(Circular) (Six-base) MAPSORT of: pFN21AB3324.seq Check: 2010 from: 1 to: 6520 pFN21AB3324 6520 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4330 278 Size: 53 83 186 936 2794 2468 Fragments arranged by size: 2794 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3300 3646 3863 1937 Size: 1363 346 217 4594 Fragments arranged by size: 4594 1363 346 217 Acc65I G'GTAC_C Cuts at: 2649 3841 2649 Size: 1192 5328 AclI AA'CG_TT Cuts at: 1563 4647 5020 1563 Size: 3084 373 3063 Fragments arranged by size: 3084 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4583 5967 6235 1632 Size: 2951 1384 268 1917 Fragments arranged by size: 2951 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6323 AflIII A'CryG_T Cuts at: 1203 3135 5419 6396 1203 Size: 1932 2284 977 1327 Fragments arranged by size: 2284 1932 1327 977 AgeI A'CCGG_T Cuts at: 5351 5351 Size: 6520 AhdI GACnn_n'nnGTC Cuts at: 3642 5249 3642 Size: 1607 4913 AleI CACnn'nnGTG Cuts at: 1166 3150 1166 Size: 1984 4536 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6520 AlwNI CAG_nnn'CTG Cuts at: 2138 2262 4263 5835 2138 Size: 124 2001 1572 2823 Fragments arranged by size: 2823 2001 1572 124 ApaLI G'TGCA_C Cuts at: 4577 5733 4577 Size: 1156 5364 ApoI r'AATT_y Cuts at: 1493 2161 2224 2456 2889 3015 3374 3698 3829 Size: 668 63 232 433 126 359 324 131 Cuts at: 3829 4055 4207 6316 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 668 433 359 324 232 226 152 131 126 63 AseI AT'TA_AT Cuts at: 160 1032 5074 160 Size: 872 4042 1606 Fragments arranged by size: 4042 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6520 AvaI C'yCGr_G Cuts at: 1870 1943 2562 3845 1870 Size: 73 619 1283 4545 Fragments arranged by size: 4545 1283 619 73 AvrII C'CTAG_G Cuts at: 2201 2201 Size: 6520 BamHI G'GATC_C Cuts at: 3850 4217 3850 Size: 367 6153 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2649 3841 5296 618 Size: 325 217 627 862 1192 1455 1842 Fragments arranged by size: 1842 1455 1192 862 627 325 217 BanII G_rGCy'C Cuts at: 1391 3839 1391 Size: 2448 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2184 2312 2878 928 Size: 971 285 128 566 4570 Fragments arranged by size: 4570 971 566 285 128 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4711 4745 1903 Size: 34 2774 34 3678 Fragments arranged by size: 3678 2774 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4412 5622 4412 Size: 1210 5310 BclI T'GATC_A Cuts at: 1548 2941 3069 1548 Size: 1393 128 4999 Fragments arranged by size: 4999 1393 128 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5131 136 Size: 107 122 71 631 605 3459 1525 Fragments arranged by size: 3459 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2982 6515 2982 Size: 3533 2987 BlpI GC'TnA_GC Cuts at: 3937 3937 Size: 6520 Bme1580I G_kGCm'C Cuts at: 1790 2367 2505 4581 5737 1790 Size: 577 138 2076 1156 2573 Fragments arranged by size: 2573 2076 1156 577 138 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6520 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4251 5209 442 Size: 416 53 83 399 24 2834 958 1753 Fragments arranged by size: 2834 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6520 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2071 3035 3799 5180 1239 Size: 832 964 764 1381 2579 Fragments arranged by size: 2579 1381 964 832 764 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2027 4645 5510 5808 2027 Size: 2618 865 298 2739 Fragments arranged by size: 2739 2618 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2073 5183 882 Size: 617 574 3110 2219 Fragments arranged by size: 3110 2219 617 574 BsaAI yAC'GTr Cuts at: 493 1204 4311 493 Size: 711 3107 2702 Fragments arranged by size: 3107 2702 711 BsaBI GATnn'nnATC Cuts at: 6514 6514 Size: 6520 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4327 4709 5344 275 Size: 53 83 186 936 2794 382 635 1451 Fragments arranged by size: 2794 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3258 3668 4953 5351 5625 5772 6183 3258 Size: 410 1285 398 274 147 411 3595 Fragments arranged by size: 3595 1285 411 410 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6295 Fragments arranged by size: 6295 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2781 1186 Size: 1595 4925 BseYI C'CCAG_C Cuts at: 2077 5723 6219 2077 Size: 3646 496 2378 Fragments arranged by size: 3646 2378 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3149 1163 Size: 573 1413 4534 Fragments arranged by size: 4534 1413 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2016 3970 4731 5759 1540 Size: 275 186 15 1954 761 1028 2301 Fragments arranged by size: 2301 1954 1028 761 275 186 15 BsiHKAI G_wGCw'C Cuts at: 2057 2815 3628 4581 4666 5737 2057 Size: 758 813 953 85 1071 2840 Fragments arranged by size: 2840 1071 953 813 758 85 BsmI GAATG_Cn' Cuts at: 2791 3011 3664 4031 4124 2791 Size: 220 653 367 93 5187 Fragments arranged by size: 5187 653 367 220 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2367 2505 2815 3628 3839 4581 Size: 399 267 310 138 310 813 211 742 Cuts at: 4581 4666 5737 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 813 742 399 310 310 267 211 138 85 BspEI T'CCGG_A Cuts at: 3258 3668 6183 3258 Size: 410 2515 3595 Fragments arranged by size: 3595 2515 410 BspHI T'CATG_A Cuts at: 4409 6139 4409 Size: 1730 4790 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3206 3875 844 Size: 2362 669 3489 Fragments arranged by size: 3489 2362 669 BsrBI CCG'CTC Cuts at: 1394 1823 4407 1394 Size: 429 2584 3507 Fragments arranged by size: 3507 2584 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3216 3408 5015 5189 65 Size: 1183 1968 192 1607 174 1396 Fragments arranged by size: 1968 1607 1396 1183 192 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5164 5351 1700 Size: 110 3354 187 2869 Fragments arranged by size: 3354 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6520 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6520 BssSI C'ACGA_G Cuts at: 4580 5592 4580 Size: 1012 5508 BstAPI GCAn_nnn'nTGC Cuts at: 794 2297 794 Size: 1503 5017 BstBI TT'CG_AA Cuts at: 5324 5324 Size: 6520 BstEII G'GTnAC_C Cuts at: 1184 3193 1184 Size: 2009 4511 BstYI r'GATC_y Cuts at: 1972 2982 3598 3850 4217 4602 4619 6071 6157 Size: 1010 616 252 367 385 17 1452 86 Cuts at: 6157 6169 6515 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1010 616 385 367 346 252 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3093 513 Size: 552 939 1089 3940 Fragments arranged by size: 3940 1089 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2131 487 Size: 683 56 905 4876 Fragments arranged by size: 4876 905 683 56 BtsI GCAGTG_nn' Cuts at: 2295 2438 2893 4036 4830 4850 2295 Size: 143 455 1143 794 20 3965 Fragments arranged by size: 3965 1143 794 455 143 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6520 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6485 DraI TTT'AAA Cuts at: 3824 3824 Size: 6520 DraIII CAC_nnn'GTG Cuts at: 2621 2621 Size: 6520 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5527 809 Size: 507 4211 1802 Fragments arranged by size: 4211 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2322 3967 Size: 54 1425 50 275 18 183 309 1645 Cuts at: 3967 4856 6485 8 Size: 889 1629 43 Fragments arranged by size: 1645 1629 1425 889 309 275 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2013 3967 1537 Size: 275 201 1954 4090 Fragments arranged by size: 4090 1954 275 201 EarI CTCTTCn'nnn_ Cuts at: 1353 2734 3667 4450 1353 Size: 1381 933 783 3423 Fragments arranged by size: 3423 1381 933 783 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2111 5107 5481 5627 387 Size: 1064 138 522 2996 374 146 1280 Fragments arranged by size: 2996 1280 1064 522 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2071 3035 3799 4583 5180 5967 6235 Size: 393 439 964 764 784 597 787 268 Cuts at: 6235 1239 Size: 1524 Fragments arranged by size: 1524 964 787 784 764 597 439 393 268 EcoO109I rG'GnC_Cy Cuts at: 3384 3570 3384 Size: 186 6334 EcoRI G'AATT_C Cuts at: 2161 2224 2889 3015 3698 3829 4207 2161 Size: 63 665 126 683 131 378 4474 Fragments arranged by size: 4474 683 665 378 131 126 63 FspI TGC'GCA Cuts at: 5026 5026 Size: 6520 HaeII r_GCGC'y Cuts at: 1678 5667 1678 Size: 3989 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2420 2452 2597 2629 2698 Size: 32 135 32 848 32 145 32 69 Cuts at: 2698 2730 3221 3253 3273 3305 3628 3660 5161 Size: 32 491 32 20 32 323 32 1501 Cuts at: 5161 5193 5235 5267 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1501 848 491 323 145 135 69 42 32 32 32 32 32 32 32 32 32 32 20 HincII GTy'rAC Cuts at: 669 1657 1938 3864 4110 669 Size: 988 281 1926 246 3079 Fragments arranged by size: 3079 1926 988 281 246 HindIII A'AGCT_T Cuts at: 748 2684 748 Size: 1936 4584 HpaI GTT'AAC Cuts at: 4110 4110 Size: 6520 KpnI G_GTAC'C Cuts at: 2653 3845 2653 Size: 1192 5328 MfeI C'AATT_G Cuts at: 4119 4119 Size: 6520 MluI A'CGCG_T Cuts at: 6396 6396 Size: 6520 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3253 5634 5818 1305 Size: 1948 2381 184 2007 Fragments arranged by size: 2381 2007 1948 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2324 10 Size: 54 1425 343 492 4206 Fragments arranged by size: 4206 1425 492 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2300 3004 3150 3191 4478 4837 4996 Size: 648 1134 704 146 41 1287 359 159 Cuts at: 4996 518 Size: 2042 Fragments arranged by size: 2042 1287 1134 704 648 359 159 146 41 MspA1I CmG'CkG Cuts at: 1606 2250 3764 3936 4613 5761 6006 1606 Size: 644 1514 172 677 1148 245 2120 Fragments arranged by size: 2120 1514 1148 677 644 245 172 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5029 Fragments arranged by size: 5029 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6520 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6520 NotI GC'GGCC_GC Cuts at: 3967 3967 Size: 6520 NspI r_CATG'y Cuts at: 1132 1229 2107 2674 3498 3878 5423 1132 Size: 97 878 567 824 380 1545 2229 Fragments arranged by size: 2229 1545 878 824 567 380 97 PasI CC'CwG_GG Cuts at: 1348 2132 1348 Size: 784 5736 PciI A'CATG_T Cuts at: 5419 5419 Size: 6520 PflMI CCAn_nnn'nTGG Cuts at: 1108 2138 1108 Size: 1030 5490 PmeI GTTT'AAAC Cuts at: 3824 3824 Size: 6520 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4589 4621 6102 6134 4589 Size: 32 1481 32 4975 Fragments arranged by size: 4975 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3384 3384 Size: 6520 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6520 PsiI TTA'TAA Cuts at: 4090 4090 Size: 6520 PstI C_TGCA'G Cuts at: 830 3634 3872 830 Size: 2804 238 3478 Fragments arranged by size: 3478 2804 238 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6520 PvuII CAG'CTG Cuts at: 2250 3764 2250 Size: 1514 5006 SalI G'TCGA_C Cuts at: 1936 3862 1936 Size: 1926 4594 SbfI CC_TGCA'GG Cuts at: 3634 3872 3634 Size: 238 6282 ScaI AGT'ACT Cuts at: 3367 4768 6480 3367 Size: 1401 1712 3407 Fragments arranged by size: 3407 1712 1401 SfcI C'TryA_G Cuts at: 826 1045 3630 3686 3868 5003 5684 5875 826 Size: 219 2585 56 182 1135 681 191 1471 Fragments arranged by size: 2585 1471 1135 681 219 191 182 56 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6520 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6520 SmaI CCC'GGG Cuts at: 1872 3847 1872 Size: 1975 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2042 4624 5525 5787 820 Size: 197 926 99 2582 901 262 1553 Fragments arranged by size: 2582 1553 926 901 262 197 99 SnaBI TAC'GTA Cuts at: 493 4311 493 Size: 3818 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6520 SphI G_CATG'C Cuts at: 1132 2107 2674 3878 1132 Size: 975 567 1204 3774 Fragments arranged by size: 3774 1204 975 567 SspI AAT'ATT Cuts at: 5 52 4444 5 Size: 47 4392 2081 Fragments arranged by size: 4392 2081 47 StuI AGG'CCT Cuts at: 1517 2727 1517 Size: 1210 5310 StyI C'CwwG_G Cuts at: 513 1065 2004 2201 3959 513 Size: 552 939 197 1758 3074 Fragments arranged by size: 3074 1758 939 552 197 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4547 4564 4717 4902 5347 325 Size: 1255 2967 17 153 185 445 1498 Fragments arranged by size: 2967 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3315 3365 3423 Size: 274 80 33 51 1443 1338 50 58 Cuts at: 3423 4766 6478 96 Size: 1343 1712 138 Fragments arranged by size: 1712 1443 1343 1338 274 138 80 58 51 50 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3550 3582 6480 6512 3550 Size: 32 2898 32 3558 Fragments arranged by size: 3558 2898 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6520 XbaI T'CTAG_A Cuts at: 3856 3856 Size: 6520 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6520 XmaI C'CCGG_G Cuts at: 1870 3845 1870 Size: 1975 4545 XmnI GAAnn'nnTTC Cuts at: 3159 4649 3159 Size: 1490 5030 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4328 276 Size: 53 83 186 936 2794 2468 Fragments arranged by size: 2794 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI