(Circular) (Six-base) MAPSORT of: pFN21AE1962.seq Check: 6118 from: 1 to: 6286 pFN21AE1962 6286 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4096 278 Size: 53 83 186 936 2560 2468 Fragments arranged by size: 2560 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3629 1937 Size: 1692 4594 Acc65I G'GTAC_C Cuts at: 3607 3607 Size: 6286 AclI AA'CG_TT Cuts at: 1563 3321 4413 4786 1563 Size: 1758 1092 373 3063 Fragments arranged by size: 3063 1758 1092 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2552 3041 3425 4349 5733 6001 1632 Size: 920 489 384 924 1384 268 1917 Fragments arranged by size: 1917 1384 924 920 489 384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6089 AflIII A'CryG_T Cuts at: 1203 5185 6162 1203 Size: 3982 977 1327 Fragments arranged by size: 3982 1327 977 AgeI A'CCGG_T Cuts at: 5117 5117 Size: 6286 AhdI GACnn_n'nnGTC Cuts at: 5015 5015 Size: 6286 AleI CACnn'nnGTG Cuts at: 1166 3539 1166 Size: 2373 3913 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2713 1184 Size: 1529 4757 AlwNI CAG_nnn'CTG Cuts at: 4029 5601 4029 Size: 1572 4714 ApaI G_GGCC'C Cuts at: 2142 2151 2142 Size: 9 6277 ApaLI G'TGCA_C Cuts at: 4343 5499 4343 Size: 1156 5130 ApoI r'AATT_y Cuts at: 1493 2208 2408 2702 3417 3595 3821 3973 6082 Size: 715 200 294 715 178 226 152 2109 Cuts at: 6082 1493 Size: 1697 Fragments arranged by size: 2109 1697 715 715 294 226 200 178 152 AseI AT'TA_AT Cuts at: 160 1032 4840 160 Size: 872 3808 1606 Fragments arranged by size: 3808 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6286 AvaI C'yCGr_G Cuts at: 1870 1943 3147 3611 1870 Size: 73 1204 464 4545 Fragments arranged by size: 4545 1204 464 73 BamHI G'GATC_C Cuts at: 2020 2473 2536 3616 3983 2020 Size: 453 63 1080 367 4323 Fragments arranged by size: 4323 1080 453 367 63 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3607 5062 618 Size: 325 217 627 1820 1455 1842 Fragments arranged by size: 1842 1820 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2142 2151 3605 1391 Size: 751 9 1454 4072 Fragments arranged by size: 4072 1454 751 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2327 928 Size: 971 428 4887 Fragments arranged by size: 4887 971 428 BbvCI CC'TCA_GC Cuts at: 2985 2985 Size: 6286 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3166 3200 4477 4511 1903 Size: 34 1229 34 1277 34 3678 Fragments arranged by size: 3678 1277 1229 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4178 5388 4178 Size: 1210 5076 BclI T'GATC_A Cuts at: 1548 1548 Size: 6286 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2146 4897 136 Size: 107 122 71 631 605 474 2751 1525 Fragments arranged by size: 2751 1525 631 605 474 122 107 71 BglII A'GATC_T Cuts at: 6281 6281 Size: 6286 BlpI GC'TnA_GC Cuts at: 3703 3703 Size: 6286 Bme1580I G_kGCm'C Cuts at: 1790 2142 2151 4347 5503 1790 Size: 352 9 2196 1156 2573 Fragments arranged by size: 2573 2196 1156 352 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6286 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2099 2440 2933 Size: 416 53 83 399 24 682 341 493 Cuts at: 2933 4017 4975 442 Size: 1084 958 1753 Fragments arranged by size: 1753 1084 958 682 493 416 399 341 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6286 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2535 2535 Size: 6286 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2112 2376 2581 3281 3303 4946 1239 Size: 873 264 205 700 22 1643 2579 Fragments arranged by size: 2579 1643 873 700 264 205 22 Bpu10I CC'TnA_GC Cuts at: 2985 2985 Size: 6286 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4411 5276 5574 4411 Size: 865 298 5123 Fragments arranged by size: 5123 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4949 882 Size: 617 3450 2219 Fragments arranged by size: 3450 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4077 493 Size: 711 2873 2702 Fragments arranged by size: 2873 2702 711 BsaBI GATnn'nnATC Cuts at: 2628 2650 6280 2628 Size: 22 3630 2634 Fragments arranged by size: 3630 2634 22 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4093 4475 5110 275 Size: 53 83 186 936 2560 382 635 1451 Fragments arranged by size: 2560 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2539 2961 3238 3548 4719 5117 5391 5538 5949 Size: 422 277 310 1171 398 274 147 411 Cuts at: 5949 2539 Size: 2876 Fragments arranged by size: 2876 1171 422 411 398 310 277 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2554 2584 1178 Size: 30 165 30 1151 30 4880 Fragments arranged by size: 4880 1151 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6286 BseYI C'CCAG_C Cuts at: 5489 5985 5489 Size: 496 5790 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3563 1163 Size: 573 1827 3886 Fragments arranged by size: 3886 1827 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3736 4497 5525 1540 Size: 275 186 1735 761 1028 2301 Fragments arranged by size: 2301 1735 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3148 3208 3238 3267 4347 4432 5503 3148 Size: 60 30 29 1080 85 1071 3931 Fragments arranged by size: 3931 1080 1071 85 60 30 29 BsmI GAATG_Cn' Cuts at: 3338 3529 3797 3890 3338 Size: 191 268 93 5734 Fragments arranged by size: 5734 268 191 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2142 2151 3148 3208 3238 3267 3605 Size: 399 352 9 997 60 30 29 338 Cuts at: 3605 4347 4432 5503 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 997 742 399 352 338 85 60 30 29 9 BspEI T'CCGG_A Cuts at: 2539 3238 3548 5949 2539 Size: 699 310 2401 2876 Fragments arranged by size: 2876 2401 699 310 BspHI T'CATG_A Cuts at: 2853 4175 5905 2853 Size: 1322 1730 3234 Fragments arranged by size: 3234 1730 1322 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3641 844 Size: 2797 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4173 1394 Size: 429 2350 3507 Fragments arranged by size: 3507 2350 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2808 3390 4781 4955 65 Size: 1183 1560 582 1391 174 1396 Fragments arranged by size: 1560 1396 1391 1183 582 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3395 4930 5117 1700 Size: 110 1585 1535 187 2869 Fragments arranged by size: 2869 1585 1535 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6286 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6286 BssSI C'ACGA_G Cuts at: 2391 4346 5358 2391 Size: 1955 1012 3319 Fragments arranged by size: 3319 1955 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6286 BstBI TT'CG_AA Cuts at: 5090 5090 Size: 6286 BstEII G'GTnAC_C Cuts at: 1184 2099 1184 Size: 915 5371 BstXI CCAn_nnnn'nTGG Cuts at: 2642 3281 2642 Size: 639 5647 BstYI r'GATC_y Cuts at: 1972 2020 2434 2473 2536 2773 2794 3551 3616 Size: 48 414 39 63 237 21 757 65 Cuts at: 3616 3983 4368 4385 5837 5923 5935 6281 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 757 414 385 367 346 237 86 65 63 48 39 21 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2143 513 Size: 552 939 139 4656 Fragments arranged by size: 4656 939 552 139 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2314 2615 3080 487 Size: 683 56 1088 301 465 3693 Fragments arranged by size: 3693 1088 683 465 301 56 BtsI GCAGTG_nn' Cuts at: 2409 3303 3802 4596 4616 2409 Size: 894 499 794 20 4079 Fragments arranged by size: 4079 894 794 499 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6286 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3181 3216 529 Size: 35 2617 35 3599 Fragments arranged by size: 3599 2617 35 35 DraI TTT'AAA Cuts at: 3590 3590 Size: 6286 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5293 809 Size: 507 3977 1802 Fragments arranged by size: 3977 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3153 3397 3733 Size: 54 1425 50 275 18 1323 244 336 Cuts at: 3733 4622 6251 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1323 889 336 275 244 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3733 1537 Size: 275 1921 4090 Fragments arranged by size: 4090 1921 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3495 4216 1353 Size: 2142 721 3423 Fragments arranged by size: 3423 2142 721 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4873 5247 5393 387 Size: 1064 138 3284 374 146 1280 Fragments arranged by size: 3284 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2112 2376 2552 2581 3041 3281 3303 Size: 393 480 264 176 29 460 240 22 Cuts at: 3303 3425 4349 4946 5733 6001 1239 Size: 122 924 597 787 268 1524 Fragments arranged by size: 1524 924 787 597 480 460 393 268 264 240 176 122 29 22 EcoO109I rG'GnC_Cy Cuts at: 2138 2139 2148 2244 2386 3104 2138 Size: 1 9 96 142 718 5320 Fragments arranged by size: 5320 718 142 96 9 1 EcoRI G'AATT_C Cuts at: 2208 3595 3973 2208 Size: 1387 378 4521 Fragments arranged by size: 4521 1387 378 FspI TGC'GCA Cuts at: 4792 4792 Size: 6286 HaeII r_GCGC'y Cuts at: 1678 5433 1678 Size: 3755 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2554 2586 2848 2880 3361 Size: 32 135 32 982 32 262 32 481 Cuts at: 3361 3393 4927 4959 5001 5033 1373 Size: 32 1534 32 42 32 2626 Fragments arranged by size: 2626 1534 982 481 262 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3630 3876 669 Size: 988 281 1692 246 3079 Fragments arranged by size: 3079 1692 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6286 HpaI GTT'AAC Cuts at: 3876 3876 Size: 6286 KpnI G_GTAC'C Cuts at: 3611 3611 Size: 6286 MfeI C'AATT_G Cuts at: 3885 3885 Size: 6286 MluI A'CGCG_T Cuts at: 6162 6162 Size: 6286 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2422 5400 5584 1305 Size: 1117 2978 184 2007 Fragments arranged by size: 2978 2007 1117 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3155 10 Size: 54 1425 343 1323 3141 Fragments arranged by size: 3141 1425 1323 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2640 3539 4244 4603 4762 518 Size: 648 1474 899 705 359 159 2042 Fragments arranged by size: 2042 1474 899 705 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2059 2372 3702 4379 5527 5772 1606 Size: 453 313 1330 677 1148 245 2120 Fragments arranged by size: 2120 1330 1148 677 453 313 245 NaeI GCC'GGC Cuts at: 3397 3397 Size: 6286 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4795 Fragments arranged by size: 4795 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6286 NgoMIV G'CCGG_C Cuts at: 3395 3395 Size: 6286 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6286 NotI GC'GGCC_GC Cuts at: 3733 3733 Size: 6286 NspI r_CATG'y Cuts at: 1132 1229 2231 3022 3187 3644 5189 1132 Size: 97 1002 791 165 457 1545 2229 Fragments arranged by size: 2229 1545 1002 791 457 165 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6286 PciI A'CATG_T Cuts at: 5185 5185 Size: 6286 PflMI CCAn_nnn'nTGG Cuts at: 1108 2910 3100 1108 Size: 1802 190 4294 Fragments arranged by size: 4294 1802 190 PfoI T'CCnGG_A Cuts at: 2790 2831 2790 Size: 41 6245 PmeI GTTT'AAAC Cuts at: 3590 3590 Size: 6286 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4355 4387 5868 5900 4355 Size: 32 1481 32 4741 Fragments arranged by size: 4741 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2386 3104 2386 Size: 718 5568 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6286 PsiI TTA'TAA Cuts at: 2712 3856 2712 Size: 1144 5142 PspOMI G'GGCC_C Cuts at: 2138 2147 2138 Size: 9 6277 PspXI vC'TCGA_Gb Cuts at: 3147 3147 Size: 6286 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3122 3154 3122 Size: 32 6254 PstI C_TGCA'G Cuts at: 830 3638 830 Size: 2808 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6286 PvuII CAG'CTG Cuts at: 2372 2372 Size: 6286 SalI G'TCGA_C Cuts at: 1936 3628 1936 Size: 1692 4594 SanDI GG'GwC_CC Cuts at: 2386 3104 2386 Size: 718 5568 SapI GCTCTTCn'nnn_ Cuts at: 3495 3495 Size: 6286 SbfI CC_TGCA'GG Cuts at: 3638 3638 Size: 6286 ScaI AGT'ACT Cuts at: 4534 6246 4534 Size: 1712 4574 SfcI C'TryA_G Cuts at: 826 1045 3634 4769 5450 5641 826 Size: 219 2589 1135 681 191 1471 Fragments arranged by size: 2589 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2146 2146 Size: 6286 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6286 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6286 SmaI CCC'GGG Cuts at: 1872 3613 1872 Size: 1741 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3147 4390 5291 5553 820 Size: 197 926 1204 1243 901 262 1553 Fragments arranged by size: 1553 1243 1204 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4077 493 Size: 3584 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6286 SphI G_CATG'C Cuts at: 1132 2231 3187 3644 1132 Size: 1099 956 457 3774 Fragments arranged by size: 3774 1099 956 457 SspI AAT'ATT Cuts at: 5 52 3278 4210 5 Size: 47 3226 932 2081 Fragments arranged by size: 3226 2081 932 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6286 StyI C'CwwG_G Cuts at: 513 1065 2004 2289 2904 3725 513 Size: 552 939 285 615 821 3074 Fragments arranged by size: 3074 939 821 615 552 285 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2214 4313 4330 4483 4668 5113 325 Size: 1255 634 2099 17 153 185 445 1498 Fragments arranged by size: 2099 1498 1255 634 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3501 4532 6244 Size: 274 80 33 51 1443 1524 1031 1712 Cuts at: 6244 96 Size: 138 Fragments arranged by size: 1712 1524 1443 1031 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6246 6278 6246 Size: 32 6254 Tth111I GACn'n_nGTC Cuts at: 1316 2546 1316 Size: 1230 5056 XbaI T'CTAG_A Cuts at: 3622 3622 Size: 6286 XhoI C'TCGA_G Cuts at: 1943 3147 1943 Size: 1204 5082 XmaI C'CCGG_G Cuts at: 1870 3611 1870 Size: 1741 4545 XmnI GAAnn'nnTTC Cuts at: 2877 4415 2877 Size: 1538 4748 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4094 276 Size: 53 83 186 936 2560 2468 Fragments arranged by size: 2560 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI RsrII SacI SacII SexAI SfoI SrfI SwaI XcmI