Restriction map for
pFN21AE1096
(Linear) (Six-base) MAPSORT of: KIEE1096.seq Check: 8658 from: 1 to: 1098
KIEE1096.orf
With 182 enzymes: SgfI *
November 19, 2009 09:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 154 1098
Size: 154 944
AfeI AGC'GCT
Cuts at: 0 407 421 1098
Size: 407 14 677
Fragments arranged by size:
677 407 14
AleI CACnn'nnGTG
Cuts at: 0 148 1098
Size: 148 950
ApaI G_GGCC'C
Cuts at: 0 530 1098
Size: 530 568
AseI AT'TA_AT
Cuts at: 0 1091 1098
Size: 1091 7
AvaI C'yCGr_G
Cuts at: 0 1067 1098
Size: 1067 31
BanI G'GyrC_C
Cuts at: 0 565 748 1098
Size: 565 183 350
Fragments arranged by size:
565 350 183
BanII G_rGCy'C
Cuts at: 0 530 1098
Size: 530 568
BbsI GAAGACnn'nnnn_
Cuts at: 0 666 1098
Size: 666 432
BlpI GC'TnA_GC
Cuts at: 0 409 1098
Size: 409 689
Bme1580I G_kGCm'C
Cuts at: 0 530 1098
Size: 530 568
BmrI ACTGGGnnnn_n'
Cuts at: 0 69 1098
Size: 69 1029
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 894 1098
Size: 894 204
Bpu10I CC'TnA_GC
Cuts at: 0 757 1098
Size: 757 341
BsaAI yAC'GTr
Cuts at: 0 840 1098
Size: 840 258
BsaHI Gr'CG_yC
Cuts at: 0 13 134 1098
Size: 13 121 964
Fragments arranged by size:
964 121 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 331 738 1098
Size: 331 407 360
Fragments arranged by size:
407 360 331
BseYI C'CCAG_C
Cuts at: 0 16 87 1098
Size: 16 71 1011
Fragments arranged by size:
1011 71 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 360 1098
Size: 360 738
BsiHKAI G_wGCw'C
Cuts at: 0 395 557 1098
Size: 395 162 541
Fragments arranged by size:
541 395 162
Bsp1286I G_dGCh'C
Cuts at: 0 395 530 557 1098
Size: 395 135 27 541
Fragments arranged by size:
541 395 135 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 154 191 1098
Size: 154 37 907
Fragments arranged by size:
907 154 37
BsrFI r'CCGG_y
Cuts at: 0 317 1098
Size: 317 781
BsrGI T'GTAC_A
Cuts at: 0 965 1098
Size: 965 133
BssSI C'ACGA_G
Cuts at: 0 556 916 1098
Size: 556 360 182
Fragments arranged by size:
556 360 182
BstBI TT'CG_AA
Cuts at: 0 1019 1098
Size: 1019 79
BstEII G'GTnAC_C
Cuts at: 0 462 1098
Size: 462 636
BstXI CCAn_nnnn'nTGG
Cuts at: 0 150 198 459 1098
Size: 150 48 261 639
Fragments arranged by size:
639 261 150 48
BtgI C'CryG_G
Cuts at: 0 386 506 1098
Size: 386 120 592
Fragments arranged by size:
592 386 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 156 749 1098
Size: 156 593 349
Fragments arranged by size:
593 349 156
BtsI GCAGTG_nn'
Cuts at: 0 1004 1098
Size: 1004 94
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 588 623 1098
Size: 588 35 475
Fragments arranged by size:
588 475 35
EaeI y'GGCC_r
Cuts at: 0 47 1098
Size: 47 1051
EarI CTCTTCn'nnn_
Cuts at: 0 17 115 163 358 937 1098
Size: 17 98 48 195 579 161
Fragments arranged by size:
579 195 161 98 48 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 735 1098
Size: 735 363
EcoNI CCTnn'n_nnAGG
Cuts at: 0 756 1098
Size: 756 342
FspI TGC'GCA
Cuts at: 0 223 1098
Size: 223 875
HaeII r_GCGC'y
Cuts at: 0 409 423 524 1098
Size: 409 14 101 574
Fragments arranged by size:
574 409 101 14
MslI CAynn'nnrTG
Cuts at: 0 148 196 535 634 1098
Size: 148 48 339 99 464
Fragments arranged by size:
464 339 148 99 48
MspA1I CmG'CkG
Cuts at: 0 123 1098
Size: 123 975
NspI r_CATG'y
Cuts at: 0 288 1098
Size: 288 810
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 16 48 1098
Size: 16 32 1050
Fragments arranged by size:
1050 32 16
PspOMI G'GGCC_C
Cuts at: 0 526 1098
Size: 526 572
PstI C_TGCA'G
Cuts at: 0 107 520 1098
Size: 107 413 578
Fragments arranged by size:
578 413 107
SapI GCTCTTCn'nnn_
Cuts at: 0 17 115 1098
Size: 17 98 983
Fragments arranged by size:
983 98 17
ScaI AGT'ACT
Cuts at: 0 907 1098
Size: 907 191
SfcI C'TryA_G
Cuts at: 0 103 516 693 797 1098
Size: 103 413 177 104 301
Fragments arranged by size:
413 301 177 104 103
SphI G_CATG'C
Cuts at: 0 288 1098
Size: 288 810
StyI C'CwwG_G
Cuts at: 0 452 467 574 881 1098
Size: 452 15 107 307 217
Fragments arranged by size:
452 307 217 107 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 633 736 1098
Size: 633 103 362
Fragments arranged by size:
633 362 103
TatI w'GTAC_w
Cuts at: 0 905 965 1098
Size: 905 60 133
Fragments arranged by size:
905 133 60
Enzymes that do cut:
AarI AfeI AleI ApaI AseI AvaI BanI BanII
BbsI BlpI Bme1580I BmrI BplI Bpu10I BsaAI BsaHI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI BsrGI
BssSI BstBI BstEII BstXI BtgI BtgZI BtsI CspCI
EaeI EarI EciI EcoNI FspI HaeII MslI MspA1I
NspI PpiI PspOMI PstI SapI ScaI SfcI SphI
StyI TaqII TatI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaLI ApoI AscI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BmgBI BmtI BpmI BpuEI BsaI
BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BssHII BstAPI BstYI BstZ17I Bsu36I
ClaI DraI DraIII DrdI EagI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FseI FspAI Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpuMI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StuI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI