(Circular) (Six-base) MAPSORT of: pFN21AB5045.seq Check: 6486 from: 1 to: 6664 pFN21AB5045 6664 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2984 3502 2984 Size: 518 6146 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4474 278 Size: 53 83 186 936 2938 2468 Fragments arranged by size: 2938 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2070 4007 1937 Size: 133 1937 4594 Fragments arranged by size: 4594 1937 133 Acc65I G'GTAC_C Cuts at: 2238 2874 3985 2238 Size: 636 1111 4917 Fragments arranged by size: 4917 1111 636 AclI AA'CG_TT Cuts at: 1563 4791 5164 1563 Size: 3228 373 3063 Fragments arranged by size: 3228 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4727 6111 6379 1632 Size: 3095 1384 268 1917 Fragments arranged by size: 3095 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 3119 820 Size: 197 2102 4365 Fragments arranged by size: 4365 2102 197 AflIII A'CryG_T Cuts at: 1203 5563 6540 1203 Size: 4360 977 1327 Fragments arranged by size: 4360 1327 977 AgeI A'CCGG_T Cuts at: 5495 5495 Size: 6664 AhdI GACnn_n'nnGTC Cuts at: 5393 5393 Size: 6664 AleI CACnn'nnGTG Cuts at: 1166 3263 1166 Size: 2097 4567 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6664 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2129 2161 2129 Size: 32 6632 AlwNI CAG_nnn'CTG Cuts at: 3260 4407 5979 3260 Size: 1147 1572 3945 Fragments arranged by size: 3945 1572 1147 ApaI G_GGCC'C Cuts at: 2133 3853 2133 Size: 1720 4944 ApaLI G'TGCA_C Cuts at: 2030 2185 4721 5877 2030 Size: 155 2536 1156 2817 Fragments arranged by size: 2817 2536 1156 155 ApoI r'AATT_y Cuts at: 1493 3973 4199 4351 6460 1493 Size: 2480 226 152 2109 1697 Fragments arranged by size: 2480 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5218 160 Size: 872 4186 1606 Fragments arranged by size: 4186 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6664 AvaI C'yCGr_G Cuts at: 1870 1943 3081 3989 1870 Size: 73 1138 908 4545 Fragments arranged by size: 4545 1138 908 73 BamHI G'GATC_C Cuts at: 3994 4361 3994 Size: 367 6297 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2238 2512 2874 3224 3663 Size: 325 217 627 451 274 362 350 439 Cuts at: 3663 3985 5440 618 Size: 322 1455 1842 Fragments arranged by size: 1842 1455 627 451 439 362 350 325 322 274 217 BanII G_rGCy'C Cuts at: 1391 2133 2460 2547 2732 3853 3983 1391 Size: 742 327 87 185 1121 130 4072 Fragments arranged by size: 4072 1121 742 327 185 130 87 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3581 3634 3729 928 Size: 971 1682 53 95 3863 Fragments arranged by size: 3863 1682 971 95 53 BbvCI CC'TCA_GC Cuts at: 2122 2870 2122 Size: 748 5916 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4855 4889 1903 Size: 34 2918 34 3678 Fragments arranged by size: 3678 2918 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4556 5766 4556 Size: 1210 5454 BclI T'GATC_A Cuts at: 1548 1548 Size: 6664 BfrBI ATG'CAT Cuts at: 3276 3276 Size: 6664 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5275 136 Size: 107 122 71 631 605 3603 1525 Fragments arranged by size: 3603 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3393 6659 3393 Size: 3266 3398 BlpI GC'TnA_GC Cuts at: 3179 3336 4081 3179 Size: 157 745 5762 Fragments arranged by size: 5762 745 157 Bme1580I G_kGCm'C Cuts at: 1790 2034 2133 2189 2517 3507 3853 4725 5881 Size: 244 99 56 328 990 346 872 1156 Cuts at: 5881 1790 Size: 2573 Fragments arranged by size: 2573 1156 990 872 346 328 244 99 56 BmgBI CAC'GTC Cuts at: 1318 3441 1318 Size: 2123 4541 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3270 4395 5353 Size: 416 53 83 399 24 1853 1125 958 Cuts at: 5353 442 Size: 1753 Fragments arranged by size: 1853 1753 1125 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2939 3623 1056 Size: 1883 684 4097 Fragments arranged by size: 4097 1883 684 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2581 2581 Size: 6664 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2327 2408 2576 2684 2693 5324 1239 Size: 1088 81 168 108 9 2631 2579 Fragments arranged by size: 2631 2579 1088 168 108 81 9 Bpu10I CC'TnA_GC Cuts at: 2122 2726 2870 2122 Size: 604 144 5916 Fragments arranged by size: 5916 604 144 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2531 2582 2720 4789 5654 5952 2531 Size: 51 138 2069 865 298 3243 Fragments arranged by size: 3243 2069 865 298 138 51 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3078 3377 5327 882 Size: 617 1579 299 1950 2219 Fragments arranged by size: 2219 1950 1579 617 299 BsaAI yAC'GTr Cuts at: 493 1204 3244 4455 493 Size: 711 2040 1211 2702 Fragments arranged by size: 2702 2040 1211 711 BsaBI GATnn'nnATC Cuts at: 6658 6658 Size: 6664 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4471 4853 5488 275 Size: 53 83 186 936 2938 382 635 1451 Fragments arranged by size: 2938 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3767 5097 5495 5769 5916 6327 3767 Size: 1330 398 274 147 411 4104 Fragments arranged by size: 4104 1330 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2129 2159 3026 3056 3745 Size: 30 165 30 726 30 867 30 689 Cuts at: 3745 3775 1178 Size: 30 4067 Fragments arranged by size: 4067 867 726 689 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2681 1186 Size: 1495 5169 BseYI C'CCAG_C Cuts at: 3730 5867 6363 3730 Size: 2137 496 4031 Fragments arranged by size: 4031 2137 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2121 2179 3869 1163 Size: 573 385 58 1690 3958 Fragments arranged by size: 3958 1690 573 385 58 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4114 4875 5903 1540 Size: 275 186 2113 761 1028 2301 Fragments arranged by size: 2301 2113 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2034 2189 2460 2547 2732 3056 3180 3332 3486 Size: 155 271 87 185 324 124 152 154 Cuts at: 3486 4725 4810 5881 2034 Size: 1239 85 1071 2817 Fragments arranged by size: 2817 1239 1071 324 271 185 155 154 152 124 87 85 BsmI GAATG_Cn' Cuts at: 2053 2788 4175 4268 2053 Size: 735 1387 93 4449 Fragments arranged by size: 4449 1387 735 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2133 2189 2460 2517 2547 2732 Size: 399 244 99 56 271 57 30 185 Cuts at: 2732 3056 3180 3332 3486 3507 3853 3983 4725 Size: 324 124 152 154 21 346 130 742 Cuts at: 4725 4810 5881 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 346 324 271 244 185 154 152 130 124 99 85 57 56 30 21 BspEI T'CCGG_A Cuts at: 6327 6327 Size: 6664 BspHI T'CATG_A Cuts at: 2751 3570 3808 4553 6283 2751 Size: 819 238 745 1730 3132 Fragments arranged by size: 3132 1730 819 745 238 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2011 2984 3502 4019 844 Size: 1167 973 518 517 3489 Fragments arranged by size: 3489 1167 973 518 517 BsrBI CCG'CTC Cuts at: 1394 1823 2779 4551 1394 Size: 429 956 1772 3507 Fragments arranged by size: 3507 1772 956 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5159 5333 65 Size: 1183 3911 174 1396 Fragments arranged by size: 3911 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3457 5308 5495 1700 Size: 110 1647 1851 187 2869 Fragments arranged by size: 2869 1851 1647 187 110 BsrGI T'GTAC_A Cuts at: 96 3267 96 Size: 3171 3493 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6664 BssSI C'ACGA_G Cuts at: 2155 3023 4724 5736 2155 Size: 868 1701 1012 3083 Fragments arranged by size: 3083 1701 1012 868 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6664 BstBI TT'CG_AA Cuts at: 5468 5468 Size: 6664 BstEII G'GTnAC_C Cuts at: 1184 3280 3449 1184 Size: 2096 169 4399 Fragments arranged by size: 4399 2096 169 BstXI CCAn_nnnn'nTGG Cuts at: 3514 3514 Size: 6664 BstYI r'GATC_y Cuts at: 1972 3204 3393 3994 4361 4746 4763 6215 6301 Size: 1232 189 601 367 385 17 1452 86 Cuts at: 6301 6313 6659 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1232 601 385 367 346 189 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3402 3402 Size: 6664 BtgI C'CryG_G Cuts at: 513 1065 2719 3387 3477 513 Size: 552 1654 668 90 3700 Fragments arranged by size: 3700 1654 668 552 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2092 487 Size: 683 56 866 5059 Fragments arranged by size: 5059 866 683 56 BtsI GCAGTG_nn' Cuts at: 3615 4180 4974 4994 3615 Size: 565 794 20 5285 Fragments arranged by size: 5285 794 565 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6664 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2888 2923 529 Size: 35 2324 35 4270 Fragments arranged by size: 4270 2324 35 35 DraI TTT'AAA Cuts at: 3968 3968 Size: 6664 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5671 809 Size: 507 4355 1802 Fragments arranged by size: 4355 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2370 2983 4111 Size: 54 1425 50 275 18 540 613 1128 Cuts at: 4111 5000 6629 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1128 889 613 540 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4111 1537 Size: 275 2299 4090 Fragments arranged by size: 4090 2299 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2845 4594 1353 Size: 1492 1749 3423 Fragments arranged by size: 3423 1749 1492 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2279 5251 5625 5771 387 Size: 1064 138 690 2972 374 146 1280 Fragments arranged by size: 2972 1280 1064 690 374 146 138 EcoICRI GAG'CTC Cuts at: 2458 2545 2730 2458 Size: 87 185 6392 Fragments arranged by size: 6392 185 87 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2327 2408 2576 2684 2693 4727 5324 Size: 393 695 81 168 108 9 2034 597 Cuts at: 5324 6111 6379 1239 Size: 787 268 1524 Fragments arranged by size: 2034 1524 787 695 597 393 268 168 108 81 9 EcoNI CCTnn'n_nnAGG Cuts at: 2204 3026 2204 Size: 822 5842 EcoO109I rG'GnC_Cy Cuts at: 2130 2197 2209 2311 2355 2427 3849 2130 Size: 67 12 102 44 72 1422 4945 Fragments arranged by size: 4945 1422 102 72 67 44 12 EcoRI G'AATT_C Cuts at: 3973 4351 3973 Size: 378 6286 FspI TGC'GCA Cuts at: 5170 5170 Size: 6664 HaeII r_GCGC'y Cuts at: 1678 5811 1678 Size: 4133 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3026 3058 5305 5337 5379 Size: 32 135 32 1454 32 2247 32 42 Cuts at: 5379 5411 1373 Size: 32 2626 Fragments arranged by size: 2626 2247 1454 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4008 4254 669 Size: 988 281 2070 246 3079 Fragments arranged by size: 3079 2070 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6664 HpaI GTT'AAC Cuts at: 4254 4254 Size: 6664 KpnI G_GTAC'C Cuts at: 2242 2878 3989 2242 Size: 636 1111 4917 Fragments arranged by size: 4917 1111 636 MfeI C'AATT_G Cuts at: 4263 4263 Size: 6664 MluI A'CGCG_T Cuts at: 6540 6540 Size: 6664 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3086 3107 3327 3375 5778 5962 1305 Size: 1781 21 220 48 2403 184 2007 Fragments arranged by size: 2403 2007 1781 220 184 48 21 MscI TGG'CCA Cuts at: 10 64 1489 1832 2372 10 Size: 54 1425 343 540 4302 Fragments arranged by size: 4302 1425 540 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2267 3005 3263 3275 4622 4981 5140 Size: 648 1101 738 258 12 1347 359 159 Cuts at: 5140 518 Size: 2042 Fragments arranged by size: 2042 1347 1101 738 648 359 258 159 12 MspA1I CmG'CkG Cuts at: 1606 2869 2988 3673 4080 4757 5905 6150 1606 Size: 1263 119 685 407 677 1148 245 2120 Fragments arranged by size: 2120 1263 1148 685 677 407 245 119 NaeI GCC'GGC Cuts at: 3459 3459 Size: 6664 NcoI C'CATG_G Cuts at: 513 1065 2719 513 Size: 552 1654 4458 Fragments arranged by size: 4458 1654 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6664 NgoMIV G'CCGG_C Cuts at: 3457 3457 Size: 6664 NheI G'CTAG_C Cuts at: 1052 2935 3619 1052 Size: 1883 684 4097 Fragments arranged by size: 4097 1883 684 NotI GC'GGCC_GC Cuts at: 4111 4111 Size: 6664 NsiI A_TGCA'T Cuts at: 3278 3278 Size: 6664 NspI r_CATG'y Cuts at: 1132 1229 2620 2638 3010 3276 4022 5567 1132 Size: 97 1391 18 372 266 746 1545 2229 Fragments arranged by size: 2229 1545 1391 746 372 266 97 18 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6664 PciI A'CATG_T Cuts at: 5563 5563 Size: 6664 PflMI CCAn_nnn'nTGG Cuts at: 1108 2305 3845 1108 Size: 1197 1540 3927 Fragments arranged by size: 3927 1540 1197 PmeI GTTT'AAAC Cuts at: 3968 3968 Size: 6664 PmlI CAC'GTG Cuts at: 3244 3244 Size: 6664 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2129 2161 2573 2605 4733 4765 6246 6278 2129 Size: 32 412 32 2128 32 1481 32 2515 Fragments arranged by size: 2515 2128 1481 412 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2209 2311 2209 Size: 102 6562 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6664 PsiI TTA'TAA Cuts at: 4234 4234 Size: 6664 PspOMI G'GGCC_C Cuts at: 2129 3849 2129 Size: 1720 4944 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2222 2254 2222 Size: 32 6632 PstI C_TGCA'G Cuts at: 830 2319 4016 830 Size: 1489 1697 3478 Fragments arranged by size: 3478 1697 1489 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6664 SacI G_AGCT'C Cuts at: 2460 2547 2732 2460 Size: 87 185 6392 Fragments arranged by size: 6392 185 87 SalI G'TCGA_C Cuts at: 1936 4006 1936 Size: 2070 4594 SbfI CC_TGCA'GG Cuts at: 4016 4016 Size: 6664 ScaI AGT'ACT Cuts at: 4912 6624 4912 Size: 1712 4952 SexAI A'CCwGG_T Cuts at: 2877 2877 Size: 6664 SfcI C'TryA_G Cuts at: 826 1045 2315 3355 4012 5147 5828 6019 826 Size: 219 1270 1040 657 1135 681 191 1471 Fragments arranged by size: 1471 1270 1135 1040 681 657 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6664 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6664 SmaI CCC'GGG Cuts at: 1872 3991 1872 Size: 2119 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2546 2597 2735 3081 3119 4768 Size: 197 926 603 51 138 346 38 1649 Cuts at: 4768 5669 5931 820 Size: 901 262 1553 Fragments arranged by size: 1649 1553 926 901 603 346 262 197 138 51 38 SnaBI TAC'GTA Cuts at: 493 4455 493 Size: 3962 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6664 SphI G_CATG'C Cuts at: 1132 4022 1132 Size: 2890 3774 SspI AAT'ATT Cuts at: 5 52 4588 5 Size: 47 4536 2081 Fragments arranged by size: 4536 2081 47 StuI AGG'CCT Cuts at: 1517 2707 3041 1517 Size: 1190 334 5140 Fragments arranged by size: 5140 1190 334 StyI C'CwwG_G Cuts at: 513 1065 2719 2906 3200 3936 4103 513 Size: 552 1654 187 294 736 167 3074 Fragments arranged by size: 3074 1654 736 552 294 187 167 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3213 4691 4708 4861 5046 5491 325 Size: 1255 1633 1478 17 153 185 445 1498 Fragments arranged by size: 1633 1498 1478 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3267 3420 3677 Size: 274 80 33 51 1443 1290 153 257 Cuts at: 3677 4910 6622 96 Size: 1233 1712 138 Fragments arranged by size: 1712 1443 1290 1233 274 257 153 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2083 2115 6624 6656 2083 Size: 32 4509 32 2091 Fragments arranged by size: 4509 2091 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6664 XbaI T'CTAG_A Cuts at: 4000 4000 Size: 6664 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3376 3376 Size: 6664 XhoI C'TCGA_G Cuts at: 1943 3081 1943 Size: 1138 5526 XmaI C'CCGG_G Cuts at: 1870 3989 1870 Size: 2119 4545 XmnI GAAnn'nnTTC Cuts at: 2690 4793 2690 Size: 2103 4561 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4472 276 Size: 53 83 186 936 2938 2468 Fragments arranged by size: 2938 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NruI PacI PfoI PspXI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI