(Circular) (Six-base) MAPSORT of: pFN21AE1073.seq Check: 4606 from: 1 to: 7138 pFN21AE1073 7138 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4948 278 Size: 53 83 186 936 3412 2468 Fragments arranged by size: 3412 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2070 2116 2325 4481 1937 Size: 133 46 209 2156 4594 Fragments arranged by size: 4594 2156 209 133 46 Acc65I G'GTAC_C Cuts at: 4459 4459 Size: 7138 AclI AA'CG_TT Cuts at: 1563 5265 5638 1563 Size: 3702 373 3063 Fragments arranged by size: 3702 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2292 2555 2586 2774 2871 4265 4340 5201 Size: 660 263 31 188 97 1394 75 861 Cuts at: 5201 6585 6853 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1394 1384 861 660 268 263 188 97 75 31 AflII C'TTAA_G Cuts at: 820 1017 3779 820 Size: 197 2762 4179 Fragments arranged by size: 4179 2762 197 AflIII A'CryG_T Cuts at: 1203 6037 7014 1203 Size: 4834 977 1327 Fragments arranged by size: 4834 1327 977 AgeI A'CCGG_T Cuts at: 5969 5969 Size: 7138 AhdI GACnn_n'nnGTC Cuts at: 5867 5867 Size: 7138 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7138 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2148 1184 Size: 964 6174 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3054 3086 3054 Size: 32 7106 AlwNI CAG_nnn'CTG Cuts at: 3824 4881 6453 3824 Size: 1057 1572 4509 Fragments arranged by size: 4509 1572 1057 ApaLI G'TGCA_C Cuts at: 3417 5195 6351 3417 Size: 1778 1156 4204 Fragments arranged by size: 4204 1778 1156 ApoI r'AATT_y Cuts at: 1493 2292 3702 4447 4673 4825 6934 1493 Size: 799 1410 745 226 152 2109 1697 Fragments arranged by size: 2109 1697 1410 799 745 226 152 AseI AT'TA_AT Cuts at: 160 1032 5692 160 Size: 872 4660 1606 Fragments arranged by size: 4660 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7138 AvaI C'yCGr_G Cuts at: 1870 1943 3693 4463 1870 Size: 73 1750 770 4545 Fragments arranged by size: 4545 1750 770 73 BamHI G'GATC_C Cuts at: 4121 4468 4835 4121 Size: 347 367 6424 Fragments arranged by size: 6424 367 347 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4459 5914 618 Size: 325 217 627 2672 1455 1842 Fragments arranged by size: 2672 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2085 3072 4299 4457 1391 Size: 694 987 1227 158 4072 Fragments arranged by size: 4072 1227 987 694 158 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6167 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5329 5363 1903 Size: 34 50 34 3308 34 3678 Fragments arranged by size: 3678 3308 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5030 6240 5030 Size: 1210 5928 BclI T'GATC_A Cuts at: 1548 2185 1548 Size: 637 6501 BfrBI ATG'CAT Cuts at: 3956 3956 Size: 7138 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5749 136 Size: 107 122 71 631 605 4077 1525 Fragments arranged by size: 4077 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7133 7133 Size: 7138 BlpI GC'TnA_GC Cuts at: 3795 4555 3795 Size: 760 6378 Bme1580I G_kGCm'C Cuts at: 1790 3421 3694 5199 6355 1790 Size: 1631 273 1505 1156 2573 Fragments arranged by size: 2573 1631 1505 1156 273 BmgBI CAC'GTC Cuts at: 1318 2329 1318 Size: 1011 6127 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4869 5827 442 Size: 416 53 83 399 24 3452 958 1753 Fragments arranged by size: 3452 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3491 1056 Size: 2435 4703 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3056 4419 5798 1239 Size: 1817 1363 1379 2579 Fragments arranged by size: 2579 1817 1379 1363 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3821 4127 5263 6128 6426 3821 Size: 306 1136 865 298 4533 Fragments arranged by size: 4533 1136 865 306 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5801 882 Size: 617 4302 2219 Fragments arranged by size: 4302 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4411 4929 493 Size: 711 3207 518 2702 Fragments arranged by size: 3207 2702 711 518 BsaBI GATnn'nnATC Cuts at: 7132 7132 Size: 7138 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4945 5327 5962 275 Size: 53 83 186 936 3412 382 635 1451 Fragments arranged by size: 3412 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2300 5571 5969 6243 6390 6801 2300 Size: 3271 398 274 147 411 2637 Fragments arranged by size: 3271 2637 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2313 2343 3054 3084 1178 Size: 30 165 30 910 30 711 30 5232 Fragments arranged by size: 5232 910 711 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3659 1186 Size: 2473 4665 BseYI C'CCAG_C Cuts at: 6341 6837 6341 Size: 496 6642 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3154 4223 1163 Size: 573 1418 1069 4078 Fragments arranged by size: 4078 1418 1069 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2098 4588 5349 6377 1540 Size: 275 186 97 2490 761 1028 2301 Fragments arranged by size: 2490 2301 1028 761 275 186 97 BsiHKAI G_wGCw'C Cuts at: 3072 3144 3421 4299 5199 5284 6355 3072 Size: 72 277 878 900 85 1071 3855 Fragments arranged by size: 3855 1071 900 878 277 85 72 BsmI GAATG_Cn' Cuts at: 3200 3958 3974 4649 4742 3200 Size: 758 16 675 93 5596 Fragments arranged by size: 5596 758 675 93 16 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2085 3072 3144 3421 3694 4299 4457 Size: 399 295 987 72 277 273 605 158 Cuts at: 4457 5199 5284 6355 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 987 742 605 399 295 277 273 158 85 72 BspEI T'CCGG_A Cuts at: 6801 6801 Size: 7138 BspHI T'CATG_A Cuts at: 5027 6757 5027 Size: 1730 5408 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4493 844 Size: 3649 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2100 2418 3745 3805 5025 1394 Size: 429 277 318 1327 60 1220 3507 Fragments arranged by size: 3507 1327 1220 429 318 277 60 BsrDI GCAATG_nn' Cuts at: 65 1248 3756 5633 5807 65 Size: 1183 2508 1877 174 1396 Fragments arranged by size: 2508 1877 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5782 5969 1700 Size: 110 3972 187 2869 Fragments arranged by size: 3972 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7138 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7138 BssSI C'ACGA_G Cuts at: 5198 6210 5198 Size: 1012 6126 BstAPI GCAn_nnn'nTGC Cuts at: 794 3734 794 Size: 2940 4198 BstBI TT'CG_AA Cuts at: 3460 5942 3460 Size: 2482 4656 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7138 BstYI r'GATC_y Cuts at: 1972 4121 4468 4835 5220 5237 6689 6775 6787 Size: 2149 347 367 385 17 1452 86 12 Cuts at: 6787 7133 1972 Size: 346 1977 Fragments arranged by size: 2149 1977 1452 385 367 347 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2777 2777 Size: 7138 BtgI C'CryG_G Cuts at: 513 1065 2356 513 Size: 552 1291 5295 Fragments arranged by size: 5295 1291 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6399 Fragments arranged by size: 6399 683 56 BtsI GCAGTG_nn' Cuts at: 3334 4654 5448 5468 3334 Size: 1320 794 20 5004 Fragments arranged by size: 5004 1320 794 20 ClaI AT'CG_AT Cuts at: 1554 3624 1554 Size: 2070 5068 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7103 DraI TTT'AAA Cuts at: 3399 4161 4442 3399 Size: 762 281 6095 Fragments arranged by size: 6095 762 281 DraIII CAC_nnn'GTG Cuts at: 2288 2288 Size: 7138 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6145 809 Size: 507 4829 1802 Fragments arranged by size: 4829 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2019 2095 4422 Size: 54 1425 50 275 18 189 76 2327 Cuts at: 4422 4585 5474 7103 8 Size: 163 889 1629 43 Fragments arranged by size: 2327 1629 1425 889 275 189 163 76 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2095 4585 1537 Size: 275 283 2490 4090 Fragments arranged by size: 4090 2490 283 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3768 4173 5068 1353 Size: 2415 405 895 3423 Fragments arranged by size: 3423 2415 895 405 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3826 5725 6099 6245 387 Size: 1064 138 2237 1899 374 146 1280 Fragments arranged by size: 2237 1899 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3070 4297 3070 Size: 1227 5911 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2292 2555 2586 2774 2871 3056 4265 Size: 393 660 263 31 188 97 185 1209 Cuts at: 4265 4340 4419 5201 5798 6585 6853 1239 Size: 75 79 782 597 787 268 1524 Fragments arranged by size: 1524 1209 787 782 660 597 393 268 263 188 185 97 79 75 31 EcoRI G'AATT_C Cuts at: 4447 4825 4447 Size: 378 6760 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3410 3794 3410 Size: 384 6754 FspI TGC'GCA Cuts at: 5644 5644 Size: 7138 HaeII r_GCGC'y Cuts at: 1678 6285 1678 Size: 4607 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2245 2277 5779 5811 5853 Size: 32 135 32 673 32 3502 32 42 Cuts at: 5853 5885 1373 Size: 32 2626 Fragments arranged by size: 3502 2626 673 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2117 2455 3878 4482 4728 669 Size: 988 281 179 338 1423 604 246 3079 Fragments arranged by size: 3079 1423 988 604 338 281 246 179 HindIII A'AGCT_T Cuts at: 748 3087 3251 748 Size: 2339 164 4635 Fragments arranged by size: 4635 2339 164 HpaI GTT'AAC Cuts at: 4728 4728 Size: 7138 KpnI G_GTAC'C Cuts at: 4463 4463 Size: 7138 MfeI C'AATT_G Cuts at: 3605 4737 3605 Size: 1132 6006 MluI A'CGCG_T Cuts at: 7014 7014 Size: 7138 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2529 3022 6252 6436 1305 Size: 1224 493 3230 184 2007 Fragments arranged by size: 3230 2007 1224 493 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2021 4424 10 Size: 54 1425 343 189 2403 2724 Fragments arranged by size: 2724 2403 1425 343 189 54 MslI CAynn'nnrTG Cuts at: 518 1166 2751 5096 5455 5614 518 Size: 648 1585 2345 359 159 2042 Fragments arranged by size: 2345 2042 1585 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2112 2575 4154 4554 5231 6379 6624 1606 Size: 506 463 1579 400 677 1148 245 2120 Fragments arranged by size: 2120 1579 1148 677 506 463 400 245 NcoI C'CATG_G Cuts at: 513 1065 2356 513 Size: 552 1291 5295 Fragments arranged by size: 5295 1291 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7138 NheI G'CTAG_C Cuts at: 1052 3487 1052 Size: 2435 4703 NotI GC'GGCC_GC Cuts at: 4585 4585 Size: 7138 NsiI A_TGCA'T Cuts at: 3958 3958 Size: 7138 NspI r_CATG'y Cuts at: 1132 1229 2685 4496 6041 1132 Size: 97 1456 1811 1545 2229 Fragments arranged by size: 2229 1811 1545 1456 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7138 PciI A'CATG_T Cuts at: 6037 6037 Size: 7138 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7138 PfoI T'CCnGG_A Cuts at: 3639 3639 Size: 7138 PmeI GTTT'AAAC Cuts at: 4442 4442 Size: 7138 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3054 3086 5207 5239 6720 6752 3054 Size: 32 2121 32 1481 32 3440 Fragments arranged by size: 3440 2121 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7138 PsiI TTA'TAA Cuts at: 2243 4708 2243 Size: 2465 4673 PstI C_TGCA'G Cuts at: 830 4490 830 Size: 3660 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7138 PvuII CAG'CTG Cuts at: 4154 4154 Size: 7138 SacI G_AGCT'C Cuts at: 3072 4299 3072 Size: 1227 5911 SalI G'TCGA_C Cuts at: 1936 2115 4480 1936 Size: 179 2365 4594 Fragments arranged by size: 4594 2365 179 SapI GCTCTTCn'nnn_ Cuts at: 3768 3768 Size: 7138 SbfI CC_TGCA'GG Cuts at: 4490 4490 Size: 7138 ScaI AGT'ACT Cuts at: 3670 5386 7098 3670 Size: 1716 1712 3710 Fragments arranged by size: 3710 1716 1712 SfcI C'TryA_G Cuts at: 826 1045 2519 3339 4486 5621 6302 6493 826 Size: 219 1474 820 1147 1135 681 191 1471 Fragments arranged by size: 1474 1471 1147 1135 820 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7138 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7138 SmaI CCC'GGG Cuts at: 1872 4465 1872 Size: 2593 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3779 3800 4106 5242 6143 6405 Size: 197 926 1836 21 306 1136 901 262 Cuts at: 6405 820 Size: 1553 Fragments arranged by size: 1836 1553 1136 926 901 306 262 197 21 SnaBI TAC'GTA Cuts at: 493 4929 493 Size: 4436 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7138 SphI G_CATG'C Cuts at: 1132 2685 4496 1132 Size: 1553 1811 3774 Fragments arranged by size: 3774 1811 1553 SspI AAT'ATT Cuts at: 5 52 5062 5 Size: 47 5010 2081 Fragments arranged by size: 5010 2081 47 StuI AGG'CCT Cuts at: 1517 2528 1517 Size: 1011 6127 StyI C'CwwG_G Cuts at: 513 1065 2016 2356 4577 513 Size: 552 951 340 2221 3074 Fragments arranged by size: 3074 2221 951 552 340 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2349 2606 5165 5182 5335 5520 5965 Size: 1255 769 257 2559 17 153 185 445 Cuts at: 5965 325 Size: 1498 Fragments arranged by size: 2559 1498 1255 769 445 257 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2484 3668 4231 Size: 274 80 33 51 1443 507 1184 563 Cuts at: 4231 5384 7096 96 Size: 1153 1712 138 Fragments arranged by size: 1712 1443 1184 1153 563 507 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2313 2345 7098 7130 2313 Size: 32 4753 32 2321 Fragments arranged by size: 4753 2321 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7138 XbaI T'CTAG_A Cuts at: 4474 4474 Size: 7138 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7138 XmaI C'CCGG_G Cuts at: 1870 4463 1870 Size: 2593 4545 XmnI GAAnn'nnTTC Cuts at: 2818 5267 2818 Size: 2449 4689 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4946 276 Size: 53 83 186 936 3412 2468 Fragments arranged by size: 3412 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI