(Circular) (Six-base) MAPSORT of: pFN21AB5034.seq Check: 9122 from: 1 to: 5653 pFN21AB5034 5653 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2365 2365 Size: 5653 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3463 278 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2037 2996 1937 Size: 100 959 4594 Fragments arranged by size: 4594 959 100 Acc65I G'GTAC_C Cuts at: 2974 2974 Size: 5653 AclI AA'CG_TT Cuts at: 1563 3780 4153 1563 Size: 2217 373 3063 Fragments arranged by size: 3063 2217 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2135 2525 3716 5100 5368 1632 Size: 503 390 1191 1384 268 1917 Fragments arranged by size: 1917 1384 1191 503 390 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5456 AflIII A'CryG_T Cuts at: 1203 4552 5529 1203 Size: 3349 977 1327 Fragments arranged by size: 3349 1327 977 AgeI A'CCGG_T Cuts at: 4484 4484 Size: 5653 AhdI GACnn_n'nnGTC Cuts at: 4382 4382 Size: 5653 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5653 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5653 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2744 2776 2744 Size: 32 5621 AlwNI CAG_nnn'CTG Cuts at: 2503 2847 3396 4968 2503 Size: 344 549 1572 3188 Fragments arranged by size: 3188 1572 549 344 ApaI G_GGCC'C Cuts at: 2068 2068 Size: 5653 ApaLI G'TGCA_C Cuts at: 3710 4866 3710 Size: 1156 4497 ApoI r'AATT_y Cuts at: 1493 2962 3188 3340 5449 1493 Size: 1469 226 152 2109 1697 Fragments arranged by size: 2109 1697 1469 226 152 AseI AT'TA_AT Cuts at: 160 1032 4207 160 Size: 872 3175 1606 Fragments arranged by size: 3175 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 AvaI C'yCGr_G Cuts at: 1870 1943 2246 2488 2978 1870 Size: 73 303 242 490 4545 Fragments arranged by size: 4545 490 303 242 73 BamHI G'GATC_C Cuts at: 2415 2983 3350 2415 Size: 568 367 4718 Fragments arranged by size: 4718 568 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2828 2974 4429 618 Size: 325 217 627 1041 146 1455 1842 Fragments arranged by size: 1842 1455 1041 627 325 217 146 BanII G_rGCy'C Cuts at: 1391 2068 2112 2972 1391 Size: 677 44 860 4072 Fragments arranged by size: 4072 860 677 44 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2651 928 Size: 971 752 3930 Fragments arranged by size: 3930 971 752 BbvCI CC'TCA_GC Cuts at: 2876 2876 Size: 5653 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3844 3878 1903 Size: 34 1907 34 3678 Fragments arranged by size: 3678 1907 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3545 4755 3545 Size: 1210 4443 BclI T'GATC_A Cuts at: 1548 1548 Size: 5653 BfrBI ATG'CAT Cuts at: 2462 2462 Size: 5653 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2219 2678 4264 Size: 107 122 71 631 605 547 459 1586 Cuts at: 4264 136 Size: 1525 Fragments arranged by size: 1586 1525 631 605 547 459 122 107 71 BglII A'GATC_T Cuts at: 5648 5648 Size: 5653 BlpI GC'TnA_GC Cuts at: 3070 3070 Size: 5653 Bme1580I G_kGCm'C Cuts at: 1790 2068 2387 2833 3714 4870 1790 Size: 278 319 446 881 1156 2573 Fragments arranged by size: 2573 1156 881 446 319 278 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5653 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2152 2374 2551 Size: 416 53 83 399 24 735 222 177 Cuts at: 2551 3384 4342 442 Size: 833 958 1753 Fragments arranged by size: 1753 958 833 735 416 399 222 177 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5653 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2855 2876 4313 1239 Size: 1616 21 1437 2579 Fragments arranged by size: 2579 1616 1437 21 Bpu10I CC'TnA_GC Cuts at: 2876 2876 Size: 5653 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3778 4643 4941 3778 Size: 865 298 4490 Fragments arranged by size: 4490 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2738 4316 882 Size: 617 1239 1578 2219 Fragments arranged by size: 2219 1578 1239 617 BsaAI yAC'GTr Cuts at: 493 1204 2407 3444 493 Size: 711 1203 1037 2702 Fragments arranged by size: 2702 1203 1037 711 BsaBI GATnn'nnATC Cuts at: 5647 5647 Size: 5653 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3460 3842 4477 275 Size: 53 83 186 936 1927 382 635 1451 Fragments arranged by size: 1927 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2316 4086 4484 4758 4905 5316 2316 Size: 1770 398 274 147 411 2653 Fragments arranged by size: 2653 1770 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2517 2547 2744 2774 1178 Size: 30 165 30 1114 30 197 30 4057 Fragments arranged by size: 4057 1114 197 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2264 2384 2681 1186 Size: 1078 120 297 4158 Fragments arranged by size: 4158 1078 297 120 BseYI C'CCAG_C Cuts at: 2517 2537 2589 2885 4856 5352 2517 Size: 20 52 296 1971 496 2818 Fragments arranged by size: 2818 1971 496 296 52 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2297 2399 1163 Size: 573 561 102 4417 Fragments arranged by size: 4417 573 561 102 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2143 3103 3864 4892 1540 Size: 275 186 142 960 761 1028 2301 Fragments arranged by size: 2301 1028 960 761 275 186 142 BsiHKAI G_wGCw'C Cuts at: 2112 2527 3714 3799 4870 2112 Size: 415 1187 85 1071 2895 Fragments arranged by size: 2895 1187 1071 415 85 BsmI GAATG_Cn' Cuts at: 3164 3257 3164 Size: 93 5560 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2068 2112 2387 2527 2833 2972 3714 Size: 399 278 44 275 140 306 139 742 Cuts at: 3714 3799 4870 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 306 278 275 140 139 85 44 BspEI T'CCGG_A Cuts at: 5316 5316 Size: 5653 BspHI T'CATG_A Cuts at: 3542 5272 3542 Size: 1730 3923 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2365 3008 844 Size: 1521 643 3489 Fragments arranged by size: 3489 1521 643 BsrBI CCG'CTC Cuts at: 1394 1823 2413 3540 1394 Size: 429 590 1127 3507 Fragments arranged by size: 3507 1127 590 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4148 4322 65 Size: 1183 2900 174 1396 Fragments arranged by size: 2900 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2723 4297 4484 1700 Size: 110 913 1574 187 2869 Fragments arranged by size: 2869 1574 913 187 110 BsrGI T'GTAC_A Cuts at: 96 2767 96 Size: 2671 2982 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5653 BssSI C'ACGA_G Cuts at: 3713 4725 3713 Size: 1012 4641 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5653 BstBI TT'CG_AA Cuts at: 4457 4457 Size: 5653 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5653 BstXI CCAn_nnnn'nTGG Cuts at: 2341 2341 Size: 5653 BstYI r'GATC_y Cuts at: 1972 2415 2482 2983 3350 3735 3752 5204 5290 Size: 443 67 501 367 385 17 1452 86 Cuts at: 5290 5302 5648 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 501 443 385 367 346 86 67 17 12 BtgI C'CryG_G Cuts at: 513 1065 2217 2304 513 Size: 552 1152 87 3862 Fragments arranged by size: 3862 1152 552 87 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4914 Fragments arranged by size: 4914 683 56 BtsI GCAGTG_nn' Cuts at: 2387 3169 3963 3983 2387 Size: 782 794 20 4057 Fragments arranged by size: 4057 794 782 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5653 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5618 DraI TTT'AAA Cuts at: 2957 2957 Size: 5653 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4660 809 Size: 507 3344 1802 Fragments arranged by size: 3344 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2211 2736 2904 Size: 54 1425 50 275 18 381 525 168 Cuts at: 2904 3100 3989 5618 8 Size: 196 889 1629 43 Fragments arranged by size: 1629 1425 889 525 381 275 196 168 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3100 1537 Size: 275 1288 4090 Fragments arranged by size: 4090 1288 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3583 1353 Size: 2230 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4240 4614 4760 387 Size: 1064 138 2651 374 146 1280 Fragments arranged by size: 2651 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2110 2110 Size: 5653 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2135 2525 2855 2876 3716 4313 5100 Size: 393 503 390 330 21 840 597 787 Cuts at: 5100 5368 1239 Size: 268 1524 Fragments arranged by size: 1524 840 787 597 503 393 390 330 268 21 EcoNI CCTnn'n_nnAGG Cuts at: 2072 2249 2072 Size: 177 5476 EcoO109I rG'GnC_Cy Cuts at: 2064 2065 2553 2064 Size: 1 488 5164 Fragments arranged by size: 5164 488 1 EcoRI G'AATT_C Cuts at: 2962 3340 2962 Size: 378 5275 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2191 2191 Size: 5653 FspI TGC'GCA Cuts at: 4159 4159 Size: 5653 HaeII r_GCGC'y Cuts at: 1678 2330 4800 1678 Size: 652 2470 2531 Fragments arranged by size: 2531 2470 652 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4294 4326 4368 4400 1373 Size: 32 135 32 2722 32 42 32 2626 Fragments arranged by size: 2722 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2997 3243 669 Size: 988 281 1059 246 3079 Fragments arranged by size: 3079 1059 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5653 HpaI GTT'AAC Cuts at: 3243 3243 Size: 5653 KpnI G_GTAC'C Cuts at: 2978 2978 Size: 5653 MfeI C'AATT_G Cuts at: 3252 3252 Size: 5653 MluI A'CGCG_T Cuts at: 5529 5529 Size: 5653 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4767 4951 1305 Size: 3462 184 2007 Fragments arranged by size: 3462 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2213 2738 10 Size: 54 1425 343 381 525 2925 Fragments arranged by size: 2925 1425 525 381 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3611 3970 4129 518 Size: 648 2445 359 159 2042 Fragments arranged by size: 2445 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2012 2847 2909 3069 3746 4894 5139 1606 Size: 406 835 62 160 677 1148 245 2120 Fragments arranged by size: 2120 1148 835 677 406 245 160 62 NaeI GCC'GGC Cuts at: 2725 2725 Size: 5653 NcoI C'CATG_G Cuts at: 513 1065 2304 513 Size: 552 1239 3862 Fragments arranged by size: 3862 1239 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5653 NgoMIV G'CCGG_C Cuts at: 2723 2723 Size: 5653 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5653 NotI GC'GGCC_GC Cuts at: 3100 3100 Size: 5653 NsiI A_TGCA'T Cuts at: 2464 2464 Size: 5653 NspI r_CATG'y Cuts at: 1132 1229 2698 2791 3011 4556 1132 Size: 97 1469 93 220 1545 2229 Fragments arranged by size: 2229 1545 1469 220 97 93 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5653 PciI A'CATG_T Cuts at: 4552 4552 Size: 5653 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5653 PmeI GTTT'AAAC Cuts at: 2957 2957 Size: 5653 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2744 2776 3722 3754 5235 5267 2744 Size: 32 946 32 1481 32 3130 Fragments arranged by size: 3130 1481 946 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2553 2553 Size: 5653 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5653 PsiI TTA'TAA Cuts at: 3223 3223 Size: 5653 PspOMI G'GGCC_C Cuts at: 2064 2064 Size: 5653 PspXI vC'TCGA_Gb Cuts at: 2246 2246 Size: 5653 PstI C_TGCA'G Cuts at: 830 3005 830 Size: 2175 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5653 PvuII CAG'CTG Cuts at: 2847 2847 Size: 5653 SacI G_AGCT'C Cuts at: 2112 2112 Size: 5653 SalI G'TCGA_C Cuts at: 1936 2995 1936 Size: 1059 4594 SanDI GG'GwC_CC Cuts at: 2553 2553 Size: 5653 SbfI CC_TGCA'GG Cuts at: 3005 3005 Size: 5653 ScaI AGT'ACT Cuts at: 3901 5613 3901 Size: 1712 3941 SfcI C'TryA_G Cuts at: 826 1045 3001 4136 4817 5008 826 Size: 219 1956 1135 681 191 1471 Fragments arranged by size: 1956 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5653 SmaI CCC'GGG Cuts at: 1872 2980 1872 Size: 1108 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2246 3757 4658 4920 820 Size: 197 926 303 1511 901 262 1553 Fragments arranged by size: 1553 1511 926 901 303 262 197 SnaBI TAC'GTA Cuts at: 493 3444 493 Size: 2951 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5653 SphI G_CATG'C Cuts at: 1132 3011 1132 Size: 1879 3774 SspI AAT'ATT Cuts at: 5 52 3577 5 Size: 47 3525 2081 Fragments arranged by size: 3525 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5653 StyI C'CwwG_G Cuts at: 513 1065 2127 2304 2334 2388 2748 3092 513 Size: 552 1062 177 30 54 360 344 3074 Fragments arranged by size: 3074 1062 552 360 344 177 54 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2171 3680 3697 3850 4035 4480 325 Size: 1255 591 1509 17 153 185 445 1498 Fragments arranged by size: 1509 1498 1255 591 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2295 2767 3899 Size: 274 80 33 51 1443 318 472 1132 Cuts at: 3899 5611 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1132 472 318 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2517 2549 5613 5645 2517 Size: 32 3064 32 2525 Fragments arranged by size: 3064 2525 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5653 XbaI T'CTAG_A Cuts at: 2989 2989 Size: 5653 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2746 2746 Size: 5653 XhoI C'TCGA_G Cuts at: 1943 2246 1943 Size: 303 5350 XmaI C'CCGG_G Cuts at: 1870 2978 1870 Size: 1108 4545 XmnI GAAnn'nnTTC Cuts at: 3782 3782 Size: 5653 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3461 276 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI PacI PfoI PmlI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI