Restriction map for
pFN21AB5034
##################################
# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BbeI BbrPI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BseAI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BsrDI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeIII MamI MfeI MluI MroI
MslI MunI MvnI NarI NdeI
NheI NotI NruI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TfiI Tsp45I
Tsp509I Tth111I Van91I XbaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 31 GTAGAC GT^[AC][GT]AC
ApaI 59 GGGCCC GGGCC^C
BamHI 410 GGATCC G^GATCC
BanI 823 GGTGCC G^G[CT][GA]CC
BbsI 639 GAAGAC GAAGAC(2/6)
BbvCI 870 GCTGAGG CCTCAGC(-5/-2)
BpuAI 639 GAAGAC GAAGAC(2/6)
BsaAI 400 TACGTG [CT]AC^GT[GA]
BsaI 739 GAGACC GGTCTC(1/5)
BsaWI 311 ACCGGA [AT]^CCGG[AT]
BsiEI 135 CGACCG CG[GA][CT]^CG
Bsp1407I 762 TGTACA T^GTACA
BspMI 369 GCAGGT ACCTGC(4/8)
BsrBI 406 GAGCGG CCGCTC(-3/-3)
BsrFI 718 GCCGGC [GA]^CCGG[CT]
BsrGI 762 TGTACA T^GTACA
BstXI 329 CCAAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 718 GCCGGC [GA]^CCGG[CT]
EcoT22I 455 ATGCAT ATGCA^T
FauI 464 CCCGC CCCGC(4/6)
HaeII 321 GGCGCT [GA]GCGC^[CT]
HphI 352 GGTGA GGTGA(8/7)
MaeII 401 ACGT A^CGT
NaeI 718 GCCGGC GCC^GGC
NcoI 299 CCATGG C^CATGG
NgoMIV 718 GCCGGC G^CCGGC
NsiI 455 ATGCAT ATGCA^T
PaeR7I 241 CTCGAG C^TCGAG
PpuMI 547 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 840 CAGCTG CAG^CTG
SacI 103 GAGCTC GAGCT^C
SmlI 241 CTCGAG C^T[CT][GA]AG
SspBI 762 TGTACA T^GTACA
TaqI 242 TCGA T^CGA
TthHB8I 242 TCGA T^CGA
XcmI 734 CCAGAGAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 241 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AlwNI 493 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
837 CAGCAGCTG
AspHI 103 GAGCTC G[AT]GC[AT]^C
518 GAGCAC
AvaI 241 CTCGAG C^[CT]CG[GA]G
483 CTCGGG
BalI 206 TGGCCA TGG^CCA
731 TGGCCA
BanII 59 GGGCCC G[GA]GC[CT]^C
103 GAGCTC
BcnI 138 CCGGG CC^[GC]GG
606 CCGGG
BfaI 64 CTAG C^TAG
916 CTAG
BglI 208 GCCACCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
667 GCCAACAAGGC
BpmI 886 CTCCAG CTGGAG(16/14)
829 CTGGAG
BsgI 307 CTGCAC GTGCAG(16/14)
373 GTGCAG
BsiHKAI 103 GAGCTC G[AT]GC[AT]^C
518 GAGCAC
BsmAI 739 GAGAC GTCTC(1/5)
779 GAGAC
BsoBI 241 CTCGAG C^[CT]CG[GA]G
483 CTCGGG
BstYI 410 GGATCC [GA]^GATC[CT]
477 GGATCT
CfoI 258 GCGC GCG^C
322 GCGC
DsaI 212 CCACGG C^C[GA][CT]GG
299 CCATGG
EcoNI 63 CCTAGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
240 CCTCGAGGAGG
HhaI 258 GCGC GCG^C
322 GCGC
HinP1I 258 GCGC G^CGC
322 GCGC
HinfI 37 GAGTC G^A[ACGT]TC
926 GACTC
MaeI 64 CTAG C^TAG
916 CTAG
MboII 501 GAAGA GAAGA(8/7)
639 GAAGA
MflI 410 GGATCC [GA]^GATC[CT]
477 GGATCT
MluNI 206 TGGCCA TGG^CCA
731 TGGCCA
MscI 206 TGGCCA TGG^CCA
731 TGGCCA
MseI 185 TTAA T^TAA
200 TTAA
NciI 138 CCGGG CC^[GC]GG
606 CCGGG
NspI 689 ACATGC [GA]CATG^[CT]
782 ACATGC
PleI 926 GACTC GAGTC(4/5)
37 GAGTC
Tru9I 185 TTAA T^TAA
200 TTAA
XhoII 410 GGATCC [GA]^GATC[CT]
477 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BmyI BsaJI BseRI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HpaII ItaI MboI MnlI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
StyI TseI TspRI