size: 648 885 2127 359 159 2042 Fragments arranged by size: 2127 2042 885 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2038 2963 3636 4313 5461 5706 1606 Size: 432 925 673 677 1148 245 2120 Fragments arranged by size: 2120 1148 925 677 673 432 245 NcoI C'CATG_G Cuts at: 513 1065 2478 513 Size: 552 1413 4255 Fragments arranged by size: 4255 1413 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6220 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6220 NotI GC'GGCC_GC Cuts at: 3667 3667 Size: 6220 NspI r_CATG'y Cuts at: 1132 1229 2648 3578 5123 1132 Size: 97 1419 930 1545 2229 Fragments arranged by size: 2229 1545 1419 930 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6220 PciI A'CATG_T Cuts at: 2644 5119 2644 Size: 2475 3745 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6220 PmeI GTTT'AAAC Cuts at: 3524 3524 Size: 6220 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4289 4321 5802 5834 4289 Size: 32 1481 32 4675 Fragments arranged by size: 4675 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2058 2897 2058 Size: 839 5381 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6220 PsiI TTA'TAA Cuts at: 3790 3790 Size: 6220 PstI C_TGCA'G Cuts at: 830 3214 3572 830 Size: 2384 358 3478 Fragments arranged by size: 3478 2384 358 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6220 PvuII CAG'CTG Cuts at: 2963 2963 Size: 6220 SacI G_AGCT'C Cuts at: 2012 2956 2012 Size: 944 5276 SacII CC_GC'GG Cuts at: 2039 2039 Size: 6220 SalI G'TCGA_C Cuts at: 1936 2424 3562 1936 Size: 488 1138 4594 Fragments arranged by size: 4594 1138 488 SanDI GG'GwC_CC Cuts at: 2058 2058 Size: 6220 SbfI CC_TGCA'GG Cuts at: 3572 3572 Size: 6220 ScaI AGT'ACT Cuts at: 4468 6180 4468 Size: 1712 4508 SfcI C'TryA_G Cuts at: 826 1045 2484 3210 3568 4703 5384 5575 826 Size: 219 1439 726 358 1135 681 191 1471 Fragments arranged by size: 1471 1439 1135 726 681 358 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6220 SmaI CCC'GGG Cuts at: 1872 3547 1872 Size: 1675 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4324 5225 5487 820 Size: 197 926 2381 901 262 1553 Fragments arranged by size: 2381 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4011 493 Size: 3518 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6220 SphI G_CATG'C Cuts at: 1132 3578 1132 Size: 2446 3774 SspI AAT'ATT Cuts at: 5 52 2244 4144 5 Size: 47 2192 1900 2081 Fragments arranged by size: 2192 2081 1900 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6220 StyI C'CwwG_G Cuts at: 513 1065 2478 2601 3659 513 Size: 552 1413 123 1058 3074 Fragments arranged by size: 3074 1413 1058 552 123 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3398 3406 4247 4264 4417 4602 5047 Size: 1255 1818 8 841 17 153 185 445 Cuts at: 5047 325 Size: 1498 Fragments arranged by size: 1818 1498 1255 841 445 185 153 17 8 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4466 6178 96 Size: 274 80 33 51 1443 2489 1712 138 Fragments arranged by size: 2489 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6180 6212 6180 Size: 32 6188 Tth111I GACn'n_nGTC Cuts at: 1316 2125 1316 Size: 809 5411 XbaI T'CTAG_A Cuts at: 3556 3556 Size: 6220 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6220 XmaI C'CCGG_G Cuts at: 1870 3545 1870 Size: 1675 4545 XmnI GAAnn'nnTTC Cuts at: 2708 4349 2708 Size: 1641 4579 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4028 276 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SapI SexAI SfiI SfoI SrfI SwaI XcmI