Restriction map for
pFN21AB5034
(Linear) (Six-base) MAPSORT of: KIBB5034.seq Check: 5669 from: 1 to: 948
KIBB5034.orf
With 182 enzymes: SgfI *
February 15, 2010 17:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 360 948
Size: 360 588
AccI GT'mk_AC
Cuts at: 0 32 948
Size: 32 916
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 520 948
Size: 130 390 428
Fragments arranged by size:
428 390 130
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 739 771 948
Size: 739 32 177
Fragments arranged by size:
739 177 32
AlwNI CAG_nnn'CTG
Cuts at: 0 498 842 948
Size: 498 344 106
Fragments arranged by size:
498 344 106
ApaI G_GGCC'C
Cuts at: 0 63 948
Size: 63 885
AvaI C'yCGr_G
Cuts at: 0 241 483 948
Size: 241 242 465
Fragments arranged by size:
465 242 241
BamHI G'GATC_C
Cuts at: 0 410 948
Size: 410 538
BanI G'GyrC_C
Cuts at: 0 823 948
Size: 823 125
BanII G_rGCy'C
Cuts at: 0 63 107 948
Size: 63 44 841
Fragments arranged by size:
841 63 44
BbsI GAAGACnn'nnnn_
Cuts at: 0 646 948
Size: 646 302
BbvCI CC'TCA_GC
Cuts at: 0 871 948
Size: 871 77
BfrBI ATG'CAT
Cuts at: 0 457 948
Size: 457 491
BglI GCCn_nnn'nGGC
Cuts at: 0 214 673 948
Size: 214 459 275
Fragments arranged by size:
459 275 214
Bme1580I G_kGCm'C
Cuts at: 0 63 382 828 948
Size: 63 319 446 120
Fragments arranged by size:
446 319 120 63
BmrI ACTGGGnnnn_n'
Cuts at: 0 147 369 546 948
Size: 147 222 177 402
Fragments arranged by size:
402 222 177 147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 850 871 948
Size: 850 21 77
Fragments arranged by size:
850 77 21
Bpu10I CC'TnA_GC
Cuts at: 0 871 948
Size: 871 77
BsaI GGTCTCn'nnnn_
Cuts at: 0 733 948
Size: 733 215
BsaAI yAC'GTr
Cuts at: 0 402 948
Size: 402 546
BsaWI w'CCGG_w
Cuts at: 0 311 948
Size: 311 637
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 512 542 739 769 948
Size: 512 30 197 30 179
Fragments arranged by size:
512 197 179 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 259 379 676 948
Size: 259 120 297 272
Fragments arranged by size:
297 272 259 120
BseYI C'CCAG_C
Cuts at: 0 512 532 584 880 948
Size: 512 20 52 296 68
Fragments arranged by size:
512 296 68 52 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 394 948
Size: 292 102 554
Fragments arranged by size:
554 292 102
BsiEI CG_ry'CG
Cuts at: 0 138 948
Size: 138 810
BsiHKAI G_wGCw'C
Cuts at: 0 107 522 948
Size: 107 415 426
Fragments arranged by size:
426 415 107
Bsp1286I G_dGCh'C
Cuts at: 0 63 107 382 522 828 948
Size: 63 44 275 140 306 120
Fragments arranged by size:
306 275 140 120 63 44
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 360 948
Size: 360 588
BsrBI CCG'CTC
Cuts at: 0 408 948
Size: 408 540
BsrFI r'CCGG_y
Cuts at: 0 718 948
Size: 718 230
BsrGI T'GTAC_A
Cuts at: 0 762 948
Size: 762 186
BstXI CCAn_nnnn'nTGG
Cuts at: 0 336 948
Size: 336 612
BstYI r'GATC_y
Cuts at: 0 410 477 948
Size: 410 67 471
Fragments arranged by size:
471 410 67
BtgI C'CryG_G
Cuts at: 0 212 299 948
Size: 212 87 649
Fragments arranged by size:
649 212 87
BtsI GCAGTG_nn'
Cuts at: 0 382 948
Size: 382 566
EaeI y'GGCC_r
Cuts at: 0 206 731 899 948
Size: 206 525 168 49
Fragments arranged by size:
525 206 168 49
EcoICRI GAG'CTC
Cuts at: 0 105 948
Size: 105 843
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 520 850 871 948
Size: 130 390 330 21 77
Fragments arranged by size:
390 330 130 77 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 67 244 948
Size: 67 177 704
Fragments arranged by size:
704 177 67
EcoO109I rG'GnC_Cy
Cuts at: 0 59 60 548 948
Size: 59 1 488 400
Fragments arranged by size:
488 400 59 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 186 948
Size: 186 762
HaeII r_GCGC'y
Cuts at: 0 325 948
Size: 325 623
MscI TGG'CCA
Cuts at: 0 208 733 948
Size: 208 525 215
Fragments arranged by size:
525 215 208
MspA1I CmG'CkG
Cuts at: 0 7 842 904 948
Size: 7 835 62 44
Fragments arranged by size:
835 62 44 7
NaeI GCC'GGC
Cuts at: 0 720 948
Size: 720 228
NcoI C'CATG_G
Cuts at: 0 299 948
Size: 299 649
NgoMIV G'CCGG_C
Cuts at: 0 718 948
Size: 718 230
NsiI A_TGCA'T
Cuts at: 0 459 948
Size: 459 489
NspI r_CATG'y
Cuts at: 0 693 786 948
Size: 693 93 162
Fragments arranged by size:
693 162 93
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 739 771 948
Size: 739 32 177
Fragments arranged by size:
739 177 32
PpuMI rG'GwC_Cy
Cuts at: 0 548 948
Size: 548 400
PspOMI G'GGCC_C
Cuts at: 0 59 948
Size: 59 889
PspXI vC'TCGA_Gb
Cuts at: 0 241 948
Size: 241 707
PvuII CAG'CTG
Cuts at: 0 842 948
Size: 842 106
SacI G_AGCT'C
Cuts at: 0 107 948
Size: 107 841
SanDI GG'GwC_CC
Cuts at: 0 548 948
Size: 548 400
SmlI C'TyrA_G
Cuts at: 0 241 948
Size: 241 707
StyI C'CwwG_G
Cuts at: 0 122 299 329 383 743 948
Size: 122 177 30 54 360 205
Fragments arranged by size:
360 205 177 122 54 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 166 948
Size: 166 782
TatI w'GTAC_w
Cuts at: 0 290 762 948
Size: 290 472 186
Fragments arranged by size:
472 290 186
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 512 544 948
Size: 512 32 404
Fragments arranged by size:
512 404 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 741 948
Size: 741 207
XhoI C'TCGA_G
Cuts at: 0 241 948
Size: 241 707
Enzymes that do cut:
AarI AccI AcuI AloI AlwNI ApaI AvaI BamHI
BanI BanII BbsI BbvCI BfrBI BglI Bme1580I BmrI
BpmI Bpu10I BsaI BsaAI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BsrGI
BstXI BstYI BtgI BtsI EaeI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII MscI MspA1I NaeI NcoI NgoMIV
NsiI NspI PpiI PpuMI PspOMI PspXI PvuII SacI
SanDI SmlI StyI TaqII TatI TstI XcmI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BcgI BciVI BclI BglII BlpI BmgBI
BmtI BplI BpuEI BsaBI BsaHI BsiWI BsmI BsmBI
BspEI BspHI BsrDI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoRI EcoRV FseI FspI FspAI
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MslI NarI NdeI NheI NotI NruI PacI
PasI PciI PflMI PfoI PmeI PmlI PshAI PsiI
PsrI PstI PvuI RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XmaI XmnI ZraI